In vitro germination of Entomophthora aphidis resting spores

1975 ◽  
Vol 53 (12) ◽  
pp. 1188-1191 ◽  
Author(s):  
David Tyrrell ◽  
Donald M. MacLeod
Keyword(s):  
Germ Tube ◽  
Terminal Cell ◽  
In Vitro Germination ◽  
Germination Process ◽  
Resting Spores ◽  
Nutrient Reserves

On germination, resting spores of Entomophthora aphidis produce a septate sporogenous germ tube, which in turn gives rise to two or occasionally three germ conidia, one conidium being formed by the terminal cell and the others from cells lower down the tube. The resting spores possess sufficient endogenous nutrient reserves to complete the germination process.

In vitro germination of Entomophthora egressa resting spores

1976 ◽  
Vol 54 (10) ◽  
pp. 1131-1134 ◽  
Author(s):  
Richard A. Nolan ◽  
Gary B. Dunphy ◽  
Donald M. MacLeod
Keyword(s):  
Spore Germination ◽  
Germ Tube ◽  
In Vitro Germination ◽  
Effect Of Ph ◽  
High Ph ◽  
Ph Values ◽  
Resting Spore ◽  
Resting Spores ◽  
Hyphal Bodies

The resting spores of Entomophthora egressa MacLeod and Tyrrell germinated to produce a single germ tube, which in turn, produced a solitary, terminal germ conidium. The level of resting spore germination obtained in shaken, liquid, steam-sterilized media after 12 h of incubation at pH 7.5 was 93%. The effect of pH on resting spore germination in filter-sterilized media was investigated; in the range pH 4.5 to 9.5, pH 8.5 and 9.5 were optimal (100% germination after 96 h). No germination occurred at pH 4.5 to 6.5 before 96 h. At values greater than pH 6.5, the proportion of resting spores which germinated to form a single germ tube with a solitary germ conidium decreased, and the resting spores increasingly germinated by forming one to five hyphae. At high pH values (pH 7.5 to 9.5) septa occurred in the hyphae after 120 h of incubation. This latter phenomenon may have been correlated with the occurrence of hyphal bodies in shaken media at high pH (pH 8.5). The germination of resting spores to form hyphae at high pH levels may indicate the presence of a mechanism for infection via the gut.

Effect of light exposure on in vitro germination and germ tube growth of eight species of rust fungi

Mycologia ◽  
2010 ◽  
Vol 102 (5) ◽  
pp. 1134-1140 ◽  
Author(s):  
James W. Buck ◽  
Weibo Dong ◽  
Daren S. Mueller
Keyword(s):  
Germ Tube ◽  
Light Exposure ◽  
Rust Fungi ◽  
Tube Growth ◽  
In Vitro Germination ◽  
Effect Of Light ◽  
Germ Tube Growth

The effect of temperature on in vitro germination and germ tube growth of urediniospores of Tranzschelia discolor

1990 ◽  
Vol 41 (3) ◽  
pp. 479 ◽  
Author(s):  
PJ Ellison ◽  
BR Cullis ◽  
RW Bambach ◽  
PF Kable
Keyword(s):  
Germ Tube ◽  
Three Dimensional ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Three Dimensional Models ◽  
C 30 ◽  
Tranzschelia Discolor ◽  
Germ Tube Growth

The effect of temperature on in vitro germination and germ tube growth of urediniospores of Tranzschelia discolor was studied over time under constant temperature conditions. Studies were carried out on 1% water agar in the dark at 3�C, 5�C, 8�C, 10�C, 15�C, 20�C, 25�C, 28�C, 30�C and 32�C. Germination was observed at all temperatures between 5 and 30'C, and occurred rapidly over most of this range. At 2 h, germination exceeded 80% at temperatures between 10 and 28�C, and this level was reached at 3 h at 8�C. Germination at 5 and 30�C was much reduced and at 7 h reached only 44% and 38% respectively. Germ tube growth occurred most vigorously at 15 and 20�C, reaching lengths in excess of 500 8m at 9 h. The optimum range was narrower than that for germination, and growth was reduced or poor at 8�C, 10�C, 25�C and 28�C, which were favourable temperatures for germination. Average germ tube lengths at 9 h at these temperatures were 55, 245, 273 and 62 8m, respectively. Three-dimensional models were derived relating germination and germ tube growth to time and temperature.

scholarly journals Pecan Pollen Stored Over a Decade Retains Viability

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 176-177 ◽  
Author(s):  
Darrell Sparks ◽  
I.E. Yates
Keyword(s):  
Liquid Nitrogen ◽  
Pollen Morphology ◽  
Germ Tube ◽  
Pollen Grains ◽  
Carya Illinoinensis ◽  
In Vitro Germination ◽  
Fresh Pollen

In vitro germination of freshly collected pollen and pollen stored 1, 10, 11, 12, and 13 years in liquid nitrogen was examined for `Desirable' pecan [Carya illinoinensis (Wangenh.) C. Koch]. Viability of pollen stored in liquid nitrogen for 1, 10, 11, 12, and 13 years was not diminished in comparison to that of fresh pollen. Morphology of stored pollen grains and the germ tube was normal. Thus, liquid nitrogen may offer a means of haploid preservation of pecan.

The effect of light and temperature on in vitro germination and germ tube growth of urediniospores of Tranzschelia discolor

1992 ◽  
Vol 43 (3) ◽  
pp. 451 ◽  
Author(s):  
PJ Ellison ◽  
BR Cullis ◽  
PF Kable
Keyword(s):  
Light Intensity ◽  
Germ Tube ◽  
Tube Growth ◽  
Tube Length ◽  
In Vitro Germination ◽  
Germ Tube Length ◽  
Tranzschelia Discolor ◽  
Effect Of Light ◽  
Germ Tube Growth

The effect of light on in vitro germination of urediniospores of Tranzschelia discolor was studied over time at different intensitities (up to 400 8E m-2 s-l) within the temperature range 5�C to 20�C. A model was also developed from the data to predict germination at different combinations of light, temperature and times of leaf wetness. Light retarded the germination process, and its effect increased in direct proportion to intensity. At 20�C, for example, the time taken to exceed 80% germination increased from 2 h in the dark to 9 h at 200 8E m-2 s-l. The model showed that there was an interaction between light and temperature, with the effect of light becoming more pronounced as the temperature declined below 20�C. Germination percentages of the order of 90% were, however, recorded within 24 h at all combinations of light intensity and temperature studied. Light also influenced germ tube growth, causing a reduction in the rate of growth. As in germination, its effect increased with increasing light intensity. At 20�C, the average germ tube length at 9 h was 541 8m in the dark, compared with 227 8m at 200 8E m-2 s-1 and 148 8m at 400 8E m-2 s-l. A similar effect was observed at 5�C, where the average germ tube length at 24 h was 274 8m in the dark compared with 157 8m at 200 8E m-2 s-l. The effects of light on the germination and germ tube growth of urediniospores under field conditions are discussed.

scholarly journals Natural Compounds That Modulate the Development of the Fungus Botrytis cinerea and Protect Solanum lycopersicum

Plants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 111 ◽  
Author(s):  
Esteban D. Rosero-Hernández ◽  
Javier Moraga ◽  
Isidro G. Collado ◽  
Fernando Echeverri
Keyword(s):  
Cell Viability ◽  
Botrytis Cinerea ◽  
Mechanism Of Action ◽  
Germ Tube ◽  
Tomato Fruit ◽  
Natural Compounds ◽  
Gray Mold ◽  
In Vitro Germination ◽  
High Cell

Botrytis cinerea is the causal agent of gray mold disease and is responsible for the loss of millions of dollars in crops in worldwide. Currently, this pathogen exhibits increasing resistance to conventional fungicides; therefore, better control methods and novel compounds with a more specific mechanism of action but without biocidal effects, are required. In this work, several natural compounds to control B. cinerea were analyzed in vitro. Detected effects were dependent on the stage of fungus development, and 3-phenyl-1-propanol displayed the most potent inhibition of in vitro germination, germ tube development, and sporulation. However, it had lower protection of leaves and postharvest fruit in plant infection. Isoeugenol and 1-phenylethanol exhibited lower inhibition of in vitro germination and sporulation, but at the highest concentrations, they inhibited germ tube elongation. Although the lowest rates of foliage infection were recorded using isoeugenol and 3-phenyl-1-propanol, 1-phenylethanol significantly decreased the disease in postharvest tomato fruit, with an efficacy like Mancozeb, but at 18 times lower micromolar concentration. All compounds resulted in high cell viability after spores were removed from the treatment solution exhibited high cell viability, suggesting a non-biocidal effect. The diversity of in vitro and in-plant effects seems to indicate a different mechanism of action.

scholarly journals Optimization of jenipapo in vitro seed germination process

2016 ◽  
Vol 40 (6) ◽  
pp. 658-664 ◽  
Author(s):  
Rafaela Ribeiro de Souza ◽  
Patrícia Duarte de Oliveira Paiva ◽  
Raphael Reis da Silva ◽  
Michele Valquíria dos Reis ◽  
Fernanda Carlota Nery ◽  
...  
Keyword(s):  
Seed Germination ◽  
Light Quality ◽  
Culture Media ◽  
Distilled Water ◽  
In Vitro Germination ◽  
Continuous Darkness ◽  
Fluorescent Lamps ◽  
Germination Process ◽  
Internal And External Factors

ABSTRACT The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA3; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L-1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.

In vitro germination protocol for the propagation of the endangered butternut tree (Juglans cinerea L.)

2019 ◽  
Vol 20 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Martin Williams ◽  
Kathleen Forbes ◽  
Charlene Williams ◽  
Tannis Beardmore
Keyword(s):  
In Vitro Germination ◽  
Juglans Cinerea

scholarly journals Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

2006 ◽  
Vol 108 (1) ◽  
pp. 91-94 ◽  
Author(s):  
J. Lora ◽  
M.A. Pérez de Oteyza ◽  
P. Fuentetaja ◽  
J.I. Hormaza
Keyword(s):  
Low Temperature ◽  
In Vitro Germination ◽  
Low Temperature Storage ◽  
Annona Cherimola ◽  
Temperature Storage
Sign in / Sign up

Export Citation Format

Share Document