Proteins responding to drought and high-temperature stress in Pinus armandii Franch

2007 ◽  
Vol 85 (10) ◽  
pp. 994-1001 ◽  
Author(s):  
Cai-Yun He ◽  
Jian-Guo Zhang ◽  
Ai-Guo Duan ◽  
Hong-Gang Sun ◽  
Li-Hua Fu ◽  
...  
Keyword(s):  
High Temperature ◽  
Molecular Mechanisms ◽  
High Temperature Stress ◽  
Ammonia Assimilation ◽  
Future Research ◽  
Plant Responses ◽  
Forest Plantation ◽  
Two Dimensional Gel Electrophoresis ◽  
Antioxidant Metabolism ◽  
Major Factors

Proteomic analysis provides a powerful method for studying plant responses to stress at the protein level. To study stress-responsive molecular mechanisms for Pinus armandii Franch, one of the most important forest plantation tree species in subalpine regions of Asia, we analyzed the response of 2-year-old P. armandii seedlings to drought and high temperature using two-dimensional gel electrophoresis. More than 550 reproducible needle proteins were detected in the controls and treatments, and the abundance of 27 proteins were found to change noticeably. We identified five proteins affected by drought stress and eight proteins affected by high temperature. These proteins are functionally quite diverse and are involved in photosynthesis, cell division and elongation, antioxidant metabolism, ammonia assimilation, growth and development, and protein folding. Our results provide fundamental data for future research on responses to drought and high temperature. As drought and high temperature are two major factors limiting the growth of subalpine forests during summer under recent global warming, this research may contribute to an understanding of the development of stress tolerance in trees.

scholarly journals WRKY Transcription Factor Response to High-Temperature Stress

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2211
Author(s):  
Zhuoya Cheng ◽  
Yuting Luan ◽  
Jiasong Meng ◽  
Jing Sun ◽  
Jun Tao ◽  
...  
Keyword(s):  
High Temperature ◽  
Plant Growth ◽  
Signaling Pathways ◽  
Temperature Stress ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
High Temperature Stress ◽  
Research Progress ◽  
Plant Responses ◽  
Gene Promoters

Plant growth and development are closely related to the environment, and high-temperature stress is an important environmental factor that affects these processes. WRKY transcription factors (TFs) play important roles in plant responses to high-temperature stress. WRKY TFs can bind to the W-box cis-acting elements of target gene promoters, thereby regulating the expression of multiple types of target genes and participating in multiple signaling pathways in plants. A number of studies have shown the important biological functions and working mechanisms of WRKY TFs in plant responses to high temperature. However, there are few reviews that summarize the research progress on this topic. To fully understand the role of WRKY TFs in the response to high temperature, this paper reviews the structure and regulatory mechanism of WRKY TFs, as well as the related signaling pathways that regulate plant growth under high-temperature stress, which have been described in recent years, and this paper provides references for the further exploration of the molecular mechanisms underlying plant tolerance to high temperature.

scholarly journals Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 687
Author(s):  
Chan Seop Ko ◽  
Jin-Baek Kim ◽  
Min Jeong Hong ◽  
Yong Weon Seo
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Two Dimensional Gel Electrophoresis ◽  
Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

scholarly journals Alterations of Endogenous Hormones, Antioxidant Metabolism, and Aquaporin Gene Expression in Relation to γ-Aminobutyric Acid-Regulated Thermotolerance in White Clover

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1099
Author(s):  
Hongyin Qi ◽  
Dingfan Kang ◽  
Weihang Zeng ◽  
Muhammad Jawad Hassan ◽  
Yan Peng ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Temperature ◽  
Temperature Stress ◽  
White Clover ◽  
High Temperature Stress ◽  
Aminobutyric Acid ◽  
Endogenous Hormones ◽  
Gaba Level ◽  
Antioxidant Metabolism ◽  
The Impact

Persistent high temperature decreases the yield and quality of crops, including many important herbs. White clover (Trifolium repens) is a perennial herb with high feeding and medicinal value, but is sensitive to temperatures above 30 °C. The present study was conducted to elucidate the impact of changes in endogenous γ-aminobutyric acid (GABA) level by exogenous GABA pretreatment on heat tolerance of white clover, associated with alterations in endogenous hormones, antioxidant metabolism, and aquaporin-related gene expression in root and leaf of white clover plants under high-temperature stress. Our results reveal that improvement in endogenous GABA level in leaf and root by GABA pretreatment could significantly alleviate the damage to white clover during high-temperature stress, as demonstrated by enhancements in cell membrane stability, photosynthetic capacity, and osmotic adjustment ability, as well as lower oxidative damage and chlorophyll loss. The GABA significantly enhanced gene expression and enzyme activities involved in antioxidant defense, including superoxide dismutase, catalase, peroxidase, and key enzymes of the ascorbic acid–glutathione cycle, thus reducing the accumulation of reactive oxygen species and the oxidative injury to membrane lipids and proteins. The GABA also increased endogenous indole-3-acetic acid content in roots and leaves and cytokinin content in leaves, associated with growth maintenance and reduced leaf senescence under heat stress. The GABA significantly upregulated the expression of PIP1-1 and PIP2-7 in leaves and the TIP2-1 expression in leaves and roots under high temperature, and also alleviated the heat-induced inhibition of PIP1-1, PIP2-2, TIP2-2, and NIP1-2 expression in roots, which could help to improve the water transportation and homeostasis from roots to leaves. In addition, the GABA-induced aquaporins expression and decline in endogenous abscisic acid level could improve the heat dissipation capacity through maintaining higher stomatal opening and transpiration in white clovers under high-temperature stress.

scholarly journals Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244198
Author(s):  
Xiaoxiao Qin ◽  
Panpan Li ◽  
Shaowei Lu ◽  
Yanchuan Sun ◽  
Lifeng Meng ◽  
...  
Keyword(s):  
High Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Molecular Mechanisms ◽  
Soluble Sugar ◽  
High Temperature Stress ◽  
Temperature Treatment ◽  
Sucrose Metabolism ◽  
High Temperature Treatment ◽  
Lactuca Sativa L

High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located on 1,766 phosphopeptides from 987 phosphoproteins were identified after high-temperature treatment,among which 217 phosphoproteins significantly changed their expression abundance (116 upregulated and 101 downregulated). Most phosphoproteins for which the abundance was altered were associated with the metabolic process, with the main molecular functions were catalytic activity and transporter activity. Regarding the functional pathway, starch and sucrose metabolism was the mainly enriched signaling pathways. Hence, high temperature influenced phosphoprotein activity, especially that associated with starch and sucrose metabolism. We suspected that the lettuce shorten its growth cycle and reduce vegetative growth owing to changes in the contents of starch and soluble sugar after high temperature stress, which then led to early bolting/flowering. These findings improve our understanding of the regulatory molecular mechanisms involved in lettuce bolting.

scholarly journals Plant Responses and Tolerance to High Temperature Stress: Role of Exogenous Phytoprotectants

2015 ◽  
pp. 385-435 ◽  
Author(s):  
Kamrun Nahar ◽  
Mirza Hasanuzzaman ◽  
Kamal Uddin Ahamed ◽  
Khalid Rehman Hakeem ◽  
Munir Ozturk ◽  
...  
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
High Temperature Stress ◽  
Plant Responses

CHANGES IN ANTIOXIDANT ISOZYMES AS A BIOMARKER FOR CHARACTERIZING HIGH TEMPERATURE STRESS TOLERANCE IN RICE (ORYZA SATIVA L.) SPIKELETS

2012 ◽  
Vol 49 (1) ◽  
pp. 53-73 ◽  
Author(s):  
SMRUTI DAS ◽  
P. KRISHNAN ◽  
MONALISA NAYAK ◽  
B. RAMAKRISHNAN
Keyword(s):  
High Temperature ◽  
Stress Tolerance ◽  
Temperature Stress ◽  
Oryza Sativa L ◽  
High Temperature Stress ◽  
Spikelet Sterility ◽  
Antioxidant Metabolism ◽  
Different Temperatures ◽  
Temperature Exposure

SUMMARYHigh temperature stress at flowering can adversely affect rice yield, largely due to failure of fertilization. Oxidative damage can be a major reason inducing spikelet sterility in rice. In the present study, the effect of high temperatures on antioxidant metabolism in rice spikelets was characterised using nine different genotypes. Exposure to different temperatures at flowering stage revealed significant differences among various antioxidant enzymes in spikelets, both quantitatively and qualitatively. Spikelets of susceptible genotypes withstood temperature stress of up to 35 °C, those of moderately tolerant between 35 °C and 38 °C and those of tolerant genotypes up to 40 °C. Presence or absence, and changes in the isozyme intensities were consistent with alterations in their activities. Superoxide dismutase (SOD) isozymes II and III were present after exposure at 30 °C and 35 °C, while SOD I appeared above 40°C. Intensities of catalase isozymes I and III and the only isozyme of ascorbate peroxidase altered, while the only isozyme of guaical peroxidase and two (III and IV) of the four isozymes of catechol peroxidase disappeared after high temperature exposure of 45 °C. Thus, this work provides an evidence of the role of antioxidant metabolism in spikelets under high temperature stress conditions. Hence, changes in antioxidant isozymes in rice spikelets can be used as a biomarker for characterizing high temperature stress tolerance in rice spikelets.

scholarly journals Physiological and Transcriptomic Analyses Reveal Exogenous Trehalose Is Involved in the Responses of Wheat Roots to High Temperature Stress

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2644
Author(s):  
Yin Luo ◽  
Yanyang Xie ◽  
Weiqiang Li ◽  
Maohuan Wei ◽  
Tian Dai ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Temperature ◽  
Temperature Stress ◽  
Gene Expression Regulation ◽  
High Temperature Stress ◽  
Physiological Data ◽  
Glutathione Metabolism ◽  
Plant Responses ◽  
Wheat Roots ◽  
Wheat Varieties

High temperature stress seriously limits the yield and quality of wheat. Trehalose, a non-reducing disaccharide, has been shown involved in regulating plant responses to a variety of environmental stresses. This study aimed to explore the molecular regulatory network of exogenous trehalose to improve wheat heat tolerance through RNA-sequencing technology and physiological determination. The physiological data and RNA-seq showed that trehalose reduced malondialdehyde content and relative conductivity in wheat roots, and affecting the phenylpropane biosynthesis, starch and sucrose metabolism, glutathione metabolism, and other pathways. Our results showed that exogenous trehalose alleviates the oxidative damage caused by high temperature, coordinating the effect of wheat on heat stress by re-encoding the overall gene expression, but two wheat varieties showed different responses to high temperature stress after trehalose pretreatment. This study preliminarily revealed the effect of trehalose on gene expression regulation of wheat roots under high temperature stress, which provided a reference for the study of trehalose.

scholarly journals A Systematic View Exploring the Role of Chloroplasts in Plant Abiotic Stress Responses

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Yo-Han Yoo ◽  
Woo-Jong Hong ◽  
Ki-Hong Jung
Keyword(s):  
Abiotic Stress ◽  
High Temperature ◽  
Stress Response ◽  
Temperature Stress ◽  
Stress Responses ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Close Association ◽  
High Temperature Stress ◽  
Growth And Yield

Chloroplasts are intracellular semiautonomous organelles central to photosynthesis and are essential for plant growth and yield. The significance of the function of chloroplast-related genes in response to climate change has not been well studied in crops. In the present study, the initial focus was on genes that were predicted to be located in the chloroplast genome in rice, a model crop plant, with genes either preferentially expressed in the leaf or ubiquitously expressed in all organs. The characteristics were analyzed by Gene Ontology (GO) enrichment and MapMan functional classification tools. It was then identified that 110 GO terms (45 for leaf expression and 65 for ubiquitous expression) and 1,695 genes mapped to MapMan overviews were strongly associated with chloroplasts. In particular, the MapMan cellular response overview revealed a close association between heat stress response and chloroplast-related genes in rice. Moreover, features of these genes in response to abiotic stress were analyzed using a large-scale publicly available transcript dataset. Consequently, the expression of 215 genes was found to be upregulated in response to high temperature stress. Conversely, genes that responded to other stresses were extremely limited. In other words, chloroplast-related genes were found to affect abiotic stress response mainly through high temperature response, with little effect on response to drought and salinity stress. These results suggest that genes involved in diurnal rhythm in the leaves participate in the reaction to recognize temperature changes in the environment. Furthermore, the predicted protein–protein interaction network analysis associated with high temperature stress is expected to provide a very important basis for the study of molecular mechanisms by which chloroplasts will respond to future climate changes.

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