In vitro initiation of adventitious buds and its modification by high concentration of benzyladenine in leaf tissues of mulberry (Morus alba)

1981 ◽  
Vol 59 (1) ◽  
pp. 68-74 ◽  
Author(s):  
S. Oka ◽  
K. Ohyama
Keyword(s):  
Leaf Explants ◽  
Morus Alba ◽  
Adventitious Buds ◽  
High Concentration ◽  
Leaf Tissues ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Bud Initiation ◽  
The Way

Leaf explants of mulberry (Mortis alba L.) derived from aseptically grown shoots and seedlings were cultured on Murashige and Skoog's medium. Normal and abnormal leaves were grown by varying the concentration of benzyladenine. They differed in the way of forming adventitious buds. In normal leaves bud initiation occurred exclusively at the cut ends of midribs after removing petioles, while in abnormal ones buds formed at any region on midribs and petioles. Histological observations were made to study different patterns of bud initiation.

scholarly journals Freesia plantlets from flower-buds cultivated in vitro.

1975 ◽  
Vol 23 (4) ◽  
pp. 334-337
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans
Keyword(s):  
Adventitious Buds ◽  
Flower Buds ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium

Flower-buds of 10 freesia cvs were grown in vitro and were induced to regenerate adventitious buds on a modified Murashige and Skoog's medium containing PBA and IAA. The formation of adventitious buds was strongly enhanced by growing the explants first in darkness and subsequently in light. Subcultured shoots, grown on a medium with IAA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Conditions d'apparition d'une embryogénèse somatique sur des cals issus de la culture de tissus foliaires du chêne vert (Quercus ilex)

1989 ◽  
Vol 67 (4) ◽  
pp. 1066-1070 ◽  
Author(s):  
C. Féraud-Keller ◽  
H. Espagnac
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Quercus Ilex ◽  
Foliar Tissue ◽  
Murashige And Skoog's Medium ◽  
Old Trees ◽  
Murashige And Skoog’S Medium

The proliferation of parenchymatous foliar tissue of Quercus ilex was obtained from current year leaves taken from old trees and cultivated in vitro on modified Murashige and Skoog's medium supplemented with benzylaminopurine (4 mg ∙ L−1) and naphthyl acetic acid (0.5 mg ∙ L−1). Only the fragments cultured in October reacted. The neoformations only appeared on calluses that had not been subcultured for 7 months. Primary nodules arising on these calluses were removed and subcultured on the same medium either in the dark or in the light. In the dark only, they produced secondary nodules, which were the source of somatic embryos both in light and dark. Presently, they seem to regulate their structure in the dark but they do not develop in a way that leads to germination.

scholarly journals Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties

2019 ◽  
pp. 09-15 ◽  
Author(s):  
Pallab Kar ◽  
Arnab Kumar Chakraborty ◽  
Malay Bhattacharya ◽  
Tanmayee Mishra ◽  
Arnab Sen
Keyword(s):  
Special Reference ◽  
Genetic Fidelity ◽  
Fidelity Assessment ◽  
Callus Regeneration ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Phytochemical Studies ◽  
New Crop ◽  
Nodal Culture

Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower which is a good candidate for a new crop for the floriculture industry. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 2 mg/l BAP and 0.5 mg/l

Comportement in vitro de bourgeons axillaires de type indéterminé du palmier dattier (Phoenix dactylifera)

1990 ◽  
Vol 68 (9) ◽  
pp. 2004-2009 ◽  
Author(s):  
Nadia Bouguedoura ◽  
Nicole Michaux-Ferrière ◽  
Jean-Louis Bompar
Keyword(s):  
Floral Induction ◽  
Vegetative State ◽  
Phoenix Dactylifera ◽  
Axillary Buds ◽  
Indolebutyric Acid ◽  
Vegetative Buds ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium

Indeterminate axillary buds excised from young offshoots of date palm (Phoenix dactylifera L.) developed into flowering or vegetative buds when cultured under different in vitro conditions. Floral induction was observed in explants cultured in the dark on Murashige and Skoog's medium supplemented with 50 g∙L−1 of sucrose and several auxins and cytokinins in a ratio favouring the auxins. In contrast, vegetative buds were obtained from explants cultured under a 16-h photoperiod on Murashige and Skoog's medium supplemented with 30 g∙L−1 of sucrose and 1 mg∙L−1 of indolebutyric acid. The results showed that numerous vegetative meristems can be produced from indeterminate buds cultured in vitro. The results also confirmed the observations made during an in vivo study of flowering and vegetative bud development. The importance of the nutritional contribution of the leaves surrounding the flowering buds was pointed out. Key words: Phoenix dactylifera, axillary buds, indeterminate buds, in vitro culture, floral state, vegetative state, morphogenesis.

scholarly journals In vitro Regeneration of Vitex negundo L. - A Multipurpose Woody Aromatic Medicinal Shrub

1970 ◽  
Vol 18 (1) ◽  
pp. 37-42 ◽  
Author(s):  
M. Jawahar ◽  
S. Ravipaul ◽  
M. Jeyaseelan
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Optimal Level ◽  
Vitex Negundo ◽  
Indirect Organogenesis ◽  
Shoot Bud ◽  
Bud Initiation

A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared emerging as green coloured protuberances on the callus. The high frequency of shoot bud initiation and shoot proliferation was observed on MS containing 0.3 mg/l IAA and 0.3 mg/l BAP. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l IBA. Rooted plants were transferred to pots containing sand, soil and manure in the ratio of 1 : 1 : 1. Nearly 90% survival of in vitro plants were recorded. Key words : Vitex negundo, In vitro, Leaf, Callus, Regeneration D.O.I. 10.3329/ptcb.v18i1.3263 Plant Tissue Cult. & Biotech. 18(1): 37-42, 2008 (June)

scholarly journals In Vitro Radical Scavenging and Anti-Yeast Activity of Extracts from Leaves of Aloe Species Growing in Congo

2008 ◽  
Vol 3 (12) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Annalisa Romani ◽  
Pamela Vignolinia ◽  
Laura Isolani ◽  
Sara Tombelli ◽  
Daniela Heimler ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Radical Scavenging ◽  
Hydroxycinnamic Acids ◽  
Quantitative Composition ◽  
High Concentration ◽  
Aloe Barbadensis ◽  
Antimycotic Activity ◽  
Leaf Tissues

Extracts obtained from leaves of Aloe barbadensis and A. congolensis, growing in Congo, were analyzed for their in vitro antiradical and anti-yeast activity. Different leaf tissues (tegument and gel) were analyzed separately. Their phenolic fractions showed the presence of chromones and anthrones (aloesin, aloin B, aloin A, and isoaloeresin), flavonoids (apigenin and kaempferol derivatives), and hydroxycinnamic acids. A differential quantitative composition was observed between leaf tegument and gel: in the first, higher concentrations of the four classes of compounds were observed. The extracts from the tegument exhibited higher in vitro antiradical and antimycotic activity than gel extracts. In a few cases, extracts from teguments were active against amphotericin B-insensitive yeasts. Due to the lack of radical scavenging and yeast inhibition observed when aloin was used, it was possible to postulate that the in vitro activities of the teguments could be related to their high concentration of flavonoids and hydroxycinnamic acids.

scholarly journals Plant Regeneration of Chokecherry (Prunus virginiana L.) from in vitro Leaf Tissues

2004 ◽  
Vol 22 (4) ◽  
pp. 225-228 ◽  
Author(s):  
Wenhao Dai ◽  
Victoria Jacques ◽  
James A. Walla ◽  
Zong-Ming Cheng
Keyword(s):  
Plant Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Regeneration System ◽  
Prunus Virginiana ◽  
Leaf Tissues ◽  
Half Strength ◽  
Eventual Death ◽  
Roots In Vitro

Abstract An effective plant regeneration system was developed for chokecherry (Prunus virginiana L.) by using in vitro leaf tissues. Adventitious shoots regenerated from in vitro leaf tissues only when cultured on Woody Plant Medium (WPM), but not on Murashige and Skoog medium, supplemented with benzyladenine (BA) or thidiazuron (TDZ). Three chokecherry clones (NN, 10, and 17) responded differently to types and concentrations of cytokinins, ranging from 16.7 to 91.7% leaf explants regenerating shoots. A mean of four shoots was produced from each explant, with the most shoots (> 10) from clone NN on media with 5–10 μM BA. Higher concentrations of TDZ (> 8 μM) caused serious vitrification and eventual death of newly induced shoots. Regenerated shoots (> 1.5 cm) produced roots in vitro in half strength MS medium or ex vitro in Cellular Rooting Sponge (CRS) rooting plugs with or without auxin (NAA or IBA) treatments. Rooting was affected by auxin, genotypes, and the rooting methods.

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