COMPARATIVE STUDIES OF NORTH AMERICAN AND EUROPEAN CULTURES OF THE ROOT ROT FUNGUS, FOMES ANNOSUS (FR.) COOKE

1955 ◽  
Vol 33 (5) ◽  
pp. 416-428 ◽  
Author(s):  
D. E. Etheridge

Cultures of Fames annosus originating in Europe could not be distinguished from those originating in North America either by colony appearance, growth rate, pH optimum, or cellulolytic activity. Three growth rate types on 2.5% malt agar were recognized and these are ascribed to individual variation rather than to host or geographical influences. Successive subculturing produced variants that fell into three growth classes. Half of the isolates displayed spontaneous, but reversible, changes in growth rate and colony appearance during subculturing and this is discussed from the standpoint of genetical and environmental influences. Cultures displaying different morphological characteristics and linear-growth rates differed little metabolically; each had a similar pH optimum ranging from 4.6 to 5.5, and each proved capable of altering the initial acidity of the medium to a reaction which was more suitable for growth. Two cultures were characterized by double pH optima at 4.6 and 5.5. Cultures having different linear-growth rates produced about the same dry-weight of mycelium on a cellulose substrate in a semisynthetic nutrient solution. On the basis of a statistical analysis of cellulose utilization by representative isolates it was impossible to distinguish between North American and European cultures.

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1316-1316 ◽  
Author(s):  
M. M. Díaz Arias ◽  
G. P. Munkvold ◽  
L. F. Leandro

Fusarium spp. are widespread soilborne pathogens that cause important soybean diseases such as damping-off, root rot, Fusarium wilt, and sudden death syndrome. At least 12 species of Fusarium, including F. proliferatum, have been associated with soybean roots, but their relative aggressiveness as root rot pathogens is not known and pathogenicity has not been established for all reported species (2). In collaboration with 12 Iowa State University extension specialists, soybean roots were arbitrarily sampled from three fields in each of 98 Iowa counties from 2007 to 2009. Ten plants were collected from each field at V2-V3 and R3-R4 growth stages (2). Typical symptoms of Fusarium root rot (2) were observed. Symptomatic and asymptomatic root pieces were superficially sterilized in 0.5% NaOCl for 2 min, rinsed three times in sterile distilled water, and placed onto a Fusarium selective medium. Fusarium colonies were transferred to carnation leaf agar (CLA) and potato dextrose agar and later identified to species based on cultural and morphological characteristics. Of 1,230 Fusarium isolates identified, 50 were recognized as F. proliferatum based on morphological characteristics (3). F. proliferatum isolates produced abundant, aerial, white mycelium and a violet-to-dark purple pigmentation characteristic of Fusarium section Liseola. On CLA, microconidia were abundant, single celled, oval, and in chains on monophialides and polyphialides (3). Species identity was confirmed for two isolates by sequencing of the elongation factor (EF1-α) gene using the ef1 and ef2 primers (1). Identities of the resulting sequences (~680 bp) were confirmed by BLAST analysis and the FUSARIUM-ID database. Analysis resulted in a 99% match for five accessions of F. proliferatum (e.g., FD01389 and FD01858). To complete Koch's postulates, four F. proliferatum isolates were tested for pathogenicity on soybean in a greenhouse. Soybean seeds of cv. AG2306 were planted in cones (150 ml) in autoclaved soil infested with each isolate; Fusarium inoculum was applied by mixing an infested cornmeal/sand mix with soil prior to planting (4). Noninoculated control plants were grown in autoclaved soil amended with a sterile cornmeal/sand mix. Soil temperature was maintained at 18 ± 1°C by placing cones in water baths. The experiment was a completely randomized design with five replicates (single plant in a cone) per isolate and was repeated three times. Root rot severity (visually scored on a percentage scale), shoot dry weight, and root dry weight were assessed at the V3 soybean growth stage. All F. proliferatum isolates tested were pathogenic. Plants inoculated with these isolates were significantly different from the control plants in root rot severity (P = 0.001) and shoot (P = 0.023) and root (P = 0.013) dry weight. Infected plants showed dark brown lesions in the root system as well as decay of the entire taproot. F. proliferatum was reisolated from symptomatic root tissue of infected plants but not from similar tissues of control plants. To our knowledge, this is the first report of F. proliferatum causing root rot on soybean in the United States. References: (1) D. M. Geiser et al. Eur. J. Plant Pathol. 110:473, 2004. (2) G. L. Hartman et al. Compendium of Soybean Diseases. 4th ed. The American Phytopathologic Society, St. Paul, MN, 1999. (3) J. F. Leslie and B. A. Summerell. The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, UK, 2006. (4) G. P. Munkvold and J. K. O'Mara. Plant Dis. 86:143, 2002.


1979 ◽  
Vol 36 (12) ◽  
pp. 1497-1502 ◽  
Author(s):  
L. J. Buckley

The protein, DNA, and RNA content of larvae maintained at 1.0 plankter/mL increased at the rates of 9.3, 9.9, and 9.8% per day, respectively, for the 5 wk after hatching. Protein reserves of larvae held at 0 or 0.2 plankters/mL were depleted by 45 and 35%, respectively, prior to death 12–13 d after hatching. Starved larvae had similar protein concentrations (percent of dry weight), lower RNA concentrations, and higher DNA concentrations than fed larvae. Larvae held at higher plankton densities had higher RNA–DNA ratios and faster growth rates than larvae held at lower plankton densities. The RNA–DNA ratio was significantly correlated (P < 0.01) with the protein growth rate. The RNA–DNA ratio appears to be a useful index of nutritional status in larval Atlantic cod (Gadus morhua) and may be useful for determining if cod larvae were in a period of rapid or slow growth at the time of capture. Key words: RNA–DNA ratio, starvation, protein, nucleic acids, growth, larval fish, Atlantic cod


Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Sydney Olson ◽  
Marniker Wijesinha ◽  
Annalise Panthofer ◽  
William Blackwelder ◽  
Gilbert R Upchurch ◽  
...  

Objective: Small abdominal aortic aneurysms (AAAs) have a low risk of rupture. Intervention is indicated when diameters exceed established thresholds. This study assessed the growth rates and patterns of AAAs over 2 years as documented on serial CT scans from the Non-Invasive Treatment of AAA Clinical Trial. Methods: 254 patients, 35 females with baseline AAA maximum transverse diameter (MTD) between 3.5-4.5 cm and 219 males with baseline MTD 3.5-5.0 cm, were included in this study. Linear regressions and segmental growth rates were used to model growth rates and patterns. Results: The yearly growth rates of AAA MTDs had a median of 0.17 cm/yr and mean of 0.19 cm/yr ± 0.14 (Figure 1). 10% of AAA displayed minimal to no growth (< 0.05 cm/yr), 62% low growth (0.05-0.25 cm/yr), 28% high growth (> 0.25 cm/yr). Baseline AAA diameter accounted for only 5.4% of growth rate variance (P<0.001, R 2 0.05). Most AAAs displayed linear growth (70%); large variations in interval growth rates occurred infrequently (3% staccato growth, 4% exponential growth); a minority of subjects’ growth patterns were not clearly classifiable (11% indeterminate-not growing, 12% indeterminate-growing) (Figure 2). No patients with baseline MTD < 4.25 cm exceeded sex-specific repair thresholds (males 0 / 92, [95% CI, 0.00-0.06]; females 0 / 25 [95% CI, 0.00-0.25]) in the course of follow-up for as long as two years. Conclusions: The majority of small AAAs exhibit linear growth; large intra-patient growth rate variations were infrequently observed over 2 years. AAA < 4.25 cm can be followed with a CT scan in 2 years with little chance of exceeding interventional MTD thresholds of 5.5 cm for men.


2018 ◽  
Vol 96 (11) ◽  
pp. 1255-1263 ◽  
Author(s):  
Justin Smith ◽  
Shawna Karpovich ◽  
Greg A. Breed ◽  
Diane M. O’Brien

We investigated whether inferences about harbor seal (Phoca vitulina Linnaeus, 1758) whisker shed status and growth rates based on whisker morphology could improve dietary time series from whisker isotopic profiles. We identified morphometric characteristics of Alaskan harbor seal whiskers, including a smooth root section (SRS) and a bumpy section. The SRS was longer for spring versus fall-collected whiskers and matched the length of fully grown, shed whiskers. Thus, SRS length can differentiate whisker shed and growth status, and we used it to determine the sequence of whisker shedding by cohort in summer-captured harbor seals. The mean interbump length (IBL) correlated with whisker length, potentially providing a proxy for whisker growth rate. We compared carbon isotope ratios (expressed as δ13C values) along the three longest whiskers from 10 harbor seals, using two approaches for converting whisker position to date: (1) a standard growth rate constant based on captive studies and (2) individually adjusted growth rates based on whisker morphology. Intraindividual patterns of whisker δ13C values became more synchronous when expressed by deposition date rather than by position; however, adjusting growth rates based on IBL did not improve synchrony further. These findings suggest that whisker morphology can contribute whisker growth rate and shedding information for dietary reconstruction.


1976 ◽  
Vol 86 (3) ◽  
pp. 603-608 ◽  
Author(s):  
T. J. Riggs ◽  
P. G. Gothard

SummaryGrains from ears of known anthesis time in seven spring barley cultivars were measured for dry weight and α-amylase activity at regular intervals during grain maturation. During the period 10–31 days after anthesis, dry weight increase of the grain was found to be substantially linear in all the cultivars. Comparisons between linear slopes fitted for this phase of growth were found to provide an objective means of comparing grain growth rates in different cultivars.α-Amylase activity per grain reached a peak in all except one cultivar at between 10 and 16 days after anthesis but declined rapidly during the linear phase of grain growth. α-Amylase activity per gram grain dry weight decreased exponentially during this period and transformation of the data to logarithms allowed a substantially linear fit to be made. Slopes for each of the cultivars were compared.Differences were found between cultivars in grain growth rates, total α-amylase activity and rates of fall of α-amylase activity per gram for the period 10–31 days after anthesis. No relationship could be found between grain growth rate and either the absolute level of α-amylase activity achieved in the grain or the rate of fall in activity during development.


1999 ◽  
Vol 52 (1) ◽  
pp. 71 ◽  
Author(s):  
J. L. V. Lewandowski ◽  
R. M. Ellem

A 3-field model for collisional drift waves, in the ballooning representation, for a low-pressure stellarator plasma is presented. In particular, the effect of a finite radial mode number (≡ θk) is studied, and the linear growth rates for the fluctuating plasma density, electrostatic potential and electron temperature are computed numerically by solving the 3-field model as an initial-value problem. Numerical results for a 3-field period stellarator with low global magnetic shear are then presented. It is found that, in a system with small global magnetic shear, the case θk = 0 yields the fastest linear growth rate.


Author(s):  
Arnaud Pierens

Abstract Ringed structures have been observed in a variety of protoplanetary discs. Among the processes that might be able to generate such features, the Secular Gravitational Instability (SGI) is a possible candidate. It has also been proposed that the SGI might lead to the formation of planetesimals during the non-linear phase of the instability. In this context, we employ two-fluid hydrodynamical simulations with self-gravity to study the non-axisymmetric, non-linear evolution of ringed perturbations that grow under the action of the SGI. We find that the non-linear evolution outcome of the SGI depends mainly on the initial linear growth rate. For SGI growth rates smaller than typically σ ≳ 10−4 − 10−5Ω, dissipation resulting from dust feedback introduces a m = 1 spiral wave in the gas, even for Toomre gas stability parameters Qg &gt; 2 for which non-axisymmetric instabilities appear in a purely gaseous disc. This one-armed spiral subsequently traps dust particles until a dust-to-gas ratio ε ∼ 1 is achieved. For higher linear growth rates, the dust ring is found to undergo gravitational collapse until the bump in the surface density profile becomes strong enough to trigger the formation of dusty vortices through the Rossby Wave Instability (RWI). Enhancements in dust density resulting from this process are found to scale with the linear growth rate, and can be such that the dust density is higher than the Roche density, leading to the formation of bound clumps. Fragmentation of axisymmetric rings produced by the SGI might therefore appear as a possible process for the formation of planetesimals.


1981 ◽  
Vol 33 (3) ◽  
pp. 259-263 ◽  
Author(s):  
G. C. Priestley ◽  
M. L. Ryder

ABSTRACTThe linear growth rate of wool on three sites on each of six Wenslcydale × Blackface lambs was measured by wool fibre autoradiography following 11 injections of 35S-cystine. The first injection was given at 09.00 h, so that alternate 36h intervals between injections represented predominantly day (09.00 to 21.00h) or night (21.00 to 09.00 h). The overall mean growth rates on shoulder, mid-side and breech were 0·83, 0·96 and 0·95 mm/day. Differences between day and night growth rates were very small (proportionally<0·05) and inconsistent in 16 of the 18 samples; although breech samples from two sheep showed up to 0·14 faster growth in daytime, a circadian rhythm of wool growth seems unlikely on this evidence. Both linear wool growth rate and food intake increased steadily throughout the experiment.Mid-side wool growth rates in a second group of three crossbred longwool lambs averaged 0·79, 100 and 1·14 mm/day and were relatively stable over a period of several months.


1988 ◽  
Vol 34 (5) ◽  
pp. 694-696 ◽  
Author(s):  
Jaleed S. Ahmad ◽  
Ralph Baker

Two strains of Trichoderma harzianum and one each of T. koningii, T. polysporum, and T. viride were mutated for tolerance to the fungicide benomyl. Rhizosphere competence index of several mutants of each strain and species was determined by the rhizosphere competence assay. Most of the mutants and not their wild type parents were rhizosphere competent. When the strains and species were grown in Czapek–Dox broth for 6 days with cellulose as sole carbon source, the mutants produced significantly higher dry weight than their parent wild types. Neither the mutants nor the wild types produced biomass in glucose comparable to that in cellulose. Evidence indicates that Trichoderma spp. were induced by mutation to increase their linear growth rate and to become rhizosphere competent. Tolerance to benomyl does not seem to be a necessary attribute of rhizosphere competence.


2011 ◽  
Vol 52 (No. 4) ◽  
pp. 178-185 ◽  
Author(s):  
X. Liu ◽  
S.J. Herbert ◽  
K. Baath ◽  
A.M. Hashemi

Seeds are the primary sinks for photosynthates during reproductive growth. Variation in light intercepted during and after seed initiation has been found a major environmental determinant of soybean [Glycine max(L.) Merrill] seed size. We investigated the influence of light enrichment and shading on seed growth rate, effective filling, cotyledon cell number, cell volume and endogenousABA concentrations of cotyledons/testas during seed filling of soybean. Evans, an indeterminate Group 0 soybean, was subjected to light reduction and enrichment treatments from the beginning of pod formation until final harvest for two years inMassachusetts. Higher rates of seed growth, greater seed dry weight, and higher cotyledon cell number were all observed with light enrichment. There was a&nbsp;reduction in seed growth rate and cotyledon cell number, along with a significant lowering of endogenousABA levels in testa and cotyledon with shade. The level ofABAin cotyledon during seed development was significantly correlated with seed growth rates only under shade treatments. Both the growth rates and seed filling duration were influenced by variation in light interception by the soybean canopy. The effects of varying light treatment on seed size, within one genotype, were most likely due to the differences in seed growth rate and cotyledon cell number.


Sign in / Sign up

Export Citation Format

Share Document