Circadian rhythms of flower induction and their significance in photoperiodic response
Endogenous rhythms of flower induction in Chenopodium rubrum provide at least a partial basis for time measurement and control of flower induction in daily light: dark (photoperiodic) cycles. The results support Bünning's hypothesis of the physiological clock.The following three intrinsic components can exert a primary control in both the endogenous free-running and the externally forced system.(1) GenotypeThe endogenous rhythmic flowering responses of different latitudinal ecotypes show specific characteristics that are also expressed in daily photoperiodic cycles.(2) PhytochromeChanges in the level of Pfr (the active form of phytochrome) cause shifts in endogenous phase and photoperiodic optima that are qualitatively similar. Changes in Pfr level have effects on flower induction that are similar in both the photophile phase of an endogenous rhythm and in the light period of inductive daily photoperiodic cycles—just as the effects obtained in the transduction (skotophile) phase of the endogenous rhythm are similar to those obtained in the dark period of daily photoperiodic cycles.(3) Photosynthetic Energy MetabolismThe effects on flower induction obtained by changing light intensity and (or) quality, and by applying sugars, suggest that there is a greater utilization and (or) availability of substrates (arising from carbohydrate metabolism and involved in Pfr pacemaker effects) in the photophile than in the transduction phases of prolonged darkness. This appears to reflect the sequence that would normally be imposed in daily photoperiodic cycles.A basis for the overt relationships between endogenous rhythmic and photoperiodic response could exist if there are endogenous oscillations in energy metabolites derived from photosynthesis. Such a system could have become selectively evolved as an adaptive response to natural alternations of light and darkness. Demultiplication of higher frequency oscillations may be a significant adaptive mechanism in circadian timing of flower induction.