Dormance, germination et contenu protéique des semences de deux populations de Schoenus nigricans cueillies à des altitudes différentes

1987 ◽  
Vol 65 (4) ◽  
pp. 617-621 ◽  
Author(s):  
Emanuele Bocchieri ◽  
Giovanni Floris ◽  
Bonaria Mulas

The germination of seeds of two populations of Schoenus nigricans L. collected at two middle-west Sardinian sites at 5 and at 800 m above sea level were studied. Germination took place in the dark, in deionized water, at 10, 20, or 30 °C or at alternating temperatures (10:20 °C) with a 12-h thermoperiod. The tests of 60-day duration, demonstrated that 30 °C is an inhibiting temperature for the seeds here considered, while the alternating (10:20 °C) temperatures increase the germination compared with the constant (10 and 20 °C) conditions. This different behaviour was analysed with respect to the dormancy and germination of the seeds collected at the two stations. We suggest that, beyond the habitat factors, the morphological difference between the seeds are responsible for the results presently considered. The remarkable qualitative and quantitative differences concern mostly the protein content in the two seed populations. The environmental characteristics of the collecting sites of Schoenus nigricans L. are also discussed in detail.

1993 ◽  
Vol 104 (3) ◽  
pp. 811-818 ◽  
Author(s):  
M. Pypaert ◽  
T. Nilsson ◽  
E.G. Berger ◽  
G. Warren

HeLa cells were incubated with 15 nm BSA-gold for 1 or 2 hours to mark the endocytic pathway and mitotic cells were then isolated by shake-off. Thin, frozen sections were labelled with antibodies against two resident Golgi markers, beta-(1,4)-galactosyltransferase and N-acetylglucosaminyltransferase I. Detection of the latter was aided by the use of a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. The secondary antibodies were coupled to either 5 or 10 nm gold so that the distribution of each of the three markers could be followed. Qualitative and quantitative studies showed that there were two populations of clusters, those described by us earlier and termed Golgi clusters (Lucocq et al. (1987) J. Cell Biol. 104, 865–874), containing either or both Golgi markers, and clusters of tubular endosomes containing BSA-gold. There was very little overlap showing that Golgi clusters cannot be tubular endosomes as concluded by Tooze and Hollinshead (1992) Eur. J. Cell Biol. 58, 228–242.


1977 ◽  
Vol 32 (5-6) ◽  
pp. 405-408 ◽  
Author(s):  
O. Schmut ◽  
H. Katschnig ◽  
M. Zirm

Abstract Fluids of the human body with low protein content, i.e. aqueous humour, tears, cerebrospinal fluid, and urine were analyzed by a combination of micro-disc-electrophoresis and electroimmuno-diffusion. By this method both qualitative and quantitative statements about the proteins of these fluids can be established.


Zootaxa ◽  
2011 ◽  
Vol 2988 (1) ◽  
pp. 37 ◽  
Author(s):  
GRAŻYNA SOIKA ◽  
MARCIN KOZAK

The purpose of this research was to investigate both the qualitative and quantitative morphological traits of Phytoptus tetratrichus Nalepa 1890 populations inhabiting three different lime tree species: Tilia cordata Mill., Tilia tomentosa Moench and Tilia americana L.. Morphological characters of two populations collected from T. cordata and T. tomentosa over three successive growing seasons were compared with the aid of canonical variate analysis. Additionally, individuals occurring on T. americana in a consecutive year were also studied. Protogyne and deutogyne females were differentiated using both qualitative and quantitative traits. For deutogyne females, individuals from all combinations of Tilia species × year (which constituted populations for comparison) clearly differed from each other. However, the differences between populations from T. cordata and T. tomentosa were less distinct. For protogyne females, observed differences were clearly visible. The between-season variation in morphological characters such as body size appeared to be quite large, indicating that morphological analysis based on observations from only a single season can be inaccurate. Deutogyne females of P. tetratrichus were observed to cause various types of damage symptoms: leaf-roll galls along the leaf edges of T. cordata; small round erinea on the lower leaf surface and small wart-like galls on the upper leaf surface of T. tomentosa; fingerlike galls on both leaf surfaces of T. americana.


ZooKeys ◽  
2020 ◽  
Vol 913 ◽  
pp. 89-126 ◽  
Author(s):  
Royce T. Cumming ◽  
Sarah Bank ◽  
Stephane Le Tirant ◽  
Sven Bradler

Within the last two years, the leaf insects of the genus Phyllium of both the islands of Java and Sumatra have been reviewed extensively based on morphological observations. However, cryptic species which cannot be differentiated morphologically may be present among the various populations. Since it has frequently been demonstrated that analyses based on molecular data can bring clarity in such cases, we conducted a phylogenetic analysis based on three genes (nuclear gene 28S and mitochondrial genes COI and 16S) from the Phyllium species of these islands. The results show distinct molecular divergence for several populations and suggest the presence of two new cryptic species, morphologically inseparable from Phyllium hausleithneri Brock, 1999. From Sumatra, the population originally thought to be a range expansion for Phyllium hausleithneri, is now here described as Phyllium nisussp. nov., with the only consistent morphological difference being the color of the eggs between the two populations (dark brown in P. hausleithneri and tan in P. nisussp. nov.). Further, an additional population with purple coxae from Java was morphologically examined and found to have no consistent features to separate it morphologically from the other purple coxae species. This cryptic species from Java was however shown to be molecularly distinct from the other purple coxae populations from Sumatra and Peninsular Malaysia and is here described as Phyllium gardabagusisp. nov. In addition, Phyllium giganteum is here officially reported from Java for the first time based on both historic and modern records of male specimens.


2019 ◽  
Vol 56 (1) ◽  
pp. 1-10 ◽  
Author(s):  
M. S. B. Oliveira ◽  
E. Aparecido Adriano ◽  
M. Tavares-Dias ◽  
L. Lima Corrêa

SummaryThis study compared the monogeneans community in C. monoculus from the Tapajós River (state of Pará) and Jari River (state of Amapá), northern Brazil. A total of 2188 monogeneans belonging to eight taxa were collected from the gills of fish: Gussevia arilla, Gussevia longihaptor, Gussevia tucunarense, Gussevia undulata, Sciadicleithrum ergensi, Sciadicleithrum umbilicum, Sciadicleithrum uncinatum and Tucunarella cichlae. Gussevia arilla was the dominant species for C. monoculus from the Tapajós River basin, while S. umbilicum predominated among the hosts from the Jari River basin. For the two populations of C. monoculus, the prevalence, mean intensity and mean abundance of monogeneans were different and the of parasites community had a high qualitative similarity (87.5 %). The monogeneans community of C. monoculus was characterized by high species richness, with infection values varying from low to moderate. The geographic distance and differences in environmental characteristics arising from the same did not influence the richness of species of monogeneans infesting C. monoculus in the Tapajós and Jari rivers, but appear to have been determinants in the differences observed in the structure of the monogenean communities in each region.


Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 893
Author(s):  
Kavita Sharma ◽  
Neha Chanana ◽  
Ghulam Mohammad ◽  
Tashi Thinlas ◽  
Mohit Gupta ◽  
...  

Thrombospondin-1 (THBS1) levels elevate under hypoxia and have relevance in several cardiovascular disorders. The association of THBS1 with endothelial dysfunction implies its important role in hypertension. To establish the hypothesis, we screened patients with hypertension and their respective controls from the two different environmental regions. Cohort 1 was composed of Ladakhis, residing at 3500 m above sea level (ASL), whereas Cohort 2 was composed of north-Indians residing at ~200 m ASL. Clinical parameters and circulating THBS1 levels were correlated in the case–control groups of the two populations. THBS1 levels were significantly elevated in hypertension patients of both cohorts; however, the levels were distinctly enhanced in the hypertensive patients of HA as compared to normoxia (p < 0.002). The observation was supported by the receiver operating curve analysis with an area under curve of 0.7007 (0.627–0.774) demonstrating the discriminatory effect of hypobaric hypoxia on the levels as compared to normoxia (p < 0.011). Significant correlation of THBS1 and mean arterial pressure was observed with upraised positive correlations in the hypertensive highlanders as compared to the hypertensive patients from sea-level. The prevalence of differential distribution of THBS1 and CD47 genes variants, their interactions, and association with the THBS1 levels were also determined. Genotype-interactions between THBS1 rs2228263 and CD47 rs9879947 were relevant and the regression analysis highlighted the association of risk genotype-interactions with increased THBS1 levels in hypertension. Genetic studies of additional thrombospondin pathway-related genes suggest the complex role of THBS1 in the presence of its family members and the related receptor molecules at HA.


Geosciences ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 512
Author(s):  
Niki Evelpidou ◽  
Anna Karkani ◽  
Maria Tzouxanioti ◽  
Evangelos Spyrou ◽  
Alexandros Petropoulos ◽  
...  

The Cycladic landscape is characterized by landforms of natural beauty and rarity. Landforms resulting from differential erosion, weathering, tectonics, drainage network, sea level changes, and depositional processes can contribute to the development of geotourism in the area. This can be achieved by supporting conservation, protection and promotion of the geo-environment and nature, educating students, residents, and visitors. The aim of this work is to develop an inventory of the main geomorphosites of Paros and Naxos islands by assessing their scientific and additional values, using qualitative and quantitative criteria. Our results show that, besides the high scientific interest of the 75 geomorphosites, most are also characterized by a high ecological value and can potentially lead to a significant increase in the islands’ tourism. The results of this work aim at raise awareness on the geomorphological heritage of central Cyclades and provide a basis for their promotion, protection, and management.


Author(s):  
Eugen Claudiu Jurco ◽  
Grigore Onaciu ◽  
Zamfir Marchis

The study focussed on the Romanian spotted breed-Simmental type was aimed to evaluating the productive potential and some reproductive indices in the period 2013-2015 in Sona farm from Brasov county. The main indicators taken into consideration to analyze the whole herd were the following: milk yield, milk quality, age of first calving, mammary repose and calving interval. Analyzing milk production, this was increasing from 5439 kg in 2013 to 7400 kg in 2015, with an average for the entire period of 6324.33 kg with 4.23% fat and 3.42% protein. Also the fat and protein content have increased over this period, from 4.13 % for fat and 3.25 % for protein in 2013, to 4.34 % and 3.56 respectively in 2015. Analyzing the main indicators of reproduction for a period of three years it was found that calving interval is on average of 401 days and mammary repose is around of 54 days. It is clearly observed that during this period the production is increasing, this shows that cows from the Sona farm records genetic progress regarding qualitative and quantitative milk production. The research has done much to show the productive potential and popularity of the Romanian spotted breed-Simmental type in Transylvania region.


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