Accumulation of an adcrusting substance in the pigment strand of a diploid and a tetraploid wheat

1975 ◽  
Vol 53 (19) ◽  
pp. 2246-2250 ◽  
Author(s):  
S.-Y. Zee
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Functional Significance ◽  
Tetraploid Wheat ◽  
Wheat Grain ◽  
Pigment Strand ◽  
Filling Mechanism

The pattern of accumulation of the sudanophilic substance on the wall of the cells of the pigment strand of a diploid and a tetraploid wheat was followed by high-resolution light microscopy. The cells of the pigment strand of the 4n wheat tend to elaborate and accumulate more adcrusting substance in the cells of the pigment strand than the 2n wheat. The functional significance of the accumulation of the adcrusting substance in relation to the filling mechanism of the wheat grain is discussed.

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

Correlative video enhanced differential interference contrast light microscopy (VDIC)-LM), low-voltage high-resolution SEM (LV-HR-SEM), and high-voltage electron microscopy (HVEM) in the observation of gold-labelled surface antigens and the subjacent cytoskeletal matrix

1989 ◽  
Vol 47 ◽  
pp. 904-905
Author(s):  
Ralph M. Albrecht ◽  
Scott R. Simmons ◽  
Marek Malecki
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Low Voltage ◽  
Microscopic Analysis ◽  
Cell Labelling ◽  
Video Technology ◽  
Electron Microscopic ◽  
Image Capture ◽  
High Voltage Electron Microscopy ◽  
Fluorescence Studies

The development of video-enhanced light microscopy (LM) as well as associated image processing and analysis have significantly broadened the scope of investigations which can be undertaken using (LM). Interference/polarization based microscopies can provide high resolution and higher levels of “detectability” especially in unstained living systems. Confocal light microscopy also holds the promise of further improvements in resolution, fluorescence studies, and 3 dimensional reconstruction. Video technology now provides, among other things, a means to detect differences in contrast difficult to detect with the human eye; furthermore, computerized image capture, processing, and analysis can be used to enhance features of interest, average images, subtract background, and provide a quantitative basis to studies of cells, cell features, cell labelling, and so forth. Improvements in video technology, image capture, and cost-effective computer image analysis/processing have contributed to the utility and potential of the various interference and confocal microscopic instrumentation.Electron microscopic technology has made advances as well. Microprocessor control and improved design have contributed to high resolution SEMs which have imaging capability at the molecular level and can operate at a range of accelerating voltages starting at 1KV. Improvements have also been seen in the HVEM and IVEM transmission instruments. As a whole, these advances in LM and EM microscopic technology provide the biologist with an array of information on structure, composition, and function which can be obtained from a single specimen. Corrrelative light microscopic analysis permits examination of living specimens and is critical where the “history” of a cell, cellular components, or labels needs to be known up to the time of chemical or physical fixation. Features such as cytoskeletal elements or gold label as small as 0.01 μm, well below the 0.2 μm limits of LM resolution, can be “detected” and their movement followed by VDIC-LM. Appropriate identification and preparation can then lead to the examination of surface detail and surface label with stereo LV-HR-SEM. Increasing the KV in the HR-SEM while viewing uncoated or thinly coated specimens can provide information from beneath the surface as well as increasing Z contrast so that positive identification of surface and subsurface colloidal gold or other heavy metal labelled/stained material is possible. Further examination of the same cells using stereo HVEM or IVEM provides information on internal ultrastructure and on the relationship of labelled material to cytoskeletal or organellar distribution, A wide variety of investigations can benefit from this correlative approach and a number of instrumentational configurations and preparative pathways can be tailored for the particular study. For a surprisingly small investment in time and technique, it is often possible to clear ambiguities or questions that arise when a finding is presented in the context of only one modality.

High-Resolution Fiber and Fiber Tract Imaging Using Polarized Light Microscopy in the Human, Monkey, Rat, and Mouse Brain

2016 ◽  
pp. 369-389 ◽  
Author(s):  
Karl Zilles ◽  
Nicola Palomero-Gallagher ◽  
David Gräßel ◽  
Philipp Schlömer ◽  
Markus Cremer ◽  
...  
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Mouse Brain ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Fiber Tract

scholarly journals High-resolution computational imaging of leaf hair patterning using polarized light microscopy

2013 ◽  
Vol 73 (4) ◽  
pp. 701-708 ◽  
Author(s):  
Marcelo Pomeranz ◽  
Jeffrey Campbell ◽  
Dan Siegal-Gaskins ◽  
Jacob Engelmeier ◽  
Tyler Wilson ◽  
...  
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Polarized Light ◽  
Computational Imaging ◽  
Polarized Light Microscopy

scholarly journals Ultrastructure of the Developing Aleurone Cells of Wheat Grain

1963 ◽  
Vol 16 (4) ◽  
pp. 768 ◽  
Author(s):  
MS Buttrose
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Cell Walls ◽  
Osmium Tetroxide ◽  
Aleurone Layer ◽  
Wheat Grain ◽  
Aleurone Cells ◽  
Wheat Kernel ◽  
Large Numbers ◽  
Inner Surface

The developing aleurone layer cells of the wheat kernel have been investigated by electron microscopy and the results compared with those of light microscopy. Two weeks after flowering vacuoles appear in the cells and deposits accumulate in these until maturity when the cells are filled 'with the resulting "vacuolar units" 2-3p. in diameter, corresponding to the aleurone grains of light microscopy. The wheat aleurone grain consists of a bounding membrane (of vacuole origin) enclosing a matrix in which are embedded spherical deposits. Some of these deposits are translucent and others opaque to electrons after potassium permanganate and osmium tetroxide fixation. At all stages examined the cytoplasm of aleurone cells contained large numbers of small unidentified bodies with irregular outline and dense contents. At first they are dispersed, but towards maturity are organized as a monolayer over the surface of each aleurone grain and the inner surface of the cell walls. The apparent specificity of these structures to aleurone cells is discussed in relation to future chemical and physiological studies of the tissue.

scholarly journals High-resolution Light Microscopy (HRLM) and Digital Analysis of Pompe Disease Pathology

2005 ◽  
Vol 53 (1) ◽  
pp. 63-73 ◽  
Author(s):  
Colleen M. Lynch ◽  
Jennifer Johnson ◽  
Charles Vaccaro ◽  
Beth L. Thurberg
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Pompe Disease ◽  
Digital Analysis

scholarly journals Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Astha Jaiswal ◽  
Christian H. Hoerth ◽  
Ana M. Zúñiga Pereira ◽  
Holger Lorenz
Keyword(s):  
Endoplasmic Reticulum ◽  
Cost Effectiveness ◽  
High Resolution ◽  
Light Microscopy ◽  
Matrix Protein ◽  
Super Resolution ◽  
Ease Of Use ◽  
Live Cells ◽  
Cell Organelles ◽  
Morphology Changes

Abstract Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locations within the endoplasmic reticulum, Golgi and mitochondria can reliably be established. We also show the benefit of this approach for organelle quantifications, especially for clumped or intertwined organelles like peroxisomes and mitochondria. Since cell and organelle swelling is reversible, it can be applied to live cells for successive high-resolution analyses. Our approach outperforms many existing imaging modalities with respect to resolution, ease-of-use and cost-effectiveness without excluding any co-utilization with existing optical (super)resolution techniques.

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