Growth of bean cells in suspension culture in the presence of NaCl and protein-stabilizing factors

1979 ◽  
Vol 57 (7) ◽  
pp. 777-782 ◽  
Author(s):  
J. Gale ◽  
W. G. Boll
Keyword(s):  
Dry Weight ◽  
Cellular Level ◽  
Culture Solution ◽  
Whole Plant ◽  
Significant Stimulation ◽  
Standard Culture ◽  
Culture Growth ◽  
Whole Plants ◽  
Stabilizing Factors ◽  
Cell Culture Growth

Sensitivity of Phaseolus vulgaris to NaCl was much lower in cell suspension than in whole plant cultures. At levels of −4 bars, growth (dry weight accumulation) was decreased by about 40% during the first 9 days. Growth of whole plants was decreased 70% by −2 bars NaCl. There was a small but not statistically significant stimulation of cell culture growth at levels of NaCl between −0.5 and −1.0 bars. (These values are in addition to the −3.5 bars of the standard culture solution itself.)Growth of cells in the control medium stopped at the end of 10 days but continued in the salinized cultures, reaching the level of the controls at about day 13. After six passages in saline medium, there was no evidence of increased tolerance to salt. Sensitivity increased, although subsequent growth in nonsaline medium was not affected.This system was used for assaying the effect of various antioxidants, vitamins, hormones, and amino acids on salt damage at the cellular level. At the concentrations assayed, these materials had either no effect or were toxic.

Effect of NaCl on the Growth of Whole Plants and Their Corresponding Callus Cultures

1981 ◽  
Vol 8 (3) ◽  
pp. 267 ◽  
Author(s):  
MK Smith ◽  
JA Mccomb
Keyword(s):  
Salt Tolerance ◽  
Callus Cultures ◽  
Solution Culture ◽  
Cellular Level ◽  
Plant Responses ◽  
Salt Glands ◽  
Salt Level ◽  
Whole Plant ◽  
Nutrient Solution Culture ◽  
Whole Plants

The effect of NaCl on growth was examined for whole plants and callus cultures of a salt-sensitive glycophyte (Phaseolus vulgaris L.), a salt-tolerant glycophyte (Beta vulgaris L.) and two halophytes (Atriplex undulata D. Dietr., which has salt glands, and Suaeda australis (R. Br.) Moq., a succulent). Whole plants were grown in nutrient solution culture at NaCl concentrations of 0.1-250 mM. Callus cultures were initiated from the same seed stock, and similar saline regimes were imposed. Whole plant responses were characteristic for the various types of plants: P. vulgaris showed a decrease in growth with increasing salinity; B. vulgaris showed a slight increase in growth at the intermediate salt level and a decrease at higher levels; A. undulata and S. australis showed well defined growth optima at 62.5 mM and 125 mM NaCl, respectively. Callus cultures of P. vulgaris and the two halophytes grew very poorly when salinity was increased. Callus of B. vulgaris showed the same tolerance to salt as did the whole plants. Thus salt tolerance of the halophytes depends on the anatomical and physiological complexity of the intact plant while callus from B. vulgaris appears to have a mechanism(s) of salt tolerance which operates at the cellular level.

Effects of kinetin on the growth and development of barley and its interaction with root size

1973 ◽  
Vol 80 (2) ◽  
pp. 211-217 ◽  
Author(s):  
P. Ruckenbauer ◽  
E. J. M. Kirby
Keyword(s):  
Growth And Development ◽  
Dry Weight ◽  
Culture Solution ◽  
Root Tips ◽  
Main Shoot ◽  
Whole Plant ◽  
Cytokinin Synthesis ◽  
Shoot Weight ◽  
Root Apices ◽  
Root Size

SummaryIn some plants the roots have been shown to be major sites of cytokinin production. The part they play in controlling growth and development of cereals was studied by spraying barley with kinetin solution and by changing the size of the root system. Two varieties of barley were grown in culture solution and kinetin was applied and root sexcised at the second-, third- or fifth-leaf stage. The kinetin in aqueous solution was sprayed on the leaves, and either most of the root-tips, or an equivalent weight of entire root strands, were removed. Kinetin increased both shoot apex development and the final ear size. The weights of the main shoot and the whole plant also wore increased, particularly by the later treatments. Removing entire root strands decreased the whole plant dry weight more than removing most of the root apices, but the main shoot weight was less affected. Kinetin interacted with root removal, though neither the interaction nor the main effect of root removal showed unequivocally that cereal roots are the site of cytokinin synthesis, possibly because new root tips were regenerated.

Study of Vidanga on Krimi Roga w.s.r. to Round Worms

2017 ◽  
Vol 2 (04) ◽  
Author(s):  
Abhilasha . ◽  
Vijay Shankar Dubey
Keyword(s):  
Public Health Problem ◽  
Intestinal Wall ◽  
Major Public Health Problem ◽  
Embelia Ribes ◽  
Worm Infestation ◽  
Whole Plant ◽  
The Face ◽  
Whole Plants ◽  
Recurrent Nature ◽  
Weak Resistance

Krimi have been considered a major public health problem through out the world (WHO, 1967 Wandan, 1983 McLaren, 1984). In our country this problem is equally significant. It effects the children more frequently than adult (CCRAS 1987). Krimi hamper growth and development of a person, create malnutritional condition and decrease immunity thus the effective solution of this problem is necessary. Of all the herbs used in treating worm infestation, Vidanga is the best. So, Vidanga was selected for the present study. Whole plant of Embelia ribes Linn. were used as materials, as whole plants are used as medicine. Acharya Charaka describe Krimiroga in Vimanasthana. In most of patients, who are suffering from worm infestation have an Vivarnata (discolouration) on the face. “Vivarnata” had been described by Acharya Charaka under the Lakshana of Purishaja Krimi and it has been also described in Rasavaha Srotodusti Lakshana. Krimis robe the digestive nutritive assets through the intestinal wall, which we are making with high effort. So the deficiency of Rasa Dhatu create. According to Dhatu Poshana Nyaya if the Rasa Dhatu is not sufficient then other Dhatu can’t form properly and ultimate weak resistance and poor immunity develops and it is one cause of recurrent nature of disease. Prakriti Vighata and Nidana Parivarjana eradicate Krimiroga from the base.

scholarly journals Salt-tolerance screening in Limonium sinuatum varieties with different flower colors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaojing Xu ◽  
Yingli Zhou ◽  
Ping Mi ◽  
Baoshan Wang ◽  
Fang Yuan
Keyword(s):  
Salt Tolerance ◽  
Photosynthetic Rate ◽  
Large Scale ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Salt Gland ◽  
Salt Glands ◽  
Chlorophyll Contents ◽  
Relative Water ◽  
Whole Plants

AbstractLimonium sinuatum, a member of Plumbaginaceae commonly known as sea lavender, is widely used as dried flower. Five L. sinuatum varieties with different flower colors (White, Blue, Pink, Yellow, and Purple) are found in saline regions and are widely cultivated in gardens. In the current study, we evaluated the salt tolerance of these varieties under 250 mmol/L NaCl (salt-tolerance threshold) treatment to identify the optimal variety suitable for planting in saline lands. After the measurement of the fresh weight (FW), dry weight (DW), contents of Na+, K+, Ca2+, Cl−, malondialdehyde (MDA), proline, soluble sugars, hydrogen peroxide (H2O2), relative water content, chlorophyll contents, net photosynthetic rate, and osmotic potential of whole plants, the salt-tolerance ability from strongest to weakest is identified as Pink, Yellow, Purple, White, and Blue. Photosynthetic rate was the most reliable and positive indicator of salt tolerance. The density of salt glands showed the greatest increase in Pink under NaCl treatment, indicating that Pink adapts to high-salt levels by enhancing salt gland formation. These results provide a theoretical basis for the large-scale planting of L. sinuatum in saline soils in the future.

scholarly journals Production of cabbage grown in pots containing legumes' root and shoot

Revista CERES ◽  
2012 ◽  
Vol 59 (5) ◽  
pp. 689-694 ◽  
Author(s):  
Thiago de Oliveira Vargas ◽  
Ellen Rúbia Diniz ◽  
Ricardo Henrique Silva Santos ◽  
Alysson Roberto de Almeida ◽  
Segundo Urquiaga ◽  
...  
Keyword(s):  
Block Design ◽  
Mineral Fertilizer ◽  
Dry Weight ◽  
N Fertilization ◽  
Recommended Dose ◽  
Legume Species ◽  
Mineral Fertilization ◽  
Mineral N ◽  
Plant Parts ◽  
Whole Plant

Roots effect is not generally considered in studies assessing the performance of crops in response to green manuring. However, such effect can contribute to a better understanding of crop rotation. The aim of this study was to assess the effect of root and shoot of two legumes on the production of cabbage. The experiment was conducted in pots of 10 liters containing substrate of 2:1 soil/sand. The experiment was arranged in a factorial scheme (2x3 + 2) in a randomized block design with five replicates using two legume species (Crotalaria juncea L. and Canavalia ensiformis L), three plant parts (root, shoot, or whole plant), and two additional treatments (mineral fertilization with 100% and 50% of the recommended dose of N for growing cabbage). Pots with legume treatments received mineral fertilizer with 50% of the recommended dose of N for growing cabbage. The experimental plot consisted of a pot containing one plant of cabbage. Legumes were grown in pots and harvested at 78 days. The root biomass was determined in extra pots. Production was assessed using head fresh and dry weight. The application of the whole plant of both legume species reduced cabbage production. However, root or shoot of both legume species was equivalent to 50% of mineral N fertilization required for the cultivation of cabbage.

scholarly journals ALLELOPATHIC INFLUENCE OF TRIANTHIMA PORTULACASTRUM L. ON GROWTH AND DEVELOPMENTAL RESPONSES OF SESAME (SESAMUM INDICUM L.)

2017 ◽  
Vol 4 (2) ◽  
pp. 183-186
Author(s):  
Prabhakaran J ◽  
Kavitha D
Keyword(s):  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Sesamum Indicum ◽  
Negative Effects ◽  
Chlorophyll Contents ◽  
Whole Plant ◽  
Low Concentrations ◽  
Seedling Length ◽  
Developmental Responses

An experiment was conducted in order to determine the allelopathic effects of the aqueous extract of Trianthima portulacastrum L.on the seed germination, seedling growth and chlorophyll content ofsesame (Sesamum indicum L.). Greenhouse experiment was carried out as RCBD (Randomized complete block design)with four replications. Treatments included 0, 1, 2, 3 and 4% (W/W) residues of whole plant of T.portulacastrum with normal field soil. Results showed that the low concentrations of T.portulacastrum had no significant effect on the germination percentage, seedling length, dry weight, total chlorophyll contents at lower concentration(1%) of weed residues. However, treatments with higher concentrations had negative effects on germination, growth and seedling dry weight of sesame.

scholarly journals Whole-plant Response of Six Poinsettia Cultivars to Three Fertilizer and Two Irrigation Regimes

1996 ◽  
Vol 121 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Ursula Schuch ◽  
Richard A. Redak ◽  
James Bethke
Keyword(s):  
Leaf Area ◽  
Chlorophyll Content ◽  
Dry Weight ◽  
Shoot Biomass ◽  
Vegetative Development ◽  
Chlorophyll Contents ◽  
Shoot Dry Weight ◽  
Irrigation Regimes ◽  
Whole Plant ◽  
Leaf N

Six cultivars of poinsettia (Euphorbia pulcherrima Wind.), `Angelika White', `Celebrate 2', `Freedom Red', `Lilo Red', `Red Sails', and `Supjibi Red' were grown for 9 weeks during vegetative development under three constant-feed fertilizer treatments, 80,160, or 240 mg N/liter and two irrigation regimes, well-watered (high irrigation) or water deficient (low irrigation). Plants fertilized with 80 or 240 mg N/liter were 10% to 18% shorter, while those fertilized with 160 mg N/liter were 25 % shorter with low versus high irrigation. Leaf area and leaf dry weight increased linearly in response to increasing fertilizer concentrations. Low irrigation reduced leaf area, leaf, stem, and shoot dry weight 3670 to 41%. Cultivars responded similarly to irrigation and fertilizer treatments in all components of shoot biomass production and no interactions between the main effects and cultivars occurred. Stomatal conductance and transpiration decreased with increasing fertilizer rates or sometimes with low irrigation. Highest chlorophyll contents occurred in leaves of `Lilo Red' and `Freedom Red'. Leaves of plants fertilized with 80 mg N/liter were deficient in leaf N and had 40 % to 49 % lower leaf chlorophyll content compared to plants fertilized with 160 or 240 mg N/liter. Irrigation had no effect on leaf N or chlorophyll content. At the end of the experiment leaves of `Supjibi Red' and `Angelika White' contained higher concentrations of soluble proteins than the other four cultivars.

scholarly journals Frecuencias de corte y niveles de fertilización nitrogenada en rendimiento y calidad del forrraje de morera (Morus sp.), en Cuyuta, Guatemala

2016 ◽  
Vol 3 ◽  
pp. 48
Author(s):  
Carlos Rodríguez ◽  
Juan A. Quiñones ◽  
Rodrigo Arias
Keyword(s):  
Dry Matter ◽  
Ground Level ◽  
Dry Matter Digestibility ◽  
Yield And Quality ◽  
Whole Plant ◽  
Dual Purpose Cattle ◽  
Whole Plants ◽  
Cutting Height

The trial was conducted at the Centro de Producción Agrícola of ICfA in Cuyuta, Escuintla-Guatemala in order to generate information on the yield and quality of edible mulberry (Morus sp.) roughage treatments consisted of three harvest frequencies (6; 9 and 12 weeks and three fertilization levels of nitrogen (0.40 and 80 kg/ha). A complete randomized block experimental design, with a factorial arrangement (3x3) was used. The cutting height was 0.3 m above the ground level with two sequencies: from August 2nd to September 13 th and from September 13th to December 6th, 1990. In both cases, the 12 week frequency cutting and 80 kg of N were superior to the others (P<=0.01), yielding 6.87 and 6.15 t/ha of dry matter respectively. The yields at 9 weeks were statistically higher than those at 6 weeks. The highest protein percentage of the whole plant, leaves and stalks were produced at 6 weeks. The dry matter digestibility values in vitro showed little variability among treatments, with averages for whole plants, leaves and stalks of 65, 91 and 41 %, respectively. The preceeding data suggests that the mulberry has an excellent potential as a balanced supplement (protein and energy) for dual purpose cattle on the Southern coast of Guatemala.

Climate change: a response surface study of the effects of CO2 and temperature on the growth of beetroot, carrots and onions

1998 ◽  
Vol 131 (2) ◽  
pp. 125-133 ◽  
Author(s):  
D. C. E. WURR ◽  
D. W. HAND ◽  
R. N. EDMONDSON ◽  
J. R. FELLOWS ◽  
M. A. HANNAH ◽  
...  
Keyword(s):  
Climate Change ◽  
Dry Weight ◽  
Fractional Factorial ◽  
Effect Of Temperature ◽  
Maximum Diameter ◽  
Fresh Weight ◽  
Minimum Diameter ◽  
Whole Plant ◽  
Weight Yield ◽  
Erect Leaves

Ten daylit, controlled-environment cabinets were used to investigate the possible impacts of global rises in atmospheric CO2 concentration and temperature on beetroot (Beta vulgaris L.), carrot (Daucus carota L.) and bulb onion (Allium cepa L.) plants. Their responses to CO2 concentrations of 350, 450, 550, 650 and 750 vpm and temperatures of 12, 13·5, 15, 16·5 and 18°C were examined by using a fractional factorial design for the two treatment factors. Use of the daylit cabinets allowed the plants to be grown in natural light, common atmospheric humidities (vpd 0·7 kPa) and non-limiting supplies of water and mineral nutrients.Polynomial models were used to summarize the whole plant dry weight and fresh weight yield responses and to indicate the potential impact of climate change. Additionally, the models were used to generate predictions of the percentage change in whole plant dry weight and plant fresh weight yield for the years 2025 and 2050 relative to 1992. Baseline values of 350 vpm for CO2 and a mean temperature of 13·5°C for 1992 together with forecast CO2 values of 407 and 442 vpm and temperature increases of 0·7 and 1·1 C for 2025 and 2050 respectively were used. For 2025, fresh weight yield changes of +19%, +9% and +13% were obtained for beetroot, carrot and onion crops respectively, while for 2050 the respective changes were +32%, +13% and +21%.Measurements of the ratio of the maximum diameter of the bulb to the minimum diameter of the neck for onions showed that there was little or no influence of CO2, whereas the effect of temperature was substantial. Bulbing was accelerated by high temperature and was greatly delayed at low temperature. At temperatures <15°C, the delays to bulbing resulted in the development of undesirable, thick-necked onions which tended to remain green with erect leaves. These results suggest, therefore, that a warmer climate will be advantageous for the commercial production of bulb onions in Britain.

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