Chitin in the symbiotic protist Nephromyces

1986 ◽  
Vol 64 (7) ◽  
pp. 1306-1310 ◽  
Author(s):  
Mary Beth Saffo ◽  
Sara Fultz
Keyword(s):  
Spectral Analysis ◽  
Lectin Binding ◽  
Resistant Cell ◽  
Living Cells ◽  
Morphological Data ◽  
Binding Properties ◽  
Phylogenetic Implications ◽  
Infrared Spectral ◽  
Symbiotic Protist

The endosymbiotic marine protist Nephromyces was first classified as a chytridiomycete by Giard in 1888, yet its taxonomic affinities with chytridiomycetes, as well as with other groups traditionally considered fungi, remain in doubt. To supplement the morphological data on which taxonomic inferences have been based, we assayed Nephromyces for chitin, using three methods: chromatographic assays of acid hydrolysates of alkali-resistant cell residues; infrared spectral analysis; and observations of N-acetylglucosamine-specific lectin-binding properties of the surfaces of living cells. All three assays indicate the presence of chitin in Nephromyces. The possible phylogenetic implications of this finding are discussed.

Leguminous glycosidases: Hydrophobic and lectin binding properties

1989 ◽  
Vol 28 (2) ◽  
pp. 355-357 ◽  
Author(s):  
Wolfgang Einhoff ◽  
Harold Rüdiger
Keyword(s):  
Lectin Binding ◽  
Binding Properties

scholarly journals DISTANT INFRARED SPECTRAL ANALYSIS OF INDUSTRIAL HOT GAS EJECTION IN ATMOSPHERE

2015 ◽  
Vol 3 (7) ◽  
pp. 86-90
Author(s):  
R.S. Asatryan ◽  
N.R. Khachatryan ◽  
H.S. Karayan
Keyword(s):  
Spectral Analysis ◽  
Integral Intensity ◽  
Ecological Monitoring ◽  
Combustion Products ◽  
Industrial Firms ◽  
Industrial Plants ◽  
Hot Gas ◽  
Integral Intensity Ratio ◽  
Infrared Spectral ◽  
Hydrocarbon Gas

Passive Infrared (IR) Spectral-radiometry of gases in the atmosphere is extremely important today, when pollution of the environment by natural ejections and those produced by human activity is growing very high. Particularly, spectral analysis of hot gas ejections i.e. combustion products from industrial plants is an essential part of ecological monitoring of the atmosphere. In this paper we present the results of IR spectral analysis of hot gas ejections from industrial plants in the spectral range from 2.5 to 5.5µm, at a distance of 3000m. The obtained with a hydrocarbon gas group, SO2, N2 O, CO and CO2 gases, as well as H2 O vapor. Relative content of ejected gases (to CO-CO2 group) per unit time was evaluated by means of an integral intensity ratio for each gas. Distant IR Spectral analysis of hot gas ejections (both industrial firms, and various vehicles) have huge value, in particular at ecological monitoring of an environment.

Newly established human pancreatic carcinoma cell lines and their lectin binding properties

1993 ◽  
Vol 13 (1) ◽  
pp. 31-41
Author(s):  
Nobuyuki Nishimura ◽  
Seiji Saito ◽  
Yoshiki Kubota ◽  
Nan-yo Moto-o ◽  
Kuniko Taguchi ◽  
...  
Keyword(s):  
Pancreatic Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Lectin Binding ◽  
Binding Properties ◽  
Carcinoma Cell Lines ◽  
Human Pancreatic Carcinoma Cell

Quantifying Target Occupancy of Small Molecules Within Living Cells

2020 ◽  
Vol 89 (1) ◽  
pp. 557-581 ◽  
Author(s):  
M.B. Robers ◽  
R. Friedman-Ohana ◽  
K.V.M. Huber ◽  
L. Kilpatrick ◽  
J.D. Vasta ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Drug Efficacy ◽  
Living Cells ◽  
Drug Binding ◽  
Therapeutic Window ◽  
Target Engagement ◽  
Binding Properties ◽  
Structure Activity ◽  
Sar Analysis ◽  
Kinetics Of

The binding affinity and kinetics of target engagement are fundamental to establishing structure–activity relationships (SARs) for prospective therapeutic agents. Enhancing these binding parameters for operative targets, while minimizing binding to off-target sites, can translate to improved drug efficacy and a widened therapeutic window. Compound activity is typically assessed through modulation of an observed phenotype in cultured cells. Quantifying the corresponding binding properties under common cellular conditions can provide more meaningful interpretation of the cellular SAR analysis. Consequently, methods for assessing drug binding in living cells have advanced and are now integral to medicinal chemistry workflows. In this review, we survey key technological advancements that support quantitative assessments of target occupancy in cultured cells, emphasizing generalizable methodologies able to deliver analytical precision that heretofore required reductionist biochemical approaches.

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