Enzymes of choline synthesis in diverse plants: variation in phosphobase N-methyltransferase activities

2001 ◽  
Vol 79 (8) ◽  
pp. 897-904 ◽  
Author(s):  
Deborah Lorenzin ◽  
Candace Webb ◽  
Peter S Summers ◽  
Elizabeth A Weretilnyk
Keyword(s):  
Plant Species ◽  
Glycine Betaine ◽  
Spinacia Oleracea ◽  
Fresh Weight ◽  
Leaf Extracts ◽  
Spinacia Oleracea L ◽  
Glycine Max L ◽  
Diverse Plant Species ◽  
Diverse Plant

S-adenosyl-L-methionine dependent phospho-base N-methyltransferases are involved in the sequential methylations of phosphoethanolamine [Formula: see text] phosphomethylethanolamine [Formula: see text] phosphodimethylethanolamine [Formula: see text] phosphocholine. Phosphocholine is a precursor for the ubiquitous phospholipid phosphatidylcholine and for free choline, which can be oxidized to produce the osmoprotectant glycine betaine. Despite the importance of these enzymes to growth and stress tolerance, their activities have been studied in comparatively few plant species. Phospho-base N-methylating activities were assayed in leaf extracts prepared from 17 diverse plant species. All plants tested can perform the first step ( N-methylation of phosphoethanolamine) with in vitro activity rates varying from 0.13 nmol·min–1·g–1 fresh weight for soybean (Glycine max (L.) Merr.) and pea (Pisum sativum L.) to 25 nmol·min–1·g–1 fresh weight for cotton (Gossypium hirsutum L.). Of the plant species surveyed, only soybean and pea showed no capacity to perform the two subsequent N-methylation steps. Exposing plants to prolonged dark periods led to decreased phosphoethanolamine N-methylating activity relative to light-exposed controls with the extent of decrease varying among the species from 30% (Limonium perezii (Stapf) F.T. Hubb) to over 90% (Spinacia oleracea L., Beta vulgaris L., and Amaranthus caudatus L.). Thus, light-responsive properties and levels of phosphobase methyltransferase activities vary among plants with a trend towards higher activities being found in plants that accumulate glycine betaine.Key words: glycine betaine, choline, phosphatidylcholine, phosphocholine.

scholarly journals Insights into Metabolic Engineering of the Biosynthesis of Glycine Betaine and Melatonin to Improve Plant Abiotic Stress Tolerance

2021 ◽  
Author(s):  
Cisse El Hadji Malick ◽  
Miao Ling-Feng ◽  
Li Da-Dong ◽  
Yang Fan
Keyword(s):  
Metabolic Engineering ◽  
Stress Tolerance ◽  
Plant Species ◽  
Glycine Betaine ◽  
Abiotic Stress Tolerance ◽  
Plant Stress ◽  
Enzymatic Reactions ◽  
Plant Stress Tolerance ◽  
Diverse Plant Species ◽  
Diverse Plant

Metabolic engineering in plant can be describe as a tool using molecular biological technologies which promotes enzymatic reactions that can enhance the biosynthesis of existing compounds such as glycine betaine (GB) in plant species that are able to accumulate GB, or produce news compounds like GB in non-accumulators plants. Moreover we can include to these definition, the mediation in the degradation of diverse compounds in plant organism. For decades, one of the most popular ideas in metabolic engineering literature is the idea that the improvement of gly betaine or melatonin accumulation in plant under environmental stress can be the main window to ameliorate stress tolerance in diverse plant species. A challenging problem in this domain is the integration of different molecular technologies like transgenesis, enzyme kinetics, promoter analysis, biochemistry and genetics, protein sorting, cloning or comparative physiology to reach that objective. A large number of approaches have been developed over the last few decades in metabolic engineering to overcome this problem. Therefore, we examine some previous work and propose some understanding about the use of metabolic engineering in plant stress tolerance. Moreover, this chapter will focus on melatonin (Hormone) and gly betaine (Osmolyte) biosynthesis pathways in engineering stress resistance.

scholarly journals Phytophthora Diversity in Pennsylvania Nurseries and Greenhouses Inferred from Clinical Samples Collected over Four Decades

2020 ◽  
Vol 8 (7) ◽  
pp. 1056
Author(s):  
Cody Molnar ◽  
Ekaterina Nikolaeva ◽  
Seonghwan Kim ◽  
Tracey Olson ◽  
Devin Bily ◽  
...  
Keyword(s):  
Plant Species ◽  
Morphological Characteristics ◽  
Phytophthora Species ◽  
Clinical Samples ◽  
Department Of Agriculture ◽  
Distinct Subgroup ◽  
Exotic Pathogens ◽  
Diverse Plant Species ◽  
Insight Into ◽  
Diverse Plant

The increasing movement of exotic pathogens calls for systematic surveillance so that newly introduced pathogens can be recognized and dealt with early. A resource crucial for recognizing such pathogens is knowledge about the spatial and temporal diversity of endemic pathogens. Here, we report an effort to build this resource for Pennsylvania (PA) by characterizing the identity and distribution of Phytophthora species isolated from diverse plant species in PA nurseries and greenhouses. We identified 1137 Phytophthora isolates cultured from clinical samples of >150 plant species submitted to the PA Department of Agriculture for diagnosis from 1975 to 2019 using sequences of one or more loci and morphological characteristics. The three most commonly received plants were Abies, Rhododendron, and Pseudotsuga. Thirty-six Phytophthora species identified represent all clades, except 3 and 10, and included a distinct subgroup of a known species and a prospective new species. Prominent pathogenic species such as P. cactorum, P. cinnamomi, P. nicotianae, P. drechsleri, P. pini, P. plurivora, and P. sp. kelmania have been found consistently since 1975. One isolate cultured from Juniperus horizontalis roots did not correspond to any known species, and several other isolates also show considerable genetic variation from any authentic species or isolate. Some species were isolated from never-before-documented plants, suggesting that their host range is larger than previously thought. This survey only provides a coarse picture of historical patterns of Phytophthora encounters in PA nurseries and greenhouses because the isolation of Phytophthora was not designed for a systematic survey. However, its extensive temporal and plant coverage offers a unique insight into the association of Phytophthora with diverse plants in nurseries and greenhouses.

scholarly journals First Report of Tomato chlorotic spot virus in Hoya wayetii and Schlumbergera truncata

2015 ◽  
Vol 16 (1) ◽  
pp. 29-30 ◽  
Author(s):  
Carlye A. Baker ◽  
Scott Adkins
Keyword(s):  
Plant Species ◽  
Vegetable Crops ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
Production Space ◽  
Related Plant ◽  
Diverse Plant Species ◽  
Diverse Plant

To the best of our knowledge, this is the first report of TCSV infection of H. wayetii and S. truncata from any location, although other tospoviruses are known to infect these and related plant species. The identification of these two diverse plant species as the first reported natural ornamental hosts of TCSV has implications for TCSV epidemiology and management in ornamental and vegetable crops, which frequently share production space. Accepted by publication 15 January 2015. Published 25 February 2015.

scholarly journals Site-specific serine phosphorylation of spinach leaf sucrose-phosphate synthase

1992 ◽  
Vol 283 (3) ◽  
pp. 877-882 ◽  
Author(s):  
J L A Huber ◽  
S C Huber
Keyword(s):  
Okadaic Acid ◽  
Spinacia Oleracea ◽  
Sucrose Phosphate Synthase ◽  
Serine Phosphorylation ◽  
Spinacia Oleracea L ◽  
Spinach Leaf ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

We recently reported [Huber, Huber & Nielsen (1989) Arch. Biochem. Biophys. 270, 681-690] that spinach (Spinacia oleracea L.) sucrose-phosphate synthase (SPS; EC 2.4.1.14) was phosphorylated in vivo when leaves were fed [32P]Pi. In vitro the enzyme was phosphorylated and inactivated by using [gamma-32P]ATP. We now report that SPS is phosphorylated both in vivo and in vitro on serine residues. The protein is phosphorylated at multiple sites both in vivo and in vitro as indicated by two-dimensional peptide maps of the immunopurified SPS protein. After being fed with radiolabel, leaves were illuminated or given mannose (which activates the enzyme), in the presence or absence of okadaic acid. Feeding okadaic acid to leaves decreased the SPS activation state in the dark and light and in leaves fed mannose. Across all the treatments, the activation state of SPS in situ was inversely related to the labelling of two phosphopeptides (designated phosphopeptides 5 and 7). These two phosphopeptides are phosphorylated when SPS is inactivated in vitro with [gamma-32P]ATP, and thus are designated as regulatory (inhibitory) sites [Huber & Huber (1991) Biochim. Biophys. Acta 1091, 393-400]. Okadaic acid increased the total 32P-labelling of SPS and in particular increased labelling of the two regulatory sites, which explains the decline in activation state. In the presence of okadaic acid, two cryptic phosphorylation sites became labelled in vivo that were not apparent in the absence of the inhibitor. Overall, the results suggest that light/dark regulation of SPS activity occurs as a result of regulatory serine phosphorylation. Multiple sites are phosphorylated in vivo, but two sites in particular appear to regulate activity and dephosphorylation of these sites in vivo is sensitive to okadaic acid.

scholarly journals Phylogenetic analysis reveals conservation and diversification of micro RNA166 genes among diverse plant species

Genomics ◽  
2014 ◽  
Vol 103 (1) ◽  
pp. 114-121 ◽  
Author(s):  
Suvakanta Barik ◽  
Shabari SarkarDas ◽  
Archita Singh ◽  
Vibhav Gautam ◽  
Pramod Kumar ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Plant Species ◽  
Diverse Plant Species ◽  
Diverse Plant

scholarly journals Green Roof Plant Suitability Analysis for Northern Climates

2013 ◽  
Vol 23 (5) ◽  
pp. 563-574 ◽  
Author(s):  
Katherine L. Vinson ◽  
Youbin Zheng
Keyword(s):  
Plant Species ◽  
Green Roof ◽  
Green Roofs ◽  
Plant Production ◽  
Suitability Analysis ◽  
Production Growth ◽  
Diverse Plant Species ◽  
Extensive Green Roofs ◽  
Different Levels ◽  
Diverse Plant

To select plant species and species combinations for northern climates, mats with different plant species and species combinations were constructed on a green roof plant production farm and later transported and installed on an urban rooftop. There were three treatments: two different planting combinations, which together consisted of 10 diverse plant species [both stonecrop (Sedum) species and nonstonecrop species], and a control, which consisted of 26 stonecrop species used for standard mat production. Growth measurements and observations were made at both sites and special attention was paid to the performance of species during the harvest, transportation, and installation stages, as well as during recovery postinstallation. All species but false rock cress (Aubrieta cultorum) were found to be suitable for extensive green roof applications in northern climates, although there were variations of suitability among the species. Good, mediocre, and poor interactions formed between numerous species, displaying different levels of compatibility. Finally, all species were considered appropriate for a mat production system; species that failed to germinate, species planted postinstallation, the frequently displaced rolling hens and chicks (Jovibarba sobolifera), and false rock cress were exceptions. Overall, many species in this study displayed successful, well-rounded growth. Based on results, species and species combinations were recommended for extensive green roofs in northern climates.

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