CYTOTAXONOMIC STUDIES ON RUMEX SECTION AXILLARES
The present investigation covers only the North American species of Rumex subgenus Rumex (Lapathum) section Axillares. A review was made of all the previous taxonomical and cytological work done in this group. All the species of the section Axillares were morphologically studied and their natural distribution plotted on maps. The distributional data were derived from about 3000 herbarium specimens gathered from various herbaria in the United States and Canada. Chromosome numbers of 17 taxa of the section Axillares were determined. The three subsections were found to comprise a polyploid series, the monotypic subsection Venosi with 2n = 40, R. verticillatus of the subsection Verticillati with 2n = 60, and all but two of the species studied in the subsection Salicifolii with 2n = 20; the two exceptions were R. mexicanus and R. utahensis, both with 2n = 40. Meiosis was studied in the species of the subsection Salicifolii only. Both the diploid and the tetraploid species showed essentially normal meiotic behavior. Pollen fertility was also very high in all the species. A number of intra- and inter-specific hybridizations were made between the taxa in the subsection Salicifolii, involving both the diploid and the tetraploid species. The resulting diploid (diploid × diploid), triploid (diploid × tetraploid), and tetraploid (tetraploid × tetraploid) F1 hybrids were both morphologically and cytologically studied. Morphologically almost all the diploid and tetraploid hybrids were intermediate between the two parents. The triploid hybrids showed more similarity to the tetraploid species involved in the cross, Meiosis in all the diploid hybrids was essentially normal with 10 bivalents. However, there was a significant decrease in chiasma frequency as compared with the parent species. This was postulated to be due to cryptic structural hybridity. There were three different categories of pollen fertility, ranging from low to very high, which were not correlated with any meiotic irregularities. All the diploid hybrids were fertile and set abundant seeds. Meiosis in the triploid hybrids was very irregular with mostly 10 bivalents and 10 univalents at metaphase I. Anaphases were also irregular, resulting in numerous micronuclei. All the triploid hybrids were sterile and set only a few undeveloped seeds. Meiosis in the tetraploid hybrid studied was also irregular with mostly 9 bivalents and 22 univalents. It was thus postulated that both the tetraploid species are allotetraploids with one genome in common between them and that this genome is essentially the same one shared by the diploid species so far cytologically studied. The tetraploid hybrids were completely sterile and did not set any seed. From the cytogenetic data, the diploid species studied were shown to be very closely interrelated. However, no taxonomic revision was undertaken owing to lack of more complete data involving all the species comprising the group.