INFLUENCE OF NUTRIENTS ON PERITHECIAL PRODUCTION OF VENTURIA INAEQUALIS (CKE.) WINT.

1965 ◽  
Vol 43 (8) ◽  
pp. 959-965 ◽  
Author(s):  
R. G. Ross ◽  
S. A. Hamlin
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ammonium Sulfate ◽  
Narrow Range ◽  
Venturia Inaequalis ◽  
Synthetic Medium ◽  
Amino Acid Nitrogen ◽  
Low Concentrations ◽  
Nitrogen Concentrations ◽  
Ascospore Formation

Nitrates, urea, and amino acids were suitable sources of nitrogen, within a narrow range of concentrations, for perithecial development of Venturia inaequalis in a synthetic medium. Ammonium sulfate was not. The concentration of amino acid nitrogen required to inhibit the formation of perithecia varied with the amino acid. Zinc appeared to stimulate perithecial production. Amino acids differed in the nitrogen concentrations required to inhibit formation of perithecia. Perithecia developed in low concentrations of arabinose, glucose, sucrose, lactose, and maltose but did not develop in xylose, ribose, fructose, mannose, or galactose. The disaccharides were particularly suitable. Thiamine appeared to be necessary for ascospore formation.

THE EFFECT OF CERTAIN ELEMENTS, WITH EMPHASIS ON NITROGEN, ON THE PRODUCTION OF PERITHECIA OF VENTURIA INAEQUALIS (CKE.) WINT.

1961 ◽  
Vol 39 (4) ◽  
pp. 731-738 ◽  
Author(s):  
R. G. Ross
Keyword(s):  
Venturia Inaequalis ◽  
Synthetic Medium ◽  
Low Concentrations ◽  
Nitrate Salts ◽  
Nitrogen Concentrations ◽  
High Concentrations

Various elements were tested for their effect on the formation of perithecia of Venturia inaequalis. Apart from nitrogen the elements tested had little effect on the formation of perithecia except at relatively high concentrations. Perithecia did not form in media containing low concentrations of nitrogen and this was influenced by the source of nitrogen and by the cation present in nitrate salts. Perithecial initials developed in higher nitrogen concentrations than perithecia. Nitrogen added 9 weeks after mating of two lines of the fungus prevented the formation of perithecia.Results were difficult to reproduce in a potato-dextrose apple leaf decoction medium but reproducible results were obtained in a synthetic medium.

scholarly journals The Relation of the Composition of the Culture Medium to the Formation of Endospores by Aerobic Bacilli

1932 ◽  
Vol 32 (4) ◽  
pp. 535-543 ◽  
Author(s):  
H. L. A. Tarr
Keyword(s):  
Amino Acids ◽  
Culture Medium ◽  
Inorganic Salt ◽  
Synthetic Medium ◽  
Ammonium Phosphate ◽  
Spore Formation ◽  
Experimental Conditions ◽  
Nitrogenous Compounds ◽  
Low Concentrations ◽  
High Concentrations

1. Spore formation in eight typical members of the genusBacillushas been studied.2. Three of these strains, including one species ofB. anthracis, have been found to be practically asporogenous under the experimental conditions. In general the following statements hold good for the sporogenous races studied.3. Spore formation is almost, or entirely, inhibited by cultivation on media rich in amino acids, such as tryptic digests of casein or meat. Similar inhibition results following cultivation on a medium containing reasonably high concentrations of a mixture of amino acids and asparagine.4. When such media are suitably diluted with standard inorganic salt solutions the percentage of spores formed is greatly increased, and frequently at least 99 per cent. of spores are formed if the dilution is sufficiently high.5. When simple nitrogenous compounds such as amino acids are added to a dilute casein digest medium in which sporulation is almost complete, a definite decrease in the percentage of spores present is observed. Asparagine, which is probably readily assimilated, apparently completely hinders spore formation in most cases. Other amino acids do not exert so pronounced an effect, and ammonium phosphate does not appreciably inhibit the formation of spores.6. The fact that the addition of glycine suppresses growth markedly when it is added to a dilute casein digest medium, but does not appreciably hinder sporulation, suggests that the formation of spores is not due to any toxic effect of added compounds, or compounds already present in the medium.7. Sporulation is almost complete in a “synthetic” medium in which low concentrations of ammonium phosphate and sucrose represent the sources of nitrogen and carbon, respectively. However, frequent transfers in such a medium may inhibit spore formation partially or entirely in certain instances. This effect probably depends upon the enhanced ability of the culture in question to utilise sucrose as a source of carbon when cultivated constantly in its presence.8. It is concluded, from the above data, that endospore formation in aerobic bacilli bears an inverse relationship to the amount of available nutrient material present in the culture medium.I am indebted to Prof. Sir F. G. Hopkins and Miss M. Stephenson for their constant encouragement during the progress of this work. My thanks are due to Mr Pirie of this Department who kindly furnished me with several of the amino acids employed, and to Dr Miles of the Department of Pathology for his kindness in supplying me with certain of the cultures.

ESSENTIAL AMINO ACIDS IN MICROORGANISMS

1957 ◽  
Vol 3 (5) ◽  
pp. 721-728 ◽  
Author(s):  
F. Reusser ◽  
J. F. T. Spencer ◽  
H. R. Sallans
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Method ◽  
Essential Amino Acids ◽  
Cellular Protein ◽  
One Dimensional ◽  
Low Concentrations ◽  
Amino Acid Determination ◽  
High Concentrations ◽  
Acid Determination

The cells of 19 species of bacteria, actinomyces, and yeasts were analyzed for protein and essential amino acids. A rapid quantitative method for amino acid determination using one-dimensional paper chromatography was developed. The cellular protein from all species contained relatively high concentrations of lysine, somewhat lower concentrations of tryptophan and threonine, and very low concentrations of methionine. All of the 10 essential amino acids were found in each species tested, although individual differences in the relative and absolute amounts were observed.

Fluxes and membrane transport of amino acids in rat liver under different protein diets

1990 ◽  
Vol 259 (5) ◽  
pp. E614-E625 ◽  
Author(s):  
P. Fafournoux ◽  
C. Remesy ◽  
C. Demigne
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Aromatic Amino Acids ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Transport Systems ◽  
Low Concentrations ◽  
Significant Uptake

The aim of the present work was to evaluate in vivo the role of the transport step in hepatic amino acid metabolism. To vary hepatic utilization of amino acids, rats were adapted to diets containing various concentrations of casein (5, 15, and 60%). In rats fed 5 or 15% casein diets, Gln and Glu were released by the liver, and there was a significant uptake of Ala. Hepatic fluxes of amino acids increased considerably after adaptation to high-casein diet (up to 1.55 mumol.min-1.g liver-1 for Ala), because of the rise in afferent concentrations as well as enhanced uptake percentage (peaking at 60–75% for most glucogenic amino acids). Adaptation to a high-protein diet led to induction of not only system A but also of most of the other transport systems (Gly, anionic, T, y+, and to a lesser extent system N); only systems ASC and L were unchanged. The study of amino acid repartition between liver and plasma with different diets indicates that transport could modulate utilization of Ala, Ser, Thr, Gly, Gln, and Asp. For Arg and Asn, present in very low concentrations in liver under any condition, the transport step should be the major locus of control of their metabolism. For amino acids chiefly transported by nonconcentrative systems, such as aromatic amino acids, cellular metabolism could also be limited by the transport process. In conclusion, during adaptation to a high-protein diet, there is apparently a coordinated adaptation of amino acid transport and of their intracellular metabolism. For some amino acids, induction of catabolic enzymes seems greater than that of transport, so that the transport step may play an important role in control of metabolic fluxes. For example, concentration of amino acids such as Thr may be markedly depressed in rats adapted to a high-protein diet.

Components in brain heart infusion selective for chromogenic variants of Staphylococcus aureus

1970 ◽  
Vol 16 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Joseph T. Parisi ◽  
Michael P. Kiley
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Chromatographic Analysis ◽  
Synthetic Medium ◽  
Brain Heart Infusion ◽  
Large Numbers ◽  
Complete Synthetic Medium ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Large numbers of chromogenic variants were isolated from cultures of a parent strain of Staphylococcus aureus growing in the dialysate but not in the residue of brain heart infusion (Difco). Gas–liquid chromatographic analysis of the dialysate detected 18 amino acids in this medium. Large numbers of chromogenic variants also were isolated from 13 of 18 synthetic media deficient in a single amino acid but not in the complete synthetic medium containing all 18 amino acids. Gas–liquid chromatographic analysis detected marked quantitative differences in the amino acid metabolites present in a complete synthetic medium and the synthetic medium deficient in arginine after growth for 12 days. The data suggest that differences in the amino acid metabolism of the parent and chromogenic variants could account for the population changes observed in brain heart infusion.

scholarly journals On the Nutrition and Metabolism of Zooplankton II. The relationship between the marine copepod Calanus helgolandicus and particulate material in Plymouth sea water, in terms of amino acid composition

1963 ◽  
Vol 43 (2) ◽  
pp. 495-511 ◽  
Author(s):  
C. B. Cowey ◽  
E. D. S. Corner
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Total Nitrogen ◽  
Skeletonema Costatum ◽  
Particulate Material ◽  
Amino Acid Nitrogen ◽  
Calanus Helgolandicus

The amino acid composition of adult female Calanus helgolandicus Claus and particulate material at station L4 has been analysed from March to December. The average concentration of amino acid nitrogen in Calanus was 7·7% of the dry body weight and accounted for 83% of the total nitrogen. The average level of amino acid nitrogen in the particulate material was 11·9 μg/1. and accounted for 54% of the total nitrogen.The relative quantities of amino acids in Calanus remained remarkably constant throughout the year. The relative quantities of amino acids in particulate material were more variable.The rate of respiration of Calanus measured at 8°C varied from 31 μl. O2/mg. dry body weight/day in winter (December–February) to 79 μl. O2/mg dry body weight/day in summer (April–August).Starving winter Calanus lost 1·8% and starving summer Calanus 2·1% of their dry body weight as amino acids each day. In order to replenish its daily losses of amino acids winter Calanus must sustain a filtering rate of about 30 ml./animal/day: the corresponding value in summer is greater than 50 ml./animal/day.The amino acid composition of Skeletonema costatum is so close to that of the particulate material in the sea, that, as far as amino acids are concerned, Calanus would gain no nutritional advantage by selecting the diatom in preference to the amino acid containing fraction of particulate material as a whole.

scholarly journals Carbohydrate content of insoluble elastins prepared from adult bovine and calf ligamentum nuchae and tropoelastin isolated from copper-deficient procine aorta

1971 ◽  
Vol 121 (2) ◽  
pp. 197-202 ◽  
Author(s):  
M. E. Grant ◽  
F. S. Steven ◽  
D. S. Jackson ◽  
L. B. Sandberg
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Carbohydrate Content ◽  
Amino Sugars ◽  
Collagenase Digestion ◽  
Low Concentrations ◽  
Neutral Sugars ◽  
High Concentrations ◽  
Edta Extraction

1. Insoluble elastin has been prepared by several different methods from adult bovine and calf ligamentum nuchae. Highly purified tropoelastin has been prepared from copper-deficient porcine aorta. 2. Amino acid analyses indicated that all preparations, except that obtained from calf ligamentum nuchae by using an EDTA extraction followed by collagenase digestion (preparation E6), were typical of pure elastin having high concentrations of hydrophobic and low concentrations of hydrophilic amino acids. Preparation E6 was found to contain approx. 40% collagen. 3. The determination and composition of the carbohydrates associated with these preparations is reported. With the exception of preparation E6, the insoluble elastins contained only trace amounts of neutral sugars (0.13–0.35%, w/w) and amino sugars (0.01–0.06%, w/w). The porcine tropoelastin contained virtually no carbohydrate. 4. The results suggest that carbohydrate analyses can yield valuable information about the purity of elastin preparations.

UTILIZATION OF PARENTERALLY ADMINISTERED NITROGEN BY INFANTS RECEIVING SUBOPTIMAL CALORIES

PEDIATRICS ◽  
1959 ◽  
Vol 23 (4) ◽  
pp. 690-698
Author(s):  
Philip L. Calcagno ◽  
Mitchell I. Rubin ◽  
P. K. Mukherji
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Amino Acid ◽  
Nitrogen Balance ◽  
Protein Hydrolysate ◽  
Caloric Intake ◽  
Parenteral Administration ◽  
Amino Acid Nitrogen ◽  
Negative Balance ◽  
Protein Starvation

The parenteral administration of 100 mg of amino-acid nitrogen/kg of body weight/ day given within 4 hours to infants who are underfed (40 cal/kg of body weight/day) and protein-starved can change the nitrogen balance from negative to positive or greatly reduce the degree of negative balance. The retained nitrogen is not degraded to urea or excreted as amino acids in the urine in the after-period. It is postulated that the retained amino-acid nitrogen is utilized for synthesis of tissue. Even when the total caloric intake is suboptimal, the administration of a protein hydrolysate during prolonged protein starvation in infants who are unable to ingest protein seems desirable.

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