Inducing organ generation in vitro: sepal–petal structures from tobacco flower buds

1982 ◽  
Vol 60 (6) ◽  
pp. 845-849 ◽  
Author(s):  
Alan McHughen
Keyword(s):  
Nicotiana Tabacum ◽  
Floral Organ ◽  
Defined Medium ◽  
Variable Number ◽  
Growth Substances ◽  
Flower Buds ◽  
Regulatory Systems ◽  
Nicotiana Tabacum L ◽  
Normal Manner

Tobacco (Nicotiana tabacum L. cv. Wisconsin 38) floral buds with sepal primordia only will continue organ production in a normal manner when excised from the plant and placed on the surface of a simple defined medium lacking growth substances. Such a medium is said to be developmentally inert because it does not induce or direct developmental events in the explant but does permit completion of developmental instructions, if any, carried in the explant. If the medium is supplemented with 10.0 ppm kinetin the bud produces a large but variable number of structures having some characteristics of sepals and some characteristics of petals and then continues with the initiation of fairly normal stamens and then carpels. This paper describes these structures and discusses the effect of kinetin on the regulatory systems controlling floral organ generation.

scholarly journals Antifungal Prenylated Diphenyl Ethers from Arthrinium arundinis, an Endophytic Fungus Isolated from the Leaves of Tobacco (Nicotiana tabacum L.)

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3179 ◽  
Author(s):  
Peng Zhang ◽  
Xin Li ◽  
Xiao-Long Yuan ◽  
Yong-Mei Du ◽  
Bin-Gui Wang ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Line ◽  
Endophytic Fungus ◽  
In Vitro Cytotoxicity ◽  
Ionization Mass ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Nicotiana Tabacum L ◽  
Diphenyl Ethers

An endophytic fungus Arthrinium arundinis TE-3 was isolated and purified from the fresh leaves of cultivated tobacco (Nicotiana tabacum L.). Chemical investigation on this fungal strain afforded three new prenylated diphenyl ethers (1−3) as well as three known analogues (4−6). Structure elucidation of the isolated compounds was carried out by analysis of 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS) spectra, as well as by comparison of those data with literature data. The absolute configuration of the stereogenic center at C-8 in 1 was assigned by comparison of the experimental and calculated ECD spectra. Compounds 1 and 2 showed selective antifungal activity against Mucor hiemalis with minimum inhibitory concentration (MIC) values of 8 and 4 μg/mL, respectively. Compounds 5 and 6 exhibited inhibitory activity against Alteraria alternata with an MIC value of 8 μg/mL. In the cytotoxic assay, 2, 5, and 6 displayed moderate in vitro cytotoxicity against the human monocytic cell line (THP-1 cell line), with IC50 values of 40.2, 28.3, and 25.9 μM, respectively. This study indicated that endophytic fungi possess great potential for exploring new bioactive secondary metabolites.

scholarly journals Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)

2021 ◽  
pp. 39-43
Author(s):  
Ákos Mendel ◽  
László Kovács ◽  
Erzsébet Kiss
Keyword(s):  
Nicotiana Tabacum ◽  
Salt Tolerance ◽  
Lower Class ◽  
Arginine Decarboxylase ◽  
Polyamine Metabolism ◽  
Spermine Synthase ◽  
Nicotiana Tabacum L ◽  
Exogenous Addition ◽  
Precursor Molecules

Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10 mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines.

scholarly journals Growth and Division of Single Cells of Higher Plants in Vitro

1960 ◽  
Vol 43 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Ludwig Bergmann
Keyword(s):  
Nicotiana Tabacum ◽  
Higher Plants ◽  
Single Cells ◽  
Tissue Cultures ◽  
Phaseolus Vulgaris L ◽  
Isolated Cells ◽  
Nicotiana Tabacum L ◽  
Agar Layer ◽  
Petri Dishes

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

In vitro double fertilization in Nicotiana tabacum (L.): the role of cell volume in cell fusion

2001 ◽  
Vol 13 (4) ◽  
pp. 225-229 ◽  
Author(s):  
Meng-Xiang Sun ◽  
Alessandra Moscatelli ◽  
Hong-Yuan Yang ◽  
M. Cresti
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Volume ◽  
Cell Fusion ◽  
Double Fertilization ◽  
Nicotiana Tabacum L

The development of embryo sacs in in vitro ovaries of Nicotiana tabacum L

Plant Science ◽  
1998 ◽  
Vol 132 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Ludmila Lobanova ◽  
Nataliya Enaleeva
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L
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