Megagametophyte development in Hordeum vulgare. 2. Later stages of wall development and morphological aspects of megagametophyte cell differentiation

1986 ◽  
Vol 64 (10) ◽  
pp. 2327-2336 ◽  
Author(s):  
D. D. Cass ◽  
D. J. Peteya ◽  
B. L. Robertson
Keyword(s):  
Vertical Wall ◽  
Polar Nucleus ◽  
Filiform Apparatus ◽  
Egg Apparatus ◽  
Morphological Aspects ◽  
Partial Fusion ◽  
Membrane Contacts ◽  
Horizontal Wall ◽  
Cell Wall Ingrowths ◽  
Antipodal Cells

The micropylar quartet of nuclei in the barley megagametophyte is first partitioned by a vertical wall between the synergid nuclei and by an initially horizontal wall between the micropylar polar and egg nuclei. The latter wall continues to grow in an expanding horizontal plane forming much of the upper wall of all three egg apparatus cells and eventually fusing with the megagametophyte wall peripherally. A branch of the egg – polar nucleus wall grows in a micropylar direction and becomes attached to the megagametophyte wall. After partitioning, the egg apparatus is composed of three flat cells having a ceiling wall and two upright supporting walls, which are fused centrally. The micropylar polar nucleus lies just chalazal to the ceiling wall. Expansion of the egg apparatus results in rounding of all three cells followed by lengthening and thinning of their walls in contact with the central cell. Probable membrane contacts may facilitate sperm transmission after pollination. Partitioning of the chalazal quartet of nuclei exhibits many similarities to that of the egg apparatus but with a different cellular arrangement. Transfer cell wall ingrowths appear in cells at both poles of the megagametophyte. Such ingrowths appear in the two synergid cells, representing the filiform apparatus. They also develop in two of the original three antipodal cells where these cells are in contact with the megagametophyte wall. Either the micropylar or chalazal polar nucleus migrates to a position close to the other polar nucleus. Partial fusion of polar nuclei occurs later.

scholarly journals Natural convection air flow in vertical upright-angled triangular cavities under realistic thermal boundary conditions

2016 ◽  
Vol 20 (5) ◽  
pp. 1407-1420 ◽  
Author(s):  
Jaime Sieres ◽  
Antonio Campo ◽  
José Martínez-Súarez
Keyword(s):  
Natural Convection ◽  
Vertical Wall ◽  
Aperture Angle ◽  
Temperature Fields ◽  
Uniform Heat Flux ◽  
Thermal Boundary Conditions ◽  
Nusselt Numbers ◽  
Horizontal Wall ◽  
Rayleigh Numbers

This paper presents an analytical and numerical computation of laminar natural convection in a collection of vertical upright-angled triangular cavities filled with air. The vertical wall is heated with a uniform heat flux; the inclined wall is cooled with a uniform temperature; while the upper horizontal wall is assumed thermally insulated. The defining aperture angle ? is located at the lower vertex between the vertical and inclined walls. The finite element method is implemented to perform the computational analysis of the conservation equations for three aperture angles ? (= 15?, 30? and 45?) and height-based modified Rayleigh numbers ranging from a low Ra = 0 (pure conduction) to a high 109. Numerical results are reported for the velocity and temperature fields as well as the Nusselt numbers at the heated vertical wall. The numerical computations are also focused on the determination of the value of the maximum or critical temperature along the hot vertical wall and its dependence with the modified Rayleigh number and the aperture angle.

Effects of wall proximity on the airflow in a vertical solar chimney for natural ventilation of dwellings

2020 ◽  
Vol 44 (3) ◽  
pp. 225-250
Author(s):  
Y Quoc Nguyen ◽  
John Craig Wells
Keyword(s):  
Heat Source ◽  
Natural Ventilation ◽  
Vertical Wall ◽  
Air Gap ◽  
Horizontal Plate ◽  
Solar Chimney ◽  
Horizontal Wall ◽  
Induced Flow ◽  
Air Channel ◽  
Wall Proximity

This study investigates performance of a vertical solar chimney, which absorbs solar energy and induces airflow for natural ventilation and cooling of dwellings, under effects of walls neighboring to its air channel. A computational fluid dynamics model was developed to predict induced flow rate and thermal efficiency of a vertical solar chimney with four types of nearby walls: a vertical wall to which the solar chimney was attached, a horizontal plate above the outlet of the air channel, a horizontal plate, and a horizontal wall below the inlet of the air channel. Examined factors included the heat flux in the air channel, the chimney height, the air gap, the distance of the walls, and the location of the heat source in the air channel. The results showed that effects of the wall proximity were modulated by the location of the heat source and the ratio G/ H between the air gap and the chimney height. Particularly, performance of the chimney was enhanced when the heat source was on the opposite side of the vertical wall and when G/ H was large.

Dynamics of laser-induced cavitation bubbles near two perpendicular rigid walls

2018 ◽  
Vol 841 ◽  
pp. 28-49 ◽  
Author(s):  
Emil-Alexandru Brujan ◽  
Tatsuya Noda ◽  
Atsushi Ishigami ◽  
Toshiyuki Ogasawara ◽  
Hiroyuki Takahira
Keyword(s):  
High Speed ◽  
Vertical Wall ◽  
Bubble Surface ◽  
Bubble Collapse ◽  
High Speed Photography ◽  
Maximum Expansion ◽  
Toroidal Bubble ◽  
Horizontal Wall ◽  
The Moment ◽  
Rigid Walls

The behaviour of a laser-induced cavitation bubble near two perpendicular rigid walls and its dependence on the distance between bubble and walls is investigated experimentally. It was shown by means of high-speed photography with $100\,000~\text{frames}~\text{s}^{-1}$ that an inclined jet is formed during bubble collapse and the bubble migrates in the direction of the jet. At a given position of the bubble with respect to the horizontal wall, the inclination of the jet increases with decreasing distance between the bubble and the second, vertical wall. A bubble generated at equal distances from the walls develops a jet that is directed in their bisection. The penetration of the jet into the opposite bubble surface leads to the formation of an asymmetric toroidal bubble that is perpendicular to the jet direction. At a large distance from the rigid walls, the toroidal bubble collapses in the radial direction, eventually disintegrating into tiny microbubbles. When the bubble is in contact with the horizontal wall at its maximum expansion, the toroidal ring collapses in both radial and toroidal directions, starting from the bubble part opposite to the vertical wall, and the bubble achieves a crescent shape at the moment of second collapse. The bubble oscillation is accompanied by a strong migration along the horizontal wall.

Influence of Upstream Conditions and Gravity on Highly Inertial Thin-Film Flow

2008 ◽  
Vol 130 (6) ◽  
Author(s):  
Roger E. Khayat
Keyword(s):  
Thin Film ◽  
Free Surface ◽  
Flow Field ◽  
Film Flow ◽  
Flow Direction ◽  
Vertical Wall ◽  
Galerkin Projection ◽  
Thin Film Flow ◽  
Thin Film Equations ◽  
Horizontal Wall

Steady two-dimensional thin-film flow of a Newtonian fluid is examined in this theoretical study. The influence of exit conditions and gravity is examined in detail. The considered flow is of moderately high inertia. The flow is dictated by the thin-film equations of boundary layer type, which are solved by expanding the flow field in orthonormal modes in the transverse direction and using Galerkin projection method, combined with integration along the flow direction. Three types of exit conditions are investigated, namely, parabolic, semiparabolic, and uniform flow. It is found that the type of exit conditions has a significant effect on the development of the free surface and flow field near the exit. While for the parabolic velocity profile at the exit, the free surface exhibits a local depression, for semiparabolic and uniform velocity profiles, the height of the film increases monotonically with streamwise position. In order to examine the influence of gravity, the flow is studied down a vertical wall as well as over a horizontal wall. The role of gravity is different for the two types of wall orientation. It is found that for the horizontal wall, a hydraulic-jump-like structure is formed and the flow further downstream exhibits a shock. The influence of exit conditions on shock formation is examined in detail.

Drag, deformation and lateral migration of a buoyant drop moving near a wall

2003 ◽  
Vol 476 ◽  
pp. 115-157 ◽  
Author(s):  
JACQUES MAGNAUDET ◽  
SHU TAKAGI ◽  
DOMINIQUE LEGENDRE
Keyword(s):  
Shear Flow ◽  
Drag Force ◽  
Slip Velocity ◽  
Vertical Wall ◽  
Lateral Migration ◽  
Linear Shear ◽  
Horizontal Wall ◽  
And Migration ◽  
Comparable Magnitude

The problem of a drop of arbitrary density and viscosity moving close to a vertical wall under the effect of buoyancy is analysed theoretically. The case where the suspending fluid is at rest far from the drop and that of a linear shear flow are both considered. Effects of inertia and deformation are assumed to be small but of comparable magnitude, so that both of them contribute to the lateral migration of the drop. Expressions for the drag, deformation and migration valid down to separation distances from the wall of a few drop radii are established and discussed. Inertial and deformation-induced corrections to the drag force and slip velocity of a buoyant drop moving in a linear shear flow near a horizontal wall are also derived.

Analysis of Inclined Wall Plume by the k-ε Turbulence Model

1990 ◽  
Vol 57 (2) ◽  
pp. 455-465 ◽  
Author(s):  
Yusuke Fukushima ◽  
Norio Hayakawa
Keyword(s):  
Similarity Solution ◽  
Flow Direction ◽  
Vertical Wall ◽  
Slope Angle ◽  
Similarity Solutions ◽  
Momentum Balance ◽  
Wide Range ◽  
Horizontal Wall ◽  
Wall Plume ◽  
The Mathematical Model

The similarity solution of inclined wall plume is obtained analytically. The mathematical model used herein consists of the continuity equation of flow, the momentum balance equation in the flow direction, the diffusion equation of concentration, the equation of kinetic energy of turbulence and the equation of viscous dissipation rate of turbulence. It is shown that this set of equations has the similarity solution which can be solved numerically for each angle of the inclined wall. This numerical model is applied to the wide range of the slope angle, which includes the vertical wall plume as the special case and the nearly horizontal wall plume. The velocity and concentration profiles of the inclined wall plume are explained well by the similarity solutions.

The ultrastructure and cytochemistry of the egg apparatus of Brassica campestris

1989 ◽  
Vol 67 (1) ◽  
pp. 177-190 ◽  
Author(s):  
M. J. Sumner ◽  
L. Van Caeseele
Keyword(s):  
Plasma Membrane ◽  
Cell Walls ◽  
Brassica Campestris ◽  
Positive Response ◽  
Uv Light ◽  
Egg Cell ◽  
Filiform Apparatus ◽  
Egg Apparatus ◽  
Large Numbers ◽  
Acidic Polysaccharides

The egg apparatus of Brassica campestris L. cv. Candle (canola-rapeseed) is composed of an egg and two synergids juxtaposed at the extreme micropylar end of the megagametophyte with the egg cell displaced in a chalazal direction. The cell walls of the synergids and egg are uniformly PAS and PA–TCH–SP-positive, but contained β-linked glucans only in the micropylar region. The number and development of the cytoplasmic organelles suggested that the egg cell is relatively inactive metabolically while the synergid cells are active. The synergids contain large numbers of dictyosomes with PA–TCH–SP-positive vesicles at the maturing face. These vesicles appear to fuse with the plasma membrane in the region of the filiform apparatus. The filiform apparatuses of the synergids are micropylar finger-like projections that extend into the cytoplasm of the synergid. These are PAS and PA–TCH–SP-positive, fluoresce in uv light when stained with Calcofluor, and show a positive response for acidic polysaccharides when stained with alcian blue. After treatment with cellulase, fluorescence was not observed. The incipient degenerate synergid was intensely stained by cationic dyes 24–36 h after anthesis.

Natural Convection in a Stably Heated Corner Filled With Porous Medium

1985 ◽  
Vol 107 (2) ◽  
pp. 293-298 ◽  
Author(s):  
S. Kimura ◽  
A. Bejan
Keyword(s):  
Porous Medium ◽  
Natural Convection ◽  
Rayleigh Number ◽  
Single Cell ◽  
Numerical Solutions ◽  
Vertical Wall ◽  
Temperature Fields ◽  
Number Range ◽  
Corner Flow ◽  
Horizontal Wall

This is a study of the single-cell natural convection pattern that occurs in a “stably heated” corner in a fluid-saturated porous medium, i.e., in the corner formed between a cold horizontal wall and a hot vertical wall situated above the horizontal wall, or in the corner between a hot horizontal wall and a cold vertical wall situated below the horizontal wall. Numerical simulations show that this type of corner flow is present in porous media heated from the side when a stabilizing vertical temperature gradient is imposed in order to suppress the side-driven convection. Based on numerical solutions and on scale analysis, it is shown that the single cell corner flow becomes increasingly more localized as the Rayleigh number increases. At the same time, the mass flow rate engaged in natural circulation and the conduction-referenced Nusselt number increase. Numerical results for the flow and temperature fields and for the net heat transfer rate are reported in the Darcy-Rayleigh number range 10–6000.

The development of the central cell of Brassica campestris prior to fertilization

1990 ◽  
Vol 68 (12) ◽  
pp. 2553-2563 ◽  
Author(s):  
M. J. Sumner ◽  
L. van Caeseele
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Brassica Campestris ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Lateral Wall ◽  
Polar Nucleus ◽  
Central Cell ◽  
Transfer Cell ◽  
Egg Apparatus

The development of the central cell of Brassica campestris cv. Candle (canola-rapeseed) was examined using techniques of light and electron microscopy and cytochemistry. The mature central cell is devoid of the large central vacuole characteristic of the early cellular stage of megagametophyte development. Prior to anthesis, cell wall projections, of the transfer cell type, develop on the lateral wall of the central cell. These central cell wall projections extend from the midregion of the megagametophyte to the egg apparatus and are immediately adjacent to the starch-containing region of the inner and outer integuments. The cell wall projections are periodic acid – thiocarbohydrazide – silver proteinate positive as are the contents of dictyosome vesicles that appear to contribute to their formation. Mitochondria are associated with the wall projections as is a network of central cell endoplasmic reticulum that extends from the wall projections to the egg apparatus. Microtubules are associated with the migrating chalazal polar nucleus. The two polar nuclei partially fuse prior to double fertilization, united by nuclear bridges and endoplasmic reticulum interconnections. Proplastids are a characteristic feature of the immature cellular megagametophyte. By anthesis, the proplastids of the mature central cell develop into chloroplasts with stacked thylakoids and starch deposits. Microbodies are frequently found associated with lipid bodies, and polysomes with the endoplasmic reticulum of the mature central cell. Key words: Brassica, central cell, megagametophyte, ovule, transfer cell.

scholarly journals Developmental, ultrastructural and cytochemical investigations of the female gametophyte in Sedum rupestre L. (Crassulaceae)

PROTOPLASMA ◽  
2020 ◽  
Author(s):  
Emilia Brzezicka ◽  
Małgorzata Kozieradzka-Kiszkurno
Keyword(s):  
Female Gametophyte ◽  
Current Knowledge ◽  
Embryo Sac ◽  
Main Function ◽  
Filiform Apparatus ◽  
Electron Dense Material ◽  
Reported Data ◽  
Functional Megaspore ◽  
Sedum Rupestre ◽  
Antipodal Cells

AbstractThis article describes the development of female gametophyte in Sedum rupestre L. New embryological information about the processes of megasporogenesis and megagametogenesis provided in this paper expand the current knowledge about the embryology of the studied species. S. rupestre is characterized by monosporic megasporogenesis and the formation of Polygonum–type embryo sac. The process of megasporogenesis is initiated by one megaspore mother cell, resulting in the formation of a triad of cells after meiosis and cytokinesis. The functional megaspore, which is located chalazally, is a mononuclear cell present next to the megaspore in the centre of the triad. Only one of the two non-functional cells of the triad is binucleate, which occur at the micropylar pole. In this paper, we explain the functional ultrastructure of the female gametophytic cells in S. rupestre. Initially, the cytoplasm of the gametophytic cells does not differ from each other; however, during differentiation, the cells reveal different morphologies. The antipodals and the synergids gradually become organelle-rich and metabolically active. The antipodal cells participate in the absorption and transport of nutrients from the nucellar cells towards the megagametophyte. Their ultrastructure shows the presence of plasmodesmata with electron-dense material, which is characteristic of Crassulaceae, and wall ingrowths in the outer walls. The ultrastructure of synergid cells is characterized by the presence of filiform apparatus and cytoplasm with active dictyosomes, abundant profiles of endoplasmic reticulum and numerous vesicles, which agrees with their main function—the secretion of pollen tube attractants. Reported data can be used to resolve the current taxonomic problems within the genus Sedum ser. Rupestria.

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