Identity and phylogenetic relationships of ophiostomatoid fungi associated with invasive and native Tetropium species (Coleoptera: Cerambycidae) in Atlantic Canada

2003 ◽  
Vol 81 (4) ◽  
pp. 316-329 ◽  
Author(s):  
Karin Jacobs ◽  
Keith A Seifert ◽  
Ken J Harrison ◽  
Thomas Kirisits
Keyword(s):  
Original Description ◽  
Picea Rubens ◽  
Atlantic Canada ◽  
Ophiostomatoid Fungi ◽  
Ophiostoma Piceae ◽  
Longhorn Beetle ◽  
Tetropium Fuscum ◽  
Eurasian Species ◽  
Molecular Characters ◽  
Isolated Species

In 1999, red spruce (Picea rubens Sarg.) trees in a small area of the Halifax Regional Municipality in Nova Scotia, Atlantic Canada, were found to be infested and killed by Tetropium fuscum (Fabr.) (Coleoptera: Cerambycidae), a Eurasian species of longhorn beetle. We isolated and identified the fungi associated with T. fuscum in Halifax and compared them with ophiostomatoid fungi associated with Tetropium spp. on spruce in Europe. Ophiostoma species associated with Tetropium cinnamopterum Kirby (indigenous to and transcontinental in Canada) were also compared with those associated with T. fuscum. Strains were identified based on morphological and molecular characters. Ophiostoma piceae (Münch) Syd. & P. Syd., Pesotum fragrans Mathiesen-Käärik, and Ophiostoma tetropii Mathiesen were the most frequently isolated species. Lack of type material for O. tetropii and discrepancies between the ex-type strain of O. tetropii and its original description prompted us to lectotypify this species and designate epitype material for it. The results suggest that O. tetropii was introduced with T. fuscum from Europe into Atlantic Canada.Key words: Ophiostoma tetropii, Tetropium fuscum, molecular phylogeny, invasive species.

Ophiostomatoid Fungi Associated with Cryphalus Piceae in Shandong province in eastern China

2021 ◽  
Author(s):  
Runlei Chang ◽  
Xiuyu Zhang ◽  
Hongli Si ◽  
Guoyan Zhao ◽  
Xiaowen Yuan ◽  
...  
Keyword(s):  
Bark Beetles ◽  
Novel Species ◽  
Eastern China ◽  
Fungal Species ◽  
Shandong Province ◽  
Morphological Data ◽  
Ophiostomatoid Fungi ◽  
Fungal Isolates ◽  
Study Sites ◽  
Isolated Species

Abstract Cryphalus piceae parasitizes various economically important conifers. Similar to other bark beetles, C. picea vectors an assortment of fungi and nematodes. Previously, several ophiostomatoid fungi were isolated from C. piceae in Poland and Japan. In the present study, we explored the diversity of ophiostomatoid fungi associated with C. piceae infesting pines in the Shandong Province of China. We isolated ophiostomatoid fungi from both galleries and beetles collected from our study sites. These fungal isolates were identified using both molecular and morphological data. Through this study, we recovered 176 isolates of ophiostomatoid fungi representing at least seven species. Ophiostoma ips was the most frequently isolated species. Analyses of molecular and morphological data indicated four of the ophiostomatoid fungal species recovered in this study were previously undescribed. Hereby, we described these species as Ceratocystiopsis yantaiensis sp. nov., C. weihaiensis sp. nov., Graphilbum translucens sp. nov. and Sporothrix villosa sp. nov. A majority of the ophiostomatoid fungi recovered in this study were novel species. This suggests that the forests in China harbour an assortment of undescribed ophiostomatoid fungi yet to be discovered.

Bursaphelenchus doui Braasch, Gu, Burgermeister & Zhang, 2005 (Aphelenchida: Parasitaphelenchidae), an associate of Monochamus subfasciatus Bates (Coleoptera: Cerambycidae) and Pinus densiflora Sieb. & Zucc.

Nematology ◽  
2008 ◽  
Vol 10 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Takuya Aikawa ◽  
Noritoshi Maehara ◽  
Kazuma Matsumoto
Keyword(s):  
Pinus Densiflora ◽  
Large Subunit ◽  
Forest Products ◽  
Original Description ◽  
Small Subunit ◽  
Black Pine ◽  
Inoculation Test ◽  
Tracheal System ◽  
Chinese Populations ◽  
Longhorn Beetle

Abstract Bursaphelenchus doui was isolated from a dead Japanese red pine, Pinus densiflora, in Shizuoka, and from the tracheal system of a species of longhorn beetle, Monochamus subfasciatus, collected at Tama Forest Science Garden of Forestry and Forest Products Research Institute, Tokyo, Japan. The Japanese populations of B. doui were compared with the original description of material obtained from coniferous packaging materials imported from Taiwan and Korea to continental China. Additional characters from the Japanese population include a constricted female mucron with a step-like appearance and several morphometric values. The molecular profiles of the Japanese B. doui populations were determined by DNA sequencing and ITS-RFLP profiles and were compared with those of the Taiwanese and Chinese populations of B. doui and other species in the genus. The phylogenetic analysis of the small subunit and large subunit ribosomal DNA indicated that B. doui is clearly included in the xylophilus-group of the genus Bursaphelenchus and may be close to B. conicaudatus and B. luxuriosae. The potential risk of B. doui for pine species is considered to be relatively low because B. doui did not display any pathogenicity to Japanese black pine in an inoculation test.

Morphological variation in Xenoxybelis boulengeri (Procter, 1923) (Serpentes, Xenodontinae, Philodryadini)

Zootaxa ◽  
2008 ◽  
Vol 1743 (1) ◽  
pp. 53 ◽  
Author(s):  
ANA LÚCIA DA COSTA PRUDENTE ◽  
MÁRCIO ANDRÉ AMORIM DA SILVA ◽  
WÁLDIMA ALVES DA ROCHA ◽  
FRANCISCO LUÍS FRANCO
Keyword(s):  
Morphological Variation ◽  
Rare Species ◽  
Original Description ◽  
External Morphology ◽  
Time Analysis ◽  
Morphometric Characters ◽  
Permit Allocation ◽  
Scientific Collections ◽  
First Time ◽  
Molecular Characters

The genus Xenoxybelis contains two species, X. argenteus and X. boulengeri, characterized by semi-arboreal habits and an extremely thin rostral area, classified in the subfamily Xenodontinae (Colubridae). Xenoxybelis boulengeri is considered a rare species and is poorly represented in scientific collections. Its external morphology is known only from the original description and seven other specimens. In this study, twenty additional specimens were examined from the Brazilian states of Acre, Amazonas and Rondônia. Variation in meristic and morphometric characters are described, and new characters are incorporated in a revised diagnosis of the species. Illustrations and a description of the hemipenes of X. boulengeri are presented for the first time. Analysis of morphological and molecular characters permit allocation of Xenoxybelis to the tribe Phylodryadini, together with Ditaxodon, Philodryas, Pseudablabes and Tropidodryas.

Revision of Zorion Pascoe (Coleoptera: Cerambycidae), an endemic genus of New Zealand

Zootaxa ◽  
2005 ◽  
Vol 1066 (1) ◽  
pp. 1 ◽  
Author(s):  
FRANZ-RUDOLF SCHNITZLER ◽  
QIAO WANG
Keyword(s):  
New Species ◽  
New Zealand ◽  
Species Distribution ◽  
Taxonomic Revision ◽  
Original Description ◽  
Biological Information ◽  
Distribution Map ◽  
Endemic Genus ◽  
Longhorn Beetle

The genus Zorion Pascoe (Cerambycidae: Cerambycinae), commonly known as flower longhorn beetle, is endemic to New Zealand where it is widely distributed on the main and some offshore islands. A taxonomic revision of Zorion adults is given, including a description and illustrations of the genus. The previously known species Z. batesi Sharp, Z. guttigerum (Westwood), Z. minutum (Fabricius), and Z. opacum Sharp are redescribed; Z. castum Broun is synonymised with Z.guttigerum. Lectotypes are designated here for Z. guttigerum and Z. opacum. Six new species are described, Z. angustifasciatum sp. nov. from Three Kings Islands, Z. australe sp. nov. from South Island, Z. dugdalei sp. nov. from Poor Knights Island, Z. kaikouraiensis sp. nov. from the Kaikoura region, Z. nonmaculatum sp. nov. from D’Urville Islands, and Z. taranakiensis sp. nov. from the Taranaki region. The species Z. exiguum Gmelin has been excluded from the study because neither the original description nor the holotype could be located. A key to all ten species is included and diagnostic elytral spot patterns are illustrated. Biological information presented is based on publications, collecting records and our observations. A distribution map for all species is included and species distribution is discussed in relation to New Zealand’s biogeographical history.

Ophiostomatoid fungi isolated from Pinus radiata logs imported from New Zealand to Korea

2005 ◽  
Vol 83 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Gyu-Hyeok Kim ◽  
Jae-Jin Kim ◽  
Young Woon Lim ◽  
Colette Breuil
Keyword(s):  
New Zealand ◽  
Pinus Radiata ◽  
Pathogenic Fungi ◽  
Radiata Pine ◽  
Ophiostomatoid Fungi ◽  
Ophiostoma Piceae ◽  
Leptographium Procerum ◽  
Native Fungi ◽  
Ophiostoma Floccosum ◽  
Ophiostoma Piliferum

Ophiostomatoid fungi discolor lumber, logs, and tree sapwood. Stained wood has a lower market value and can be refused by importing customers because such products can potentially carry pathogenic fungi. Little information is available on the ophiostomatoid fungi that colonize Pinus radiata D. Don (radiata pine) logs imported from New Zealand into Korea. In this work, we attempted to identify the native and non-native fungi colonizing wood imported into Korea. At least 12 species of ophiostomatoid fungi, including an unknown Ophiostoma sp. A, were identified among the fungi-staining radiata pine. They were Leptographium procerum (Kendr.) Wingf., Leptographium bistatum Kim & Kim, Ophiostoma floccosum Mathiesen, Ophiostoma huntii (Rob.) Hoog & Scheff., Ophiostoma ips (Rumbold) Nannf., Ophiostoma nigrocarpum (David.) Hoog, Ophiostoma piceae (Münch) H. & P. Sydow, Ophiostoma piliferum (Fries) H. & P. Sydow, Ophiostoma quercus (Georgév.) Nannf., Ophiostoma radiaticola Kim et al., and Ophiostoma setosum Uzunovic et al. Of these species, O. floccosum was the dominant species in both logs and boards. We confirmed that many of the sapstain species that we isolated have been previously reported in Korea. However, four species, O. radiatcola, O. setosum, O. huntii, and O. nigrocarpum have not been reported previously in Korea. We also found the new species, L. bistatum, along with an unknown Ophiostoma sp. A.Key words: radiata pine, ophiostomatoid fungi, New Zealand, non-native organisms, β-tubulin gene.

Calling behavior of Tetropium fuscum (Coleoptera: Cerambycidae: Spondylidinae)

2010 ◽  
Vol 142 (3) ◽  
pp. 256-260 ◽  
Author(s):  
Matthew A. Lemay ◽  
Peter J. Silk ◽  
Jon Sweeney
Keyword(s):  
Solid Phase ◽  
Visual Assessment ◽  
The Body ◽  
Calling Behavior ◽  
Gas Chromatography Mass Spectrometry ◽  
Future Research ◽  
Calling Behaviour ◽  
Longhorn Beetle ◽  
Tetropium Fuscum

AbstractWe describe the calling posture of the brown spruce longhorn beetle, Tetropium fuscum (F.), and demonstrate, using solid-phase micro-extraction and gas chromatography – mass spectrometry, that this posture is positively correlated with the release of fuscumol, a male-produced long-range pheromone. The posture is observed only in males and is characterized by an extension of the legs that raises the body entirely off the substrate at an angle of approximately 10°, with the posterior end higher than the head. Characterization of the calling posture is a useful diagnostic tool for future research on the reproductive biology of T. fuscum, since a simple visual assessment can be used instead of chemical analysis to determine when males are emitting fuscumol. In addition, we demonstrate that the presence of conspecific males stimulates calling behaviour in T. fuscum. This study contributes much-needed data on the biology and mating behavior of this invasive wood-boring species.

scholarly journals Assessing the virulence of ophiostomatoid fungi associated with the pine-infesting weevils to scots pine Pinus sylvestris L. seedlings

2013 ◽  
Vol 66 (2) ◽  
pp. 85-94 ◽  
Author(s):  
Robert Jankowiak
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Ophiostomatoid Fungi ◽  
Seedling Mortality ◽  
Fungal Virulence ◽  
Ophiostoma Piceae ◽  
Blue Stain ◽  
Leptographium Procerum ◽  
Ophiostoma Floccosum ◽  
Ophiostoma Piliferum

The pine-infesting weevils are known to be effective vectors of ophiostomatoid fungi. To understand more about fungal virulence of these fungi, inoculation studies were conducted on Scots pine (<em>Pinus sylvestris</em> L.). Two-year-old seedlings were wound-inoculated with one of eleven ophiostomatoid fungi associated with pine-infesting weevils. After 11 weeks, a darkened lesion, extending from the point of inoculation, was observed in all species, except for <em>Ophiostoma</em> cf. <em>abietinum</em> Marm. &amp; Butin, <em>Ophiostoma quercus</em> (Georgev.) Nannf., and <em>Sporothrix inflata </em>de Hoog. Seedling mortality was observed in seedlings inoculated with <em>Leptographium truncatum</em> (M.J. Wingf. &amp; Marasas) M.J. Wingf., <em>Leptographium lundbergii</em> Lagerb. &amp; Melin<em>,</em> <em>Leptographium procerum</em> (W.B. Kendr.) M.J. Wingf., <em>Grosmannia radiaticola</em> (J.J. Kim, Seifert &amp; G.H. Kim) Zipfel, Z.W. de Beer &amp; M.J. Wingf., <em>Ophiostoma floccosum</em> Math.-Käärik,<em> Ophiostoma minus</em> (Hedgc.) Syd. &amp; P. Syd., and <em>Ophiostoma piliferum </em>(Fr.) Syd. &amp; P. Syd. <em>Ophiostoma minus</em> and <em>L. truncatum</em> caused the largest lesions and sapwood blue-stain in Scots pine. <em>Grosmannia radiaticola, Ophiostoma piceae </em>(Münch) Syd. &amp; P. Syd., <em>O. floccosum</em>,<em> O. piliferum,</em> <em>L. lundbergii</em>,and<em> L. procerum </em>produced significantly smaller lesions and sapwood blue-stain than <em>O. minus</em> and <em>L. truncatum</em>, while<em> O</em>. cf. <em>abietinum</em>, <em>O. quercus</em> and <em>S. inflata</em> did not cause any lesions.

scholarly journals Ophiostomatoid fungi isolated from fallen shoots of Scots pine pruned by Tomicus species in Poland

2013 ◽  
Vol 46 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Robert Jankowiak ◽  
Mirosław Kolařík
Keyword(s):  
Scots Pine ◽  
Sequence Comparison ◽  
Taxonomic Status ◽  
Ophiostomatoid Fungi ◽  
Low Frequencies ◽  
Pine Stands ◽  
Ophiostoma Floccosum ◽  
Dna Sequence Comparison ◽  
Scots Pine Stands ◽  
Isolated Species

Ophiostomatoid fungi are known to be associated with <em>Tomicus</em> spp. on <em>Pinus sylvestris</em>. However, very little is actually known about the fungi present in the pine shoots damaged by these insects. The aim of this study was to survey species of <em>Ophiostoma</em> s.lato associated with fallen shoots of Scots pine pruned by <em>Tomicus</em> spp. in Poland. The study was conducted in four pure Scots pine stands in southern and south-western part of the country. Fungi were identified based on morphology and DNA sequence comparison for two gene (the ITS rDNA region and ß–tubulin). In total, 64 isolates obtained, represent seven species of ophiostomatoid fungi, including their asexual states of the genera. Two of them, <em>Ophiostoma</em> sp. 1 and <em>Sporothrix</em> sp. 1 probably represent new taxa. All species were found at very low frequencies. Among them, <em>Ophiostoma minus, Ophiostoma</em> sp. 1 and <em>Sporothrix</em> sp. 1 were the most frequently isolated, with a frequency of 2.0%. Occasionally, isolated species included <em>Leptographium piriforme, Ophiostoma canum, Ophiostoma floccosum</em> and <em>Grosmannia cucullata</em>-like. Association of species of Ophiostoma s. lato with <em>Tomicus</em> spp. and the taxonomic status of two new species are discussed.

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