Behavior in vitro of plant cells from various sources within the same organism

1969 ◽  
Vol 47 (8) ◽  
pp. 1334-1336 ◽  
Author(s):  
W. G. Barker
Keyword(s):  
Triticum Aestivum ◽  
Callus Culture ◽  
Single Unit ◽  
Plant Cells ◽  
Triticum Aestivum L ◽  
The Body ◽  
Cultural Environment ◽  
Culture Bottle ◽  
General Appearance

Explants derived from widely divergent tissues within the body of the basswood, Tilia americana L. (ovary wall, root and shoot cambia, perimedullary region, petiole), have the same general appearance when growing as a callus culture on identical media. The cambial-derived calli will meld into a single unit when placed in contact in the same culture bottle. Similarly, calli from several sources of wheat, Triticum aestivum L., are indistinguishable in vitro.From the standpoint of purely observational criteria, parenchyma explanted from several organs within the same organism responds in a seemingly identical way to the cultural environment.

scholarly journals Genes control of rates development as components of regulation stability of Triticum aestivum L. to biotic stress under conditions in vitro

1970 ◽  
pp. 159-163
Author(s):  
O. O. Avksentiieva ◽  
N. V. Terentiieva
Keyword(s):  
Triticum Aestivum ◽  
Fusarium Oxysporum ◽  
Callus Culture ◽  
Biotic Stress ◽  
Growth Index ◽  
Triticum Aestivum L ◽  
Isogenic Lines ◽  
The Impact

Aim. The aim of our work is to research the influence of exometabolites phytopathogens of genus Fusarium on callus culture isogenic lines for genes PPD (NILs) of winter soft wheat. Methods. In the work used standard biotechnological and mycological methods. The influence of exometabolites phytopathogens g. Fusarium investigated, adding CF mi-cromycetes to MS culture medium in a ratio of 1:20, using transplants callus culture of isogenic lines of wheat. Growth index analyzed density of callus tissue and size of callus cells determined. Results. Established that the CF of Fusarium oxysporum significantly slows grows reaction of callus cultures and morphological structure callus NILs is changes. It is shown that the impact exometabolites phytopathogens has the opposite effect on cytological parameters (number and length callus cells) in the isolines differing pace of development in conditions in vivo. The culture filtrate F. oxysporum has more toxicity compared to exometabolites F. moniliforme. Conclusions. It is supposed that the genetic system controlling the pace of development and photoperiodic sensitivity Triticum aestivum L. in conditions in vivo indirectly determines the formation of resistance to biotic stress conditions in vitro. Keywords: Triticum aestivum L., Fusarium oxysporum, Fusarium moniliforme, PPD genes, NILs, callus culture, growth index, resistance to phytopathogens.

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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