Probing secondary metabolism in plant cell cultures

1984 ◽  
Vol 62 (12) ◽  
pp. 2912-2917 ◽  
Author(s):  
B. E. Ellis
Keyword(s):  
Secondary Metabolism ◽  
Cell Cultures ◽  
Genome Organization ◽  
Plant Cells ◽  
Cell Manipulation ◽  
Experimental Systems ◽  
Metabolic Integration ◽  
Cell To Cell Variability ◽  
Insight Into

In vitro cultured plant cells which still express parts of their secondary metabolism phenotype are versatile experimental systems for studies of biosynthesis and metabolic integration, enzymology, and genome organization. Single cell manipulation can also provide insight into the question of cell-to-cell variability and metabolic competence.

scholarly journals Natural Variation in Plant Pluripotency and Regeneration

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1261
Author(s):  
Robin Lardon ◽  
Danny Geelen
Keyword(s):  
De Novo ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Relative Role ◽  
Root Explants ◽  
Experimental Systems ◽  
Complex Process ◽  
Insight Into ◽  
Molecular Framework

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

scholarly journals Growing optimization of suspension cultures of medicinal plant cells

2021 ◽  
Vol 291 ◽  
pp. 02022
Author(s):  
Andrey Kolomietc ◽  
Nadezda Nicolaeva ◽  
Victoria Larina ◽  
Nataliya Chupakhina
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Cell Cultures ◽  
Plant Material ◽  
Plant Cells ◽  
Biologically Active ◽  
Suspension Cell ◽  
Active Metabolites ◽  
Isolated Cells

Suspension cell cultures allow to save plant material when obtaining biologically active compounds of natural origin. As a result of the studies, optimal parameters were selected to increase the formation of biologically active metabolites in suspension cell cultures of such medicinal plants as Maackia amurensis Rupr., Hyssopus officinalis L. and Saposhnikovia divaricata (Turcz.) Schischk. Medicinal plants are a large group of plants used as raw materials for the production of medicinal and preventive drugs for medical and animal use. The assortment of phytopreparations is constantly expanding due to the increased demand for natural remedies, due to their less aggressive and toxic nature compared to synthetic ones [1]. Cultivation of medicinal plants in the form of isolated cells in vitro is one of the most modern technologies for rapidly obtaining a large biomass of plant material with stable growth features year-round under controlled conditions [2]. It is known that cells in vitro grow faster and have peculiarities of synthesis and accumulation of biologically active substances compared to intact plants [3]. Isolated cells, unlike tissue cells, also have an advantage for their use as a source of active metabolites, since they have the ability to release these compounds into the intercellular space [4]. The goal of this paper was to select parameters for increasing the biosynthetic activity of cultured suspension cultures of medicinal plant cells in vitro by optimizing cultivation conditions and introducing precursors of secondary metabolite biosynthesis into the nutrient media.

scholarly journals In vitro plant tissue cultures accumulate polyisoprenoid alcohols.

2007 ◽  
Vol 54 (4) ◽  
pp. 847-852 ◽  
Author(s):  
Karolina Skorupinska-Tudek ◽  
Anna Pytelewska ◽  
Monika Zelman-Femiak ◽  
Jakub Mikoszewski ◽  
Olga Olszowska ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Plant Tissue ◽  
Plant Cells ◽  
Ocimum Sanctum ◽  
Taxus Baccata ◽  
Root Cultures ◽  
Tissue Cultures ◽  
Cultivated Plant ◽  
Plant Tissue Cultures

In vitro cultivated plant cells and tissues were found to synthesize polyisoprenoids. Taxus baccata suspension cell cultures accumulated polyisoprenoids of the same pattern as the parental tissue; methyl jasmonate or chitosan treatment almost doubled their content. All the root cultures studied accumulated dolichols as predominant polyisoprenoids. Roots of Ocimum sanctum grown in vitro accumulated approx. 2.5-fold higher amount of dolichols than the roots of soil-grown plants. Dolichols dominated over polyprenols in all Triticum sp. tissues studied.

Cell and tissue culture: potentials for plant breeding

1981 ◽  
Vol 292 (1062) ◽  
pp. 547-556 ◽  
Keyword(s):  
Plant Breeding ◽  
Animal Cell ◽  
Plant Cells ◽  
Intergeneric Hybrids ◽  
Plant Breeder ◽  
Experimental Systems ◽  
Cell Culture Systems ◽  
Animal Cell Cultures ◽  
Mutant Cells

For the plant breeder, one of the objectives of cell culture systems should be their exploitation for the induction and isolation of mutant cells, which can then be regenerated as mutant plants. While a number of mutations have been recognized in plant cells in vitro , few have had any significance for plant breeding. There are currently a number of constraints to the exploitation of this technology, some of which are related to methodological limitations; these are likely to be overcome, but others, which relate to the nature of the attributes that the plant breeder seeks to modify, are much more intractable. There is scope for exploiting cell cutures as genetic tools, as has already been done with animal cell cultures. In contrast, the culture of organized tissues in the form of meristems or small shoots has begun to be useful a technique for plant breeders, and examples of diverse applications will be discussed. Most exploit the rapid rates of multiplication, and the assured health status of the propagules, that can be attained in culture; there is also the possibility of manipulating the genotype of these tissues. Finally, organ culture, and it is the culture of embryos that is of most interest to the plant breeder in this context, is considered; the value of embryo cultuie as a means of producing novel interspecific and intergeneric hybrids is well recognized. In addition, cultured embryos can be used as experimental systems for studying the biochemistry and molecular biology of storage product synthesis and accumulation.

Cotton (Gossypium hirsutum) seed trichomes expand via diffuse growing mechanism

1995 ◽  
Vol 73 (5) ◽  
pp. 746-757 ◽  
Author(s):  
Suresh C. Tiwari ◽  
Thea A. Wilkins
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Seed ◽  
Morphological Changes ◽  
Plant Cells ◽  
Freeze Substitution ◽  
Diffuse Growth ◽  
Cytological Features ◽  
Freeze Fixation ◽  
Insight Into

The ultrastructure of cotton (Gossypium hirsutum) seed trichomes was investigated to obtain insight into their growth behavior during the phase of rapid cell elongation. A mold and cast method of scanning electron microscopy was used to record the morphological changes in the ovular surface during the initiation and elongation of trichomes. A rapid freeze-fixation and freeze-substitution protocol was used to study the cytological features of trichomes at 2 days after anthesis. At the cytological level, attention was primarily focused on determining whether the seed trichomes display features that are characteristic of other tip-growing plant cells, including organelle zonation, polarized deposition of cell wall, axial orientation of microtubules, and microfilament meshwork at the cell apex. Our results show that cotton seed trichomes do not share any ultrastructural characteristic with other tip-growing plant cells. Rather, they show all the characteristics of cells that undergo diffuse growth. The roles of actin microfilaments and microtubules were also investigated through an in vitro administration of cytochalasin D and colchicine. Although the disruption of actin filaments did not stop trichome growth, disruption of microtubules did prevent polarized cell expansion. Based on these results, cotton seed trichomes are not tip-growing cells but expand via diffuse growth. Key words: cytoskeleton, freeze-fixation, freeze-substitution, Gossypium hirsutum, lint fibers, tip growth, trichomes.

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

The in vitro neonatal rat spinal cord preparation: a new insight into mammalian locomotor mechanisms

2004 ◽  
Vol 190 (5) ◽  
pp. 343-357 ◽  
Author(s):  
F. Clarac ◽  
E. Pearlstein ◽  
J. F. Pflieger ◽  
L. Vinay
Keyword(s):  
Spinal Cord ◽  
Neonatal Rat ◽  
Rat Spinal Cord ◽  
Insight Into

scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

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