Elements regulating AGAMOUS expression are conserved between Arabidopsis thaliana, Brassica napus, and Linum usitatissimum

2003 ◽  
Vol 81 (6) ◽  
pp. 523-530 ◽  
Author(s):  
Jeffrey D Pylatuik ◽  
Rebecca H Cross ◽  
Arthur R Davis ◽  
Peta C Bonham-Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Linum Usitatissimum ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Close Relative ◽  
Cis Elements ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

To investigate the functional conservation of cis regulatory elements controlling AGAMOUS (AG) expression, we placed the promoter region of AG from Arabidopsis thaliana into a close relative, Brassica napus, and a distantly related species, Linum usitatissimum, and analyzed the subsequent expression patterns in each species. Spatially, the expression patterns in all three species were marginally similar, in that expression was confined primarily to the reproductive organs and nectarium. Within organs however, tissue-specific expression patterns were not conserved between species. Unlike Arabidopsis, the transgenic AG cis elements did not express in the ovules of B. napus and L. usitatissimum. Temporally, the pattern of AG cis-element expression in B. napus was identical to that of Arabidopsis during early development; however, the AG cis elements did not express at all during early flower development in L. usitatissimum. These results suggest that although regulatory factors controlling the generalized local expression of AG have been conserved between these species, those controlling temporal and tissue-specific expression have not.Key words: AGAMOUS, cis elements, regulation, Arabidopsis, Brassica napus, Linum usitatissimum.

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies

2021 ◽  
Vol 11 ◽  
Author(s):  
Voddu Suresh ◽  
Deepti Parida ◽  
Aliva P. Minz ◽  
Manisha Sethi ◽  
Bhabani S. Sahoo ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Golden Hamster ◽  
Expression Patterns ◽  
Mesocricetus Auratus ◽  
Syrian Golden Hamster ◽  
Surface Epithelium ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Specific Expression Pattern

The Syrian golden hamster (Mesocricetus auratus) has recently been demonstrated as a clinically relevant animal model for SARS-CoV-2 infection. However, lack of knowledge about the tissue-specific expression pattern of various proteins in these animals and the unavailability of reagents like antibodies against this species hampers these models’ optimal use. The major objective of our current study was to analyze the tissue-specific expression pattern of angiotensin-converting enzyme 2, a proven functional receptor for SARS-CoV-2 in different organs of the hamster. Using two different antibodies (MA5-32307 and AF933), we have conducted immunoblotting, immunohistochemistry, and immunofluorescence analysis to evaluate the ACE2 expression in different tissues of the hamster. Further, at the mRNA level, the expression of Ace2 in tissues was evaluated through RT-qPCR analysis. Both the antibodies detected expression of ACE2 in kidney, small intestine, tongue, and liver. Epithelium of proximal tubules of kidney and surface epithelium of ileum expresses a very high amount of this protein. Surprisingly, analysis of stained tissue sections showed no detectable expression of ACE2 in the lung or tracheal epithelial cells. Similarly, all parts of the large intestine were negative for ACE2 expression. Analysis of tissues from different age groups and sex didn’t show any obvious difference in ACE2 expression pattern or level. Together, our findings corroborate some of the earlier reports related to ACE2 expression patterns in human tissues and contradict others. We believe that this study’s findings have provided evidence that demands further investigation to understand the predominant respiratory pathology of SARS-CoV-2 infection and disease.

Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants

1990 ◽  
Vol 14 (4) ◽  
pp. 491-499 ◽  
Author(s):  
Oscar Vorst ◽  
Frans van Dam ◽  
Renske Oosterhoff-Teertstra ◽  
Sjef Smeekens ◽  
Peter Weisbeek
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Specific Expression ◽  
Tobacco Plants ◽  
Tissue Specific ◽  
Tissue Specific Expression

The Murine Genome Contains Two Functional Kininogen Genes Leading to Tissue Specific Expression of High Molecular Weight Kininogen (HK) and Expression of a Novel HK Splice Variant.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3979-3979
Author(s):  
Sergei Merkoulov ◽  
Anton A. Komar ◽  
Keith R. McCrae
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Expression Patterns ◽  
Endothelial Cell Proliferation ◽  
Head Orientation ◽  
High Molecular Weight Kininogen ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Exon 10

Abstract High molecular weight kininogen (HK) plays an important role in the assembly and activation of the kallikrein/kinin system. While the human genome contains only a single copy of the kininogen gene, three copies are present in the rat (one K-kininogen and two T-kininogen). Here, we report that the mouse genome contains two homologous kininogen genes (overall homology 91%), denoted mHK1 and mHK2. Both genes are located on chromosome 16 in a head-to-head orientation, and contain open reading frames. The size of intronic sequences between the 11 kininogen gene exons is similar (Figure). HK mRNA transcripts derived from the mHK1 and mHK2 genes differ slightly in size due to gaps of 33 and 18 nucleotides in exon 10 of mHK2. RT-PCR analysis of HK gene expression in adult and embryonic murine tissues revealed that HK mRNA was derived from mHK1 in liver, adrenal and embryo, but from mHK2 in kidney and lung. HK mRNA derived from both genes was present in testis, brain and muscle, though expression levels were low relative to those in other tissues. HK mRNA was not detected in ovary, bone marrow, heart or bladder. mHK1-derived HK mRNA was alternatively spliced, as demonstrated by the presence of an HK mRNA transcript encoding a novel HK1 isoform, ΔmD5, that lacked the portion of exon 10 encoding Thr400 - Asp582 of HK domains 5 and 6. Examination of the putative promoter regions of the two genes using the MatInspector Professional program (Genomatix) demonstrated distinct differences, perhaps explaining in part their tissue-specific expression patterns. Like domain 5 of human HK (hD5), domain 5 of murine HK (mD5), in which the histidine and lysine-rich C-terminal region of this domain previously shown to mediate the antiangiogenic activity of domain 5 is highly conserved, inhibited endothelial cell proliferation. While the function of each of the kininogen genes in the intact animal has yet to be defined, characterization of the two genes may provide new information concerning the role of high molecular weight kininogen in development, normal physiology, and pathological processes. Figure Figure

scholarly journals Tissue-Specific Expression Patterns of MicroRNA during Acute Graft-versus-Host Disease in the Rat

2016 ◽  
Vol 7 ◽  
Author(s):  
Dasaradha Jalapothu ◽  
Margherita Boieri ◽  
Rachel E. Crossland ◽  
Pranali Shah ◽  
Isha A. Butt ◽  
...  
Keyword(s):  
Graft Versus Host Disease ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Host Disease ◽  
Graft Versus Host ◽  
Tissue Specific Expression ◽  
Acute Graft

Tissue-specific expression of PPAR mRNAs in diabetic rats and divergent effects of cilostazol

2008 ◽  
Vol 86 (7) ◽  
pp. 465-471 ◽  
Author(s):  
Furong Wang ◽  
Ling Gao ◽  
Bendi Gong ◽  
Jianting Hu ◽  
Mei Li ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Renal Cortex ◽  
Expression Patterns ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Peroxisome Proliferator ◽  
Specific Expression ◽  
Camp Content ◽  
Tissue Specific ◽  
Tissue Specific Expression

Cilostazol and ligands of peroxisome proliferator-activated receptors (PPARs) have been effectively used to alleviate diabetic complications, but the common and tissue-specific expression patterns of PPARs in different tissues in diabetic patients and those treated with cilostazol have not been reported. Here, we aimed to assess the effects of diabetes and cilostazol on mRNA expression of PPARα and PPARγ in the aorta, renal cortex, and retina of diabetic rats treated with cilostazol for 8 weeks. PPARα mRNA expression showed uniform downregulation in all these tissues in diabetic rats, and this effect was reversed by cilostazol treatment. Surprisingly, PPARγ mRNA expression was reduced in the renal cortex and retina, yet increased in the aorta of diabetic rats, although cilostazol still reversed these changes. Interestingly, cilostazol, a well-known phosphodiesterase 3 inhibitor and cAMP elevator, augmented cAMP content only in the aorta, but showed no significant effects in the renal cortex of diabetic rats. In conclusion, mRNA expression of PPARs is tissue-specific in diabetes and may be differently affected by cilostazol, possibly because of its tissue-specific effects on cAMP content.

scholarly journals Human acyl-CoA:cholesterol acyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma

2006 ◽  
Vol 394 (3) ◽  
pp. 617-626 ◽  
Author(s):  
Bao-Liang Song ◽  
Can-Hua Wang ◽  
Xiao-Min Yao ◽  
Li Yang ◽  
Wen-Jing Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Transcription Factor ◽  
Molecular Mechanism ◽  
Luciferase Reporter ◽  
Hepatoma Cell Line ◽  
Cis Elements ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Cdx2 Expression

Humans express two ACAT (acyl-CoA:cholesterol acyltransferase) genes, ACAT1 and ACAT2. ACAT1 is ubiquitously expressed, whereas ACAT2 is primarily expressed in intestinal mucosa and plays an important role in intestinal cholesterol absorption. To investigate the molecular mechanism(s) responsible for the tissue-specific expression of ACAT2, we identified five cis-elements within the human ACAT2 promoter, four for the intestinal-specific transcription factor CDX2 (caudal type homeobox transcription factor 2), and one for the transcription factor HNF1α (hepatocyte nuclear factor 1α). Results of luciferase reporter and electrophoretic mobility shift assays show that CDX2 and HNF1α exert a synergistic effect, enhancing the ACAT2 promoter activity through binding to these cis-elements. In undifferentiated Caco-2 cells, the ACAT2 expression is increased when exogenous CDX2 and/or HNF1α are expressed by co-transfection. In differentiated Caco-2 cells, the ACAT2 expression significantly decreases when the endogenous CDX2 or HNF1α expression is suppressed by using RNAi (RNA interference) technology. The expression levels of CDX2, HNF1α, and ACAT2 are all greatly increased when the Caco-2 cells differentiate to become intestinal-like cells. These results provide a molecular mechanism for the tissue-specific expression of ACAT2 in intestine. In normal adult human liver, CDX2 expression is not detectable and the ACAT2 expression is very low. In the hepatoma cell line HepG2 the CDX2 expression is elevated, accounting for its elevated ACAT2 expression. A high percentage (seven of fourteen) of liver samples from patients affected with hepatocellular carcinoma exhibited elevated ACAT2 expression. Thus, the elevated ACAT2 expression may serve as a new biomarker for certain form(s) of hepatocellular carcinoma.

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