An assessment of the modifying effect of kinetin on in vitro tuberization of induced and non-induced tissues of Solatium tuberosum

1976 ◽  
Vol 54 (22) ◽  
pp. 2513-2516 ◽  
Author(s):  
P. L. Forsline ◽  
Alan R. Langille
Keyword(s):  
Culture Medium ◽  
Position Effect ◽  
Percentage Elongation ◽  
Potato Plants ◽  
Solatium Tuberosum ◽  
Modifying Effect ◽  
Katahdin Potato ◽  
Stem Position ◽  
Stem Segments

‘Katahdin’ potato plants (Solatium tuberosum L.) were grown under inducing (26 °C day −12 °C night with an 8-h photoperiod) and non-inducing (28 °C day −25 °C night with a 16-h photoperiod) conditions. Apical, medial, and basal nodal stem segments from each plant harvested were surface sterilized and aseptically transferred to culture flasks containing Murashige and Skoog's medium with and without kinetin. After culturing in dark for 4 weeks at 24 °C, percentage tuberization of segments from induced plants was significantly greater than for non-induced segments. Addition of kinetin to the culture medium eliminated this effect of induction. Apical segments from induced plants tuberized more frequently than those from non-induced plants and those from lower on stem. Addition of kinetin eliminated this stem position effect. Percentage elongation was significantly greater in segments from non-induced than induced plants. Addition of kinetin reduced percentage elongation in non-induced segments to that for induced segments. Percentage elongation was greatest in apical segments from non-induced plants and this position effect could be eliminated by addition of kinetin.

scholarly journals EFFECTS OF AN ANTI-GIBBERELLIN GROWTH RETARDANT ON GROWTH RESPONSES OF POTATO NODAL STEM SEGMENTS CULTURED IN VITRO

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 188d-188
Author(s):  
Berardo Escalante ◽  
Alan R. Langile
Keyword(s):  
Culture Media ◽  
Fold Increase ◽  
Plant Surface ◽  
Growth Responses ◽  
Potato Plants ◽  
Final Treatment ◽  
Nodal Stem Segments ◽  
Katahdin Potato ◽  
Stem Segments

Foliage of non-induced `Katahdin' potato plants was treated with BAS-111. Other plants were sprayed with GA3 solution and placed in an inducing chamber. All treatments were repeated the following week. After final treatment, apical, sub-apical, medial, and basal nodal stem segments were taken from each plant, surface-sterilized, and placed on MS culture media. After 3 weeks in a darkened incubator, cultures were examined. Induced plants produced 5.5 times more tubers than did non-induced segments. BAS-111 applied to non-induced plants was associated with 63% reduction in rhizome length and 3.2-fold increase in tuber number. GA treatment to induce plants resulted in improved rhizome elongation, delayed and reduced tuberization when compared with control explants. Lower nodes produced more and larger tubers than did younger tissues. Results will be discussed in light of current literature.

scholarly journals THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS

2016 ◽  
Vol 24 ◽  
pp. 79-82
Author(s):  
H. M. Shevaha ◽  
M. M. Kyryk ◽  
V. M. Hunchak ◽  
T. M. Oliinyk
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
In Vitro Growth ◽  
Maize Starch ◽  
Potato Plants

Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

scholarly journals Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

2012 ◽  
Vol 40 (1) ◽  
pp. 107 ◽  
Author(s):  
Cuiqin YANG ◽  
Yaoguo QIN ◽  
Xin SUN ◽  
Shu YUAN ◽  
Honghui LIN
Keyword(s):  
Shoot Regeneration ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Leaf Base ◽  
Stem Segments

An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric plantlets after an additional 10 days. The hyperhydric plantlets reversed to normal plantlets when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.

scholarly journals Effect of agar concentration in culture medium on in vitro development of potato plants

2020 ◽  
Vol 9 (7) ◽  
pp. e542974571
Author(s):  
Cinthia Kutz de Matos ◽  
Camila Eduarda Loli Pereira ◽  
Leonardo Balena ◽  
Jackson Kawakami
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Dry Mass ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Semisolid Medium ◽  
Potato Plants ◽  
Best Response ◽  
Vitro Development

In vitro propagation and tissue culture technique is an alternative for obtaining healthy potato seeds. However, data found in the literature differ on the optimal consistency of the culture medium to be used in vitro. The aim in this research was to look at the adaptation of potato cultivars to medium with different agar concentrations. The experimental design was entirely randomized in a factorial scheme. The experiment tested the effect of 3 agar medium concentrations (solid, semisolid and liquid) on 6 cultivars (Agata, Bel, Camila, Catucha, Clara and Eliza), with 5 repetitions. We quantified from the plantlets, fresh and dry mass of roots and shoot, the number of nodes, the multiplication capacity and vigor. The study showed that potato cultivars respond significantly to different concentrations of agar and their best response was in the semisolid medium.

Photoperiod, Temperature, Gibberellin, and an Anti-gibberellin Affect Tuberization of Potato Stem Segments In Vitro

HortScience ◽  
1998 ◽  
Vol 33 (4) ◽  
pp. 701-703 ◽  
Author(s):  
B.Z. Escalante ◽  
Alan R. Langille
Keyword(s):  
Foliar Spray ◽  
Fresh Mass ◽  
Nodal Segments ◽  
Tuber Number ◽  
Distilled Water ◽  
Orthogonal Contrasts ◽  
Potato Plants ◽  
Node Location ◽  
Stem Segments

`Katahdin' potato plants were grown under conditions that did not induce tuberization (noninducing conditions) and the foliage was sprayed with either a growth retardant (BAS-111) at 1000 mg·L-1 or distilled water. Other plants, grown under tuber-inducing conditions, received a foliar spray of gibberellic acid (GA3) at 100 mg·L-1 or distilled water. After 1 week, treatments were repeated. Two-node stem segments were excised from the apical, subapical, medial, and basal sections of each plant 72 hours after the second foliar treatment, disinfested, and inserted into flasks containing 50 mL of Murashige and Skoog medium (2% sucrose). After 3 weeks in a darkened incubator adjusted to 24 °C, tuberization response was evaluated. Orthogonal contrasts revealed significant differences between induced and noninduced controls for tuber number, diameter, and fresh mass. BAS-111 reduced rhizome length and increased tuber number, diameter, and fresh mass. GA3 increased rhizome length, but reduced tuber number, diameter, and fresh mass. Node location influenced tuber development, as basal explants produced significantly more and larger tubers, as well as longer rhizomes, than did apical explants, and subapical segments produced more and larger tubers than did apical segments. There were no significant differences between medial and basal nodal segments with respect to tuber number or tuber/rhizome size. Chemical names used: 1-phenoxy-5,5-dimethyl-3-(1,2,4-triazol-1-yl)-hexan-5-ol (BAS-111); 2,4a,7-trihydroxy-1-methyl-8-methylenegibb-3-ene-1,10-carboxylic acid 1->4 lactone (GA3).

scholarly journals In Vitro Plant Regeneration of Mexican Lime and Mandarin by Direct Organogenesis

HortScience ◽  
1997 ◽  
Vol 32 (5) ◽  
pp. 931-934 ◽  
Author(s):  
Eugenio Pérez-Molphe-Balch ◽  
Neftalí Ochoa-Alejo
Keyword(s):  
Culture Medium ◽  
Adventitious Shoots ◽  
Direct Organogenesis ◽  
Soil Conditions ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Efficient System ◽  
Mexican Lime ◽  
Stem Segments

An efficient system for in vitro regeneration by organogenesis starting from internodal stem segments from seedlings of Mexican lime (Citrus aurantifolia Christm. Swing.) and mandarin (C. reticulata Blanco cv. Monica) was developed. The best results were obtained when the wounded edges of internodal stem segments cut longitudinally were placed downward on the surface of the culture medium. The optimal culture medium from both species was Murashige and Skoog with vitamins from B5 medium, 5% sucrose, 33.3 μm BA and 5.4 μm NAA. The best response was obtained when the segments were incubated at 25 ± 2 °C for 21 d in darkness, followed by 29 d on a 16/8-h light/dark cycle (fluorescent light, 54 μmol·m-2·s-1). The best regeneration system tested allowed the attainment of adventitious shoots from 96% and 88% of the explants in Mexican lime and mandarin, respectively. In Mexican lime an average of 7.8 well-differentiated shoots per explant was obtained, and in mandarin the yield was 5.1. Rooting of 70% of the shoots was achieved in culture medium with NAA (2.7–5.4 μm) or IBA (2.5–4.9 μm). Of the rooted plants, 85% adapted well to soil conditions. Chemical names used: 6-benzylaminopurine (BA), α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA).

scholarly journals Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

2021 ◽  
Vol 38 ◽  
pp. 00139
Author(s):  
Natalia Yegorova ◽  
Irina Stavtzeva ◽  
Victor Zolotilov
Keyword(s):  
Essential Oil ◽  
Embryo Culture ◽  
Interspecific Hybrids ◽  
Culture Medium ◽  
High Variability ◽  
Ms Medium ◽  
Clonal Micropropagation ◽  
Hybrid Seedling ◽  
Stem Segments

The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.

scholarly journals Factors affecting adventitious root formation in isolated stem segments of Rhododendron.

1969 ◽  
Vol 17 (3) ◽  
pp. 203-208
Author(s):  
R.L.M. Pierik
Keyword(s):  
Mineral Nutrition ◽  
Adventitious Root ◽  
Culture Medium ◽  
Root Formation ◽  
Continuous Light ◽  
Adventitious Root Formation ◽  
Continuous Darkness ◽  
Factors Affecting ◽  
Stem Segments

Stem expiants of the rhododendron cultivar Catawbiense Album rooted more easily in vitro than those of Pink Pearl, agreeing with the experience of nursery practice. Rooting occurred only on segments of young soft shoots and was strongly promoted when the expiants were placed inverted on the medium. Continuous light inhibited, whereas continuous darkness promoted rooting. Rooting occurred only in the presence of an auxin together with a sugar in the culture medium. There was no evidence that mineral nutrition and temperatures between 21 degrees and 29 degrees C. play an important role.-Univ. Wageningen. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals In vitro Plant Regeneration from Callus of Citrus x monstruosa (Pompia), an Endemic Citrus of Sardinia

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Daniele Fraternale ◽  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Pierpaolo Cara ◽  
Donata Ricci
Keyword(s):  
Survival Rate ◽  
Culture Medium ◽  
Basal Medium ◽  
In Vitro Plant Regeneration ◽  
Response Frequency ◽  
Half Strength ◽  
Pre Treatment ◽  
Cut Surface ◽  
Stem Segments

A regeneration protocol was developed from callus obtained from various explants taken from in vitro cultured seedlings (root, leaf and stem internodes) of Citrus x monstruosa. The best treatment in terms of response frequency and mean number of shoots for explants was 35.0 μM BA with 5.5 uM NAA. The best shoot regeneration was obtained with internodal stem segments cut longitudinally with the cut surface in contact with the culture medium and pre-treatment of 21 days of these explants in darkness. The best rooting of explants was obtained on half-strength MS basal medium supplemented with either NAA or IBA at 5.4 μM and 2.5 μM, respectively. Hardening of Citrus x monstruosa was accomplished in 40 days, with 95% survival rate.

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

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