The influence of nutrient balance on lignin degradation by the white-rot fungus Phanerochaete chrysosporium

1979 ◽  
Vol 57 (19) ◽  
pp. 2050-2058 ◽  
Author(s):  
Ian D. Reid
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Lignin Degradation ◽  
Nutrient Balance ◽  
White Rot ◽  
Phosphate Limitation ◽  
White Rot Fungus ◽  
Aspen Wood ◽  
Nitrogen Levels ◽  
Carbon:Nitrogen Ratio ◽  
Lignin Metabolism

The degradation by Phanerochaete chrysosporium of "natural" lignin in aspen wood, like synthetic lignin, was inhibited by nitrogen and stimulated by carbohydrate. Nitrogen delayed the appearance and reduced the level of ligninolytic activity and indirectly hastened its decline by accelerating depletion of the carbohydrate supply. The carbon:nitrogen ratio of the medium was a better predictor of lignin degradation than the absolute carbohydrate and nitrogen levels. Unlike nitrogen limitation, sulphate and phosphate limitation of growth did not stimulate lignin metabolism.

Production of H 2 O 2 in Phanerochaete chrysosporium during lignin degradation

1987 ◽  
Vol 321 (1561) ◽  
pp. 455-459 ◽  
Keyword(s):  
Hydrogen Peroxide ◽  
Phanerochaete Chrysosporium ◽  
Essential Role ◽  
Lignin Degradation ◽  
White Rot ◽  
White Rot Fungus ◽  
Glucose Starvation ◽  
Methanol Induction ◽  
Catalysed Oxidation ◽  
Methanol Oxidase

Evidence in support of an essential role for H 2 O 2 in lignin degradation by the white-rot fungus Phanerochaete chrysosporium has been presented by several laboratories. H 2 O 2 is formed simultaneously with the ligninolytic system, and when it is degraded by catalase the lignin-degrading capacity is also reduced. We have now identified, purified and characterized a sugar-oxidizing enzyme that produces H 2 O 2 during glucose starvation in P. chrysosporium . The enzyme oxidizes glucose at the 2-carbon position to yield glucosone, but 5-n-gluconolactone and xylose are also oxidized at significant rates. Another H 2 O 2 -producing enzyme in P.chrysosporium , methanol oxidase, has also been identified, purified and characterized in this laboratory. Methanol is formed from the methoxyl groups in lignin. Hydrogen peroxide, necessary for further degradation of lignin, is formed by enzyme-catalysed oxidation of the lignin-derived methanol. Induction and repression of the H 2 O 2 -producing enzymes is discussed, as well as ways for the fungus to control the glucose level in its environment.

Nutritional regulation of synthetic lignin (DHP) degradation by the selective white-rot fungus Phlebia (Merulius) tremellosa: effects of glucose and other cosubstrates

1991 ◽  
Vol 69 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Ian D. Reid ◽  
Alain M. Deschamps
Keyword(s):  
Energy Source ◽  
Lignin Degradation ◽  
Tricarboxylic Acid Cycle ◽  
Wood Decay ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Biodegradation ◽  
Nutritional Regulation ◽  
Carbohydrate Degradation ◽  
Lignin Metabolism

Phlebia tremellosa is a white-rot fungus which selectively degrades lignin, i.e., its ratio of lignin degradation to carbohydrate degradation during wood decay is higher than that of "simultaneous" white rots. Its need for a cosubstrate to support lignin degradation, and the effect of glucose supply on rate and extent of lignin metabolism, were examined in a synthetic, nitrogen-limited medium. Lignin metabolism by P. tremellosa, like simultaneous white rots, requires a cosubstrate. Glucose partially represses lignin degradation, but it is metabolized to extracellular intermediates, including ethanol. Subsequent utilization of ethanol as energy source supports rapid lignin degradation. Phlebia tremellosa grows well with cellulose, glucose, xylose, ethanol, or lactate as sole carbon (energy) source, and more slowly with glycerol or methanol. It appears unable to use kraft lignin, ferulate, vanillin, or acetate as sole carbon source. Cellulose, glycerol, and ethanol efficiently supported degradation of ring-labelled lignin to CO2, whereas glucose, xylose, and lactate were less efficient cosubstrates; methanol did not support lignin degradation. A relationship between tricarboxylic acid cycle operation and metabolism of lignin ring carbons to CO2, is suggested. Key words: lignin biodegradation, cosubstrate, glucose, ethanol, selectivity.

The Effect of Heavy Metal-Induced Oxidative Stress on the Enzymes in White Rot Fungus Phanerochaete chrysosporium

2014 ◽  
Vol 175 (3) ◽  
pp. 1281-1293 ◽  
Author(s):  
Qihua Zhang ◽  
Guangming Zeng ◽  
Guiqiu Chen ◽  
Min Yan ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

Toxicity of carbon nanotubes to white rot fungus Phanerochaete chrysosporium

2018 ◽  
Vol 162 ◽  
pp. 225-234 ◽  
Author(s):  
Zhu Ming ◽  
Shicheng Feng ◽  
Ailimire Yilihamu ◽  
Shengnan Yang ◽  
Qiang Ma ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Biodegradation of sago effluent by white-rot fungus Phanerochaete chrysosporium

2021 ◽  
Vol 11 (2) ◽  
pp. 91-99
Author(s):  
Vivekanandhan K. ◽  
Dr.M.P. Ayyappadas ◽  
Dr. S. K. Ayyappadas ◽  
R. Renugadevi, ◽  
M. Flory Shobana ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus
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