BRANCH CANKER AND DIEBACK OF QUERCUS PRINUS L. CAUSED BY A SPECIES OF BOTRYODIPLODIA

1965 ◽  
Vol 43 (6) ◽  
pp. 731-737 ◽  
Author(s):  
Robert A. Schmidt ◽  
Charles L. Fergus
Keyword(s):  
Petri Dish ◽  
Scale Insect ◽  
Early Summer ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
Canker Disease ◽  
Quercus Prinus ◽  
Foliar Blight ◽  
Branch Dieback ◽  
Branch Canker

An extensive dieback and canker disease of chestnut oak (Quercus prinus L.) in Pennsylvania was proved to be caused by Botryodiplodia sp. Isolations from infected stems, branches, buds, and petioles, many of which displayed stromata of Botryodiplodia sp., yielded cultures of this fungus. Inoculations of chestnut oak seedlings and saplings with Botryodiplodia sp. induced symptoms identical with those observed in nature. The fungus was subsequently reisolated from the inoculated diseased tissues. Symptoms occurring in nature were foliar blight, branch dieback, and branch canker. Extensive foliar symptoms, which appeared in late spring and early summer, probably resulted from infections which took place late in the previous summer or fall. Frequent association of a scale insect, Asterolecanium sp., with the disease implicated it as a contributing factor in the disease cycle. Maximum radial growth of the fungus on cornmeal and Bacto yeast nitrogen base agar occurred at 20 and 25 °C, respectively. Light was necessary for the production of stromata and conidia in culture. Microtome sections of petri dish cultures and diseased twigs showed that the fruiting structures of the pathogen were uni- or multi-locule pycnidial stromata. The stromata averaged 400–500 μ. in cross section and contained conidia which at maturity were brown, one-septate, and measured 13 × 27 μ.

scholarly journals Metabolic Engineering of Non-carotenoid-Producing Yeast Yarrowia lipolytica for the Biosynthesis of Zeaxanthin

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuxiao Xie ◽  
Shulin Chen ◽  
Xiaochao Xiong
Keyword(s):  
Yarrowia Lipolytica ◽  
Fold Increase ◽  
Genetically Engineered ◽  
Cell Factory ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
Yeast Extract Peptone Dextrose ◽  
Dry Cell Weight ◽  
A Cell ◽  
Β Carotene

Zeaxanthin is vital to human health; thus, its production has received much attention, and it is also an essential precursor for the biosynthesis of other critical carotenoids such as astaxanthin and crocetin. Yarrowia lipolytica is one of the most intensively studied non-conventional yeasts and has been genetically engineered as a cell factory to produce carotenoids such as lycopene and β-carotene. However, zeaxanthin production by Y. lipolytica has not been well investigated. To fill this gap, β-carotene biosynthesis pathway has been first constructed in this study by the expression of genes, including crtE, crtB, crtI, and carRP. Three crtZ genes encoding β-carotene hydroxylase from different organisms were individually introduced into β-carotene-producing Y. lipolytica to evaluate their performance for producing zeaxanthin. The expression of crtZ from the bacterium Pantoea ananatis (formerly Erwinia uredovora, Eu-crtZ) resulted in the highest zeaxanthin titer and content on the basis of dry cell weight (DCW). After verifying the function of Eu-crtZ for producing zeaxanthin, the high-copy-number integration into the ribosomal DNA of Y. lipolytica led to a 4.02-fold increase in the titer of zeaxanthin and a 721% increase in the content of zeaxanthin. The highest zeaxanthin titer achieved 21.98 ± 1.80 mg/L by the strain grown on a yeast extract peptone dextrose (YPD)–rich medium. In contrast, the highest content of DCW reached 3.20 ± 0.11 mg/g using a synthetic yeast nitrogen base (YNB) medium to culture the cells. Over 18.0 g/L of citric acid was detected in the supernatant of the YPD medium at the end of cultivation. Furthermore, the zeaxanthin-producing strains still accumulated a large amount of lycopene and β-carotene. The results demonstrated the potential of a cell factory for zeaxanthin biosynthesis and opened up an avenue to engineer this host for the overproduction of carotenoids.

scholarly journals Correlation of Fluconazole MICs with Clinical Outcome in Cryptococcal Infection

2000 ◽  
Vol 44 (6) ◽  
pp. 1544-1548 ◽  
Author(s):  
A. I. Aller ◽  
E. Martin-Mazuelos ◽  
F. Lozano ◽  
J. Gomez-Mateos ◽  
L. Steele-Moore ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Maintenance Therapy ◽  
National Committee ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
Cryptococcal Infection ◽  
Microdilution Method ◽  
Cryptococcal Disease ◽  
Fluconazole Therapy

ABSTRACT We have correlated the in vitro results of testing the susceptibility of Cryptococcus neoformans to fluconazole with the clinical outcome after fluconazole maintenance therapy in patients with AIDS-associated cryptococcal disease. A total of 28 isolates of C. neoformans from 25 patients (24 AIDS patients) were tested. The MICs were determined by the broth microdilution technique by following the modified guidelines described in National Committee for Clinical Standards (NCCLS) document M27-A, e.g., use of yeast nitrogen base medium and a final inoculum of 104 CFU/ml. The fluconazole MIC at which 50% of isolates are inhibited (MIC50) and MIC90, obtained spectrophotometrically after 48 h of incubation, were 4 and 16 μg/ml, respectively. Of the 25 patients studied, 4 died of active cryptococcal disease and 2 died of other causes. Therapeutic failure was observed in five patients who were infected with isolates for which fluconazole MICs were ≥16 μg/ml. Four of these patients had previously had oropharyngeal candidiasis (OPC); three had previously had episodes of cryptococcal infection, and all five treatment failure patients had high cryptococcal antigen titers in either serum or cerebrospinal fluid (titers, >1:4,000). Although 14 of the 18 patients who responded to fluconazole therapy had previously had OPC infections, they each had only a single episode of cryptococcal infection. It appears that the clinical outcome after fluconazole maintenance therapy may be better when the infecting C. neoformans strain is inhibited by lower concentrations of fluconazole for eradication (MICs, <16 μg/ml) than when the patients are infected with strains that require higher fluconazole concentrations (MICs, ≥16 μg/ml). These findings also suggest that the MICs determined by the modified NCCLS microdilution method can be potential predictors of the clinical response to fluconazole therapy and may aid in the identification of patients who will not respond to fluconazole therapy.

scholarly journals Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

2009 ◽  
Vol 75 (18) ◽  
pp. 5938-5942 ◽  
Author(s):  
Yumei Li ◽  
Lili Lu ◽  
Hongmei Wang ◽  
Xiaodong Xu ◽  
Min Xiao
Keyword(s):  
Cell Surface ◽  
Surface Engineering ◽  
Yeast Cells ◽  
Penicillium Expansum ◽  
Yeast Nitrogen Base ◽  
Gene Encoding ◽  
Nitrogen Base ◽  
Reading Frame ◽  
Acid Protein ◽  
Casamino Acids

ABSTRACT A novel gene encoding transglycosylating β-galactosidase (BGase) was cloned from Penicillium expansum F3. The sequence contained a 3,036-bp open reading frame encoding a 1,011-amino-acid protein. This gene was subsequently expressed on the cell surface of Saccharomyces cerevisiae EBY-100 by galactose induction. The BGase-anchored yeast could directly utilize lactose to produce galactooligosaccharide (GOS), as well as the by-products glucose and a small quantity of galactose. The glucose was consumed by the yeast, and the galactose was used for BGase expression, thus greatly facilitating GOS synthesis. The GOS yield reached 43.64% when the recombinant yeast was cultivated in yeast nitrogen base-Casamino Acids medium containing 100 g/liter initial lactose at 25°C for 5 days. The yeast cells were harvested and recycled for the next batch of GOS synthesis. During sequential operations, both oligosaccharide synthesis and BGase expression were maintained at high levels with GOS yields of over 40%, and approximately 8 U/ml of BGase was detected in each batch.

scholarly journals EFFECT OF NIGELLA SATIVA SEED EXTRACT ON THE VIABILITY OF CANDIDA GLABRATA

2019 ◽  
pp. 84-87
Author(s):  
FARAH DIBA ◽  
RATNA FARIDA ◽  
SRI REDJEKI
Keyword(s):  
Candida Glabrata ◽  
Nigella Sativa ◽  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Yeast Nitrogen Base ◽  
Nitrogen Base ◽  
Common Opportunistic Infection ◽  
Yeast Nitrogen Base Medium

Objective: Candidiasis is a common opportunistic infection of the oral cavity caused by a yeast-like fungus called Candida. Candida glabrata is thesecond most frequently isolated species from this condition, after Candida albicans. This study aimed to evaluate the effect of Nigella sativa (blackcumin), known to possess antifungal properties, on the viability of C. glabrata.Methods: C. glabrata was added to a 96-microwell plate that was coated with artificial saliva and exposed to various concentrations (6.25%, 12.5%,25%, and 50%) of N. sativa seed extract; amphotericin B (250 mg/mL) was used as the positive control and 200 μL of yeast nitrogen base medium asthe negative control. The viability percentage of C. glabrata was determined by MTT assay.Results: The results showed that the viability values of C. glabrata were lower after exposure to the N. sativa seed extract when compared with thenegative control.Conclusion: The viability of Candida glabrata was decreased with increasing concentrations of the extract.

scholarly journals Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species

2020 ◽  
Vol 1 ◽  
Author(s):  
Thaís Pereira de Mello ◽  
Marta Helena Branquinha ◽  
André Luis Souza dos Santos
Keyword(s):  
Biofilm Formation ◽  
Culture Media ◽  
Yeast Nitrogen Base ◽  
Human Pathogens ◽  
Nitrogen Base ◽  
Opportunistic Fungi ◽  
Abiotic Surfaces ◽  
Resistance Patterns ◽  
Almost All ◽  
Glucose Supplementation

Abstract Scedosporium and Lomentospora species are ubiquitous saprophytic filamentous fungi that emerged as human pathogens with impressive multidrug-resistance profile. The ability to form biofilm over several biotic and abiotic surfaces is one of the characteristics that contributes to their resistance patterns against almost all currently available antifungals. Herein, we have demonstrated that Scedosporium apiospermum, Scedosporium minutisporum, Scedosporium aurantiacum and Lomentospora prolificans were able to form biofilm, in similar amounts, when conidial cells were incubated in a polystyrene substrate containing Sabouraud medium supplemented or not with different concentrations (2%, 5% and 10%) of glucose, fructose, sucrose and lactose. Likewise, the glucose supplementation of culture media primarily composed of amino acids (SCFM, synthetic cystic fibrosis medium) and salts (YNB, yeast nitrogen base) did not modulate the biofilm formation of Scedosporium/Lomentospora species. Collectively, the present data reinforce the ability of these opportunistic fungi to colonize and to build biofilm structures under different environmental conditions.

scholarly journals Botryosphaeriaceae Species Associated with Avocado Branch Cankers in California

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1465-1473 ◽  
Author(s):  
Virginia McDonald ◽  
Akif Eskalen
Keyword(s):  
Internal Transcribed Spacer ◽  
Management Strategies ◽  
Internal Transcribed Spacer Region ◽  
Canker Disease ◽  
The Family ◽  
Fusicoccum Aesculi ◽  
Botryosphaeriaceae Species ◽  
Β Tubulin ◽  
Branch Canker ◽  
Future Management

Members of the family Botryosphaeriaceae cause branch cankers and dieback on California avocado trees. More intensive pruning, a practice associated with high-density planting that is becoming more common in the California avocado industry, may increase the occurrence of branch canker. This study was undertaken to identify and characterize the Botryosphaeriaceae spp. involved in the branch canker disease complex in order to develop future management strategies. From 2008 to 2009, branch cankers were sampled from four or five trees from each of eight avocado groves in five California counties. Six Botryosphaeriaceae spp. were identified based on morphology as well as phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) and a partial sequence of the β-tubulin gene. These six species included Neofusicoccum australe, N. luteum, N. parvum, an unknown Neofusicoccum sp., Fusicoccum aesculi, and Dothiorella iberica. Members of the Botryosphaeriaceae were isolated from all avocado-growing regions sampled in California; however, incidence and distribution of species varied. This report is the first description of the isolation of D. iberica from avocado branch cankers in California.

scholarly journals Mathematical Model on Branch Canker Disease in Sylhet, Bangladesh

2017 ◽  
Vol 13 (01) ◽  
pp. 80-87
Author(s):  
Rahman, S.M.S ◽  
Islam, M.A ◽  
Shahrear, P. ◽  
Islam, M.S.
Keyword(s):  
Mathematical Model ◽  
Canker Disease ◽  
Branch Canker

scholarly journals First Report of Botryosphaeria obtusa as Causal Agent of Olive Tree Branch Dieback in Tunisia

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 905-905 ◽  
Author(s):  
M. Chattaoui ◽  
A. Rhouma ◽  
M. Msallem ◽  
M. Pérez ◽  
J. Moral ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Olive Tree ◽  
Fruit Rot ◽  
Fluorescent Light ◽  
Internal Transcribed Spacer Region ◽  
Canker Disease ◽  
First Report ◽  
Botryosphaeria Obtusa ◽  
Olive Trees ◽  
Branch Dieback

A branch dieback of olive trees (Olea europaea L. cv. Manzanilla de Sevilla) was observed in 2010 in an orchard (50 ha), located in the Testour region of northern Tunisia. More than 50% of trees were severely damaged by the disease. Symptomatic trees presented dead branches and wilted leaves, which remained attached to the shoots, and the affected tissues appeared abnormally dark compared with the inner bark of healthy branches. Numerous pycnidia were observed on the surface of the infected branches. For diagnosis, symptomatic stems were collected and small pieces of discolored tissues were excised from lesion margins, surface sterilized in 0.5% sodium hypochlorite for 1 min, rinsed and dried on sterilized filter paper, then placed on acidified Difco potato dextrose agar plates (APDA; 2.5 ml of 25% lactic acid per liter). Plates were incubated at 25°C for 4 to 5 days, and hyphal tips from developing fungal colonies were transferred to PDA and placed under fluorescent light (12 h/day). A fastgrowing, pycnidia-producing fungus was consistently isolated, with conidia exuding onto the agar surface of 10-day-old cultures. On the basis of colony characteristics, isolates were identified as Botryosphaeria obtusa (3). Conidia were large, dark brown, aseptate, rounded at both ends or truncate at base, and 25 to 26.8 × 10.5 to 12.03 μm. Pathogenicity tests were performed on detached stems of cv. Manzanilla by 7-mm diameter mycelial plugs cut from actively growing cultures of the fungus. Stems (30 cm length) were cleaned, surface sterilized with sodium hypochlorite (0.25% for 2 min), and wounded with a sterilized scalpel. Mycelial disks were placed over wounds and wrapped with Parafilm to prevent desiccation. Control stems were mock inoculated with sterile agar plugs. Inoculated and control stems were placed in polyethylene boxes and incubated at 25°C. After 45 days, inoculated stems developed brown discoloration, and small dark pycnidia appeared on stem surfaces. Controls remained healthy. Koch's postulates were verified by isolating the fungus from symptomatic stems. To confirm the identification, DNA of one isolate was extracted and the fungal primers ITS1 and ITS4 (4) were used to amplify the internal transcribed spacer region of rDNA. Purified amplicons were sequenced and a BLAST search of the GenBank database revealed 99% homology with B. obtusa isolate HO166525.1. The anamorph of the fungus, Diplodia seriata, has been recognized as the cause of fruit rot of olive (1) and branch canker or dieback (2). To our knowledge, this is the first report of a canker disease of olive trees caused by B. obtusa in Tunisia. References: (1) J. Moral et al. Plant Dis. 92:311, 2008. (2) J. Moral et al. Phytopathology 100:1340, 2010. (3) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990.

scholarly journals Seasonal disease: Foamy bark canker of Citrus maxima in the delta region of Tamil Nadu

2021 ◽  
Vol 16 (1) ◽  
pp. 7-14
Author(s):  
G. Venkatesan ◽  
P.S. Sharavanan
Keyword(s):  
Tamil Nadu ◽  
Infected Plant ◽  
Fungal Species ◽  
Delta Region ◽  
Canker Disease ◽  
Decay Branch ◽  
Short Period ◽  
Infected Area ◽  
Citrus Maxima ◽  
Branch Dieback

The Citrus maxima, commonly called pummelo, are a Rutaceae family. The Canker disease recently had an issue on Citrus species in the Delta region of Tamil Nadu. This disease is appeared by foamy oozes from the bark. The infected plant dies slowly in a short period. This study was identified the microorganism causes of foamy disease from bark and infected area. Totally 19 fungi were isolated. Among these 16 fungi were isolated from uninfected bark, 3 fungal species belonging to Ascomycetes, 2 fungal species belonging to Coelomycetes, and 10 species be classed Hyphomycetes and one sterile form, though 11 fungal species were isolated from infected bark foamy ooze, eight Hyphomycetes, one Oomycete, and two sterile forms were isolated. The RPO statistical analysis resulted, the bark fungi have been separated a group fungus from foamy fungi such a few fungi as the Fusarium, Phytophthora, and yeast have isolated in the foam. Also, the Jaccard’s similarity showed 42.105% and dissimilar among to the bark and foamy fungus. The plant was decay, branch dieback and tree death may induce by fungi also that the Canker disease on Citrus may be caused by Phytophthora fungal species.

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