Phenolic metabolites of Ceratocystis minor from laboratory cultures and their effects on transpiration in loblolly pine seedlings

1986 ◽  
Vol 64 (1) ◽  
pp. 151-155 ◽  
Author(s):  
J. D. DeAngelis ◽  
J. D. Hodges ◽  
T. E. Nebeker
Keyword(s):  
Liquid Medium ◽  
Loblolly Pine ◽  
Water Loss ◽  
Significant Inhibition ◽  
Dendroctonus Frontalis ◽  
Malt Extract ◽  
Fungal Culture ◽  
Phenolic Metabolites ◽  
Pine Seedlings ◽  
Ceratocystis Minor

Several low molecular weight phenolic metabolites of Ceratocystis minor (Hedgcock) Hunt, a blue-stain fungus of loblolly pine, were isolated from cultures grown on 2.5% malt-extract liquid medium. The most abundant compounds were 6,8-dihydroxy-3-hydroxymethyl isocoumarin and 6,8-dihydroxy-3-methyl isocoumarin. Two unknown phenolic compounds were also isolated. Solutions of these fungal metabolites stimulated transpiration in loblolly pine seedlings when administered through the cut stem. Rates of water loss were doubled within 24 h after treatment with isocoumarin solutions. Administration of raw or sterile fungal culture (in liquid medium) on the other hand produced a small but significant inhibition of water loss shortly after treatment. Reduction in transpiration after treatment with fungal culture probably is the result of partial tracheid blockage by as yet unidentified fungal products. Effects of metabolites of C. minor on the water relations of loblolly pine are discussed in terms of a possible mutualistic association between C. minor and the southern pine beetle, Dendroctonus frontalis Zimmermann.

Average radial growth rate and chlamydospore production of Ceratocystis minor, Ceratocystis minor var. barrasii, and SJB 122 in culture

1989 ◽  
Vol 67 (12) ◽  
pp. 3498-3505 ◽  
Author(s):  
David S. Goldhammer ◽  
Frederick M. Stephen ◽  
Timothy D. Paine
Keyword(s):  
Growth Rate ◽  
Uric Acid ◽  
Loblolly Pine ◽  
Radial Growth ◽  
Dendroctonus Frontalis ◽  
Radial Growth Rate ◽  
Acid Media ◽  
Low Concentrations ◽  
Pinus Taeda L ◽  
Ceratocystis Minor

Two symbiotic fungi (SJB 122, an unidentified basidiomycete, and Ceratocystis minor (Hedgecock) Hunt variety barrasii Taylor) and one pathogenic phoretic fungus (C. minor (Hedgecock) Hunt variety minor) of the southern pine beetle, Dendroctonus frontalis Zimmermann, were inoculated onto six different concentrations of D. frontalis frass, loblolly pine (Pinus taeda L.) phloem, and uric acid media to observe radial growth rates and chlamydospore production. The average radial growth rate per day of C. minor var. barrasii increased significantly from the control on all three media, but growth was faster at increased concentrations of added phloem compared with the other supplemented media. Significant increases in chlamydospores produced by C. minor var. barrasii from the control occurred only on frass media, with more chlamydospores being produced at higher concentrations. The average radial growth rate per day of SJB 122 fungus increased significantly from the control on only one concentration of phloem and two concentrations of uric acid, but decreased significantly on low concentrations of frass media. SJB 122 chlamydospore production increased with increasing concentration on frass, was not different from the control on phloem, and increased significantly at intermediate concentrations on uric acid. Ceratocystis minor var. minor average radial growth rate per day increased with increasing concentration on both frass and phloem media but on uric acid decreased significantly at higher concentrations, following an initial signficant increase as compared with the controls.

THE FEEDING POTENTIAL OF CORTICEUS GLABER AND CORTICEUS PARALLELUS (COLEOPTERA: TENEBRIONIDAE), FACULTATIVE PREDATORS OF THE SOUTHERN PINE BEETLE, DENDROCTONUS FRONTALIS (COLEOPTERA: SCOLYTIDAE)

1981 ◽  
Vol 113 (9) ◽  
pp. 807-811 ◽  
Author(s):  
Richard A. Goyer ◽  
Michael T. Smith
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Dendroctonus Frontalis ◽  
Southern Pine Beetle ◽  
Southern Pine ◽  
Pine Beetle ◽  
Blue Stain ◽  
Feeding Potential ◽  
Ceratocystis Minor ◽  
Coleoptera Tenebrionidae

AbstractUnder laboratory conditions, Corticeus glaber (LeConte) and C. parallelus (Melsheimer) adults were facultative predators of southern pine beetle, Dendroctonus frontalis Zimmermann, eggs and first and second instar larvae. Southern pine beetle frass and blue stain fungus, Ceratocystis minor (Hedgecock) Hunt were also suitable for survival for both species of Corticeus. Loblolly pine (Pinus taeda L.) phloem was not utilized as a food source by either species. Corticeus spp. larvae were predators of D. frontalis eggs.

Response of loblolly pine to different inoculum doses of Ceratocystis minor, a blue-stain fungus associated with Dendroctonus frontalis

1987 ◽  
Vol 65 (10) ◽  
pp. 2093-2095 ◽  
Author(s):  
T. D. Paine ◽  
F. M. Stephen
Keyword(s):  
Loblolly Pine ◽  
Dendroctonus Frontalis ◽  
Southern Pine Beetle ◽  
Inducible Defense ◽  
Defense System ◽  
Southern Pine ◽  
Pine Beetle ◽  
Affected Tissue ◽  
Blue Stain ◽  
Ceratocystis Minor

Infection and invasion by the southern pine beetle, Dendroctonus frontalis Zimm., and its associated fungi stimulate the inducible defense system of loblolly pine and result in production of a hypersensitive-like lesion around the affected tissue. The length of the lesion stimulated by inoculation is not related to the amount of inoculum introduced into the tree, even with an eightfold difference. The extent of the response is greater with large inoculum doses, but that is likely to be a function of initial inoculation wound size.

Influence of tree age and growth rate on the radial resin duct system in loblolly pine (Pinus taeda)

1986 ◽  
Vol 64 (5) ◽  
pp. 1046-1049 ◽  
Author(s):  
J. D. DeAngelis ◽  
T. E. Nebeker ◽  
J. D. Hodges
Keyword(s):  
Growth Rate ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Dendroctonus Frontalis ◽  
Age And Growth ◽  
Tree Age ◽  
Secretory Cells ◽  
Tangential Plane ◽  
Cross Sectional ◽  
Resin Duct

Formation of radial resin ducts and their associated secretory cells in loblolly pine (Pinus taeda L.) is influenced by the age and growth rate of the annual ring in which the ducts are formed. The spatial pattern of radial ducts on the tangential plane is nonrandom, exhibiting a regular or dispersed pattern. A significantly higher density of radial ducts was found in the inner, first-formed growth rings at all heights within the tree. Radial duct formation was found to be positively correlated with radial growth rate, when growth rate is expressed as increment of cross-sectional area growth. These findings may partially explain why older, slower growing trees tend to be more susceptible to attack by the southern pine beetle, Dendroctonus frontalis Zimmermann, and associated microorganisms, since the resin-producing system is a primary defense against these agents.

RESOURCE UTILIZATION BY THE SOUTHERN PINE BEETLE, DENDROCTONUS FRONTALIS (COLEOPTERA: SCOLYTIDAE)

1976 ◽  
Vol 108 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Robert N. Coulson ◽  
Adil M. Mayyasi ◽  
J. L. Foltz ◽  
F. P. Hain ◽  
W. C. Martin
Keyword(s):  
Pinus Taeda ◽  
Resource Utilization ◽  
Loblolly Pine ◽  
Quantitative Relationship ◽  
Dendroctonus Frontalis ◽  
Adult Density ◽  
Pine Beetle ◽  
Basal Portion ◽  
Relationship Of ◽  
The Relationship

AbstractThe process of resource utilization by Dendroctonus frontalis Zimmerman attacking loblolly pine, Pinus taeda L., was investigated. The quantitative relationship of attacking parent adult D. frontalis as a function of the normalized infested bole height is described by the model y = Ax(1−x)eBx. Greatest attack density occurs at the mid-bole of the tree and tapers toward the top and bottom. Gallery length (and hence eggs)/100 cm2 was independent of attack density. The relationship between gallery length (or eggs) per parent adult and parent adult density is described by the exponential decay curve y = AeBx, indicating that gallery length and egg population density are controlled by a density dependent compensatory feedback process operating instantaneously. Further support for the mechanism was obtained by analyzing the gallery length per parent adult at different locations on the infested bole. The relationship is described by the model y = [AeBx]/[x(1−x)] and indicates that gallery construction and egg population per attacking beetle increase in the upper and basal portion of the bole. The result is a uniform amount of food and space per individual of the developing population.

scholarly journals An Acaromyces Species Associated with Bark Beetles from Southern Pine Has Inhibitory Properties Against Raffaelea lauricola, the Causal Pathogen of Laurel Wilt Disease of Redbay

2019 ◽  
Vol 20 (4) ◽  
pp. 220-228 ◽  
Author(s):  
Rabiu Olatinwo ◽  
Stephen Fraedrich
Keyword(s):  
Secondary Metabolites ◽  
Loblolly Pine ◽  
Spore Germination ◽  
Bark Beetles ◽  
Dna Analysis ◽  
The United States ◽  
Malt Extract ◽  
Laurel Wilt ◽  
Raffaelea Lauricola ◽  
Redbay Ambrosia Beetle

Laurel wilt is a destructive disease of redbay (Persea borbonia) and other species in the laurel family (Lauraceae). It is caused by Raffaelea lauricola, a fungal symbiont of the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae), cointroduced into the United States around 2002. During assessments of fungi associated with bark beetles from loblolly pine, an unknown fungus was isolated that appeared to have broad-spectrum antifungal activities. In this study, we identified the unknown fungus and determined the inhibitory effect of its secondary metabolites on R. lauricola. DNA analysis identified the fungus as Acaromyces ingoldii (GenBank accession no. EU770231). Secondary metabolites produced by the A. ingoldii completely inhibited R. lauricola mycelial growth on potato dextrose agar (PDA) plates preinoculated with A. ingoldii and reduced R. lauricola growth significantly on malt extract agar plates preinoculated with A. ingoldii. R. lauricola isolates inoculated on PDA plates 7 days after A. ingoldii were completely inhibited with no growth or spore germination. Direct evaluation of A. ingoldii crude extract on R. lauricola spores in a multi-well culture plate assay showed inhibition of spore germination at 10% and higher concentrations. Secondary metabolites from A. ingoldii could be potentially useful in managing the future spread of laurel wilt.

SPATIAL AND TEMPORAL PATTERNS OF WITHIN-TREE COLONIZATION BY DENDROCTONUS FRONTALIS (COLEOPTERA: SCOLYTIDAE)

1978 ◽  
Vol 110 (11) ◽  
pp. 1213-1232 ◽  
Author(s):  
W. Scott Fargo ◽  
Robert N. Coulson ◽  
Paul E. Pulley ◽  
Don N. Pope ◽  
Claude L. Kelley
Keyword(s):  
Loblolly Pine ◽  
Nonlinear Function ◽  
Temporal Sequence ◽  
Dendroctonus Frontalis ◽  
Spatial And Temporal Patterns ◽  
Starting Values ◽  
Frequency Histogram ◽  
Modeling Process ◽  
Standing Trees ◽  
Colonization Process

AbstractWithin-tree colonization by Dendroctonus frontalis infesting loblolly pine, Pinus taeda L., was investigated. Two components of the colonization process were studied: the establishment of attacking adults (ATK) and the ensuing construction of egg galleries (GL). Data on the two variables were taken from standing trees beginning at the time of initial attack and continuing for 14 consecutive days.The spatial and temporal sequence of ATK was described for 1.5 m intervals along the infested bole for the duration of the process. A three parameter nonlinear function was used to describe the data. The pattern of attack was also described as an average process for the entire tree using the same model. A frequency histogram encompassing the range in variation for peak ATK from 134 trees was prepared to provide starting values for simulation purposes.The spatial and temporal sequence of GL construction was described using essentially the same approach as employed for ATK. The modeling process was complicated by loss or obscuring of GL from the radiograph by omission errors and foraging by Monochamus spp. and other associates. GL construction was also described as an average function for the entire tree and the rate of GL construction was defined. A frequency histogram of peak GL was prepared from data on 54 trees for use in selecting starting values for simulation purposes.Numerical relationships between ATK and GL were defined by combining the data on ATK and cumulative expected GL.

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