Sur la variabilité de la capacité rhizogène d'explantats racinaires de Cichorium intybus (var. Witloof) cultivés in vitro : influence de la dimension des explantats initiaux et de la durée de conservation des racines au froid

1986 ◽  
Vol 64 (1) ◽  
pp. 242-246 ◽  
Author(s):  
Jacques Vasseur ◽  
René Lefebvre ◽  
Enoch Backoula
Keyword(s):  
Cold Storage ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Culture Media ◽  
Adventitious Root Formation ◽  
Cichorium Intybus ◽  
Root Production ◽  
Root Explants

On Cichorium intybus root explants of different size, it is possible to demonstrate the existence of a relation between the volume/surface ratio and adventitious root formation capacities. With a volume/surface ratio equal to one, the highest number of adventitious roots and percentage of explants able to produce roots have been observed. When this ratio deviates from unity, adventitious root formation declines. Cold storage of chicory roots causes breakdown of fructosans and accumulation of sucrose, glucose, and fructose. At the same time, adventitious root formation on explants cultured in vitro decreases. Inclusion of glucose in culture media increases adventitious root production whatever the duration of chicory root cold storage may have been. Results are discussed and the hypothesis of a regulation of adventitious roots by sucrose and reducing sugars is advanced.

scholarly journals De novo transcriptome analysis provides insights into formation of in vitro adventitious root from leaf explants of Arnebia euchroma

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jyoti Devi ◽  
Ekjot Kaur ◽  
Mohit Kumar Swarnkar ◽  
Vishal Acharya ◽  
Shashi Bhushan
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Leaf Explants ◽  
Adventitious Root Formation ◽  
Induction Phase ◽  
Arnebia Euchroma ◽  
Lateral Organ ◽  
Organ Boundaries

Abstract Background Adventitious root formation is considered a major developmental step during the propagation of difficult to root plants, especially in horticultural crops. Recently, adventitious roots induced through plant tissue culture methods have also been used for production of phytochemicals such as flavonoids, anthocyanins and anthraquinones. It is rather well understood which horticultural species will easily form adventitious roots, but the factors affecting this process at molecular level or regulating the induction process in in vitro conditions are far less known. The present study was conducted to identify transcripts involved in in vitro induction and formation of adventitious roots using Arnebia euchroma leaves at different time points (intact leaf (control), 3 h, 12 h, 24 h, 3 d, 7 d, 10 d and 15 d). A. euchroma is an endangered medicinal Himalayan herb whose root contains red naphthoquinone pigments. These phytoconstituents are widely used as an herbal ingredient in Asian traditional medicine as well as natural colouring agent in food and cosmetics. Results A total of 137.93 to 293.76 million raw reads were generated and assembled to 54,587 transcripts with average length of 1512.27 bps and N50 of 2193 bps, respectively. In addition, 50,107 differentially expressed genes were identified and found to be involved in plant hormone signal transduction, cell wall modification and wound induced mitogen activated protein kinase signalling. The data exhibited dominance of auxin responsive (AUXIN RESPONSE FACTOR8, IAA13, GRETCHEN HAGEN3.1) and sucrose translocation (BETA-31 FRUCTOFURANOSIDASE and MONOSACCHARIDE-SENSING protein1) genes during induction phase. In the initiation phase, the expression of LATERAL ORGAN BOUNDARIES DOMAIN16, EXPANSIN-B15, ENDOGLUCANASE25 and LEUCINE-rich repeat EXTENSION-like proteins was increased. During the expression phase, the same transcripts, with exception of LATERAL ORGAN BOUNDARIES DOMAIN16 were identified. Overall, the transcriptomic analysis revealed a similar patterns of genes, however, their expression level varied in subsequent phases of in vitro adventitious root formation in A. euchroma. Conclusion The results presented here will be helpful in understanding key regulators of in vitro adventitious root development in Arnebia species, which may be deployed in the future for phytochemical production at a commercial scale.

scholarly journals Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler
Keyword(s):  
Butyric Acid ◽  
Root Development ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
The Other ◽  
Control Group ◽  
Stem Cuttings ◽  
Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

Polyamines and adventitious root formation in Vitis vinifera L.

OENO One ◽  
2002 ◽  
Vol 36 (2) ◽  
pp. 97
Author(s):  
Laurence Geny ◽  
R. Dalmasso ◽  
Michel Broquedis
Keyword(s):  
Vitis Vinifera ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Free Polyamines ◽  
Conjugated Polyamines ◽  
Mean Size ◽  
The Mean

<p style="text-align: justify;">The effects of polyamines were examined for growth and polyamine contents in cultings, callus and primary adventitious roots of <em>Vitis vinifera</em> L. Variations in free, conjugated and wall-bound polyamines in cuttings were observed during rhizogenesis. The main polyamines in cuttings were conjugated polyamines while in callus and primary adventitious roots they were free polyamines. Exogenous polyamine addition did not modify the total number of roots per cutting but increased the mean size and number of long roots. Moreover, exogenous polyamines increased polyamine levels in callus and roots, particurlarly wall-bound and conjugated polyamines. The involvement of these classes of polyamines in morphogenic processes is discussed.</p>

Adventitious root formation of in vitro peach shoots is regulated by auxin and ethylene

2017 ◽  
Vol 226 ◽  
pp. 250-260 ◽  
Author(s):  
Sang-Ho Park ◽  
Mohamed Elhiti ◽  
Huaiyu Wang ◽  
Anna Xu ◽  
Dan Brown ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation

scholarly journals In Vitro and ex Vitro Adventitious Root Formation in Asian Jasmine (Trachelospermum asiaticum) II. Physiological Comparisons

1993 ◽  
Vol 118 (6) ◽  
pp. 906-909 ◽  
Author(s):  
R.C. Apter ◽  
F.T. Davies ◽  
E.L. McWilliams
Keyword(s):  
Nutrient Solution ◽  
Adventitious Roots ◽  
Root Formation ◽  
Initial Dosage ◽  
Root Systems ◽  
Adventitious Root Formation ◽  
Ex Vitro ◽  
Significant Difference ◽  
P Absorption

In vitro tissue-cultured (TC) and macropropagated (MACRO) 18-day old adventitious roots of Asian jasmine [Trachelospermum asiaticum (Siebold & Zucc.) Nakai] were compared for their ability to absorb and translocate radiolabelled P from a nutrient solution. Samples were taken at 1, 2, 4, 8, 12, and 24 hours after the initial dosage of the nutrient solution with 7.4 × 10-2 MBq KH 322PO4/liter. TC roots were capable of absorbing P, but at significantly lower levels than MACRO roots. Greater P absorption occurred in MACRO roots within the first hour and continued for the duration of the experiment. However, there was no significant difference in the rate of P translocation from roots to shoots between treatments. Root systems formed in vitro survived acclimation and had developed into well-branched root systems after 13 weeks. Reduced P absorption by TC roots did not limit either P translocation or survivability during and after acclimation.

scholarly journals Anatomy of Adventitious Root Formation in Stem Cuttings of Mussaenda erythrophylla L. `Rosea'

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 634b-634
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Fast Green ◽  
Phloem Parenchyma ◽  
Ornamental Shrubs ◽  
Vascular Connections

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea, and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mm 1H-indole-3-butyric acid (IBA), or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. For analysis by light microscopy, the basal 1 cm of cuttings was embedded in wax and stained with safranin-fast green. Adventitious roots initiated from phloem parenchyma cells and from basal callus in nontreated cuttings. Cuttings treated with 15 mm IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Root primordia were not observed in non-treated cuttings at 10 days. Root primordia that developed in non-treated cuttings lacked clear vascular connections. These results suggest that non-treated cuttings are difficult to root because few primordia are produced.

Genetic analysis of adventitious root formation in vivo and in vitro in a diversity panel of roses

2020 ◽  
Vol 266 ◽  
pp. 109277
Author(s):  
Thi Hong Nhung Nguyen ◽  
Sophie Tänzer ◽  
Jasmin Rudeck ◽  
Traud Winkelmann ◽  
Thomas Debener
Keyword(s):  
Genetic Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Diversity Panel
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