Eutypella parasitica: ascospore germination and mycelial growth

1979 ◽  
Vol 57 (6) ◽  
pp. 624-628
Author(s):  
David W. Johnson ◽  
James E. Kuntz

Ascospore germination of Eutypella parasitica Dav. and Lor. occurred over a temperature range of 12 to 38 °C. Over 90% of the spores which failed to germinate at lower temperatures germinated within 24 h when placed at 24 °C. Percent germination of ascospores varied in the presence of sugar maple and red maple tissue extracts. Germination was best in the presence of outer bark, sapwood, and leaf tissue of sugar maple and red maple and was poorest in the presence of fresh inner bark tissue excised from red maple. Germ-tube development also varied with type of tissue and treatment of tissue. Long, slender, branching germ tubes developed from spores in the presence of nonsterile leaf, bark, and sapwood, whereas short, thick germ tubes developed in the presence of sterilized leaf tissue and sterilized red maple bark. Age of wounded tissue also influenced percent germination of ascospores. Generally germination was higher when pH of these tissues was greater than 5.6. Mycelial growth was best at 29 °C. Growth rate was influenced by type of medium. Both sugar maple and red maple bark incorporated into agar media had a significant inhibitory effect on diameter growth of the fungus when compared with growth on nonamended media.

1974 ◽  
Vol 4 (2) ◽  
pp. 207-212 ◽  
Author(s):  
J. T. Kliejunas ◽  
J. E. Kuntz

Many species of microorganisms were associated with Eutypellaparasitica in cankers on maple. The same species of microorganisms were present in noninoculated artificial branch and trunk wounds on maple trees and in natural wounds on sugar maple and red maple seedlings. There was little antagonistic effect of the associated microorganisms on growth of E. parasitica when paired in culture. Filtrates containing extracts of sugar maple outer bark saprophytes had no effect on spore germination or mycelial growth of E. parasitica. Isolations from branch stubs associated with natural cankers and from tissues in the trunk indicated that E. parasitica invaded tissues colonized first by other microorganisms.


1971 ◽  
Vol 1 (4) ◽  
pp. 228-234 ◽  
Author(s):  
Denis Lachance

Possible ways by which infection with Eutypellaparasitica can occur on sugar and red maple were investigated using various artificial infection courts and different inocula. Close to 50% of wound-inoculated sugar maple trunks became infected, using either mycelium or ascospore inoculum. Red maple was much less susceptible to infection than sugar maple, as only 6% of the tests were positive. Fifteen to 25% of sugar maple branches, 0.25 to 0.5 in. (0.6 to 1.2 cm) in diameter, inoculated with ascospore suspension, became infected when they had received one of the following wounds: knife cut, bruised bark, or incomplete breakage of the branch. No infection occurred when inoculations were attempted at axils of nonwounded 1- to 2-year-old twigs. On many successful trunk inoculations, a slight swelling of the stem and the characteristic mycelial fans of the fungus under the bark were observed 2 years after inoculation. Where rapid extension of the canker occurred, the initial advance of the fungus was in the bark tissue and subsequently in the underlying cambium and xylem. This fungus development corresponds to that observed in naturally infected trees.


2020 ◽  
Vol 52 (3) ◽  
pp. 292-297
Author(s):  
Tara Lee Bal ◽  
Katherine Elizabeth Schneider ◽  
Dana L. Richter

1990 ◽  
Vol 20 (9) ◽  
pp. 1479-1484 ◽  
Author(s):  
F. A. Bazzaz ◽  
J. S. Coleman ◽  
S. R. Morse

We examined how elevated CO2 affected the growth of seven co-occurring tree species: American beech (Fagusgrandifolia Ehrh.), paper birch (Betulapapyrifera Marsh.), black cherry (Prunusserotina Ehrh.), white pine (Pinusstrobus L.), red maple (Acerrubrum L.), sugar maple (Acersaccharum Marsh.), and eastern hemlock (Tsugacanadensis (L.) Carr). We also tested whether the degree of shade tolerance of species and the age of seedlings affected plant responses to enhanced CO2 levels. Seedlings that were at least 1 year old, for all species except beech, were removed while dormant from Harvard Forest, Petersham, Massachusetts. Seeds of red maple and paper birch were obtained from parent trees at Harvard Forest, and seeds of American beech were obtained from a population of beeches in Nova Scotia. Seedlings and transplants were grown in one of four plant growth chambers for 60 d (beech, paper birch, red maple, black cherry) or 100 d (white pine, hemlock, sugar maple) under CO2 levels of 400 or 700 μL•L−1. Plants were then harvested for biomass and growth determinations. The results showed that the biomass of beech, paper birch, black cherry, sugar maple, and hemlock significantly increased in elevated CO2, but the biomass of red maple and white pine only marginally increased in these conditions. Furthermore, there were large differences in the magnitude of growth enhancement by increased levels of CO2 between species, so it seems reasonable to predict that one consequence of rising levels of CO2 may be to increase the competitive ability of some species relative to others. Additionally, the three species exhibiting the largest increase in growth with increased CO2 concentrations were the shade-tolerant species (i.e., beech, sugar maple, and hemlock). Thus, elevated CO2 levels may enhance the growth of relatively shade-tolerant forest trees to a greater extent than growth of shade-intolerant trees, at least under the light and nutrient conditions of this experiment. We found no evidence to suggest that the age of tree seedlings greatly affected their response to elevated CO2 concentrations.


Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Matías Olivera ◽  
Ninoska Delgado ◽  
Fabiola Cádiz ◽  
Natalia Riquelme ◽  
Iván Montenegro ◽  
...  

Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.


1995 ◽  
Vol 12 (3) ◽  
pp. 115-120 ◽  
Author(s):  
David B. Kittredge ◽  
P. Mark S. Ashton

Abstract Browsing preferences by white-tailed deer were evaluated for 6 tree species in northeastern Connecticut. Deer density averaged 23/mile². Deer exhibited no species-specific preferences for seedlings greater than 19 in. For seedlings less than 19 in., hemlock and black birch were preferred. Red maple, sugar maple, and white pine seedlings were avoided. Red oak seedlings were neither preferred nor avoided. A much higher proportion of seedlings greater than 19.7 in. in height was browsed, regardless of species. Browsing preferences for species in the smaller seedling class, combined with a lack of preference for species in the larger class may result in future stands with less diverse tree species composition. Deer densities in excess of 23/mile² may be incompatible with regeneration of diverse forests in southern New England. North. J. Appl. For. 12(3):115-120.


2006 ◽  
Vol 96 (12) ◽  
pp. 1372-1379 ◽  
Author(s):  
Masahiro Kasuya ◽  
Andriantsoa R. Olivier ◽  
Yoko Ota ◽  
Motoaki Tojo ◽  
Hitoshi Honjo ◽  
...  

Suppressive effects of soil amendment with residues of 12 cultivars of Brassica rapa on damping-off of sugar beet were evaluated in soils infested with Rhizoctonia solani. Residues of clover and peanut were tested as noncruciferous controls. The incidence of damping-off was significantly and consistently suppressed in the soils amended with residues of clover, peanut, and B. rapa subsp. rapifera ‘Saori’, but only the volatile substance produced from water-imbibed residue of cv. Saori exhibited a distinct inhibitory effect on mycelial growth of R. solani. Nonetheless, disease suppression in such residue-amended soils was diminished or nullified when antibacterial antibiotics were applied to the soils, suggesting that proliferation of antagonistic bacteria resident to the soils were responsible for disease suppression. When the seed (pericarps) colonized by R. solani in the infested soil without residues were replanted into the soils amended with such residues, damping-off was suppressed in all cases. In contrast, when seed that had been colonized by microorganisms in the soils containing the residues were replanted into the infested soil, damping-off was not suppressed. The evidence indicates that the laimosphere, but not the spermosphere, is the site for the antagonistic microbial interaction, which is the chief principle of soil suppressiveness against Rhizoctonia damping-off.


1987 ◽  
Vol 17 (3) ◽  
pp. 263-266 ◽  
Author(s):  
A. R. C. Jones ◽  
I. Alli

During the spring of 1984 and 1985, white birch (Betulapapyrifera Marsh), sweet birch (B. lenta L), and yellow birch (B. alleghaniensis Britt.) were tapped to determine sap yields and syrup characteristics. These properties were compared with sap yields and syrup produced from sugar maple (Acersaccharum Marsh) and red maple (A. rubrum L). The sap flow seasons were as follows: white birch, 23 days (April 7–29, 1984) and 29 days (April 5 – May 3, 1985); sweet birch, 26 days (1984); yellow birch, 25 days (1985). The sap flow season for the maple species was much earlier than the birch species. Maple sap flow seasons were as follows: sugar maple, 16 days (March 28 – April 12, 1984) and 45 days (March 10 – April 23, 1985); red maple, 44 days (March 11 – April 23, 1985). Sap yields were as follows: white birch, 80.5 L in 1984 (1.0% sap) 51.0 L in 1985 (1.0% sap); sweet birch, 48.0 L in 1984 (0.5% sap); yellow birch, 28.4 L in 1985 (0.5% sap); red maple, 30.6 L in 1985 (2.3% sap); sugar maple, 53.5 L in 1985 (4.5% sap). Sap analyses showed the average total carbohydrate content of all birch saps and all maple saps was 9.2 and 24.5 g/L, respectively. The average sugar contents of the syrups from the birch saps and the maple saps were 302 and 711 g/L, respectively. The average pH of birch and maple saps were similar but the average pH of the syrups obtained from the birch saps was substantially lower than that of the syrups obtained from the maple saps.


1985 ◽  
Vol 15 (5) ◽  
pp. 881-889 ◽  
Author(s):  
Christopher S. Cronan ◽  
Marc R. DesMeules

The Integrated Lake–Watershed Acidification Study (ILWAS) was conducted using three forested watersheds (Panther, Sagamore, and Woods) in the central Adirondack Park of New York State. By comparing the biogeochemical behavior of these watersheds, the ILWAS investigators hoped to elucidate the major ecosystem parameters controlling the fate of strong acids introduced from the atmosphere to lake–watershed systems. The ILWAS vegetation research program was designed to compare the quantitative patterns of forest structure in these midelevation watersheds. Results showed that the ILWAS catchments contain closely related variants of the northern hardwood – spruce – fir complex of the Adirondack region. The dominant tree species in these watersheds are beech (Fagusgrandifolia Ehrh.), red spruce (Picearubens Sarg.), sugar maple (Acersaccharum Marsh.), red maple (Acerrubrum L.), and yellow birch (Betulaalleghaniensis Britt.). On an areal basis, the watersheds contain 57–88% hardwood cover type and 5–29% spruce–fir cover type. Mean live basal area values range from 22 to 30 m2 ha−1 between catchments, while standing dead basal area values range from 4 to 8 m2 ha−1. Mean live stem densities range from 1400 to 1700 stems ha−1. Mean aboveground biomass projections for the tree stratum in the three watersheds range from 143 to 199 Mt ha−1, while estimated aboveground annual biomass increments range from 4.1 to 5.3% of standing biomass.


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