Buried seed populations in the montane, subalpine, and alpine belts of Mont Jacques-Cartier, Quebec

1988 ◽  
Vol 66 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Hubert Morin ◽  
Serge Payette

The analysis of the total buried seed population along an altitudinal gradient in the Mont Jacques-Cartier area, southern Quebec, reveals a close relationship between buried seed flora and aboveground vegetation. Eighty-one percent of the species present in the total buried seed population were present in the aboveground vegetation of the study sites. The total number of seeds in the buried seed population, the number of species in the total buried seed population, and the number of seeds and species in the seed bank did not show any significant linear correlation with altitude. A shift from a boreal to an arctic–alpine buried seed population with increasing altitude was observed, instead of a decrease in seed bank size.

2000 ◽  
Vol 78 (6) ◽  
pp. 728-747 ◽  
Author(s):  
Ulf Molau ◽  
Eva-Lena Larsson

We studied the seed flux, including seed rain and seed bank (germinable and total), at twelve sites along an altitudinal gradient in the Abisko area in northernmost Swedish Lapland during a period of 3 years with contrasting summer climates. The study sites were evenly spaced in altitude from the timberline at 700 m above sea level to the highest peaks in the area (1560 m). A subalpine birch forest site was included for comparison. Each site was equipped with seed traps, replaced and emptied directly upon snow-melt each summer. Soil samples for seed bank assessment were taken at all sites, and inventories of the vascular plant flora were carried out in the 10 m radius neighborhood of the traps. The results revealed high variation among years with regard to seed rain and its partitioning over various functional types of plants. Even though most of the seed rain could be attributed to species present in the plant community of the trap sites themselves, some more long-distance dispersal takes place every year. A number of extrazonal recoveries are reported, often several hundred m above the distributional limit of the species. Even though seed number and species diversity declined rapidly from seed rain over total seed bank to germinable seed bank, the correlation among all three aspects of the seed pool was high. The dominant species in the seed flux at moderate altitudes, Empetrum hermaphroditum Hagerup, has a persistent seed bank with an average turnover of more than 200 years.Key words: seed rain, seed bank, total seed bank, germinable seed bank, alpine, dispersal.


2005 ◽  
Vol 15 (3) ◽  
pp. 239-248 ◽  
Author(s):  
Lukáš Krinke ◽  
Lenka Moravcová ◽  
Petr Pyšek ◽  
Vojtěch Jarošík ◽  
Jan Pergl ◽  
...  

The seed bank of Heracleum mantegazzianum, native to the western Caucasus and invasive in Europe, was studied in the Slavkovský les Protected Landscape Area, the Czech Republic, during the course of two growing seasons. In each of seven study sites with dominating stands of the species, soil samples were taken in October (after the fruit release), April (before germination) and June–July (before the fruit release). Ten samples were taken from each site using a soil hand bore, 4.6 cm in diameter. Seeds elutriated from the samples were immediately germinated in laboratory conditions (10°C, 12 h light/12 h dark); those that germinated during 1 month were considered as non-dormant. Non-germinated but viable seeds, tested for viability by tetrazolium, were considered as dormant. The number of dead seeds was also recorded. The total number of seeds significantly increased with mean density of flowering plants at a site. Of the total variation in seed-bank size, about four-fifths were attributed to that among sites, and one-fifth to that within sites. The number and proportion of living seeds differed significantly between years in summer, but not in autumn and spring. Total number and the numbers of dormant, non-dormant, living and dead seeds significantly differed among the autumn, spring and summer samples. The percentage of living seeds in the total seed bank decreased from 56% in autumn to 42% in spring to 15% in summer. The percentage of non-dormant seeds among those living was 0.3% in autumn, it increased to 87.5% in the spring sample, and decreased to 3% in summer. Pooled across all sites, the average seed numbers (expressed per m2) were 6719±4119 (mean±SD) in autumn, 4907±2278 in spring and 1301±1036 in summer for the total number of seeds, and 3759±2906, 2044±1198 and 192±165, respectively, for living seeds. The majority of seeds (95%) were concentrated in the upper 5 cm soil layer. However, some seeds were present in lower soil layers, which indicates a short-term persistent seed bank. The present data are the first quantitative estimate of the seed bank in H. mantegazzianum, and show that the reproductive potential of the species is enormous, which seems to be a crucial feature of its invasion success.


2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S110-S110
Author(s):  
A Vijayanarayanan ◽  
K Inamdar ◽  
M Menon ◽  
P Kuriakose

Abstract Introduction/Objective Myeloma diagnosis by a pathologist requires 10% plasma cells (PC) or a biopsy proven plasmacytoma in addition to myeloma defining events. PC% > 60% is a biomarker of malignancy under this definition. WHO allows for assesment of plasma cell percentage either by aspirate count or by CD138 immunohistochemistry (IHC). There is lack of consensus on aspirate smear adequacy for PC% estimation. Uneven distribution of plasma cells, hemodilution and/or patchy infiltration can lead to gross underestimation. We compared PC% by aspirate count and CD138 IHC and established corelation with serum protein electrophoresis (SPEP) values. Methods 67 myeloma cases were included after excluding cases with suboptimal or inadequate aspirate smears. Two hematopathologists evaluated the diagnostic marrow (therapy naive) for PC% by aspirate count and CD138 IHC on biopsy/clot section. Corresponding SPEP and Free light chain (FLC) values were obtained. Correlation coefficent was calculated using Pearson correlation coefficient (GraphPad Prism). Results The Ig subtypes included IgG (41/67) and IgA (17/67). 12 cases had available FLC values. Both average and median PC% by CD138 IHC was considerably higher (50%, 52%) compared to aspirate count (29%, 21%). However, PC% by aspirate smear count and CD138 IHC demonstrated a significant linear correlation (r=0.71, p60% by CD138 (and not by aspirate count). Conclusion CD138 IHC based PC% is consistently higher, nevertheless, statistically significant linear corelation is observed between aspirate count PC% and CD138 IHC. A significant linear correlation is observed between CD138 IHC and SPEP (IgG and IgA), however, no such correlation is observed with aspirate count. More cases were diagnosed as myeloma (11%) and higher propotion of cases (35%) had biomarker of malignancy i.e. PC% >60% by CD138 IHC. Based on these findings, we propose estimation of PC% by CD138 immunostain be a recommended standard practice for better clinicopathologic and biologic correlation.


Prominent ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 45-53
Author(s):  
Ernis Sholikah ◽  
Suprihadi Suprihadi ◽  
Nuraeningsih Nuraeningsih

Higher-order thinking skills (HOTS) is a new concept of thinking in Curriculum 2013 in Indonesia, which is based on the revised top three levels in Bloom’s Taxonomy: analyzing, evaluating, and creating. This survey research used 44 students who were purposively sampled. The objective of this research was to determine if there was a linear correlation between HOTS and the English achievement of the eleventh grade students of SMA N 2 Kudus. Using Pearson’s Product Moment Correlation in the level of significance .05 and degree of freedom 42, this research showed that there was a high positive significant linear correlation between HOTS and the English achievement of the eleventh grade students of SMA N 2 Kudus. Key words: HOTS, taxonomy, curriculum 2013, English achievement


2021 ◽  
Vol 32 (1) ◽  
pp. 94-101
Author(s):  
A. O. Jolaosho ◽  
J. A. Olanite ◽  
B. O. Oduguwa ◽  
E. A. O. Adekunle

An experiment to determine the effects of seasonal variations on population and viability of seeds in the faeces of ruminant animals was conducted between April to December 2001 using two breeds of cattle, sheep and goats. Three samples were collected per breed from three animals that were tagged for uniformity of data collection. Sampling took place weekly, in the mornings before the animals were taken out for grazing. The highest (P<0.05) total number of seeds, seeds/g dry weights and total number of viable seeds were recovered from cattle, while the least were from goats, but the reverse was the case for percentage viability. The weights of the faeces were significantly (P<0.05) higher in the rainy season than the dry season. The weights of the faeces reduced gradually as the dry season sets in. Likewise, there were more seeds in the early rain than in the late rain and early dry season, with the percentage viability higher in the late rains and early dry season than the early rainy season. There were more broadleaved weeds and sedges in the early rain than late rain and early dry season but the reverse was the case for grasses. In conclusion, although there were more faeces and consequently more seed production in the rainy season, however, but the viability was lower than in the dry season. Also, seeds of broadleaved plants were more in the rainy season while those of grasses were more in the dry season. More seeds and number of viable seeds were recovered from the faeces of cattle than sheep and goats in all the seasons due to the higher weights of faeces but the percentage viability was higher for sheep and goat than cattle. 


Circulation ◽  
2019 ◽  
Vol 140 (Suppl_2) ◽  
Author(s):  
Ingvild B Tjelmeland ◽  
Morten Larsen ◽  
Eirik Skogvoll ◽  
Jo Kramer-Johansen

Purpose of the study: The Norwegian Cardiac Arrest Registry (NorCAR) is a mandatory national registry of resuscitation attempts that monitors the population incidence, care and outcome for cardiac arrest. Previously, substantial differences in the number of included patients per 100 000 inhabitants were observed in some “atypical” health trusts (HT). It was not known if this is a problem with data collection or reflected genuine population differences. The purpose of this study was to identify all patients that fill NorCAR’s inclusion criteria among these HTs, by imposing consistent methods for data collection. Materials and methods: The registry implemented consistent, standardised and rigorous data collection methods including targeted audits in four “atypical” health trusts, two with a high and two with a low reported population incidence. Registrations from January to March 2019 was compared with similar results from 2018. Results: In 2018, the observed nationwide annual incidence of out-of-hospital cardiac arrest was 64 per 100 000 inhabitants, varying from 40 to 102. At the four study sites the observed population incidence was stable among HT with high incidence (88 and 110) but apparently increased in the two HTs with previous low incidence after implementation of standardised collection method (from 48 to 56, and 46 to 54). Conclusions: The previously “atypical” low incidence probably reflected a problem with data collection. To achieve full coverage of the population, all data collectors need to collect data using the same method with a concise and thorough investigation of ambulance-, air ambulance-, dispatch-, and hospital records. Data collectors need to have a close relationship with ambulance and dispatch, and constant reminders and feedback on reported results are important. Implementing a new method for data collection takes time, and results need to be evaluated over time.


1997 ◽  
Vol 39 (6) ◽  
pp. 313-318 ◽  
Author(s):  
Julia Maria COSTA-CRUZ ◽  
Carina Barbosa BULLAMAH ◽  
Maria do Rosário F. GONÇALVES-PIRES ◽  
Dulcinéa Maria B. CAMPOS ◽  
Miguel Alípio VIEIRA

Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p <FONT FACE="Symbol">£</font> 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended


Land ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 533
Author(s):  
Eva Čížková ◽  
Jana Navrátilová ◽  
Stanislav Martinát ◽  
Josef Navrátil ◽  
Ryan J. Frazier

The near elimination of inland salt marshes in Central Europe occurred throughout the 19th and 20th centuries, and the currently remaining marshes exist in a degraded condition. This work examines the impact of groundwater level on the growth of plants from a seed bank obtained from a degraded salt marsh in proximity to still existing one through an ex-situ experiment. An experimental tank was set up with the sample seed bank experiencing differing levels of water level. There were 1233 specimens of 44 taxa grown from the seed bank, of which 5 species were abundant, and 10 species are considered as halophytes. Only Lotus tenuis from halophytes was more abundant, and only five species of halophytes were represented by more than three individuals. The water level has a significant impact on the number of species (based on linear regression analysis) as well as species distribution among different water level treatments (a non-metric multidimensional analysis (nMDS) followed by linear regression). The results show a strong negative relationship between the average water level and the number of species. The water level did not affect the species composition of halophytes, but differences in individual species abundances were found among the halophytes. The species Bupleurum tenuissimum, Crypsis schoenoides, Melilotus dentatus, and Plantago maritima grew on the drier and non-inundated soils. Tripolium pannonicum, Spergularia maritima, and Lotus tenuis grew on both wet and dry soils. Trifolium fragiferum and Bolboschoenus maritimus were found in places with water stagnant at the soil level. Pulicaria dysenterica grew in inundated soil.


2008 ◽  
Vol 30 (2) ◽  
pp. 100-110 ◽  
Author(s):  
Fernanda Costa Maia ◽  
Manoel de Souza Maia ◽  
Renée M. Bekker ◽  
Rogério Previatti Berton ◽  
Leandro Sebastião Caetano

The objective of the study was to characterize annual ryegrass seed population dynamics, managed for natural re-sowing, in no til systems in rotation with soybean, in different chronosequences An area was cultivated for two years with soybean, left as fallow land for the next two years and then cultivated again with soybean for the next two years. The four chronosequences represented different management periods, two with soybean (6 and 8 years old) and the other two resting (3 and 9 years old). Soil samples were taken every month during one year and divided into two depths (0-5 and 5-10 cm). Vegetation dynamics were also evaluated (number of plants, inflorescences and seedlings). Soil seed bank (SSB) dynamics showed structural patterns in time, with a "storage period" in summer, an "exhausting period" during autumn and a "transition period" in winter and spring. Pasture establishment by natural re-sowing was totally dependent on the annual recruitment of seeds from the soil. The influence of the management practices on the SSB was more important than the number of years that these practices had been implemented. Places where soybean was sown showed the largest SSBs. Most of the seeds overcame dormancy and germinated at the end of the summer and beginning of the autumn, showing a typically transitory SSB, but with a small proportion of persistent seeds


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