Preliminary identification of date palm cultivars by esterase isoenzymes and peroxidase activities

1988 ◽  
Vol 66 (1) ◽  
pp. 89-93 ◽  
Author(s):  
M. Baaziz ◽  
M. Saaidi
Keyword(s):  
Peroxidase Activity ◽  
Date Palm ◽  
Polyacrylamide Gel Electrophoresis ◽  
Phoenix Dactylifera ◽  
Acetone Powder ◽  
Practical Application ◽  
Preliminary Identification ◽  
Phoenix Dactylifera L ◽  
Bayoud Disease ◽  
Relationship Of

Two types of crude extracts were obtained from 10 cultivars of date palm (Phoenix dactylifera L.); the Tris buffer extracts from acetone powder were assayed for peroxidase activity and the phosphate buffer extracts were subjected to polyacrylamide gel electrophoresis. The gels were stained for esterase activity using α-naphthylacetate and α-naphthylbutyrate as substrates. The cultivars were divided into four sets on the basis of their esterase isoenzyme phenotypes. These sets were further separated into their individual cultivars on the basis of peroxidase activities. The results are discussed with respect to the possible relationship of the peroxidase activity in the vascular fusariosis of date palm (Bayoud disease). This study has potential for practical application; however, some additional refinements in procedures appear necessary to reduce extraction time.

scholarly journals Salt and drought stress exhibits oxidative stress and modulated protein patterns in roots and leaves of date palm (Phoenix dactylifera L.)

2021 ◽  
Vol 117 (1) ◽  
pp. 1
Author(s):  
Hussein J. SHAREEF ◽  
Jameel M. AL-KHAYRI
Keyword(s):  
Polyethylene Glycol ◽  
Date Palm ◽  
Protein Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Phoenix Dactylifera ◽  
Soluble Carbohydrate ◽  
Salinity Treatment ◽  
Protein Patterns ◽  
Drought Treatment ◽  
Phoenix Dactylifera L

<p>The formation of new proteins under the influence of harsh environmental conditions is a plant adaptation reaction. Two-year-old date palm tissue culture-derived plants from ‘Barhee’ grown in the field were subjected to salt stress (70 g l<sup>-1</sup> NaCl) and dehydration-induced by applying 70 g l<sup>-1</sup> polyethylene glycol or without irrigation and withholding irrigation (0 g l<sup>-1</sup>) for one month. The soluble carbohydrate content increased in response to salinity and polyethylene glycol treatment in leaves compared to the control and drought treatment without irrigation. Proline increased in all treatments. Malondialdehyde and hydrogen peroxide increased under salinity. Salinity treatment increased the activity of ascorbate peroxidase and catalase enzyme. Salinity and polyethylene glycol treatments increased abscisic acid, whereas the indoleacetic acid level decreased. The protein pattern of roots and leaves in one-dimensional polyacrylamide gel electrophoresis showed that the stress conditions led to new protein bands' appearance and other proteins' disappearance. A comparison of protein patterns between the control and stress treatments revealed that the relative intensity of proteins in roots and leaves were more associated with salinity treatment than the drought. The results may be clearing important the molecular mechanism of tolerance under the influence of extreme environmental stress.</p>

scholarly journals Biocontrol of Bayoud Disease (Fusarium oxysporum F. Sp. Albedinis) on Deglet-nour Variety of Date Palm (Phoenix dactylifera L.) in South Western Oases of Algeria

2021 ◽  
Author(s):  
Hakima Belaidi ◽  
Fawzia Toumi-Benali ◽  
Ibrahim Elkhalil Benzohra
Keyword(s):  
Fusarium Oxysporum ◽  
Date Palm ◽  
Total Mortality ◽  
Phoenix Dactylifera ◽  
Antagonistic Fungi ◽  
Palm Trees ◽  
Phoenix Dactylifera L ◽  
Complete Resistance ◽  
Bayoud Disease

Background: This work has the objective to biocontrol the bayoud disease of date palm caused by the fungus Fusarium oxysporum f. sp. albedinis (Foa), is the major disease in Algerian palm groves of south western region. This in vivo biocontrol was done on date palm variety Deglet-Nour, using two antagonistic fungi species, Trichoderma harzianum and Aspergillus flavus.Methods: Twenty Foa isolates obtained from isolation of the spines carrying the typical symptoms of Bayoud disease were used. Two strains T. harzianum and A. flavus, were isolated from rhizosphere soils of the date palm trees. Using the greenhouse screening test, which was carried out on 3-4 month-old date palm seedlings at the rate of 5 ml of inoculum suspension.Result: A statistical analysis showed a significant (P less than 0.05), difference of Deglet-Nour seedlings reactions against Foa isolates and antagonistic fungi were observed. All date palm trees present susceptibility against Foa until the total mortality of seedlings. The biocontrol test showed that two antagonists showed different reaction, with the complete resistance for seedlings treated by T. harzianum with the mortality rate (rm%) reduced at 100%, while, the second antagonist A. flavus reduced the rm% at 25%. We can apply of these antagonistic fungi to protect our groves contaminated by Bayoud disease and also contain this susceptible commercial variety.

Effect of date palm (Phoenix dactylifera L.) spikelets extract on the physicochemical and microbial properties of set-type yogurt during cold storage

LWT ◽  
2021 ◽  
pp. 111762
Author(s):  
Ibrahim A. Almusallam ◽  
Isam A. Mohamed Ahmed ◽  
Elfadil E. Babiker ◽  
Fahad Y. Al-Juhaimi ◽  
Ali Saleh ◽  
...  
Keyword(s):  
Cold Storage ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Microbial Properties ◽  
Phoenix Dactylifera L
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