The role of lipids in the growth of Frankia isolates

1983 ◽  
Vol 61 (11) ◽  
pp. 2801-2806 ◽  
Author(s):  
A. Quispel ◽  
A. J. P. Burggraaf ◽  
H. Borsje ◽  
T. Tak
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Alkaline Hydrolysis ◽  
Chromatographic Analysis ◽  
Tween 80 ◽  
Alnus Glutinosa ◽  
Root Extract ◽  
Myrica Gale ◽  
Thin Layer Chromatographic Analysis

A comparison is made of the lipid requirements of some Frankia strains. Root-extract lipids from alder were essential for the isolation of Frankia strains from all nodules of Alnus glutinosa, Hippophaë rhamnoides, and in one case of Myrica gale and, at least in the case of A. glutinosa, they cannot be replaced by lecithin, Tween 80, or propionate. Many strains no longer need these lipids during subsequent cultivation. Strains from the spore-negative nodules of A. glutinosa need the lipids even after prolonged cultivation on most media, though they can adapt to media with propionate but without root-extract lipids after several transfers. These adapted strains must be distinguished from occasional revertants which, moreover, have a reduced infectivity. The results of silica-gel column and thin-layer chromatographic analysis combined with alkaline hydrolysis indicate that the active factors in the root-extract lipids are special fatty acids either in free or in bound form.

Production of phytotoxic substances by Alternaria triticina

1979 ◽  
Vol 57 (11) ◽  
pp. 1255-1258 ◽  
Author(s):  
C. S. K. Vijaya Kumar ◽  
A. S. Rao
Keyword(s):  
Thin Layer ◽  
Culture Filtrate ◽  
Chromatographic Analysis ◽  
Root Elongation ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Toxic Metabolites ◽  
Thin Layer Chromatographic Analysis ◽  
Phytotoxic Compound

The culture filtrate of Alternaria triticina Prasada & Prabhu inhibited germination and root elongation of seeds of a resistant variety (HD 1982) and a susceptible variety (Kalyan Sona) of wheat. The filtrate-induced symptoms on the leaves of wheat plants were similar to those induced by the fungus. Thin-layer chromatographic analysis of the crude toxin extracted in n-butanol, acetone, and petroleum ether revealed the presence of more than one phytotoxic compound in the culture filtrate of A. triticina. The toxic principles were thermostable and nonspecific and inhibited the growth of wheat. The possible role of toxic metabolites during pathogenesis is suggested.

Intraspecific and interspecific pairing of Haematoloechus medioplexus (Trematoda) and Echinostoma revolutum (Trematoda) adults in vitro, and observations on H. medioplexus lipophilic excretory–secretory products

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 375-380 ◽  
Author(s):  
B. Fried ◽  
D. A. Leiby
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Chromatographic Analysis ◽  
Petri Dish ◽  
Temperature Tolerance ◽  
Echinostoma Revolutum ◽  
Agar Substrate ◽  
Secretory Products ◽  
Thin Layer Chromatographic Analysis

SUMMARYIntraspecific pairing of Haematoloechus medioplexus and Echinostoma revolutum adults and interspecific pairing of H. medioplexus and E. revoltum adults were studied in vitro at 21 ± 1 °C and 30 ± 1 °C in Petri-dish cultures containing an agar substrate and a Locke's 1:1 solution overlay. Salinity and temperature tolerance tests showed that both species survived for 24 h in a Locke's 1:1 solution at 21 ± 1 °C and 30 ±1 °C. For behaviour studies, worms were placed 2 cm apart and then observed at various intervals from 5 min to 4 h. H. medioplexus and E. revolutum showed significant intraspecific pairing at 30±1 °C, whereas only H. medioplexus paired significantly at 21±1°C. Significant interspecific pairing of H. medioplexus and E. revolutum occurred at 21 ±1 °C and 30 ±1 °C. Thin-layer chromatographic analysis showed that H. medioplexus adults released mainly free sterol, along with traces of triacylglycerols and free fatty acids into a non-nutrient medium.

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