Ultrastructure of callus tissue of Zea mays

1974 ◽  
Vol 52 (1) ◽  
pp. 251-254 ◽  
Author(s):  
Judy W. Johnson ◽  
David J. Holden

Corn coleoptile nodal tissue was used to study the callusing of corn inbred and hybrid varieties. A comparison of callus cells and coleoptile parenchyma cells was made with electron micrographs. The callus cells resembled meristematic cells in having more ribosomes, and had a greater number of mitochondria and plastids than nodal parenchyma cells. There was a high incidence of lomasome-like bodies in both types of cells. The callus cells were characterized by having small vacuoles scattered throughout the cell, whereas the nodal parenchyma tissue was composed of cells with mostly a single large vacuole surrounded by a thin parietal cytoplasm.

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hwan-Hee Bae ◽  
Gibum Yi ◽  
Young Sam Go ◽  
Jun Young Ha ◽  
Yuchan Choi ◽  
...  

AbstractCorn (Zea mays L.) provides a major source of calories for human consumption and therefore, the nutritional components of corn have a large impact on human health. For example, corn kernels contain antioxidants, such as polyphenols (including anthocyanins and other flavonoids) and carotenoids. Such compounds represent useful targets for biofortification breeding. In this study, we used 34 corn inbred lines from three different regions (East Asia, Southern Asia, and subtropical regions) and 11 F1 hybrids derived from the inbreds to investigate antioxidant activity in yellow corn. We compared different methods for measuring antioxidant activity to test their consistency and to determine whether color could be used as an indicator of antioxidant activity. We also measured carotenoid levels in yellow corn. No difference in antioxidant activity was detected between inbred corn lines from temperate vs. tropical regions. We determined that carotenoid is a major contributor to antioxidant activity in yellow corn and that kernel color, especially yellowness, could be used as an indicator of antioxidant activity in yellow corn. These findings lay the foundation for the biofortification of yellow corn by providing information about the correlations among kernel color, carotenoid contents, and antioxidant activity and by identifying an easy method to assess antioxidant activity in yellow corn.


2001 ◽  
Vol 81 (1) ◽  
pp. 75-76
Author(s):  
L. M. Reid ◽  
G. McDiarmid ◽  
A. J. Parker ◽  
R. I. Hamilton

CO429 is a short-season corn inbred line with good combining ability, average to superior lodging resistance in hybrids, and excellent emergence and early season vigour. Key words: Corn, maize, Zea mays, cultivar description


2018 ◽  
Vol 98 (5) ◽  
pp. 1212-1219
Author(s):  
L.M. Reid ◽  
C. Voloaca ◽  
J. Wu ◽  
T. Woldemariam ◽  
K.K. Jindal ◽  
...  

CO463 is a short-season corn (Zea mays L.) inbred line with excellent combining ability with both Iodent and stiff stalk inbred testers, as well as intermediate resistance to common rust, eyespot, common smut, and Fusarium stalk rot.


2016 ◽  
Vol 96 (3) ◽  
pp. 387-391
Author(s):  
Lana M. Reid ◽  
Xiaoyang Zhu ◽  
Constantin Voloaca ◽  
Jinhe Wu ◽  
Tsegaye Woldemariam ◽  
...  

CO455 is a short season stiff stalk-type corn (Zea mays L.) inbred line with excellent combining ability as well as intermediate resistance to common smut, common rust, eyespot and fusarium stalk rot. Excellent hybrid yields and performance data were achieved when CO455 was combined with Iodent testers such as MBS8148.


Weed Science ◽  
1991 ◽  
Vol 39 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Giuseppe Forlani ◽  
Erik Nielsen ◽  
Pierangelo Landi ◽  
Roberto Tuberosa

Seven corn inbred lines previously shown to differ in response to soil residues of chlorsulfuron were characterized as to the target-enzyme acetolactate synthase (ALS) specific activity and to its susceptibility to the herbicide. ALS from plantlets at the five-leaf stage of growth was similarly susceptible to chlorsulfuron in all lines and its specific activity in the shoots was not significantly correlated with in vivo tolerance to the herbicide. By contrast, differences in ALS specific activity in roots of plants both at the five- and three-leaf stages of growth were significantly correlated (r = 0.96∗∗and r = 0.93∗∗, respectively) with in vivo tolerance. Correlation was also noted in extracts from cultured excised root tips (r = 0.94∗∗). Callus tissue of a chlorsulfuron-tolerant line was less affected by the herbicide and had a significantly higher ALS specific activity than callus from a chlorsulfuron susceptible line, whereas inhibition of ALS by the herbicide was similar in both lines. These results indicate that the naturally occurring differences in ALS levels in the roots of the investigated inbred lines contribute largely to the differential in vivo response observed to chlorsulfuron.


1975 ◽  
Vol 53 (5) ◽  
pp. 427-431 ◽  
Author(s):  
L. E. Pelcher ◽  
K. N. Kao ◽  
O. L. Gamborg ◽  
O. C. Yoder ◽  
V. E. Gracen

Leaf protoplasts from resistant (N, C, and S cytoplasm) and susceptible (T cytoplasm) corn inbred W182B (Zea mays L.) exhibited a differential response after exposure to Helminthosporium maydis race T toxin. The volume of untreated protoplasts increased twofold during the first 24 h after isolation and by 48–72 h, most protoplasts exhibited an extensive network of cytoplasmic strands and the chloroplasts were distributed throughout the cytoplasm by cyclosis. However, susceptible protoplasts exposed to ≥2 μg toxin/ml failed to increase in volume and cytoplasmic streaming was rarely observed. By 72 h there was extensive degradation and collapse of susceptible protoplasts exposed to toxin. Resistant protoplasts exposed to up to 100 μg toxin/ml exhibited a twofold volume increase and were indistinguishable from untreated protoplasts. Susceptible protoplasts exposed to 0.1 μg toxin/ml also appeared unaffected, but at 1.0 μg toxin/ml an intermediate response was observed. The differential response to H. maydis race T toxin of protoplasts from resistant and susceptible corn correlates well with those effects observed in intact plant tissues and may serve to explain further the mode of action of the toxin on susceptible corn cells.


2014 ◽  
Vol 94 (8) ◽  
pp. 1529-1533
Author(s):  
L. M. Reid ◽  
C. Voloaca ◽  
J. Wu ◽  
T. Woldemariam ◽  
K. Jindal ◽  
...  

Reid, L. M., Voloaca, C., Wu, J., Woldemariam, T., Jindal, K. and Zhu, X. 2014. CO454 corn inbred line. Can. J. Plant Sci. 94: 1529–1533. CO454 is a short-season corn (Zea mays L.) inbred line with excellent combining ability as well as moderate resistance to common smut and rust. Excellent hybrid yields and performance data were achieved when CO454 was combined with stiff stalk inbreds such as TR3030 and MBS1130 as well as with the Iodent inbred MBS8148.


IAWA Journal ◽  
2015 ◽  
Vol 36 (4) ◽  
pp. 400-408 ◽  
Author(s):  
Kishore S. Rajput ◽  
Sangeeta Gupta

Successive cambia are often associated with the climbing or shrub habit, and is less common in trees. We studied formation of successive cambia and structure of secondary xylem in young stems of Cocculus laurifolius DC., a tree species of Menispermaceae. Cell division in the vascular cambium ceased in pencil-thick stems. Subsequently, parenchyma cells located outside the perivascular fibre cap re-differentiated and gave rise to several small segments of meristematic cells, of which the central cells divided repeatedly to initiate the first successive cambium which produces secondary xylem centripetally and phloem centrifugally. Cells located on the inner side of the newly initiated cambium differentiated into conjunctive tissue while cells on the outer side of it divided further and differentiated into sclereids. Xylem was diffuse porous and composed of vessels, fibre tracheids and ray parenchyma cells, and only differed in vessel diameter from wide-vessel climbing relatives.


2018 ◽  
Vol 9 (2) ◽  
pp. 577
Author(s):  
Tirumala Jawahar Sri Gopi ◽  
A. Yuvaraja ◽  
R. Naveena ◽  
O. S. Kruthika

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