Species delimitation, phylogenetic relationships, and two new species in the Cuscuta gracillima complex (Convolvulaceae)This paper is one of a selection of papers published in the Special Issue on Systematics Research.

Botany ◽  
2008 ◽  
Vol 86 (7) ◽  
pp. 670-681 ◽  
Author(s):  
Mihai Costea ◽  
Fiona Aiston ◽  
Saša Stefanović
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Species Delimitation ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Conservation Status ◽  
Special Issue ◽  
Dna Sequence Data ◽  
F Region ◽  
Selection Of

Basic morphology, scanning electron microscopy, and DNA sequence data from the plastid trnL–F region and the nuclear internal transcribed spacer (ITS) regions were used to delimit the species of a recently circumscribed clade of Cuscuta (Convolvulaceae) and to investigate their phylogenetic relationships. This clade comprises the Cuscuta gracillima complex from Mexico, Central and northern South America, a group which is characterized by inflorescences that appear to emerge directly from the host stem. Eight lineages are recognized, with two of them described here as new species: Cuscuta punana Costea & Stefanović, sp. nov. from Ecuador and Cuscuta vandevenderi Costea & Stefanović, sp. nov. from Mexico. Cuscuta colombiana Yunck is redefined to include Cuscuta aristeguietae Yunck., and Cuscuta deltoidea Yunck. is broadened to encompass Cuscuta serruloba Yunck. A taxonomic treatment with an identification key, descriptions, and illustrations is provided; the biogeography and conservation status of the eight species are also discussed.

Systematics of “horned” dodders: phylogenetic relationships, taxonomy, and two new species within the Cuscuta chapalana complex (Convolvulaceae)

Botany ◽  
2011 ◽  
Vol 89 (10) ◽  
pp. 715-730 ◽  
Author(s):  
Mihai Costea ◽  
Ignacio García Ruiz ◽  
Saša Stefanović
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Dna Sequence Data ◽  
26S Rdna ◽  
F Region ◽  
Two New Species ◽  
Northern South America ◽  
Nuclear Regions

The Cuscuta chapalana complex (Convolvulaceae) is a recently circumscribed clade within the subgenus Grammica that includes several little-known species from Mexico and Central and northern South America characterized by the presence of peculiar subapical multicelullar appendages on the corolla and often on the calyx lobes. Basic morphology, scanning electron microscopy, and DNA sequence data from the plastid trnL–F region and the 26S rDNA and internal transcribed spacer nuclear regions were used to investigate the phylogenetic relationships within the group and to test the species limits. Multicellular appendages are horn-like or tubular in shape and bear one or more stomata at their tips; hence the vernacular name “horned” dodders. Eight lineages were reconstructed; two of them, both from Mexico, were described as new species: Cuscuta bonafortunae Costea & I. García, sp. nov., and Cuscuta carnosa Costea & Stefanović, sp. nov. Cuscuta pringlei is redefined as a variety of Cuscuta strobilacea . A taxonomic treatment with an identification key, descriptions, and illustrations is provided.

Phylogenetic relationships in subtribe Poinae (Poaceae, Poeae) based on nuclear ITS and plastid trnT–trnL–trnF sequencesThis paper is one of a selection of papers published in the Special Issue on Systematics Research.

Botany ◽  
2008 ◽  
Vol 86 (8) ◽  
pp. 938-967 ◽  
Author(s):  
Lynn J. Gillespie ◽  
Robert J. Soreng ◽  
Roger D. Bull ◽  
Surrey W.L. Jacobs ◽  
Nancy F. Refulio-Rodriguez
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Close Relationships ◽  
Sequence Data ◽  
New Combination ◽  
Special Issue ◽  
Consensus Tree ◽  
Strict Consensus Tree ◽  
Nuclear Its ◽  
Selection Of

The worldwide temperate subtribe Poinae comprises the largest grass genus, Poa (500+ species), and multiple additional small genera. We explore generic boundaries and relationships among genera of Poinae using nuclear ribosomal internal transcribed spacer data (ITS) and plastid trnT–trnL–trnF (TLF) sequence data. ITS and TLF analyses are mostly congruent with regards to circumscription of genera, and with respect to relationships among Poinae genera, but tree structure is generally better supported among genera in the ITS strict consensus tree. ITS and TLF both support two main Poinae lineages: (i) Poa and (ii) a clade comprising all other sampled Poinae. Nine small genera were nested within the large Poa clade, including Aphanelytrum , Dissanthelium , and Tovarochloa , supporting inclusion of these as sections within Poa. In the second clade, three subclades support close relationships among Nicoraepoa , Hookerochloa , and Arctagrostis ; Arctophila and Dupontia ; and Apera , Bellardiochloa , and Ventenata . Genera of the related subtribes Alopecurinae, Cinninae, and Miliinae were mixed among or in part external to Poinae in different ways in ITS and TLF analyses, and only subtribe Puccinelliinae was strongly supported and monophyletic. ITS analyses supported placement of Catabrosella , Hyalopoa , and Paracolpodium in Puccinelliinae (no TLF data available). The position of Poa subgenus Arctopoa is incongruent between the two analyses: TLF data support inclusion within Poa and ITS data place it outside of Poa. Similarly, TLF data resolves the genus Aniselytron outside of Poa, whereas ITS data place it within Poa. Based on these results and a consideration of morphology, we recognize Arctopoa and Aniselytron as genera, probably of ancient hybrid origins. Nothogenus  × Duarctopoa is coined for Arctopoa × Dupontia, with a single nothospecies  × Duarctopoa labradorica . A new combination is provided for Poa subg. Sylvestres .

Revision of the genus Hannia (Teleostei, Terapontidae), with description of a new species, Hannia wintoni, from the Kimberley, Western Australia

Zootaxa ◽  
2020 ◽  
Vol 4869 (4) ◽  
pp. 562-586
Author(s):  
JAMES J. SHELLEY ◽  
AURÉLIEN DELAVAL ◽  
MATTHEW C. LE FEUVRE ◽  
TIM DEMPSTER ◽  
TARMO A. RAADIK ◽  
...  
Keyword(s):  
New Species ◽  
Western Australia ◽  
Sequence Data ◽  
Conservation Status ◽  
Original Description ◽  
Molecular Study ◽  
Meristic Characters ◽  
Dna Sequence Data ◽  
Freshwater Habitats ◽  
Allozyme Loci

The systematics of the genus Hannia Vari 1978, endemic to freshwater habitats of remote north-western Australia, is revised in light of recent collections in the region and a molecular study of the group that identified an undescribed candidate species. A new freshwater fish species (Hannia wintoni sp. nov) is described based on analysis of multiple nuclear genetic markers (53 allozyme loci), mitochondrial DNA sequence data (601 bp cytochrome b) and morphology (examination of a suite of 66 morphometric and meristic characters). Head profile, postorbital length, maximum length, preopercular spines and pectoral-fin rays are characters that best distinguish H. wintoni sp. nov from its only congener, H. greewayi. While the existing description of H. greenwayi is robust and accurate, we present a number of additional characters that enhance to the original description, based on type and fresh material. Information on the known distribution, habitats and conservation status of the two species is summarised. The new species is a narrow-range endemic. 

Pilea nonggangensis (Urticaceae), a new species from Guangxi, China

Phytotaxa ◽  
2017 ◽  
Vol 313 (1) ◽  
pp. 130 ◽  
Author(s):  
LONG-FEI FU ◽  
SHI-LIAN HUANG ◽  
ALEX K. MONRO ◽  
YING LIU ◽  
FANG WEN ◽  
...  
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Species Conservation ◽  
Its Region ◽  
Systematic Position ◽  
Molecular Evidence ◽  
Conservation Assessment ◽  
Dna Sequence Data ◽  
A New Species

Pilea nonggangensis Y. G. Wei, L. F. Fu & A. K. Monro, a new species from Guangxi, China is described and illustrated. The new species is morphologically most similar to P. basicordata from which it differs by being dioecious, having peltate leaf blades, membranous stipules, paired inflorescences and larger bracts. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new species and 20 local congeneric species are used to resolve the systematic position of the new species within Pilea. Despite vastly different morphology the molecular evidence suggests that P. nonggangensis is most closely related to P. pseudonotata. A Global Species Conservation Assessment classifies P. nonggangensis as Vulnerable (VU).

Culture experiments and DNA sequence data confirm the identity of Lobaria photomorphs

2003 ◽  
Vol 81 (3) ◽  
pp. 232-247 ◽  
Author(s):  
Soili Stenroos ◽  
Elfriede Stocker-Wörgötter ◽  
Isao Yoshimura ◽  
Leena Myllys ◽  
Arne Thell ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Green Alga ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Its Rdna ◽  
Dna Sequence Data

Lichens consist of a fungal component that associates with either a green alga or cynobacterium, resulting in diverse morphologies. Resynthesis experiments of isolated myco- and photobionts, as well as internal transcribed spacer (ITS) rDNA phylogenies, suggest that the notoriously dissimilar photomorphs of Lobaria fendleri (Tuck. ex Mont.) Lindau and Lobaria amplissima (Scop.) Forss. both have a single mycobiont, which can simultaneously lichenize two unrelated photobionts, a eukaryote and a prokaryote. In the phylogenetic analyses, the genera Sticta and Durietzia appear monophyletic; whereas Lobaria and Pseudocyphellaria are both nonmonophyletic. Furthermore, the phylogenetic relationships of Durietzia, Lobarina, and Sticta relative to Lobaria and Pseudocyphellaria are in need of re-evaluation.Key words: internal transcribed spacer (ITS), photosymbiodeme, phylogeny, lichen-forming fungi, Lobaria amplissima, Lobaria fendleri, Pseudocyphellaria, Sticta.

A New Species of Physeterostemon (Melastomataceae) from Bahia, Brazil, with Notes on the Phylogeny of the Genus

2009 ◽  
Vol 34 (2) ◽  
pp. 324-329 ◽  
Author(s):  
André M. Amorim ◽  
Renato Goldenberg ◽  
Fabián A. Michelangeli
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Dna Sequence ◽  
Atlantic Forest ◽  
Phylogenetic Relationships ◽  
Sequence Data ◽  
Sensu Stricto ◽  
The Other ◽  
Dna Sequence Data ◽  
A New Species

Physeterostemon thomasii is described from the southern Bahian Atlantic forest of Brazil. It differs from the other two species of the genus by the paleaceous trichomes on young stems and petioles, and by the strongly bullate/foveolate leaves. A phylogenetic analysis with all three species, based on rbcL and ndhF DNA sequence data, showed that Physeterostemon forms a monophyletic group, sister to Eriocnema fulva, and this clade is placed in a polytomy at the base of the tribe Miconieae sensu stricto. However, there is no resolution within Physeterostemon, thus the phylogenetic relationships of the three species cannot be established.

scholarly journals Four new species of Tremella (Tremellales, Basidiomycota) based on morphology and DNA sequence data

MycoKeys ◽  
2019 ◽  
Vol 47 ◽  
pp. 75-95 ◽  
Author(s):  
Ying Zhao ◽  
Xin-zhan Liu ◽  
Feng-yan Bai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
Morphological Characters ◽  
Dna Sequence Data ◽  
D2 Domain ◽  
High Species Diversity ◽  
Molecular Phylogenetic

In the present study, a total of 33 Tremella specimens in China were collected and examined using molecular phylogenetic analysis based on a combined sequence dataset of the nuc rDNA internal transcribed spacer (ITS) region and nuc 28S rDNA D1/D2 domain in conjunction with the morphological characters. Four new species, namely Tremellabasidiomaticola, T.cheejenii, T.erythrina, and T.salmonea, are newly described based on their distinct phylogenetic relationships and the comparison of morphological characters with known Tremella species. Our results indicate a high species diversity of Tremella waiting to be discovered.

Cardamine cubita (Brassicaceae), a new species from New Zealand with a remarkable reduction in floral parts

Phytotaxa ◽  
2013 ◽  
Vol 140 (1) ◽  
pp. 43
Author(s):  
Peter Brian Heenan ◽  
B. P. J. MOLLOY ◽  
R. D. SMISSEN
Keyword(s):  
New Species ◽  
New Zealand ◽  
Sequence Data ◽  
Conservation Status ◽  
Dna Sequence Data ◽  
Floral Characters ◽  
Central South ◽  
Remarkable Reduction ◽  
A New Species ◽  
Single Locality

A new species, Cardamine cubita, is named and described from the central South Island, New Zealand. DNA sequence data confirm that C. cubita belongs to a clade comprising C. hirsuta and C. oligosperma. This new species has a unique suite of reduced vegetative and floral characters. Cardamine cubita is known from a single locality, and is assigned a New Zealand conservation status of Nationally Critical.

Two new species of Crocicreas (Helotiaceae, Ascomycota) revealed by morphological and molecular data

Phytotaxa ◽  
2016 ◽  
Vol 272 (2) ◽  
pp. 149 ◽  
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Sequence Analyses ◽  
Dna Sequence Data ◽  
Ectal Excipulum ◽  
Two New Species ◽  
Morphological And Molecular Data

Two new species of Crocicreas are established based on morphological features and DNA sequence data. Crocicreas luteolum is distinct by combination of the following features: caulicolous, hymenium surface yellow, margin plane, ectal excipulum of textura prismatica, asci J+, 62−81 × 6.0−7.7 µm, and ascospores fusoid, biguttulate, 11−14 × 2.2−3.3 µm. Crocicreas pseudobambusae is featured by combination of bambusicolous, hymenium surface white to beige, margin plane, ectal excipulum of textura intricata, asci J+, 36−58 × 3.5−5.5 µm, and ascospores ellipsoid, eguttulate, 4.5−7.7 × 1.8−2.5 µm. Description, illustration and comparison with related fungi are provided for each species. Sequence analyses of the internal transcribed spacer of nuclear ribosomal DNA are used to confirm their generic positions.

A new Liopeltis Fitzinger, 1843 (Squamata: Colubridae) from Pulau Tioman, Peninsular Malaysia

Zootaxa ◽  
2020 ◽  
Vol 4766 (3) ◽  
pp. 472-484
Author(s):  
HANNAH E. SOM ◽  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
EVAN S. H. QUAH ◽  
RAFE M. BROWN ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Dna Sequence ◽  
Rare Species ◽  
Sequence Data ◽  
Peninsular Malaysia ◽  
Tropical Asia ◽  
Dna Sequence Data ◽  
A New Species ◽  
Lines Of Evidence

Liopeltis is a genus of poorly known, infrequently sampled species of colubrid snakes in tropical Asia. We collected a specimen of Liopeltis from Pulau Tioman, Peninsular Malaysia, that superficially resembled L. philippina, a rare species that is endemic to the Palawan Pleistocene Aggregate Island Complex, western Philippines. We analyzed morphological and mitochondrial DNA sequence data from the Pulau Tioman specimen and found distinct differences to L. philippina and all other congeners. On the basis of these corroborated lines of evidence, the Pulau Tioman specimen is described as a new species, L. tiomanica sp. nov. The new species occurs in sympatry with L. tricolor on Pulau Tioman, and our description of L. tiomanica sp. nov. brings the number of endemic amphibians and reptiles on Pulau Tioman to 12. 

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