Photosynthesis and photorespiration rates at the CO2 compensation point

1970 ◽  
Vol 48 (6) ◽  
pp. 1271-1276 ◽  
Author(s):  
N. R. Bulley ◽  
E. B. Tregunna

Attached soybean leaves in a closed system were fed high specific activity 14CO2 at four CO2 compensation points (30, 53, 110, 204 μl/l CO2) which were produced by different O2 concentrations (10, 21, 44, 80% O2). Rates of CO2 uptake and evolution were measured simultaneously using an infrared CO2 gas analyzer and a Geiger tube. The CO2 compensation point was proportional to O2 concentration but did not change for the two light intensities used. The rates of CO2 exchange at the compensation point did increase with light intensity. These rates of CO2 exchange also increased with the increasing CO2 concentration of the compensation point (for increasing O2 concentrations) but tended to saturate for compensation points greater than 110 μl/l CO2.

1970 ◽  
Vol 48 (6) ◽  
pp. 1251-1257 ◽  
Author(s):  
N. P. Voskresenskaya ◽  
G. S. Grishina ◽  
S. N. Chmora ◽  
N. M. Poyarkova

Apparent photosynthesis of attached leaves of Phaseolus vulgaris, Vicia faba, Pisum sativum, and Nicotiana tabacum at various intensities of blue and red light was measured by infrared CO2 gas analyzer in a closed system. Simultaneously the CO2 compensation point was measured.It was found that light-limited photosynthetic rate in blue light was equal to or more than that in red light. Inhibition of photosynthesis, which sometimes occurred at light-saturated intensities of blue light, could be avoided by addition of red light, prolonged exposure of the plants to blue light, or by lowering the O2 concentration. Accordingly, the increase of photosynthetic rate due to change of O2 concentration from 21 to 3% O2 is higher in blue light only when photosynthesis is inhibited by blue light at 21% O2. The data on the action of blue and red light on the CO2 compensation point seems to exclude the activation of photorespiration by blue light.The possible effects of blue light on apparent photosynthesis are discussed on the basis of the results presented.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 583b-583 ◽  
Author(s):  
Trinidad Reyes ◽  
Terril A. Nell ◽  
Charles A. Conover ◽  
James E. Barrett

Effects of three light intensities (564, 306 and 162 μmol m-2 s-1) and three fertilizer rates (220, 440 and 880 mg/15 cm pot, weekly) were evaluated on acclimatization potential of Chamaedorea elegans. Treatments were applied during four months under greenhouse conditions after which plants were placed indoors (20 μmol m-2 s-1, 21±2C and 50% RH) for two months. Light compensation point (LCP) was significantly reduced by decreasing light intensity and increasing fertilizer rates. Leaf and root fresh and dry weights increased with irradiance while shoots were not affected. Chlorophyll a levels were higher in plants grown under the lowest light intensity. Carbohydrate content is being analyzed and anatomical examination of leaves studied. Plant performance indoors will be discussed. These studies demonstrate that Chamaedorea, a monocot, acclimatizes similarly to dicots.


1977 ◽  
Vol 55 (6) ◽  
pp. 639-643 ◽  
Author(s):  
F. D. H. Macdowall

The maximum rate coefficients for the vegetative phase of growth in dry weight of shoots and roots were independent of the photoperiod in plants grown at six different light intensities of 12- or24-h duration at 20 °C. The strict dependence of plant growth on only the total daily incident radiant energy was proved by the superposition of hyperbolic plots of growth coefficients obtained for five different photoperiods and for the different light intensities. Root growth was very sensitive to photoperiod at moderate light intensity. Growth kinetics changed from first to zero order in plant dry weight at a 12-h photoperiod with a light intensity of 150 ft-c (3750 ergs cm−2 s−1) depending on pretreatment. The light compensation point of plant growth occurred at16 × 107 ergs cm−2 day−1; that for shoots was lower, and that for roots was higher.


1982 ◽  
Vol 47 (03) ◽  
pp. 244-248 ◽  
Author(s):  
D P Thomas ◽  
Rosemary E Merton ◽  
T W Barrowcliffe ◽  
L Thunberg ◽  
U Lindahl

SummaryThe in vitro and in vivo characteristics of two oligosaccharide heparin fragments have been compared to those of unfractionated mucosal heparin. A decasaccharide fragment had essentially no activity by APTT or calcium thrombin time assays in vitro, but possessed very high specific activity by anti-Factor Xa assays. When injected into rabbits at doses of up to 80 ¼g/kg, this fragment was relatively ineffective in impairing stasis thrombosis despite producing high blood levels by anti-Xa assays. A 16-18 monosaccharide fragment had even higher specific activity (almost 2000 iu/mg) by chromogenic substrate anti-Xa assay, with minimal activity by APTT. When injected in vivo, this fragment gave low blood levels by APTT, very high anti-Xa levels, and was more effective in preventing thrombosis than the decasaccharide fragment. However, in comparison with unfractionated heparin, the 16-18 monosaccharide fragment was only partially effective in preventing thrombosis, despite producing much higher blood levels by anti-Xa assays.It is concluded that the high-affinity binding of a heparin fragment to antithrombin III does not by itself impair venous thrombogenesis, and that the anti-Factor Xa activity of heparin is only a partial expression of its therapeutic potential.


1962 ◽  
Vol 08 (03) ◽  
pp. 425-433 ◽  
Author(s):  
Ewa Marciniak ◽  
Edmond R Cole ◽  
Walter H Seegers

SummarySuitable conditions were found for the generation of autoprothrombin C from purified prothrombin with the use of Russell’s viper venom or trypsin. DEAE chromatographed prothrombin is structurally altered and has never been found to yield autoprothrombin C and also did not yield it when Russell’s viper venom or trypsin were used. Autoprothrombin C is derived from prothrombin with tissue extract thromboplastin, but not in large amounts with the intrinsic clotting factors. With the latter thrombin and autoprothrombin III are the chief activation products. Autoprothrombin III concentrates were prepared from serum and upon activation with 25% sodium citrate solution or with Russell’s viper venom large amounts of autoprothrombin C were obtained, and this was of high specific activity. Theoretically trypsin is not a thrombolytic agent, but on the contrary should lead to intravascular clotting.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jingying Zhang ◽  
Changhai Sui ◽  
Huimin Liu ◽  
Jinjiao Chen ◽  
Zhilin Han ◽  
...  

Abstract Background ‘Regal Splendour’ (Hosta variety) is famous for its multi-color leaves, which are useful resources for exploring chloroplast development and color changes. The expressions of chlorophyll biosynthesis-related genes (HrHEMA, HrPOR and HrCAO) in Hosta have been demonstrated to be associated with leaf color. Herein, we isolated, sequenced, and analyzed HrHEMA, HrPOR and HrCAO genes. Subcellular localization was also performed to determine the location of the corresponding enzymes. After plasmid construction, virus-induced gene silencing (VIGS) was carried out to reduce the expressions of those genes. In addition, HrHEMA-, HrPOR- and HrCAO-overexpressing tobacco plants were made to verify the genes function. Changes of transgenic tobacco were recorded under 2000 lx, 6000 lx and 10,000 lx light intensity. Additionally, the contents of enzyme 5-aminolevulinic acid (5-ALA), porphobilinogen (PBG), chlorophyll a and b (Chla and Chlb), carotenoid (Cxc), superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), proline (Pro) and catalase (CAT) under different light intensities were evaluated. Results The silencing of HrHEMA, HrPOR and HrCAO genes can induce leaf yellowing and chloroplast structure changes in Hosta. Specifically, leaves of Hosta with HrCAO silencing were the most affected, while those with HrPOR silencing were the least affected. Moreover, all three genes in tobacco were highly expressed, whereas no expression was detected in wild-type (WT). However, the sensitivities of the three genes to different light intensities were different. The highest expression level of HrHEMA and HrPOR was detected under 10,000 lx of illumination, while HrCAO showed the highest expression level under 6000 lx. Lastly, the 5-ALA, Chla, Cxc, SOD, POD, MDA, Pro and CAT contents in different transgenic tobaccos changed significantly under different light intensities. Conclusion The overexpression of these three genes in tobacco enhanced photosynthesis by accumulating chlorophyll content, but the influential level varied under different light intensities. Furthermore, HrHEMA-, HrPOR- and HrCAO- overexpressing in tobacco can enhance the antioxidant capacity of plants to cope with stress under higher light intensity. However, under lower light intensity, the antioxidant capacity was declined in HrHEMA-, HrPOR- and HrCAO- overexpressing tobaccos.


2021 ◽  
pp. 1-7
Author(s):  
Michael A. Reichenberger ◽  
Jagoda M. Urban-Klaehn ◽  
Jason V. Brookman ◽  
Joshua L. Peterson-Droogh ◽  
Jorge Navarro ◽  
...  

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