Isoperoxidases of (IAA oxidase) oxidase in oat coleoptiles

1973 ◽  
Vol 51 (11) ◽  
pp. 2047-2052 ◽  
Author(s):  
William R. Gordon ◽  
James H. M. Henderson
Keyword(s):  
Acetic Acid ◽  
Avena Sativa ◽  
Gel Electrophoresis ◽  
Enzyme Assay ◽  
Polyacrylamide Gel Electrophoresis ◽  
Acid Oxidase ◽  
Iaa Oxidase ◽  
Substrate Specificities ◽  
Electrophoretic Mobilities ◽  
Indole 3 Acetic Acid

Eight constitutive isoperoxidases were separated by the disc method of polyacrylamide gel electrophoresis from a lyophilized extract of 8-day-old oat (Avena sativa L., cv. Victory) coleoptiles. Both anodic and cathodic isoperoxidases were studied and differences in electrophoretic mobilities and hydrogen donor substrate specificities were revealed. In addition, by enzyme assay, cathodic and anodic isoenzymes were shown to possess differences in peroxidase and IAA (indole-3-acetic acid) oxidase activities.Treatment of coleoptiles with 0.07 mM IAA for 24 h resulted in the repression of two slow-migrating anodic isoperoxidases; however, the same treatment also resulted in the induction of two slow-migrating cathodic isoenzymes which were shown to exhibit peroxidase and IAA oxidase activities.

Effects of Auxin-Like Herbicides on Nucleohistones in Cucumber and Wheat Roots

Weed Science ◽  
1973 ◽  
Vol 21 (3) ◽  
pp. 181-184 ◽  
Author(s):  
L. G. Chen ◽  
A. Ali ◽  
R. A. Fletcher ◽  
C. M. Switzer ◽  
G. R. Stephenson
Keyword(s):  
Triticum Aestivum ◽  
Protein Synthesis ◽  
Acetic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Wheat Roots ◽  
Anisic Acid ◽  
Cucumber Roots ◽  
Dichlorophenoxy Acetic Acid ◽  
Dna Template

Roots of susceptible cucumber (Cucumis sativusL. ‘Chicago pickling’) and tolerant wheat (Triticum aestivumL. ‘Manitou’) seedlings were treated with 3,6-dichloro-o-anisic acid (dicamba) or (2,4-dichlorophenoxy)acetic acid (2,4-D) for either 10 or 46 hr. The roots were excised and nucleohistones were isolated and fractionated by polyacrylamide gel electrophoresis. In untreated cucumber roots there were four major nucleohistone fractions. Two of these fractions decreased or were not detectable 10 or 46 hr after treatment with dicamba or 2,4-D. In wheat, the nucleohistone fractions of the treated roots were similar to those of the controls. This suggests that in cucumber more of the DNA template is available for transcription. This suggestion was supported by the fact that the incorporation of14C-adenine into RNA in cucumber roots 10 hr after treatment with dicamba was increased by 50%, whereas in wheat there was no difference. Furthermore, the incorporation of14C-leucine into protein in cucumber roots 10 hr after treatment with dicamba was inhibited by 70%, indicating that the increased RNA produced was incapable of translating for protein synthesis. It is proposed that selective phytotoxicity of auxin-like herbicides is based on a differential alteration of RNA species and interference with protein synthesis.

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