Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice

2006 ◽  
Vol 84 (8) ◽  
pp. 1196-1202 ◽  
Author(s):  
M. Farooq ◽  
S.M.A. Basra ◽  
M. Khalid ◽  
R. Tabassum ◽  
T. Mahmood

The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for rice nursery preparation), hydropriming for 48 h, osmohardening with KCl or CaCl2 (ψs –1.25 MPa solution) for 24 h, ascorbate priming (10 mg·L–1) for 48 h, and hardening for 24 h. Compared with controls, all priming treatments (except pregermination) reduced the time to start germination, improved the rate of germination and synchronization, and the length of shoot and root, seedling fresh and dry mass, number of secondary roots, the concentration of reducing sugars, and α-amylase activity, although the extent of these changes was different in seeds subjected to different treatments. These seed treatments resulted in higher germination that might be due to overcoming dormancy. Osmohardening with KCl was more effective than CaCl2 for these parameters. Nitrogen concentration remained unaffected in seedlings; however, Ca2+ concentrations in both seeds and seedlings were greater in seeds osmohardenerd with CaCl2 than with all other treatments, including the control. Seed priming enhanced K+ concentration in both seeds and seedlings, leading to improved α-amylase activity. There were positive correlations between seed K+ concentration and amylase activity, and the concentration of reducing sugars with amylase activity, seedling dry mass, or number of secondary roots. Osmohardening with KCl performed better than all other treatments including control. Priming improved the K+ balance that activates α-amylase, a basis for seed invigoration.

2018 ◽  
Vol 47 (4) ◽  
pp. 831-837
Author(s):  
D. Udhaya Nandhini ◽  
E. Somasundaram

Effects of nod factors (lipo chitooligosaccharide) on seedling growth of maize under salt stress have been studied. The study was framed with seed priming using lipo chitooligosaccharide (LCO) a nod factor @ 4 ml/kg of seeds and without priming on maize seedling growth which was exposed to different levels of salinity (0, 2, 4, 6, 8 and 10 dS/m). Salinity negatively influenced the seedling growth of maize. However nod factor treated maize seedlings had relatively higher germination percentage, root length, shoot length, reducing sugars and amylase activity. Correlation analysis revealed positive relationships between seedling growth parameters. Thus, seed priming with nod factor (4 ml/kg) improved the resistance to salinity at seedling stage. NaCl concentrations restricted amylase enzyme activity and reducing sugar content in the germinating seeds. Priming of nod factor/LCO mitigated the restricted effects of salinity on amylase activity and reducing sugars.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sheila Bigolin Teixeira ◽  
Stefânia Nunes Pires ◽  
Gabriele Espinel Ávila ◽  
Bruna Evelyn Paschoal Silva ◽  
Victoria Novo Schmitz ◽  
...  

AbstractRice is a crop that presents sensitivity to cold, especially in the germination phase, which leads to high economic losses. Alternative management forms are essential to increase tolerance to low temperatures, and seed priming represents a promising tool. The objective of this study was to investigate the priming effect of the aqueous extract of carrot roots on rice seeds to increase tolerance to low temperatures during germination. Seeds from cultivars BRS Querência (cold-susceptible) and Brilhante (cold-tolerant) were soaked for 24 h in concentrations of 0, 25, 50, and 100% carrot extract, sown on germitest paper and conditioned in BOD for 21 days at 15 °C. As a control, the seeds soaked in water were also germinated at 25 °C. They were evaluated for germination, first germination count, and germination speed index to calculate the stress indices: tolerance index, susceptibility index, and harmonic mean. They were also evaluated for the length and dry mass of shoot and root. The results showed that the rice seeds conditioning in carrot extract effectively reduces the damage caused by cold, significantly increasing the germination speed and the percentage of final germination and the growth evaluations, more expressive at 100% concentration. The stress indexes are efficient in estimating the tolerance of the cultivars and the effect of the different conditions in low-temperature conditions, highlighting the superiority of the Brilhante cultivar.


2021 ◽  
Vol 756 (1) ◽  
pp. 012047
Author(s):  
M P Anwar ◽  
R Jahan ◽  
M R Rahman ◽  
A K M M Islam ◽  
F M J Uddin

2009 ◽  
Vol 39 (8) ◽  
pp. 1600-1605 ◽  
Author(s):  
Jeffrey A. Lombardo ◽  
Brian C. McCarthy

Acorn weevils (Coleoptera: Curculionidae) are well-known predators of oak acorns in the eastern hardwood forest region of the United States. We examined the germination percentage of seeds, as well as the physical characteristics of seedlings, originating from both weevil-damaged and sound acorns of red oak ( Quercus rubra L.) to determine if weevil depredation impacts seedling vigor. Seeds were grown under greenhouse conditions for a period of 8 weeks. X-ray imaging was used as a nondestructive method for determining seed condition prior to germination. The combined data set showed a reduction in germination percentage from 86% for sound acorns to 26% for those damaged by weevils. Seven seedling metrics were evaluated for differences between seedlings originating from sound and those from weevil-damaged acorns. Of these metrics, the number of leaf flushes, total number of leaves, root collar diameter, shoot length, and dry mass were significantly (P < 0.05) different. Two variables, root length and shoot diameter, were not significantly different. Early allocation of resources to root establishment likely explains these findings. Because of reduced stem height and leaf number, seedlings from weevil-damaged acorns are likely to be less competitive in the forest understory than those from sound acorns.


2021 ◽  
Vol 43 ◽  
Author(s):  
Bruno Silva Guirra ◽  
Caio César Pereira Leal ◽  
Keylan Silva Guirra ◽  
Tatianne Raianne Costa Alves ◽  
Salvador Barros Torres ◽  
...  

Abstract: “Mofumbo” (Combretum leprosum Mart. (Combretaceae) is a neotropical species of wood, pharmacological, beekeeping and environmental importance. Thus, the objective was to evaluate the biochemical and physiological responses in C. leprosum seeds under different storage environments. The experiment was carried out in a factorial (2 x 7), with two storage conditions (natural and climate-controlled environments) and seven storage periods (0; 60; 120; 180; 240; 300 and 360 days), in four replications per treatment. At the beginning of storage and at 60-day intervals, the seeds were analyzed for water content, physiological potential (germination, germination speed index, root and shoot lengths and seedling dry mass) and biochemical (neutral lipids, lipid peroxidation, total and reducing sugars, total free amino acids, and starch). The results indicated that storing “mofumbo” seeds for 360 days reduced viability, being more pronounced after 180 days, regardless of the storage environment. “Mofumbo” seeds were stored in a natural environment for 180 days, resulting in less lipid degradation. After this period, the reduction was associated with the attack of reducing sugars on amino acids. Thus, it is concluded that “mofumbo” seeds must be stored in a natural environment for up to 180 days.


2017 ◽  
Vol 41 (2) ◽  
pp. 117-127 ◽  
Author(s):  
Thais Ramos da Silva ◽  
Jairo Osvaldo Cazetta ◽  
Samira Domingues Carlin ◽  
Bruna Robiati Telles

ABSTRACT Although there are evidences that the proper supply of mineral nutrients to plants relieves water stress, little is known on the approach of how the drought affects the absorption and accumulation of nutrients by distinct sugar cane genotypes, or in different parts of a same plant. Thus, the objective of this study was to determine the content and accumulation of N, P and K in the aerial part of plant from three genotypes of sugar cane, submitted to three water potentials in the soil, and check the relationship of these variables with the tolerance of plants to prolonged drought. In order to access this objective, an experiment under greenhouse conditions, comprised by a fatorial 3 × 3, in a randomized block design, and four replicates was carried out. After 90 days from treatment imposition, the plant transpiration rate, plant dry mass, concentration of N, P and K were determined in leaves and culms, as well as in total plant shoot were measured. It was found that tolerance to drought in sugar cane is related to higher levels of N and K in the leaves and stems, and larger accumulations of K and P in the plant shoot. There is high positive correlations among accumulation of N, P and K in the plant shoot and dry matter production by plants submitted to drought. There are intermediate correlations among plant transpiration and nutrient uptake by plants under drought. Plants of the genotype SP81-3250 are more tolerante to prolonged drought, than the RB855453 and IACSP95-5000 plants.


2001 ◽  
Vol 44 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Luiz Carlos de Oliveira Lima ◽  
Adimilson Bosco Chitarra ◽  
Maria Isabel F. Chitarra

Changes in amylase activity, starch and reducing and non-reducing sugars contents were monitored during ripening of mango fruits (Mangifera indica L.). The climateric raising in mango fruit is marked by an appreciable increase in the activity of amylase, reducing and non-reducing sugars contents and decrease in the starch content. The fruit affected with spongy tissue exhibited much lower amylase activity and reducing and non-reducing sugars, but exhibited much higher starch content during storage at 12 ± 2° C and 90 ± 5% RH for 28 days, when compared to healthy tissue of ‘Tommy Atkins’. Whether this is caused due to adverse effects on certain enzyme activities during ripening is not clearly known. These dates showed that carbohydrate metabolism is an important feature during ripening of mango.


1970 ◽  
Vol 16 (5) ◽  
pp. 390-395 ◽  
Author(s):  
John A Lott ◽  
Joan E Mercier

Abstract Serum or urine is incubated at 50°C with a substrate containing dextrin. The endogenous glucose and reducing sugars liberated from the substrate by amylase are separately determined on the AutoAnalyzer by use of alkaline potassium ferricyanide. Optimum conditions and limitations were evaluated. The method is compared to those of Henry and Chiamori (Somogyi) and Fishman and Doubilet. Normal values for serum and urine are presented.


1964 ◽  
Vol 47 (5) ◽  
pp. 823-826
Author(s):  
James F Eheart ◽  
Blanche S Mason

Abstract Determinations were made of total and reducing sugars, sucrose, dextrin, and starch content of dry fat-free solids of the "total diet" samples previously reported by Food and Drug Administration. Samples represented differences in season, location, time of purchase, and store at which purchased. Mean dry fat-free solids contents did not differ significantly. Purchase periods and locations caused extreme variability of the sucrose content of the diets. Factor- effect variations among the other carbohydrate constituents were not considered nutritionally important. Reducing sugars, sucrose, and starch gave positive correlations with total sugars, while negative correlations were obtained for total sugars with dextrin and for reducing sugars with sucrose.


2017 ◽  
Vol 30 (1) ◽  
pp. 149-154 ◽  
Author(s):  
LENNIS AFRAIRE RODRIGUES ◽  
◽  
CHARLINE ZARATIN ALVES ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
TIAGO ROQUE BENETOLI DA SILVA ◽  
...  

ABSTRACT The development of technologies to increase stand establishment efficiency and seed vigor, as well as to maximize profits, is of fundamental importance for the sustainability of agriculture. Products based on humic substances, such as humic acids, have been recently used in Brazilian agriculture to produce seedlings with high vigor, which positively influences the establishment of the initial stand. Thus, this study aimed to evaluate the effect of treatment of corn seeds with a commercial humic acid-based product, Humykos® (18% of humic acid content), on germination and seedling vigor. The treatments consisted of six doses (0, 100, 200, 300, 400, and 500 mL 100 kg-1 seed) of Humykos®, and the treated seeds were analyzed for germination and vigor (first count, emergence, emergence speed index, length, and dry mass of shoots and roots). Our results show that humic acid promotes greater seedling growth and increased shoot dry mass in corn; in addition, it has a positive influence on the emergence speed index up to a dose 158 mL 100 kg -1 seeds.


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