ANALYSIS OF THE HISTOCHEMICAL LOCALIZATION OF PEROXIDASE RELATED TO THE DIFFERENTIATION OF PLANT TISSUES

1959 ◽  
Vol 37 (3) ◽  
pp. 449-458 ◽  
Author(s):  
D. S. Van Fleet

Peroxidase is detectible in all tissues but is most reactive, in the basophilic cells of the histogens. Oxidation of applied phenols and aminophenols by peroxidase produces quinones and quinonediimines that are adsorbed by nucleic acids and other basophilic substances in the formative centers of primordia. Localized reactions for peroxidase occur in the axils of leaf primordia prior to bud formation and on the surface of apical meristems in a spiral pattern marking the points for the future development of leaf primordia. Peroxidase is detectible in advance of or accompanying cell division and declines after the division phase; decline of peroxidase at the end of the division phase is related to the increase of phenols, naphthols, and phenolases. Peroxidase declines in all tissues except the phloem; a continuous peroxidase system in the phloem connects primordia with adult tissue. The hypothesis is offered that the cellular units of the phloem peroxidase constitute a continuous system between primordia and adult tissue and is functional in catalyzing the reduction of hydrogen acceptors essential to cell division and the initiation of primordia.

2005 ◽  
Vol 130 (6) ◽  
pp. 793-798
Author(s):  
Miki Nakata ◽  
Nobuo Sugiyama ◽  
Tanachai Pankasemsuk

The structure and developmental patterns of inflorescence of longan (Dimocarpus longan Lour.) were studied microscopically and by the naked eye. In inflorescence of longan, compound dichasia are arranged on three to four orders of monopodial axes without the formation of terminal flowers, indicating that longan inflorescence is pleiothyrse; cymose partial inflorescences are arranged on more than two monopodial axes. Most of the monopodial axes had differentiated by the end of November just before the cool season. The first sign of inflorescence formation was the appearance of bract primordia at apical meristems of the preformed monopodial axes, with lateral axes preceding the main axes. Dichasia were formed in the axils of bract primordia, and the formation of bracts and dichasia continued. Bract appearance can be detected by the naked eye 1 week after microscopically detected bract appearance. Shoots with intermediate characteristics between the inflorescence and the vegetative shoots were formed; dichasia were formed on the lateral axes, but not on the main axes in intermediate shoots. These results suggest that apical meristems on the terminal shoot produce monopodial axes, together with foliage leaf primordia, before floral induction, but produce bract primordia and compound dichasia, which are composed of sympodial axes, after floral induction.


1978 ◽  
Vol 8 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Shelagh M. Baxter ◽  
Melvin G. R. Cannell

The top whorl of branches on each year's leader on mature P. sitchensis (Bong.) Carr. had more needles and longer needle internodes than branches lower down on the leader. All branches developed as buds for similar periods of time from April to October, but top branch buds developed larger apical meristems which generated needle primordia more rapidly than lower branch buds. In mid-August, top branch buds produced at least 13 primordia per day compared with only 5 per day in basal buds. In the next year, when these preformed buds extended, top branches produced about one and a half times as many cells per needle internode as basal branches, as judged by observing the pith. In both years, cell division was the important growth process affected by 'dominance' mechanisms between branch buds.


1962 ◽  
Vol s3-103 (63) ◽  
pp. 359-367
Author(s):  
JOAN M. WHITTEN

Changes occurring in the connective-tissue elements in the pupae of some Diptera Cyclorrhapha are described. The larva and the adult are characterized by an organically continuous system of investing membranes. The pupa is, on the whole, characterized by the absence of investing membranes. Early in the pupa most of the larval membranes are destroyed. In some cases the associated cells also disappear; in others the cells remain. Disappearance is not simultaneous throughout an organ; usually the anteriormost segments of the organ disappear first. The adult membranes are formed in the late pupa, after the processes of growth, cell-division, and differentiation are completed. Fusion of membranes would appear to result from co-ordinated secretion, and be a delicately timed process. It is suggested that the larval membranes are similarly produced in the embryo, at the end of differentiation and organ formation, and that in the case of both larva and adult, the invested cells are concerned with the secretion of, and the destruction of, the investing membranes.


Weed Science ◽  
1972 ◽  
Vol 20 (3) ◽  
pp. 233-237 ◽  
Author(s):  
J. F. Stritzke ◽  
E. J. Peters

Microscopic examination of central and soft offset bulbs of wild garlic(Allium vinealeL.) at senescence of the parent plants in May and June revealed embryonic plants with numerous root primordia and four or five shoot primordia. Hardshell bulbs and aerial bulblets contained only one or two root primordia and three leaf primordia. The embryonic plants of central, soft offset, and hardshell bulbs elongated slowly during the after-ripening period. Rapid cell division, cell elongation, and initiation of new leaves took place after termination of the after-ripening period in all but the dormant hardshell bulbs. In November, new hardshell bulbs could be seen at the base of plants developed from central and soft offset bulbs.


1986 ◽  
Vol 64 (11) ◽  
pp. 2662-2668 ◽  
Author(s):  
E. K. Merrill

The early developmental stages of simple and compound leaves of green ash (50–400 μm long) were used to relate cell division activity (mitotic index) to developing leaf form and histological differentiation. Densely cytoplasmic cells within cross-sectioned leaf primordia have higher mitotic indices than protodermal cells and other internal cells that are more vacuolate. Among densely cytoplasmic cells mitotic indices decrease from the primordial leaf margin toward the procambium. Ground meristem cells within three to five cell widths of the primordial margin had the highest mitotic indices. Actual cell counts indicate that densely cytoplasmic cells increase in number in areas of leaf blade or leaflet initiation more than do vacuolate cells or protodermal cells. It is proposed that marginal meristems defined by spatial and histological criteria are important in producing new cells that are the basis for the generation of simple and compound leaf forms.


Planta ◽  
1979 ◽  
Vol 146 (1) ◽  
pp. 71-74 ◽  
Author(s):  
D. S. Letham ◽  
R. E. Summons ◽  
C. W. Parker ◽  
J. K. MacLeod
Keyword(s):  

1951 ◽  
Vol 2 (1) ◽  
pp. 73-89 ◽  
Author(s):  
Maurice Ogur ◽  
Ralph O. Erickson ◽  
Gloria U. Rosen ◽  
Katharine B. Sax ◽  
Constance Holden

Tetrahedron ◽  
1967 ◽  
Vol 23 (1) ◽  
pp. 479-486 ◽  
Author(s):  
D.S. Letham ◽  
J.S. Shannon ◽  
I.R.C. McDonald
Keyword(s):  

1955 ◽  
Vol 1 (1) ◽  
pp. 17-28 ◽  
Author(s):  
David P. Bloch ◽  
Gabriel C. Godman

1. The fast green stain of Alfert and Geschwind for nuclear basic protein is shown to obey the Beer-Lambert laws when used on purified histone. Interference from acid substances other than nucleic acids as a possible source of error is indicated. 2. Use of this technique after a modified Feulgen stain enables determination of relative amounts of desoxyribonucleic acid and histone in the same individual cells. 3. DNA and histone are shown to have the same distribution in formalin-fixed nuclei. 4. The syntheses of DNA and histone proceed simultaneously resulting in the doubling of both these substances prior to cell division. 5. The standard error for histone values is greater than that for DNA; however, the source of this variability is not known.


Planta ◽  
1967 ◽  
Vol 74 (3) ◽  
pp. 228-242 ◽  
Author(s):  
D. S. Letham
Keyword(s):  

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