Cytochemical staining and in vitro activity of acid trimetaphosphatase in etiolated soybean hypocotyls

1981 ◽  
Vol 59 (8) ◽  
pp. 1501-1508
Author(s):  
P. Stôssel ◽  
G. Lazarovits ◽  
E. W. B. Ward
Keyword(s):  
Sodium Fluoride ◽  
Plant Tissues ◽  
Nuclear Staining ◽  
Acid Phosphatases ◽  
In Vitro Activity ◽  
Glutaraldehyde Fixation ◽  
Cytochemical Staining ◽  
Mammalian Tissues ◽  
Cytochemical Marker

Acid trimetaphosphatase has been used as a cytochemical marker for lysosomes in mammalian tissues. Application of the same technique, which employs glutaraldehyde as a fixative, to soybean hypocotyl tissues resulted in staining mainly of nuclei. Sodium fluoride, an effective phosphatase inhibitor, did not prevent but intensified nuclear staining. Nuclei were not stained in formaldehyde-fixed tissues; lead deposits occurred in vacuoles, but ultrastructural preservation was poor and the cytochemical response was inconsistent. Acid trimetaphosphatase activity was demonstrated readily in vitro in protein extracts of soybean tissues, and was strongly inhibited by sodium fluoride. Activity was much reduced in extracts from glutaraldehyde-fixed tissues but not in extracts from formaldehyde-fixed tissues in which it was similar to that in controls. It is concluded that nuclear staining for acid trimetaphosphatase is an artifact of glutaraldehyde fixation, and it is suggested that reports of nuclear staining of acid phosphatases in plant tissues should be interpreted with caution.

Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems

2011 ◽  
Vol 55 (1) ◽  
pp. 178-182 ◽  
Author(s):  
L. A. Tabaldi ◽  
D. Cargnelutti ◽  
G. Y. Castro ◽  
J. F. Goncalves ◽  
R. Rauber ◽  
...  
Keyword(s):  
Vitro Activity ◽  
Acid Phosphatases ◽  
In Vitro Activity

Localization of acid phosphatases in root cap of rice plant

1991 ◽  
Vol 49 ◽  
pp. 224-225
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
W. S. Chen
Keyword(s):  
Rice Plant ◽  
Developmental Stages ◽  
Uranyl Acetate ◽  
Root Cap ◽  
Plant Tissues ◽  
Acid Phosphatases ◽  
Root Tips ◽  
Buffer Solution ◽  
Cacodylate Buffer ◽  
Ethanol Series

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

In Vitro Activity of Phytocannabinoids from High Potency Cannabis sativa

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
A Husni ◽  
S Ross ◽  
O Dale ◽  
C Gemelli ◽  
G Ma ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
High Potency
Sign in / Sign up

Export Citation Format

Share Document