Some possible sources of error in determining bulk elastic moduli and other parameters from pressure–volume curves of shoots and leaves

1976 ◽  
Vol 54 (8) ◽  
pp. 758-765 ◽  
Author(s):  
Y. N. S. Cheung ◽  
M. T. Tyree ◽  
J. Dainty

Pressure–volume curves were constructed from well defined models and hypothetical shoots in which reasonable values of osmotic pressure and cell wall elastic moduli were specified for cell types of different relative volumes. The pressure–volume curves so obtained closely resembled those of real shoots and leaves. Comparing the bulk parameters obtained from analysis of the constructed pressure–volume curves with the values defined in the models allows us to examine the sources of error in their evaluation. The graphical values of the original bulk osmotic pressure and of the total volume of osmotic (symplasmic) water agreed very well with those defined; however, the osmotic pressure at incipient plasmolysis and the bulk elastic moduli estimated from the graph were generally lower than their actual values originally used in the models. We show that the apparent linear dependence of the bulk elastic modulus of sitka spruce reported by Hellkvist et al. (1974) may not reflect a similar linearity for the elastic moduli of individual cells.

1985 ◽  
Vol 8 (8) ◽  
pp. 563-570 ◽  
Author(s):  
HSIN-I WU ◽  
RICHARD D. SPENCE ◽  
PETER J. H. SHARPE ◽  
JOHN D. GOESCHL

2021 ◽  
Author(s):  
Yifan Yuan ◽  
Cynthia Sung

Abstract Additive manufacturing provides a rapid manufacturing method for a variety of materials with different applications. Thermoplastic Polyurethane (TPU) is a soft polymer material that can be 3D printed. In this work, we explore the mechanical properties of a 3D printed grid pattern structure with TPU. By changing the pattern’s cell size and wall thickness parameters, we control the density of the grid lattice and, as a result, the bulk elastic modulus of the structure. We compare simulation and physical compression tests and conclude that the bulk elastic modulus of a print is related to the infill percentage according to a cubic relationship, with higher infill percentage samples resulting in higher elastic moduli. The precise cell size and wall thickness parameter values are minor influences comparatively. The elastic moduli of the resulting samples span from 0.36 MPa with 23.44% infill to 21.83 MPa with 75% infill, compared to an elastic modulus of 64.31 MPa when printing at 100% density. We also explore other factors such as the sample size, the printer, the build orientation, and the sample geometry. The results have uses in a variety of applications, including a custom linear spring, a bistable gripper, or a soft robot finger.


2000 ◽  
Vol 649 ◽  
Author(s):  
G. Feng ◽  
A.H.W. Ngan

ABSTRACTDuring the unloading segment of nanoindentation, time dependent displacement (TDD) accompanies elastic deformation. Consequently the modulus calculated by the Oliver-Pharr scheme can be overestimated. In this paper we present evidences for the influence of the measured modulus by TDD. A modification method is also presented to correct for the effects of TDD by extrapolating the TDD law in the holding process to the beginning of the unloading process. Using this method, the appropriate holding time and unloading rate can be estimated for nanoindentation test to minimise the effects of TDD. The elastic moduli of three materials computed by the modification method are compared with the results without considering the TDD effects.


2020 ◽  
Vol 7 (5) ◽  
pp. 1421-1426 ◽  
Author(s):  
Zhenfeng Guo ◽  
Akira Shinohara ◽  
Chengjun Pan ◽  
Florian J. Stadler ◽  
Zhonghua Liu ◽  
...  

Bulky but flexible alkyl side chains enable π-conjugated polymers to possess wide-range elastic modulus tuneability, yet consistent red luminescent properties.


Author(s):  
Bouzid Choubane ◽  
Chung-Lung Wu ◽  
Mang Tia

The results of a laboratory testing program carried out to investigate the effect of coarse aggregate types on the elastic modulus of typical pavement concretes are presented. The elastic modulus was determined in both flexure and compression using static and dynamic means. Three different mixes, made using three different aggregates, were compared. The water-cement ratio was kept at 0.53 throughout the test program. The results showed that within the tested range, the aggregate type significantly affected the studied properties of concrete. Calera aggregate (a dense limestone) with its rough-textured surface and angular shape produced a concrete with higher strength and stiffness than those of concretes made with Brooksville aggregate (a porous limestone) and river gravel. In addition, the measured dynamic modulus in compression was significantly different from that in flexure. Also, in flexure, the dynamic modulus was higher than the static modulus by an average of 23 percent, whereas in compression, the dynamic modulus appeared to be in the same range as the static modulus. The change in frequency from 1 to 7 Hz did not have a significant influence on the dynamic modulus.


2019 ◽  
Vol 218 (4) ◽  
pp. 1408-1421 ◽  
Author(s):  
Xiaohui Liu ◽  
Jiazhou Li ◽  
Heyu Zhao ◽  
Boyang Liu ◽  
Thomas Günther-Pomorski ◽  
...  

Even though cell walls have essential functions for bacteria, fungi, and plants, tools to investigate their dynamic structure in living cells have been missing. Here, it is shown that changes in the intensity of the plasma membrane dye FM4-64 in response to extracellular quenchers depend on the nano-scale porosity of cell walls. The correlation of quenching efficiency and cell wall porosity is supported by tests on various cell types, application of differently sized quenchers, and comparison of results with confocal, electron, and atomic force microscopy images. The quenching assay was used to investigate how changes in cell wall porosity affect the capability for extension growth in the model plant Arabidopsis thaliana. Results suggest that increased porosity is not a precondition but a result of cell extension, thereby providing new insight on the mechanism plant organ growth. Furthermore, it was shown that higher cell wall porosity can facilitate the action of antifungal drugs in Saccharomyces cerevisiae, presumably by facilitating uptake.


Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 51 ◽  
Author(s):  
Meghan Robinson ◽  
Karolina Valente ◽  
Stephanie Willerth

We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Neurons require soft substrates to differentiate and mature, however measuring the elastic moduli of soft substrates remains difficult to accurately measure using standard protocols such as atomic force microscopy or shear rheology. Here we validate a direct method for acquiring elastic modulus of fibrin using a modified Hertz model for thin films. In this method, spherical indenters are positioned on top of the fibrin samples, generating an indentation depth that is then correlated with elastic modulus. Neurons function by transmitting electrical signals to one another and being able to assess the development of electrical signaling serves is an important verification step when engineering neural tissues. We then validated a protocol wherein the electrical activity of motor neural cultures is measured directly by a voltage sensitive dye and a microplate reader without causing damage to the cells. These protocols provide a non-destructive method for characterizing the mechanical and electrical properties of living spinal cord tissues using novel biosensing methods.


2004 ◽  
Vol 19 (8) ◽  
pp. 2437-2447 ◽  
Author(s):  
J.W. Leggoe

Nanoindentation of the reinforcement in a particulate reinforced metal matrix composite (PR MMC) enables direct investigation of reinforcement properties within the finished material. Mismatch between the elastic moduli of the reinforcement and matrix creates a “secondary indentation” effect, whereby the stiffer reinforcement particles themselves “indent” the more compliant matrix. A finite-element investigation was undertaken to quantify the additional penetration arising under secondary indentation for spherical and cylindrical particles. Modification of Sneddon’s equation for a flat punch by a scalar particle shape factor provided an accurate estimate of the additional penetration. The modified equation was combined with the analysis of Field and Swain to extract the particle elastic modulus from results obtained using a spherical indenter under a multiple partial-unloading indentation regime. The resulting methodology was used to determine the elastic moduli of silicon carbide particles and MicralTM microspheres in two aluminum-matrix PR MMCs.


Geophysics ◽  
1996 ◽  
Vol 61 (2) ◽  
pp. 444-455 ◽  
Author(s):  
Jack Dvorkin ◽  
Amos Nur ◽  
Caren Chaika

Our observations made on dry‐sandstone ultrasonic velocity data relate to the variation in velocity (or modulus) with effective stress, and the ability to predict a velocity for a rock under one effective pressure when it is known only under a different effective pressure. We find that the sensitivity of elastic moduli, and velocities, to effective hydrostatic stress increases with decreasing porosity. Specifically, we calculate the difference between an elastic modulus, [Formula: see text], of a sample of porosity ϕ at effective pressure [Formula: see text] and the same modulus, [Formula: see text], at effective pressure [Formula: see text]. If this difference, [Formula: see text], is plotted versus porosity for a suite of samples, then the scatter of ΔM is close to zero as porosity approaches the critical porosity value, and reaches its maximum as porosity approaches zero. The dependence of this scatter on porosity is close to linear. Critical porosity here is the porosity above which rock can exist only as a suspension—between 36% and 40% for sandstones. This stress‐sensitivity pattern of grain‐supported sandstones (clay content below 0.35) practically does not depend on clay content. In practical terms, the uncertainty of determining elastic moduli at a higher effective stress from the measurements at a lower effective stress is small at high porosity and increases with decreasing porosity. We explain this effect by using a combination of two heuristic models—the critical porosity model and the modified solid model. The former is based on the observation that the elastic‐modulus‐versus‐porosity relation can be approximated by a straight line that connects two points in the modulus‐porosity plane: the modulus of the solid phase at zero porosity and zero at critical porosity. The second one reflects the fact that at constant effective stress, low‐porosity sandstones (even with small amounts of clay) exhibit large variability in elastic moduli. We attribute this variability to compliant cracks that hardly affect porosity but strongly affect the stiffness. The above qualitative observation helps to quantitatively constrain P‐ and S‐wave velocities at varying stresses from a single measurement at a fixed stress. We also show that there are distinctive linear relations between Poisson’s ratios (ν) of sandstone measured at two different stresses. For example, in consolidated medium‐porosity sandstones [Formula: see text], where the subscripts indicate hydrostatic stress in MPa. Linear functions can also be used to relate the changes (with hydrostatic stress) in shear moduli to those in compressional moduli. For example, [Formula: see text], where [Formula: see text] is shear modulus and [Formula: see text] is compressional modulus, both in GPa, and the subscripts indicate stress in MPa.


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