Gametophytes of Lycopodium obscurum as grown in axenic culture

1977 ◽  
Vol 55 (5) ◽  
pp. 563-567 ◽  
Author(s):  
Dean P. Whittier

Gametophytes of Lycopodium obscurum were grown from spores in axenic culture on a medium containing inorganic nutrients and sucrose. The prothalli developed into thick, disk-shaped gametophytes which bore antheridia. No archegonia were observed on these 1-year-old gametophytes. The morphology of the prothalli in culture was essentially that described for prothalli of L. obscurum from nature. However, these Lycopodium gametophytes in culture developed without the endophytic fungus which is necessary for the growth of prothalli of L. obscurum in nature.

1983 ◽  
Vol 61 (9) ◽  
pp. 2369-2373 ◽  
Author(s):  
Dean P. Whittier

Gametophytes of Ophioglossum engelmannii Prantl grow in axenic culture on a nutrient medium containing inorganic nutrients and sucrose. The dark-grown prothalli are long, white, cylindrical structures without rhizoids. The gametophyte has a meristem with a single apical cell. Antheridia, which are partially sunken, and archegonia, which have short necks, are interspersed along the gametophyte. The prothalli are endophyte free. Since the gametophytes of O. engelmannii are undescribed from nature, no comparisons can be made between gametophytes from culture and those from nature. However, gametophytes grown in axenic culture have a morphology which is normal for the genus.


1994 ◽  
Vol 72 (10) ◽  
pp. 1503-1510 ◽  
Author(s):  
K. T. Bird ◽  
J. Jewett-Smith

An in vitro culture system was developed for axenic culture of the seagrass Halophila engelmannii Aschers. The culture system had a rhizome layer solidified with agar (0.8% w/v) and a liquid overlay layer. The rhizome layer contained artificial seawater (20%0, inorganic nutrients, plant growth regulators, sucrose (1% w/v), and activated charcoal (1% w/v). The overlay layer contained artificial seawater and inorganic nutrients. The medium f/4 provided the best source of inorganic nutrients of the five media compared. Axenic cultures of H. engelmannii died if NO3 was the only N source. They grew best when supplied with glutamic acid at a concentration of 3.4 mM as the N source. A combination of α-naphthaleneacetic acid at a concentration of 0.25 mg ∙ L−1 and 6-benzylaminopurine at a concentration of 10 mg ∙ L−1 provided the most rapid rates of propagation as measured by the numbers of pseudowhorls and branches produced. Key words: tissue culture, marine plants, media, plant growth regulators.


2014 ◽  
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Author(s):  
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A. Curir ◽  
R. Drimmel ◽  
M.G. Lattanzi ◽  
P. Re Fiorentin ◽  
...  
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Author(s):  
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C Biasetto ◽  
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N Yokoya ◽  
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Author(s):  
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