Variegation in Selaginella martensii f. albovariegata. II. Plastid structure in mature leaves

1982 ◽  
Vol 60 (11) ◽  
pp. 2384-2393 ◽  
Author(s):  
James A. Tanno ◽  
Terry R. Webster
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Chloroplast Ultrastructure ◽  
Structural Defects ◽  
Seed Plants ◽  
Wild Type ◽  
Membrane Systems ◽  
High Susceptibility ◽  
Paraffin Sections ◽  
Mature Leaves

Mature green, variegated, and white leaves of Selaginella martensii f. albovariegata were examined by light and electron microscopy. Paraffin sections show the anatomy of all three leaf types to be comparable with that reported for wild type S. martensii. With the light microscope, chloroplasts in green cells appear identical with those of the wild type, while plastids in white cells are smaller and less regular in shape. Chloroplast ultrastructure in green cells is comparable with that described for S. martensii and other members of the genus and quite similar to that typical of seed plants. Plastids of white cells display many structural defects. Large portions of the plastid volume are occupied by vacuoles. In variegated leaves these plastids possess sparse internal membrane systems consisting primarily of irregular vesicles distributed throughout the stroma. In white leaves single lamellae and sets of aligned lamellae are associated with many of the plastid vacuoles. The plastid defects suggest a high susceptibility to photooxidative bleaching.

Development of wheat (Triticum aestivum) pollen wall before and after effect of a gametocide

1990 ◽  
Vol 68 (11) ◽  
pp. 2509-2516 ◽  
Author(s):  
Gamal A. El-Ghazaly ◽  
William A. Jensen
Keyword(s):  
Electron Microscopy ◽  
Triticum Aestivum ◽  
Key Words ◽  
Light And Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Wall ◽  
Seed Plants ◽  
Before And After ◽  
After Effect

Light and electron microscopy studies show that pollen wall development in plants treated with the gametocide RH0007 and untreated plants was similar until the stage at which sporopollenin is normally deposited on the wall. At this stage, the pollen wall of treated plants is 80% thinner than that of the control. Shortly after this stage, the pollen grains in the treated plants collapse and abort. We conclude that the gametocide clearly acts through the inhibition of sporopollenin formation, which results in pollen death. As sporopollenin is found only in the pollen wall of seed plants and the spores of nonseed plants, harm to other parts of the plant is not expected to occur. Key words: pollen wall development, Triticum aestivum, gametocide.

Blistered: a gene required for vein/intervein formation in wings of Drosophila

Development ◽  
1994 ◽  
Vol 120 (9) ◽  
pp. 2661-2671 ◽  
Author(s):  
D. Fristrom ◽  
P. Gotwals ◽  
S. Eaton ◽  
T.B. Kornberg ◽  
M. Sturtevant ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Confocal Microscopy ◽  
Cell Fate ◽  
Light And Electron Microscopy ◽  
Genetic Interactions ◽  
Wing Development ◽  
Wild Type ◽  
Pupal Period ◽  
Vein Formation ◽  
Puparium Formation

We have characterized the blistered (bs) locus phenotypically, genetically and developmentally using a set of new bs alleles. Mutant defects range from wings with ectopic veins and intervein blisters to completely ballooned wings where the distinction between vein and intervein is lost. Mosaic analyses show that severe bs alleles behave largely autonomously; homozygous patches having vein-like properties. Developmental analyses were undertaken using light and electron microscopy of wild-type and bs wings as well as confocal microscopy of phalloidin- and laminin-stained preparations. bs defects were first seen early in the prepupal period with the failure of apposition of dorsal and ventral wing epithelia. Correspondingly, during definitive vein/intervein differentiation in the pupal period (18-36 hours after puparium formation), the extent of dorsal/ventral reapposition is reduced in bs wings. Regions of the wing that fail to become apposed differentiate properties of vein cells; i.e. become constricted apically and acquire a laminin-containing matrix basally. To further understand bs function, we examined genetic interactions between various bs alleles and mutants of two genes whose products have known functions in wing development. (i) rhomboid, a component of the EGF-R signalling pathway, is expressed in vein cells and is required for specification of vein cell fate. rhove mutations (lacking rhomboid in wings) suppress the excess vein formation and associated with bs. Conversely, rho expression in prepupal and pupal bs wings is expanded in the regions of increased vein formation. (ii) The integrin genes, inflated and myospheroid, are expressed in intervein cells and are required for adhesion between the dorsal and ventral wing surfaces. Loss of integrin function results in intervein blisters. Integrin mutants interact with bs mutants to increase the frequency of intervein blisters but do not typically enhance vein defects. Both developmental and genetic analyses suggest that the bs product is required during metamorphosis for the initiation of intervein development and the concomitant inhibition of vein development.

scholarly journals The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

2019 ◽  
Vol 100 (1) ◽  
pp. 161-173 ◽  
Author(s):  
Hideya Kawasaki ◽  
Toshiya Itoh ◽  
Yasuharu Takaku ◽  
Hiroshi Suzuki ◽  
Isao Kosugi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Paraffin Sections

scholarly journals Immunocytochemical localization of hepatic legandin and Z protein utilizing frozen sections for light and electron microscopy.

1979 ◽  
Vol 27 (5) ◽  
pp. 961-966 ◽  
Author(s):  
F Capron ◽  
B Coltoff-Schiller ◽  
A B Johnson ◽  
G M Fleischner ◽  
S Goldfischer
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Light And Electron Microscopy ◽  
Immunocytochemical Localization ◽  
Frozen Sections ◽  
Glutathione S Transferase ◽  
Paraffin Sections ◽  
Organic Ions ◽  
Z Protein

Ligandin (glutathione-s-transferase) and Z protein are soluble hepatocellular proteins that are involved in the transfer of organic ions, including bilirubin and some hormones and carcinogens from the plasma to the liver. The intracellular distribution of ligandin and Z protein was studied by applying the peroxidase-antiperoxidase procedure of L. A. Sternberger (Immunocytochemistry, Prentice Hall Inc., 1974) to paraffin sections and free-floating 10-micrometers frozen sections that were processed for both light and electron microscopy. Ligandin and Z protein were localized to the cytosol of hepatocytes in association with smooth endoplasmic reticulum (SER), but no reaction product was present between cisternae of rough endoplasmic reticulum. Penetration of reagents was enhanced in 10-micrometers frozen sections and the preservation of subcellular structures was equivalent to thicker, unfrozen sections.

scholarly journals Diagnosis of Ion-Exchange Resin Depositions in Paraffin Sections Using Corrective Light and Electron Microscopy-NanoSuit Method

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1193
Author(s):  
Mako Ooishi ◽  
Satoshi Yamada ◽  
Toshiya Itoh ◽  
Shiori Meguro ◽  
Haruna Yagi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Light And Electron Microscopy ◽  
Ion Exchange Resin ◽  
Phosphate Solution ◽  
Ion Exchange Resins ◽  
Paraffin Sections ◽  
Gastrointestinal Complications ◽  
Exchange Resins

Ion-exchange resins are commonly used to treat complications such as hyperkalemia, hyperphosphatemia, and hypercholesterolemia. Gastrointestinal complications may occur as side effects of such treatments. Sodium and calcium polystyrene sulfonate (PS-Ca) are cation-exchange resins comprising an insoluble structure that binds to potassium ions in the digestive tract and exchanges them with sodium and calcium ions, respectively, to promote their elimination. PS crystals are rhomboid, refractive, and basophilic in hematoxylin and eosin staining. To differentiate PS crystals from other ion-exchange resin crystals such as sevelamer and cholestyramine, periodic acid–Schiff, Ziehl–Neelsen, and Congo red staining are usually performed. Here, correlative light and electron microscopy (CLEM)-energy-dispersive X-ray spectroscopy and the NanoSuit method (CENM) was applied to perform a definitive identification of ion-exchange resins. CENM could detect sulfur in PS crystals without destroying the glass slides. Notably, PS retained its ion-exchange ability to bind potassium in paraffin sections. Differential diagnosis of anion-exchange resins, such as sevelamer and cholestyramine, was possible using these characteristics. The phosphorus:carbon ratio was higher in sevelamer than in cholestyramine after soaking paraffin sections in a phosphate solution. Therefore, CENM may be used for the differential pathological diagnosis of ion-exchange resins in paraffin sections.

Tissue section lifting for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 86-87
Author(s):  
Adrian F. van Dellen
Keyword(s):  
Infectious Disease ◽  
Electron Microscopy ◽  
Tissue Culture ◽  
Organ System ◽  
Surrounding Tissue ◽  
Paraffin Sections ◽  
Surface Areas ◽  
Specific Determination ◽  
High Degree ◽  
Accuracy And Repeatability

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

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