Fusion of protoplasts from carrot cell cultures and the green alga Stigeoclonium

1981 ◽  
Vol 59 (6) ◽  
pp. 1021-1025 ◽  
Author(s):  
Larry C. Fowke ◽  
Harvey J. Marchant ◽  
Peter M. Gresshoff
Keyword(s):  
Polyethylene Glycol ◽  
Green Alga ◽  
Cell Cultures ◽  
Daucus Carota ◽  
Plasma Membranes ◽  
Higher Plant ◽  
Carrot Cell ◽  
Fusion Of Protoplasts ◽  
Fusion Products ◽  
Carrot Cell Cultures

Protoplasts from will carrot (Daucus carota) cell cultures were fused with protoplasts of the filamentous green alga Stigeoclonium sp. using polyethylene glycol. Following fusion of the algal and higher plant plasma membranes, intact Stigeoclonium chloroplasts, nuclei, and centrioles were observed within the carrot cytoplasm. Other algal organelles were not distinguishable in the fusion products.

FUSION OF CARROT AND BARLEY PROTOPLASTS AND DIVISION OF HETEROKARYOCYTES

1976 ◽  
Vol 18 (2) ◽  
pp. 263-269 ◽  
Author(s):  
D. Dudits ◽  
K. N. Kao ◽  
F. Constabel ◽  
O. L. Gamborg
Keyword(s):  
Cell Walls ◽  
Culture Medium ◽  
Cultured Cells ◽  
Differential Staining ◽  
Hordeum Vulgare L ◽  
Daucus Carota L ◽  
Carrot Cell ◽  
Fusion Of Protoplasts ◽  
Fusion Products ◽  
Carrot Cell Cultures

Fusion of protoplasts from cultured cells of carrot (Daucus carota L.) and from leaves of barley (Hordeum vulgare L.) by means of polyethylene glycol resulted in the formation of 4-5 fusion products (heterokaryocytes) per 100 protoplasts. When incubated in culture medium, the heterokaryocytes regenerated cell walls and divided. The frequency of division depended on the viability of the protoplasts from carrot cell cultures, specifically, on the mitotic activity of the cells. Fusion of interphase carrot and barley nuclei was detected by differential staining. Synchronized mitosis was observed in heterokaryons containing barley and carrot nuclei.

The Auxin-like Activity of Humic Substances is Related to Membrane Interactions in Carrot Cell Cultures

2006 ◽  
Vol 33 (1) ◽  
pp. 115-129 ◽  
Author(s):  
A. Muscolo ◽  
M. Sidari ◽  
O. Francioso ◽  
V. Tugnoli ◽  
S. Nardi
Keyword(s):  
Humic Substances ◽  
Cell Cultures ◽  
Membrane Interactions ◽  
Carrot Cell ◽  
Carrot Cell Cultures

Studies of the shear protective effects of Pluronic F-68 on wild carrot cell cultures

2002 ◽  
Vol 12 (3) ◽  
pp. 165-173 ◽  
Author(s):  
D.D Sowana ◽  
D.R.G Williams ◽  
B.K O’Neill ◽  
E.H Dunlop
Keyword(s):  
Cell Cultures ◽  
Protective Effects ◽  
Wild Carrot ◽  
Carrot Cell ◽  
Carrot Cell Cultures

5-Fluorouracil resistance in carrot cell cultures

Planta ◽  
1980 ◽  
Vol 148 (4) ◽  
pp. 389-396 ◽  
Author(s):  
Z. R. Sung ◽  
S. Jacques
Keyword(s):  
Cell Cultures ◽  
Carrot Cell ◽  
Carrot Cell Cultures

Subunit structure and amino acid analyses of β-galactosidase purified from carrot cell cultures

1988 ◽  
Vol 27 (5) ◽  
pp. 1301-1302 ◽  
Author(s):  
Haruyoshi Konno ◽  
Kenji Katoh ◽  
Ichiro Kubota
Keyword(s):  
Amino Acid ◽  
Cell Cultures ◽  
Subunit Structure ◽  
Carrot Cell ◽  
Carrot Cell Cultures

Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors

Planta ◽  
1998 ◽  
Vol 204 (4) ◽  
pp. 490-498 ◽  
Author(s):  
Werner Edgar Gläßgen ◽  
Anette Rose ◽  
Johannes Madlung ◽  
Wolfgang Koch ◽  
Johannes Gleitz ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Cell Cultures ◽  
Anthocyanin Biosynthesis ◽  
Response To Treatment ◽  
Fungal Elicitors ◽  
Carrot Cell ◽  
Carrot Cell Cultures
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