ELECTRON MICROSCOPY OF UREDOSPORES OF MELAMPSORA LINI AND OF RUST-INFECTED FLAX

1967 ◽  
Vol 45 (9) ◽  
pp. 1575-1582 ◽  
Author(s):  
M. S. Manocha ◽  
Michael Shaw
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Linum Usitatissimum ◽  
Room Temperature ◽  
Rust Fungus ◽  
Thin Sections ◽  
Infection Structures ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Germ Tubes

Thin sections of germinated and ungerminated uredospores of the flax rust fungus (Melampsora lini (Pers.) Lev.) and rust-infected cotyledons of flax (Linum usitatissimum L., variety Bison) were examined in a Philips 100B electron microscope. The line structure of the mature uredospores, the formation of germ tubes including the development of cross walls, and of the intercellular mycelium, haustoria, sporogenous hyphae, and developing uredospores are all briefly described. Germinated and ungerminated uredospores were fixed in KMnO4 followed by OsO4 at room temperature. Infected tissue was fixed in KMnO4 at room temperature or in glutaraldehyde at 4 °C followed by OsO4 at 4 °C. Nucleoli were not observed in the nuclei of mature uredospores or germ tubes but were present in the intercellular mycelium, sporogenous hyphae, and developing uredospores and were particularly prominent in the two haustorial nuclei. The results are discussed and attention is drawn to the need for further work on the nuclei and nucleoli in germinating uredospores under conditions conducive to the development of infection structures.

Frozen Sectioning of Porous Polymer Membranes for Electron Microscopy

1968 ◽  
Vol 26 ◽  
pp. 412-413
Author(s):  
E. F. Koch
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Room Temperature ◽  
Polymer Membranes ◽  
Polymer Membrane ◽  
Porous Polymer ◽  
Thin Sections ◽  
The One ◽  
Diamond Knife

A technique has been devised using a diamond knife ultramicrotome in conjunction with a freezing head to prepare thin sections of porous polymer membranes. The thin sections are examined and photographed in transmission with an electron microscope to reveal the hole sizes in the membranes.Attempts to microtome a particular type of porous polymer membrane at room temperature proved unsuccessful, since the polymer membrane was not strong enough to support Itself when cut to appropriate viewing thicknesses of about 250 Å to 500 Å. It was thought that some degree of rigidity could be imparted to the polymer by cooling it below room temperature. Therefore, a freezing head was made for the Porter-Blum microtome, similar to the one developed by Crudgington, Fig. 1.

Lattice imaging and pore structure of β ferric oxyhydroxide

1978 ◽  
Vol 36 (1) ◽  
pp. 264-265
Author(s):  
T. Baird ◽  
J.R. Fryer ◽  
S.T. Galbraith
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Bulk Material ◽  
Model Structure ◽  
Bulk Crystals ◽  
Lattice Imaging ◽  
Thin Sections ◽  
Ferric Oxyhydroxide ◽  
Resolution Electron ◽  
Hydrolysis Of

Introduction Previously we had suggested (l) that the striations observed in the pod shaped crystals of β FeOOH were an artefact of imaging in the electron microscope. Contrary to this adsorption measurements on bulk material had indicated the presence of some porosity and Gallagher (2) had proposed a model structure - based on the hollandite structure - showing the hollandite rods forming the sides of 30Å pores running the length of the crystal. Low resolution electron microscopy by Watson (3) on sectioned crystals embedded in methylmethacrylate had tended to support the existence of such pores.We have applied modern high resolution techniques to the bulk crystals and thin sections of them without confirming these earlier postulatesExperimental β FeOOH was prepared by room temperature hydrolysis of 0.01M solutions of FeCl3.6H2O, The precipitate was washed, dried in air, and embedded in Scandiplast resin. The sections were out on an LKB III Ultramicrotome to a thickness of about 500Å.

Observations of thick and thin sections in a field-emission SEM

1994 ◽  
Vol 52 ◽  
pp. 1018-1019
Author(s):  
W. P. Wergin ◽  
S. Roy ◽  
E. F. Erbe ◽  
C. A. Murphy ◽  
C. D. Pooley
Keyword(s):  
Electron Microscope ◽  
Field Emission ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Chemical Fixation ◽  
Thin Sections ◽  
Transmission Electron ◽  
Conventional Transmission Electron Microscope ◽  
Scanning Electron

Larvae of the nematode, Steinernema carpocapsae Weiser strain All, were cryofixed and freezesubstituted for 3 days in acetone containing 2% osmium tetroxide according to established procedures. Following chemical fixation, the nematodes were brought to room temperature, embedded in Spurr's medium and sectioned for observation with a Hitachi S-4100 field emission scanning electron microscope that was equipped with an Oxford CT 1500 Cryotrans System. Thin sections, about 80 nm thick, similar to those generally used in conventional transmission electron microscope (TEM) studies were mounted on copper grids and stained with uranyl acetate for 30 min and lead citrate for 5 min. Sections about 2 μm thick were also mounted and stained in a similar fashion. The grids were mounted on an Oxford grid holder, inserted into the microscope and onto a cryostage that was operated at ambient temperature. Thick and thin sections of the larvae were evaluated and photographed in the SEM at different accelerating voltages. Figs. 4 and 5 have undergone contrast conversion so that the images would resemble transmitted electron micrographs obtained with a TEM.

CDC Bethune flax

2002 ◽  
Vol 82 (1) ◽  
pp. 101-102 ◽  
Author(s):  
G. G. Rowland ◽  
Y. A. Hormis ◽  
K. Y. Rashid
Keyword(s):  
Linum Usitatissimum ◽  
Oil Content ◽  
Oil Quality ◽  
High Yield ◽  
Lodging Resistance ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Crop Development ◽  
Cultivar Description ◽  
Moderately Resistant

CDC Bethune, is a medium-late-maturing oilseed flax (Linum usitatissimum L.) developed by Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar has medium oil content, medium oil quality, medium seed size, good lodging resistance and high yield when seeded early in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description

CDC Buryu oilseed flax

2020 ◽  
Author(s):  
Helen M. Booker ◽  
Gordon Rowland ◽  
Hadley R. Kutcher ◽  
khalid Y. rashid
Keyword(s):  
Linum Usitatissimum ◽  
Oil Content ◽  
Oil Quality ◽  
Black Soil ◽  
Lodging Resistance ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Crop Development ◽  
Moderately Resistant ◽  
Maturity Rating

CDC Buryu, a late maturing oilseed flax (Linum usitatissimum L.), was registered in 2016 by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar had yield (106%) comparable to CDC Bethune and a maturity rating equal to Flanders in all soil zones of the Northern Prairies. The yield advantage over CDC Bethune was greater (>8%) in the Brown soil and Black soil zones of the Northern Prairies. It had medium oil content, oil quality, and seed size and good lodging resistance. It is immune to North American rust (race 371) caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. Lini and powdery mildew caused by Oidium lini.

AC Emerson flax

1996 ◽  
Vol 76 (3) ◽  
pp. 483-485 ◽  
Author(s):  
E. O. Kenaschuk ◽  
K. Y. Rashid ◽  
G. H. Gubbels
Keyword(s):  
Key Words ◽  
Linum Usitatissimum ◽  
Oil Quality ◽  
Calcareous Soils ◽  
Research Centre ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Early Maturing ◽  
Cultivar Description ◽  
Moderately Resistant

AC Emerson, a medium-early maturing oilseed flax (Linum usitatissimum L.), was released by Agriculture and Agri-Food Canada, Agri-Food Diversification Research Centre, Morden, Manitoba, in 1994. The cultivar has high oil quality, medium-large seed size and was high yielding in both early and late seeding tests in the Black and the Brown Soil zones of Manitoba and Saskatchewan. Because of its chlorosis tolerance, it is particularly well suited to the calcareous soils of Manitoba. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description

AC Watson flax

1998 ◽  
Vol 78 (3) ◽  
pp. 465-466 ◽  
Author(s):  
E. O. Kenaschuk ◽  
K. Y. Rashid
Keyword(s):  
Linum Usitatissimum ◽  
Oil Quality ◽  
Research Centre ◽  
Lodging Resistance ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Early Maturing ◽  
Cultivar Description ◽  
Good Yielding ◽  
Moderately Resistant

AC Watson, a medium–early maturing oilseed flax (Linum usitatissimum L.) was released in 1997 by Agriculture and Agri-Food Canada, Research Centre, Morden, Manitoba. The cultivar has high oil quality, medium-large seed size, good lodging resistance, and with good yielding in both early and late seeding in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description

Prairie Blue flax

2004 ◽  
Vol 84 (3) ◽  
pp. 801-803 ◽  
Author(s):  
S. D. Duguid ◽  
E. O. Kenaschuk ◽  
K. Y. Rashid
Keyword(s):  
Linum Usitatissimum ◽  
Oil Quality ◽  
High Yield ◽  
Research Station ◽  
Lodging Resistance ◽  
Linum Usitatissimum L ◽  
Melampsora Lini ◽  
Small Seed ◽  
Cultivar Description ◽  
Moderately Resistant

Prairie Blue, a medium- late maturing oilseed flax (Linum usitatissimum L.), was released in 2003 by Agriculture and Agri-Food Canada, Morden Research Station, Morden, Manitoba. This cultivar has high oil content, high oil quality, small seed size, very good lodging resistance and high yield in all soil zones of the prairies. It is immune to current North American races of rust [Melampsora lini (Ehrenb.) Desmaz], and moderately resistant to wilt caused by Fusarium oxysporum Schlecht. f. sp. lini (Bolley) Snyder & Hansen. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description

scholarly journals Transfer of label from 3H-glucose in Digitaria eriantha leaves to the rust fungus Puccinia digitariae Pole Evans.

1985 ◽  
Vol 33 (8) ◽  
pp. 809-812 ◽  
Author(s):  
M E Rey ◽  
H M Garnett
Keyword(s):  
Primary Infection ◽  
Secondary Infection ◽  
Rust Fungus ◽  
Infection Process ◽  
Bremia Lactucae ◽  
Infection Structures ◽  
Obligate Parasites ◽  
Digitaria Eriantha ◽  
Germ Tubes

Digitaria eriantha pentzii was fed 3H-glucose prior to inoculation with uredospores of Puccinia digitariae Pole Evans. Twenty-one hours after inoculation, uptake of label from 3H-glucose by the primary infection structures of P. digitariae was demonstrated employing autoradiography. These results indicate that an exchange of nutrients between host and pathogen occurs very early on in the infection process, during the formation of the primary infection structures. Despite contrary reports that obligate parasites receive no nutrition before establishment of haustoria, this study supports the work of Andrews (Can J Bot 53:1103, 1975), who demonstrated uptake of 3H-glucose label from lettuce cotyledons into the primary and secondary infection vesicles, appressoria, and germ tubes of Bremia lactucae.

Sign in / Sign up

Export Citation Format

Share Document