Media for growth of flax tissue culture

1971 ◽  
Vol 49 (2) ◽  
pp. 295-298 ◽  
Author(s):  
R. K. Ibrahim
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Root Formation ◽  
Callus Tissue ◽  
Maximum Growth ◽  
Shoot Formation ◽  
Tissue Differentiation ◽  
Naphthalene Acetic Acid ◽  
Mineral Salts ◽  
Callus Tissue Culture

A callus tissue culture from flax cotyledons reached maximum growth on a medium rich in mineral salts and supplemented with 2 mg/l α-naphthalene acetic acid, 0.05 mg/l kinetin, 15% coconut milk, and 3% sucrose. While tissue differentiation was evident and root formation was induced with increasing auxin concentrations, higher kinetin levels or the addition of L-tyrosine failed to promote shoot formation.

Tissue culture and genetic analysis of somaclonal variations of Solanum melongena L. cv. Nirrala

2014 ◽  
Vol 9 (12) ◽  
pp. 1182-1195
Author(s):  
Samar Naseer ◽  
Tariq Mahmood
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Soluble Protein ◽  
Solanum Melongena ◽  
Total Soluble Protein ◽  
Naphthalene Acetic Acid ◽  
Fresh Tissue ◽  
Somaclonal Variations ◽  
Random Amplification ◽  
Plant Grown

AbstractThe present study was designed to analyze genetically somaclonal variants using biochemical and molecular markers. Efficient tissue culture protocol for Solanum melongena L. cv. Nirrala was developed. Maximum callus induction (100%) was observed for Murashige and Skoog (MS) media supplemented with 2.0 mg L−1 naphthalene acetic acid +0.5 mg L−1 6-benzylaminopurine; and nodal explants gave best callusing response (88.8%) as compared to internodes (88.3%) and leaves (87.7%). The best shooting was induced on nodal and internodal callus in the presence of 2.0 mg L−1 6-benzylaminopurine. Total soluble protein content of callus and regenerated variant plants was estimated for biochemical analysis, and largest amount of soluble protein was found in callus (6.54 mg g−1 fresh tissue) followed by variant plant grown on 2.0 mg L−1 6-benzylaminopurine (5.96 mg g−1 fresh tissue). Random amplification of polymorphic DNA technique was done with five decamer primers (OPC1-OPC5) and maximum polymorphism was detected by OPC 2 (26.99%) among all samples, whereas nodal callus on media containing 1.0 mg L−1 naphthalene acetic acid +1.0 mg L−1 6-benzylaminopurine showed highest polymorphism producing 22 bands, out of which 8 bands were polymorphic. The study shows that this marker system can provide better evaluation of genetic variation induced by tissue culture.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Variation of axillary growth as respond of Morus spp. micropropagation using various concentration of Indonesian local solid substance

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Yasinta Ratna Esti Wulandari ◽  
Laurensia Danis Anggradita
Keyword(s):  
Growth Rate ◽  
Tissue Culture ◽  
Acetic Acid ◽  
Callus Formation ◽  
Culture Technique ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Solid Substance ◽  
Local Plant ◽  
Morus Spp

Abstract. Wulandari YRE, Anggradita LD. 2020. Variation of axillary growth as respond of Morus spp. micropropagation using various concentrations of Indonesian local solid substance. Biodiversitas 21: 80-85. The difficulties of growing Morus spp. makes it become one local plant that hard to cultivate conventionally even though it’s a beneficiary plant. Hence cultivation Morus spp. through tissue culture technique could help growing this plant. This research is aimed to design the optimal condition for micropropagation of local Morus spp. (Morus bombycis var. lembang, M. cathayana, M. multicaulis, and M. alba var. kanva-2) using agar-agar as Indonesian local solid substance. This solid substance is used as its cheap and easy to find compared to other solid substances. This research used MS medium supplemented with 0.1 ppm naphthalene acetic acid + 1.0 ppm benzyl aminopurine and various concentration of agar-agar (0.6%, 0.8%, 1.0%). Growth rate, axillary bud length and number, leaf number, callus formation and contamination were observed in this research. All those concentrations could be used for micropropagation of Morus spp. Agar concentrations of 0.8 and 1.0% showed better results than 0.6% because it showed the highest results.

scholarly journals Studying on the in vitro shoot formation of Opuntia ficus – indica (L.) Mill.

2019 ◽  
Vol 3 (1) ◽  
pp. 18-28
Author(s):  
Nguyen Thi Cam Duyen ◽  
Bui Trang Viet ◽  
Tran Thanh Huong
Keyword(s):  
Acetic Acid ◽  
Respiration Rate ◽  
Shoot Formation ◽  
Photosynthesis Rate ◽  
Naphthalene Acetic Acid ◽  
Opuntia Ficus Indica ◽  
Shoot Height ◽  
Explant Position ◽  
Meristematic Region

This study presented the elimination of the glochids in the areoles of Opuntia ficus-indica cladode induced the shoot formation from the explants containing the shoot apical meristem (SAM) on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) 5 mg/L. Morphological and physiological changes in shoot formation process were analyzed. This process involved the constant stages: activation of cell division, initiating of meristematic region; formation of shoot primordium and shoot with leaves. The SAM position in the upper front of the cladode gave the highest shoot productivity. This position had the photosynthesis rate, respiration rate, endogenous indole acetic acid (IAA) and zeatin activity higher than the others. Difference of auxin concentrations increased the amount of shoots and shoot height. The maximum number of shoots per explant was obtained on MS medium supplemented with BA 5 mg/L and 1-naphthalene acetic acid (NAA) 0.5 mg/L. The elimination of SAM by removing the surface of the shoot gave the highest number of the shoot. The correlation of the glochids in the areoles, explant position, plant hormones, photosynthesis rate respiration rate and shoot formation were discussed.  

FERN CALLUS TISSUE CULTURE

1951 ◽  
Vol 38 (2) ◽  
pp. 141-143 ◽  
Author(s):  
G. Morel ◽  
R. H. Wetmore
Keyword(s):  
Tissue Culture ◽  
Callus Tissue ◽  
Callus Tissue Culture

scholarly journals INFLUNCE OF AUXIN AND POLYAMINEES ON ROOTING OF SHOOTS OF CITRUS VOLKAMERIANA ROOTSTOCK IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
College Of Agriculture ◽  
Indole Butyric Acid ◽  
Citrus Volkameriana

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influence  of  combinations  of Indole  butyric  acid (IBA) ,  Naphthalene  acetic  acid (NAA) and  polyamine Spermidine (Spd.) on rooting of shoots of  citrus volkameriana rootstock cultured  on 1\2  MS medium. The Results indicated that 1/2 MS medium supplemented  with 1.0 mg L-1 (IBA)  gave the highest  percentage  of  rooting  (67 %) which differed significantly  from the MS medium with free auxin IBA  that gave (22%) while  the  MS medium  supplemented with 0.5 mg L-1  spermidine  gave the highest percentage of rooting (63%) that was not significantly different  than other concentrations of Spd . MS medium supplemented with 1.0 mg L-1 IBA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (83%) and the highest root number / shoot (3.17) and highest length of root (3.15 cm) while the MS medium with free auxin IBA and spd. did not give percentage of rooting (0%) for citrus volkameriana rootstock plantlets . The MS medium supplemented with 1.0 mg L-1 NAA gave the highest percentage of rooting (56%) which differed significantly from MS medium with free auxin NAA that gave (22%)  while MS medium supplemented with 0.5mg L-1 spd. gave the highest percentage of rooting (50%) that was not significantly different from other concentration of Spd . MS medium supplemented with 1.0 mg L-1 NAA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (68%) and the highest root number /shoot (2.5) and highest length of root (2.65 cm) while the MS medium with free auxin NAA and Spd. did not gave percentage of rooting (0%) .

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

2015 ◽  
Vol 23 (2) ◽  
pp. 451-455 ◽  
Author(s):  
Sheng Hu ◽  
Yingying Ma ◽  
Hailong Jiang ◽  
Dingjun Feng ◽  
Wei Yu ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Callus Tissue ◽  
Paeonia Lactiflora ◽  
Callus Tissue Culture
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