Lipids in Sphagnum mosses of various ages

1979 ◽  
Vol 57 (12) ◽  
pp. 1335-1339 ◽  
Author(s):  
Pirjo Karunen ◽  
Heikki Mikola ◽  
Reino Linko ◽  
Erkki K. Euranto
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lipid Content ◽  
Dry Weight ◽  
Polar Lipids ◽  
Wax Esters ◽  
Sphagnum Mosses ◽  
Lipid Fractions ◽  
Sphagnum Fuscum ◽  
Layer Chromatography

The lipid content in the annual increments of different ages (1 to 5 years) of Sphagnum fuscum (Schimp.) Klinggr., S. angustifolium (Russow) C. Jens., and S. papillosum Lindb. has been determined by thin-layer chromatography. It was highest in the youngest portion of the moss shoot, ranging from 4.8 to 5.2% of the dry weight and decreased with increasing age to about 40–60% of the original value.The main lipid fractions of the youngest section, the capitulum, of the S. fuscum shoot were steryl and wax esters, triglycerides, and more polar lipids and accounted for 0.4, 0.3, and 3.5%, respectively, of the dry weight. The amounts of these lipids were found to decrease during senescence at independent rates.

Resin production and glandular hairs in Beyeria viscosa (Labill.) Miq. (Euphorbiaceae)

1974 ◽  
Vol 22 (2) ◽  
pp. 195 ◽  
Author(s):  
B Dell ◽  
AJ Mccomb
Keyword(s):  
Endoplasmic Reticulum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Thick Layer ◽  
Dry Weight ◽  
Young Leaf ◽  
Mesophyll Cells ◽  
Glandular Hairs ◽  
Resin Layer ◽  
Layer Chromatography

Leaves of Beyeria viscosa secrete resin with components related to the gibberellins. Two-celled glandular hairs are well developed on the young leaf, and are coated with a thick layer of resin, which makes up almost half the dry weight associated with the young leaf. Plastids of the glandular hairs have poorly developed internal membranes but are enveloped in tubules, apparently derived from endoplasmic reticulum. As the leaf expands, resin secretion ceases; the resin layer is torn apart and is seen largely as caps over the hairs. Resin accounts for some 20% of the dry weight associated with the mature leaf. Resin components also accumulate in the epidermis and certain mesophyll cells. No significant changes take place in the chemistry of the secreted components as the leaves mature, as seen by thin-layer chromatography.

Determination of polar lipids: Quantitative column and thin-layer chromatography

1965 ◽  
Vol 42 (3) ◽  
pp. 215-227 ◽  
Author(s):  
George Rouser ◽  
Gene Kritchevsky ◽  
Claudio Galli ◽  
Dorothy Heller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Polar Lipids ◽  
Layer Chromatography

Determination of α-, β and γ-Amanitin by High Performance Thin-Layer Chromatography in Amanita phalloides (Vaill. ex Fr.) Seer, from Various Origin

1979 ◽  
Vol 34 (12) ◽  
pp. 1133-1138 ◽  
Author(s):  
Tjakko Stijve ◽  
Ruth Seeger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Dry Weight ◽  
Amanita Phalloides ◽  
Prolonged Storage ◽  
Methanolic Extracts ◽  
Layer Chromatography

A fast, sensitive high performance thin-layer chromatographic method for the determination of α-, β-, and γ-amanitin in crude, methanolic extracts of Amanita phalloides is described. The limit of detection is 50 ng of each amanitin. With this method amanitin was determined in 24 pooled samples of Amanita phalloides, collect­ed between 1970 and 1977 in Germany and Switzerland. The total amanitin content varied be­tween 2010 and 7300 mg/kg dry weight and the average value was 4430 mg/kg of which 43% was α-amanitin, 49% β-amanitin and 8% γ-amanitin. The origin of the fungi hardly influenced their amanitin content: in samples collected during the same year at different sites it fluctuated within a factor of 1.7. The amanitin content of samples from the same site, but collected in different years, maximally varied within a factor of 3.7. The partial decomposition of amanitins during prolonged storage of the lyophilized samples undoubtedly contributed to this variation. Phalloidin, which was determined by conventional thin-layer-chromatography, could not be de­tected in a sample from 1970, whereas its concentration in material collected during 1977 amount­ed to 2400 mg/kg dry weight. The toxicity of the samples (LD50 of lyophilized defatted methanolic extracts intravenously for mice) varied within a factor of 2.5.

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