Effect of cycloheximide on the freezing tolerance and ultrastructure of cortical parenchyma cells from mulberry twigs

1983 ◽  
Vol 61 (8) ◽  
pp. 2205-2211 ◽  
Author(s):  
T. Niki ◽  
A. Sakai
Keyword(s):  
Freezing Tolerance ◽  
Ultrastructural Changes ◽  
Cortical Cells ◽  
Cytological Change ◽  
Cortical Parenchyma ◽  
Parenchyma Cells ◽  
Cytological Changes ◽  
The Relationship ◽  
Further Development ◽  
In Cells

Experiments were performed using cycloheximide (CHI) on cortical parenchyma cells from mulberry twigs (Morus bombyciz cv. Gorogi) to understand the relationship between the sequence of cytological changes suggesting replacement of the plasmamembrane and the rapid increase in freezing tolerance. Cortical cells from twigs collected on October 16 and 27, which were hardy to −10 and −15 °C, respectively, were still alive after cooling to −70 °C if they had been exposed to −3 °C for 10 to 12 days. In these hardened cells, appearance of microvesicles (MVs) in the peripheral cytoplasm and fusion of these MVs with plasmamembrane were characteristically observed. Neither cytological change nor increase of freezing tolerance was observed in cells treated by CHI before their exposure to −3 °C for 10 to 12 days. However, the suppression of such changes by CHI treatment was effective only in cells which were hardy at −10 to −15 °C and not in the cells of twigs collected on November 4, which were hardy to −20 °C. Consequently, synthesis of some functional proteins during hardening was required for the further development of freezing tolerance, and synthesis had been accomplished before the cells became hardy to −20 °C. Ultrastructural changes brought about by CHI treatment suggest that synthesis of some functional proteins related to the presumed changes of plasmamembrane occurs during hardening.

FORMALIZED DESCRIPTION OF THE PROCEDURE OF INFORMATION SYSTEM RISK MANAGEMENT

2018 ◽  
pp. 61-70
Author(s):  
Svetlana Sergeevna Kozunova ◽  
Alla Grigorievna Kravets
Keyword(s):  
Information System ◽  
Risk Management ◽  
Negative Consequences ◽  
Management Procedure ◽  
System Risk ◽  
Weak Points ◽  
Formalized Description ◽  
Relationship Of ◽  
The Relationship ◽  
Further Development

The article highlights the aspects of risk management in the information system. According to the analysis of the work of Russian and foreign scientists and world practices in the field of risk management, it is stated that there is a need to improve the effectiveness of risk management of information system and to develop a method for managing the risks of the information system. As a solution to the problem of effective risk management of the information system, there has been proposed a formalized procedure for managing the risks of the information system. The scientific novelty of this solution is the use of decision space and optimization space to reduce risks. This procedure allows to assess the damage, risk and effectiveness of risk management of the information system. The risks of the information system are determined and analyzed; a pyramidal risk diagram is developed. This diagram allows you to describe the relationship of risks with the components of the information system. The negative consequences to which these risks can lead are given. The analysis of methods and approaches to risk management has been carried out. Based on the results of the analysis, the methods GRAMM, CORAS, GOST R ISO / IEC scored to the maximum. The weak points of these methods and the difficulty of applying these methods in practice are described. The developed formalized risk management procedure to control the risks of information system can be used as management system’s element of the information security quality that complies with the recommendations of GOST R ISO / IEC 27003-2012. The prospect of further development of the research results is the development of management systems of risk of information system.

scholarly journals The Principle of Directive-Compliant Development of the Law and the Contra Legem Limit

2020 ◽  
Vol 16 (4) ◽  
pp. 465-488
Author(s):  
Thomas M.J. Möllers
Keyword(s):  
Case Law ◽  
Third Party ◽  
Broad Term ◽  
Legal Texts ◽  
Classical Methodology ◽  
The Law ◽  
Legal Methodology ◽  
The Relationship ◽  
Further Development ◽  
Domestic Law

AbstractThe Europeanisation of domestic law calls for a classical methodology to ‘update’ the established traditions of the law. The relationship between European directives and national law is difficult, since directives do apply, but European legal texts need to be implemented into national law. Whilst directives are not binding on private individuals, there is no direct third-party effect, but only an ‘indirect effect’. This effect is influenced by the stipulations of the ECJ, but is ultimately determined in accordance with methodical principles of national law. The ECJ uses a broad term of interpretation of the law. In contrast, in German and Austrian legal methodology the wording of a provision defines the dividing line between interpretation and further development of the law. The article reveals how legal scholars and the case-law have gradually shown in recent decades a greater willingness to shift from a narrow, traditional boundary of permissible development of the law to a modern line of case-law regarding the boundary of directive-compliant, permissible development of the law.

scholarly journals Countrywide survey on utilization of medical devices by GPs in Hungary: advantages of the cluster-practice model

2021 ◽  
Vol 22 ◽  
Author(s):  
Katalin Dózsa ◽  
Fruzsina Mezei ◽  
Tamás Tóth ◽  
Ábel Perjés ◽  
Péter Pollner
Keyword(s):  
Medical Devices ◽  
Point Of Care ◽  
Daily Practice ◽  
Evidence Based ◽  
Self Assessment ◽  
Practice Model ◽  
Practice Models ◽  
Integrate State ◽  
The Relationship ◽  
Further Development

Abstract Background: Expectations towards general practitioners (GPs) are continuously increasing to provide a more systematic preventive- and definitive-based care, a wider range of multidisciplinary team-based services and to integrate state-of-the-art digital solutions into daily practice. Aided by development programmes, Hungarian primary care is facing the challenge to fulfil its role as the provider of comprehensive, high quality, patient-centred, preventive care, answering the challenges caused by non-communicable diseases (NCDs). Aim: The article aims to provide an insight into the utilization of simple, digital, medical devices. We show the relationship between the primary health care (PHC) practice models and the used types of devices. We point at further development directions of GP practices regarding the utilization of evidence-based medical technologies and how such devices support the screening and chronic care of patients with NCDs in everyday practice. Methods: Data were collected using an online self-assessment questionnaire from 1800 Hungarian GPs registered in Hungary. Descriptive statistics, Wilcoxon’s test and χ2 test were applied to analyze the ownership and utilization of 32 types of medical devices, characteristics of the GP practices and to highlight the differences between traditional and cluster-based operating model. Findings: Based on the responses from 27.7% of all Hungarian GPs, the medical device infrastructure was found to be limited especially in single GP-practices. Those involved in development projects of GP’s clusters in the last decade reported a wider range and significantly more intensive utilization of evidence-based technologies (average number of devices: 5.42 versus 7.56, P<.001), but even these GPs are not using some of their devices (e.g., various point of care testing devices) due to the lack of financing. In addition, GPs involved in GPs-cluster development model programmes showed significantly greater willingness for sharing relatively expensive, extra workforce-demanding technologies (χ2 = 24.5, P<.001).

Stimulating the Development of "Greening" of Ukrainian Industry

2021 ◽  
pp. 55-67
Author(s):  
Ella Sheludko ◽  
◽  
Mariia Zavgorodnia ◽  
Keyword(s):  
Public Policy ◽  
Large Scale ◽  
Industrial Enterprises ◽  
Valuation Method ◽  
Circular Model ◽  
Analysis And Synthesis ◽  
Logical Method ◽  
Modern Economic ◽  
The Relationship ◽  
Further Development

The object of this study is the further development of eco-innovations for the rise of industry and the economy. Emphasis is placed on the growing relevance of "green" incentives in line with climate challenges, the economical use of natural resources, as well as the need for a systematic vision of environmental issues and the implementation of international requirements. The study is based on the work of foreign scientists, international rankings and world best practices for the introduction of modern economic mechanisms of state incentives for greening the economy, green modernization, the transition to a circular model of the economy. There is a difference in the implementation of environmental policy - some local projects in Ukraine and the European approach - with the assessment of eco-innovation, systemic change, the formation of ecosystems, scaling technological solutions. The main methods used in the study are: methods of system-structural analysis, analysis and synthesis, grouping - for preliminary analysis and selection of appropriate tools in the study of the implementation of eco-innovation in Ukraine and EU countries; index valuation method and method of comparative analysis - used in the analysis of public policy to stimulate the company to "green" growth; abstract-logical method - used to establish the relationship between the need to introduce new instruments of public policy in the environmental sphere with elements of large-scale reform in the context of climate modernization of industry and to form a systematic vision of major achievements in implementing international requirements for eco-modernization of industrial enterprises. The paper analyzes the forms of international assistance that can compensate for the lack of available financial resources for the purposes of green modernization of the economy in conditions of limited financial capabilities of the state, intensification of competition for European and international environmental investments. The obtained result - a set of possible tools to stimulate Ukrainian industry - allows more systematic implementation of "greening" of Ukrainian industry, and their implementation and combination in a specific mechanism will determine the success of an industrial socially-oriented economy.

scholarly journals A CYTOCHEMICAL DESCRIPTION OF THE MULTIPLICATION OF MENGOVIRUS IN L-929 CELLS

1962 ◽  
Vol 12 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Richard M. Franklin
Keyword(s):  
Phase Contrast ◽  
Basic Protein ◽  
Virus Production ◽  
Cytological Change ◽  
Virus Particles ◽  
Contrast Microscopy ◽  
Infected Cells ◽  
Cytological Changes ◽  
Perinuclear Area

A correlation of cytochemical changes with virus production has been studied in L cells infected with Mengovirus. After a latent period of about 2 hours, virus was produced rapidly, reaching maximum titers of up to 12,000 particles per cell in 6 to 8 hours. The earliest cytological change was in the nucleus and consisted of a slight condensation of chromatin. There is no evidence, however, for the multiplication of either the viral RNA or protein in the nucleus. RNA, of high molecular weight, accumulated in the perinuclear area of the cytoplasm and was later found in inclusions. The perinuclear RNA was digestible with RNase and may be located in or on ribosomes. The inclusion RNA was resistant to RNase but could be removed by pepsin or potassium permanganate; it is probably in completed virus particles. Viral antigen was first observed in a perinuclear location and later in the above-mentioned inclusions. Although the viral protein contains appreciable amounts of arginine and lysine, it is not a basic protein of the histone type. Phase-contrast microscopy of living cells clearly demonstrated the role of the inclusions in release of virus from infected cells. A comparison is made between these cytological changes in Mengo-infected cells and those which have been found by other workers in polio-infected cells. There are many very similar changes.

scholarly journals CONCEPTUAL PROVISIONS ON THE DEVELOPMENT AND IMPLEMENTATION OF THE MECHANISM OF THE INSTITUTE OF TRUST FORMATION IN THE FINANCIAL SERVICES MARKET

2018 ◽  
Vol 1 (2(14)) ◽  
pp. 8-15
Author(s):  
Serhiy Mykolaiovych Shkarlet ◽  
Maksym Viktorovych Dubyna ◽  
Olena Serhiivna Zhuk
Keyword(s):  
Financial Services ◽  
State Regulation ◽  
Trust Building ◽  
Mechanism Of Formation ◽  
Target Setting ◽  
Trust Formation ◽  
New Ideas ◽  
Methodological Principles ◽  
The Relationship ◽  
Further Development

Urgency of the research. In order to develop the financial services market, reform in this area needs to be implemented and measures should be taken to increase the level of trust among the key players in such a market. Target setting. The realities of Ukrainian system of state regulation of the financial intermediaries’ activity testify that the introduction of new ideas, concepts or provisions in this area requires the construction of an effective mechanism for their implementation. It should be clearly defined in the theoretical and methodological plane with the justification of its constituents. Actual scientific research and issues analysis. The works in the field of functioning of the financial services market should include the papers of such scientists: Bobrov Ye. A., Bondarenko Ye. P., Vyshyvana B. M., Voytenko O. M., Kovalenko Yu. M., Rekunenko І. І., Smagin V. L., Unets-Khodakivska V. P., Yuriya S. I., Yashchuk V. V., Asmundson I., Herring R., Stijn C., Wyman О. Uninvestigated parts of general matters defining. Despite the developments in this area, the theoretical and methodological principles of justification of the mechanism of forming confidence in the financial services market are not developed. The research objective. The purpose of the work is to develop the provisions for the creation and implementation of a mechanism for the establishment of a trust institution in the financial services market. The statement of basic materials. The importance of developing and implementing a mechanism of trust building in the financial services market for the further development of such a market is determined. The main elements of such a mechanism, the relationship between them are described and their essence and role in the process of building such a market are determined. Within the mechanism, its purpose, principles, functions, security units, methods and tools are identified. It is indicated that the mechanism should act in order to obtain concrete results, the types of which are defined. Conclusions. The article substantiates the principles of developing and implementing the mechanism of formation of the trust institution in the financial services market.

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