The influence of soil water extract and thiamine on the growth of Cladophora glomerata
Soil water extract (SWE) has been used for decades to supplement certain algal and fungal cultures, but its active components remain essentially unknown. Cladophora glomerata, an attached green alga which has become a problem in several of the Laurentian Great Lakes and elsewhere, is one species that grows well on synthetic media supplemented with SWE. Unialgal cultures were used to investigate the nature of the active components in SWE with the hope that insight of the nutritional requirements of this alga would be gained.Analysis of SWE showed that an organic heat-labile component was significant, and when thiamine was substituted for SWE, it was found to provide 80% of the stimulation induced by SWE. The presence of thiamine in two samples of SWE was verified, and concentrations of 3 and 10 μg/litre were determined using the Phycomyces bioassay. Subsequent investigations of the requirement of thiamine by C. glomerata demonstrated that 1 μg/litre, provided either by SWE or as the pure vitamin, stimulated satisfactory growth, while 10 μg/litre is the apparent optimum concentration.