Engineering nitrogen use efficiency with alanine aminotransferase

2007 ◽  
Vol 85 (3) ◽  
pp. 252-262 ◽  
Author(s):  
Allen G. Good ◽  
Susan J. Johnson ◽  
Mary De Pauw ◽  
Rebecka T. Carroll ◽  
Nic Savidov ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Seed Yield ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Fertilizer ◽  
Alanine Aminotransferase ◽  
Crop Productivity ◽  
Wild Type ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Use

Nitrogen (N) is the most important factor limiting crop productivity worldwide. The ability of plants to acquire N from applied fertilizers is one of the critical steps limiting the efficient use of nitrogen. To improve N use efficiency, genetically modified plants that overexpress alanine aminotransferase (AlaAT) were engineered by introducing a barley AlaAT cDNA driven by a canola root specific promoter (btg26). Compared with wild-type canola, transgenic plants had increased biomass and seed yield both in the laboratory and field under low N conditions, whereas no differences were observed under high N. The transgenics also had increased nitrate influx. These changes resulted in a 40% decrease in the amount of applied nitrogen fertilizer required under field conditions to achieve yields equivalent to wild-type plants.

scholarly journals Effect of sowing time and nitrogen fertilizer rates on growth, seed yield and nitrogen use efficiency of quinoa (Chenopodium quinoa Willd) in Ahvaz, Iran

2020 ◽  
Vol 21 (4) ◽  
pp. 354-367
Author(s):  
Srorlmolook Saeidi ◽  
Seyed Ataollah Siadat ◽  
Ali Moshatati ◽  
محمدرضا Moradi-Telavat ◽  
Niazali Sepahvand ◽  
...  
Keyword(s):  
Seed Yield ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Fertilizer ◽  
Chenopodium Quinoa ◽  
Sowing Time ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Fertilizer Rates

Physiological analysis of nitrogen-efficient rice overexpressing alanine aminotransferase under different N regimes

Botany ◽  
2013 ◽  
Vol 91 (12) ◽  
pp. 866-883 ◽  
Author(s):  
Perrin H. Beatty ◽  
Rebecka T. Carroll ◽  
Ashok K. Shrawat ◽  
David Guevara ◽  
Allen G. Good
Keyword(s):  
Transgenic Rice ◽  
Nitrogen Use Efficiency ◽  
Alanine Aminotransferase ◽  
Nitrogen Use ◽  
Physiological Analysis ◽  
Use Efficiency ◽  
Amino Acid Levels ◽  
Transcriptome Response ◽  
Low Nitrogen ◽  
N Use

Cereal crop plants have low nitrogen (N) use efficiency, taking up only 30% to 50% of the applied N fertilizers, with the rest having the potential for loss into the environment as N pollution. One way to address this problem is to improve the nitrogen use efficiency of cereal crops using a transgenic approach. We developed alanine aminotransferase overexpressing rice, and we have previously determined that this modification provided an improved nitrogen-use phenotype to the engineered plants. In this study, the transgenic rice were grown in low, medium, and high nitrogen supply, and morphology, plant N levels, enzymatic activity, metabolite levels, and transcriptome response in the roots and shoots at active and maximum tillering at each N level were measured. The transcriptome response was analysed further using MapMan and PageMan to view multiple comparisons. The transgenic rice plants showed improved nitrogen use efficiency at medium and high N supply, but with few significant changes to the amino acid levels or to the transcriptome. The transgenic plants grown in high N showed up-regulation of transcripts associated with photosynthesis, non-melavonate pathway secondary metabolites, protein degradation, and many unknown function transcripts.

Interaction of erect panicle genotype and nitrogen fertilizer application on the source-sink ratio and nitrogen use efficiency in rice

2022 ◽  
Vol 278 ◽  
pp. 108430
Author(s):  
Olusegun Idowu ◽  
Yuanzheng Wang ◽  
Koki Homma ◽  
Tetsuya Nakazaki ◽  
Zhengjin Xu ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Nitrogen Fertilizer ◽  
Fertilizer Application ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Source Sink

Identification of regulatory genes to improve nitrogen use efficiency

2014 ◽  
Vol 94 (6) ◽  
pp. 1009-1012 ◽  
Author(s):  
David R. Guevara ◽  
Yong-Mei Bi ◽  
Steven J. Rothstein
Keyword(s):  
Amino Acids ◽  
Transgenic Plants ◽  
Nitrogen Use Efficiency ◽  
Complex Traits ◽  
Regulatory Genes ◽  
Yield Potential ◽  
Environmental Damage ◽  
Nitrogen Use ◽  
Rice Plants ◽  
Use Efficiency

Guevara, D. R., Bi, Y.-M. and Rothstein, S. J. 2014. Identification of regulatory genes to improve nitrogen use efficiency. Can. J. Plant Sci. 94: 1009–1012. Crop production on soils containing sub-optimal levels of nitrogen (N) severely compromises yield potential. The development of crop varieties displaying high N use efficiency (NUE) is necessary in order to optimize N fertilizer use, and reduce the environmental damage caused by the current excessive application of N in agricultural areas. Genome-wide microarray analysis of rice plants grown under N-limiting environments was performed to identify NUE candidate genes. An early nodulin gene, OsENOD93-1, was strongly up-regulated during plant growth under low N. A constitutive Ubiquitin promoter was used to drive the expression of the OsENOD93-1 gene in transgenic plants to determine the importance of OsENOD93-1 for rice NUE. Transgenic rice plants over-expressing the OsENOD93-1 gene achieved ∼23% and 16% more yield and biomass, respectively, compared with wild-type plants when grown under N-limitation conditions. OsENOD93-1-OX transgenic plants accumulated a higher amount of total amino acids in the roots and xylem sap under N stress, suggesting that OsENOD93-1 plays a role in the transportation of amino acids. Taken together, we demonstrate that an effective way to identify NUE gene candidates involves both transcriptional profiling coupled with a transgenic validation approach to improve complex traits such as NUE in important crops.

scholarly journals Rice NIN-LIKE PROTEIN 4 is a master regulator of nitrogen use efficiency

2020 ◽  
Author(s):  
Jie Wu ◽  
Zi-Sheng Zhang ◽  
Jing-Qiu Xia ◽  
Alamin Alfatih ◽  
Ying Song ◽  
...  
Keyword(s):  
N Uptake ◽  
Crop Improvement ◽  
Field Trials ◽  
Growth And Yield ◽  
Wild Type ◽  
Inorganic N ◽  
Master Regulator ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Use

AbstractNitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavor in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the NIN-like protein OsNLP4 significantly improves the rice NUE and yield. Field trials consistently showed that loss-of-OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signaling genes by directly binding to the nitrate-responsive cis-element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 is a master regulator of NUE in rice and sheds light on crop NUE improvement.

scholarly journals Optimized split nitrogen fertilizer increase photosynthesis, grain yield, nitrogen use efficiency and water use efficiency under water-saving irrigation

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhen Zhang ◽  
Yongli Zhang ◽  
Yu Shi ◽  
Zhenwen Yu
Keyword(s):  
Winter Wheat ◽  
Grain Yield ◽  
Water Use Efficiency ◽  
Water Use ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Fertilizer ◽  
Water Saving ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Nitrogen Ratio

AbstractThis study aims to investigate optimization of the basal-top-dressing nitrogen ratio for improving winter wheat grain yield, nitrogen use efficiency, water use efficiency and physiological parameters under supplemental irrigation. A water-saving irrigation (SI) regime was established and sufficient irrigation (UI) was used as a control condition. The split-nitrogen regimes used were based on a identical total nitrogen application rate of 240 kg ha−1 but were split in four different proportions between sowing and the jointing stage; i.e. 10:0 (N1), 7:3 (N2), 5:5 (N3) and 3:7 (N4). Compared with the N1, N2 and N4 treatments, N3 treatment increased grain yield, nitrogen and water use efficiencies by 5.27–17.75%, 5.68–18.78% and 5.65–31.02%, respectively, in both years. The yield advantage obtained with the optimized split-nitrogen fertilizer application may be attributable to greater flag leaf photosynthetic capacity and grain-filling capacity. Furthermore, the N3 treatment maintained the highest nitrogen and water use efficiencies. Moreover, we observed that water use efficiency of SI compared with UI increased by 9.75% in 2016 and 10.79% in 2017, respectively. It can be concluded that SI along with a 5:5 basal-top-dressing nitrogen ratio should be considered as an optimal fertigation strategy for both high grain yield and efficiency in winter wheat.

Sign in / Sign up

Export Citation Format

Share Document