Scanning electron microscopy of meiotic chromosomes of plants in situ

1974 ◽  
Vol 52 (6) ◽  
pp. 1438-1440 ◽  
Author(s):  
Ernest D. P. Whelan ◽  
G. H. Haggis ◽  
E. J. Ford ◽  
B. Dronzek
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Solanum Tuberosum ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Solanum Tuberosum L ◽  
Meiotic Spindle ◽  
Meiotic Chromosomes ◽  
Scanning Electron

Scanning electron microscope studies of anthers of the Asiatic Lilium hybrid Enchantment and Solanum tuberosum L. cv. Netted Gem fixed in organic acid – alcohol type fixatives clearly revealed nucleoli, bivalents, and the meiotic spindle. Centromere regions could not be identified in pachytene bivalents, but areas of possible spindle attachment were evident for metaphase I – anaphase I bivalents.

Scanning electron microscopy of the callose wall and intermeiocyte connections in angiosperms

1974 ◽  
Vol 52 (6) ◽  
pp. 1215-1218 ◽  
Author(s):  
Ernest D. P. Whelan ◽  
G. H. Haggis ◽  
E. J. Ford
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Solanum Tuberosum ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Plasma Membranes ◽  
Nuclear Region ◽  
Lupinus Polyphyllus ◽  
Callose Wall ◽  
Scanning Electron

Scanning electron microscope studies of anthers of the dicotyledons Helianthus annuus, Solanum tuberosum, and Lupinus polyphyllus, and the monocotyledons Iris pseudo-acoris and a Lilium hybrid revealed discontinuities or holes in the meiocyte callose wall and continuity of the plasma membranes of adjacent meiocytes. The holes in the callose wall generally were confined to areas where neighboring meiocytes were in contact. The holes varied in size within locations and between taxa. The largest holes, about 2.4 μm diameter, were found in Lupinus. Fixation in standard acid–alcohol fixatives resulted in marked plasmolysis and loss of cytoplasmic detail, but the nucleolus and bivalents were readily apparent. Fixation in buffered glutaraldehyde, with or without postfixation in OSU4, preserved the cytoplasmic organelles and plasmolysis was minimal, but bivalents could not be distinguished. All fixatives preserved the nuclear membrane so that the nuclear region was clearly delimited from the cytoplasm.

Preparing plant chromosomes for scanning electron microscopy

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 333-339 ◽  
Author(s):  
John E. Dillé ◽  
Douglas C. Bittel ◽  
Kathleen Ross ◽  
J. Perry Gustafson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Situ Hybridization ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Plant Chromosomes ◽  
Cellular Debris ◽  
Scanning Electron

The scanning electron microscope may be useful in the analysis of plant chromosomes treated with in situ hybridization, especially when the probes and (or) chromosomes are near or beyond the resolution of the light microscope. Usual methods of plant chromosome preparation are unsuitable for scanning electron microscope observation as a result of cellular debris, which also interferes with probe hybridization. A method is described whereby protoplasts are obtained from fixed root tips by enzymatic digestion and applied to slides in a manner that produces little or no cellular debris overlying the chromosomes. The slides were examined by scanning electron microscopy and light microscopy after C-banding and in situ hybridization with a rye nucleolus organizer region spacer probe. This technique, which allows for scanning electron microscope visualization of bands and probes not easily identified with light microscopy, should prove useful in the physical mapping of low copy number or unique DNA sequences.Key words: protoplasts, rice, wheat, rye, physical maps, in situ hybridization.

High Resolution Scanning Electron Microscopy

1991 ◽  
Vol 49 ◽  
pp. 478-479
Author(s):  
David Joy ◽  
James Pawley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Beam ◽  
Electron Microscope ◽  
High Resolution ◽  
Scanning Electron Microscope ◽  
Electron Probe ◽  
Impact Point ◽  
Interaction Volume ◽  
Scanning Electron

The scanning electron microscope (SEM) builds up an image by sampling contiguous sub-volumes near the surface of the specimen. A fine electron beam selectively excites each sub-volume and then the intensity of some resulting signal is measured. The spatial resolution of images made using such a process is limited by at least three factors. Two of these determine the size of the interaction volume: the size of the electron probe and the extent to which detectable signal is excited from locations remote from the beam impact point. A third limitation emerges from the fact that the probing beam is composed of a finite number of discrete particles and therefore that the accuracy with which any detectable signal can be measured is limited by Poisson statistics applied to this number (or to the number of events actually detected if this is smaller).

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

The External Morphology of the Eggs of Culex (Culex) saltanensis (Diptera: Culicidae) Under Scanning Electron Microscopy

2020 ◽  
Author(s):  
J R Santos-Mallet ◽  
T D Balthazar ◽  
A A Oliveira ◽  
W A Marques ◽  
A Q Bastos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Optical Microscopy ◽  
Osmium Tetroxide ◽  
Neotropical Region ◽  
External Morphology ◽  
Metal Supports ◽  
Scanning Electron

Abstract The aim of the present study was to describe the morphology of the eggs of Culex (Culex) saltanensis Dyar that occurs in the Neotropical region. Eggs of the Cx. (Cux.) saltanensis were collected at the Mata Atlântica FIOCRUZ campus, fixed in 1% osmium tetroxide, prepared for mounting on metal supports, observed under a scanning electron microscope, and described morphologically. The eggs had a coniform shape with a length of approximately 0.5 mm (505–510 µm) and a width in the median portion of 117 µm (113–123 µm). Upper portion is lined with tubers of irregular shape and varying sizes (0.64–1.31 µm), located on a cross-linked matrix forming bands observed under optical microscopy. The micropyle is encased in a necklace of approximately 6.6-µm plates arranged in a flower-like shape. Comparing Cx. (Cux.) saltanensis eggs with several species of different genera, important divergent characteristics can be observed. However, this study points to the need for new descriptions of eggs of species belonging to the same subgenus in order to analyze if there will be differences between them. Culex (Cux.) saltanensis eggs have particular characteristics not observed in eggs of other Culicidae genera.

Preparation of Alcohol-Preserved Larvae of Culicidae (Diptera) for Scanning Electron Microscopy

1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Taxonomic Studies ◽  
Scanning Electron ◽  
Sem Studies

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.

Scanning electron microscope studies of miracidia suggest introgressive hybridization between Schistosoma haematobium and S. haematobium x S. mattheei in the Eastern Transvaal

1988 ◽  
Vol 62 (2) ◽  
pp. 141-147 ◽  
Author(s):  
F. J. Kruger ◽  
V. L. Hamilton-Attwell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Schistosoma Haematobium ◽  
Introgressive Hybridization ◽  
Human Infection ◽  
Infection Rates ◽  
High Prevalence ◽  
Scanning Electron

ABSTRACTSchistosoma haematobium miracidia were collected from a locality with a high prevalence of human infection with the animal parasite, S. mattheei, which hybridizes with S. haematobium, and from 2 localities with negligible infection rates. The terebratoria of the miracidia from these localities were compared with each other, with laboratory maintained S. haematobium and with four populations of S. mattheei by means of scanning electron microscopy. It was found that the terebratorial membrane of certain of the S. haematobium miracidia from the locality with a high S. mattheei prevalence in humans, resembled the more intricate membrane of S. mattheei. This suggests introgressive hybridization between S. haematobium and S. haematobium x S. mattheei.

scholarly journals Capture and scanning electron microscopy of individual snow crystals

1994 ◽  
Vol 40 (134) ◽  
pp. 195-197
Author(s):  
E. W. Wolff ◽  
A. P. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Liquid Nitrogen Temperature ◽  
Experimental Methods ◽  
Snow Crystal ◽  
Snow Crystals ◽  
First Time ◽  
Scanning Electron

AbstractA snow crystal has been successfully collected on to a scanning electron microscope (SEM) stub in central Greenland. It was preserved at liquid-nitrogen temperature for 5 months, prior to examination in the SEM. This is believed to be the first time a snow crystal has been observed directly in the SEM and offers some new experimental methods for understanding crystals and their chemistry.

scholarly journals Scanning Electron Microscopy Observations of Loa loa (Nematoda)

2020 ◽  
Vol 11 (2) ◽  
pp. 486-492
Author(s):  
Jens Anibal Juul ◽  
Vegard Asgeir Forsaa ◽  
Tor Paaske Utheim ◽  
Endre Willassen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Case Report ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Loa Loa ◽  
Scanning Electron

We present a case report of periocular Loa loa. The key feature of L. loa distinguishing it from other human filarial parasites are cuticular bosses, which are presented in images from a light microscope and a scanning electron microscope. The cuticular bosses could be divided into three subtypes not previously described.

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