Backscattered electron imaging as a tool for histochemically localizing calcium oxalate with the scanning electron microscope

1987 ◽  
Vol 65 (5) ◽  
pp. 888-892 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Acetic Acid ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Calcium Oxalate ◽  
Leaf Tissues ◽  
Backscattered Electron ◽  
Staining Techniques ◽  
Electron Imaging ◽  
First Time ◽  
Scanning Electron

The scanning electron microscope and a backscattered electron detector were used for the first time to identify calcium oxalate in leaf tissues after labelling crystals with silver. Freehand sections fixed in formalin – acetic acid – alcohol or glutaraldehyde were treated with 5% acetic acid to remove background calcium carbonate and calcium phosphate and were silver stained by calcium-substitution techniques. Two standard staining techniques, Pizzolato (AgNO3–H2O2) and Yasue (AgNO3 – rubeanic acid) tests, were compared. Druses and raphides were identified morphologically and histochemically in the leaves of Anthurium scherzeranum and Dieffenbachia maculata. The Pizzolato procedure was the best method for staining calcium oxalate for scanning electron microscope studies. Excessive deposition of granular material in the Yasue staining method tended to obscure crystal morphology in scanning electron microscope preparations.

Backscattered Electron Imaging in the Scanning Electron Microscope: the Use of Either: (a) High Incident Energy or (b) an Array Detector

2008 ◽  
Vol 14 (S2) ◽  
pp. 120-121
Author(s):  
LM Gignac ◽  
OC Wells ◽  
C-K Hu ◽  
J Bruley ◽  
CE Murray ◽  
...  
Keyword(s):  
Electron Microscope ◽  
New Mexico ◽  
Scanning Electron Microscope ◽  
Incident Energy ◽  
Array Detector ◽  
Backscattered Electron Imaging ◽  
High Incident Energy ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope.

1987 ◽  
Vol 35 (2) ◽  
pp. 267-269 ◽  
Author(s):  
D Soligo ◽  
E P de harven
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Cell Types ◽  
Myeloperoxidase Activity ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Simultaneous Visualization ◽  
Scanning Electron

We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

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