A simplified method for isolating bacteria-free crown gall tissue from Vinca rosea

1971 ◽  
Vol 49 (7) ◽  
pp. 1255-1257 ◽  
Author(s):  
Robert J. Manasse ◽  
Jacques Lipetz
Keyword(s):  
Antibiotic Resistance ◽  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Water Bath ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Simplified Method ◽  
Crown Gall Tissue ◽  
Temperature Controlled ◽  
Simplified Procedure

A simplified procedure for the isolation of bacteria-free crown gall tissues requiring only a temperature-controlled water bath is described. Agrobacterium tumefaciens recovered from stem sections during this procedure retain their tumor-inducing properties as well as their antibiotic resistance.

The biosynthesis of cytokinins in crown-gall tissue of Vinca rosea

Planta ◽  
1979 ◽  
Vol 147 (2) ◽  
pp. 97-102 ◽  
Author(s):  
T. Stuchbury ◽  
L. M. Palni ◽  
R. Horgan ◽  
P. F. Wareing
Keyword(s):  
Crown Gall ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Crown Gall Tissue

Mass spectrometric measurement of zeatin glycoside levels in Vinca rosea L. crown gall tissue

Planta ◽  
1982 ◽  
Vol 154 (3) ◽  
pp. 273-276 ◽  
Author(s):  
I. M. Scott ◽  
G. C. Martin ◽  
R. Horgan ◽  
J. K. Heald
Keyword(s):  
Crown Gall ◽  
Mass Spectrometric ◽  
Mass Spectrometric Measurement ◽  
Spectrometric Measurement ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Crown Gall Tissue

Zeatin-9-glucoside, a major endogenous cytokinin of Vinca rosea L. crown gall tissue

Planta ◽  
1980 ◽  
Vol 149 (5) ◽  
pp. 472-475 ◽  
Author(s):  
I. M. Scott ◽  
R. Horgan ◽  
B. A. McGaw
Keyword(s):  
Crown Gall ◽  
Endogenous Cytokinin ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Crown Gall Tissue

Cytokinins in transfer RNA of normal and crown-gall tissue of Vinca rosea

Planta ◽  
1983 ◽  
Vol 159 (2) ◽  
pp. 178-181 ◽  
Author(s):  
L. M. S. Palni ◽  
R. Horgan
Keyword(s):  
Crown Gall ◽  
Transfer Rna ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Crown Gall Tissue

Cytokinin Accumulation in the Culture Medium of Vinca rosea L. Crown-Gall Tissue: a Time-Course Study

1984 ◽  
Vol 11 (3) ◽  
pp. 129 ◽  
Author(s):  
LMS Palni
Keyword(s):  
Crown Gall ◽  
Time Course ◽  
Culture Medium ◽  
Zeatin Riboside ◽  
Significant Inhibition ◽  
Tissue Growth ◽  
Cytokinin Activity ◽  
Gall Tissue ◽  
Vinca Rosea ◽  
Crown Gall Tissue

Cytokinin activity was detected in the culture medium of Vinca rosea (Catharanthus roseus (L.) G. Don) crown-gall tumour tissue 1 week after subculture. Activity increased with time, reaching a plateau at 5-6 weeks. The cytokinin activity from extracts of staled culture medium after 1 and 2 weeks culture cochromatographed with zeatin, zeatin riboside and their dihydro derivatives. An additional slow-moving peak of cytokinin glucoside-like activity was observed from 3 weeks after subculture, and was detectable even at the end of a 10-week culture period. When [U-14C]adenine was supplied to V. rosea crown-gall tissue, analysis of radioactivity remaining in the medium showed incorporation of label into zeatin and zeatin riboside (trans-isomers only) after only 1 h of incubation. Radioactivity in these compounds increased with time; peaking at 8 h, and was still detectable 125 h after incubation. At no time was radioactivity detected in dihydrozeatin, isopentenyladenine or their ribosides. At the end of 125 h incubation, less than 0.2% of radioactivity supplied initially was still present as adenine in the medium. Significant inhibition of tissue growth occurred when it was cultured on medium supplemented with a synthetic or naturally occumng cytokinin.

Occurrence of 3-Indolylacetic and 3-Indolecarboxylic Acids in Tomato Crown—Gall Tissue Extracts

Nature ◽  
1959 ◽  
Vol 184 (4689) ◽  
pp. 825-826 ◽  
Author(s):  
G. CLARKE ◽  
M. H. DYE ◽  
R. L. WAIN
Keyword(s):  
Crown Gall ◽  
Gall Tissue ◽  
Tissue Extracts ◽  
Crown Gall Tissue
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