Second turkey flock affected by H5N8 HPAI

2017 ◽  
Vol 180 (3) ◽  
pp. 57.2-57
Keyword(s):  
2019 ◽  
Vol 32 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Francesca Profeta ◽  
Cristina E. Di Francesco ◽  
Andrea Di Provvido ◽  
Massimo Scacchia ◽  
Alessandra Alessiani ◽  
...  

Clostridium perfringens type G is one of the pathogens involved in enteric diseases in poultry. NetB, a pore-forming toxin, is considered the main virulence factor responsible for necrotic enteritis during C. perfringens infection. We carried out a field study involving 14 farms to evaluate the occurrence of netB-positive C. perfringens and the impact of infection in Italian poultry flocks. Environmental samples ( n = 117) and 50 carcasses were screened by microbiologic and molecular methods. Microbiologic investigations yielded 82 C. perfringens isolates. DNA was extracted from all samples and screened for α-toxin and NetB encoding genes by real-time PCR. The C. perfringens α-toxin gene was detected in 151 of 167 extracts (90.4%), and 31 of 151 (20.5%) were netB gene positive also. Sixteen isolates from a turkey flock with mild enteric disorders were also netB positive, demonstrating their occurrence not only in broiler but also in turkey flocks. A pulsed-field gel electrophoresis protocol was optimized to evaluate the diversity among isolates and revealed high genetic heterogeneity. The complete NetB toxin-coding gene of 2 C. perfringens isolates from turkey and broiler flocks were analyzed and showed very high relatedness with analogous sequences worldwide.


2012 ◽  
Vol 41 (6) ◽  
pp. 569-577 ◽  
Author(s):  
Léni Corrand ◽  
Maxence Delverdier ◽  
Marie-Noëlle Lucas ◽  
Guillaume Croville ◽  
Charles Facon ◽  
...  

Nature ◽  
1964 ◽  
Vol 204 (4962) ◽  
pp. 1003-1003 ◽  
Author(s):  
R. YAMAMOTO ◽  
C. H. BIGLAND
Keyword(s):  

1992 ◽  
Vol 36 (3) ◽  
pp. 760 ◽  
Author(s):  
J. S. Jeffrey ◽  
R. Droual ◽  
C. U. Meteyer ◽  
F. D. Galey ◽  
H. Kinde ◽  
...  
Keyword(s):  

2010 ◽  
Vol 20 (1) ◽  
pp. 134-141
Author(s):  
S.A. Nassif ◽  
Hanan M. Ibrahim ◽  
Zeinab M. Souror ◽  
Arwa H. Elnaggar ◽  
Hayam Farouk ◽  
...  

2012 ◽  
Vol 7 (2) ◽  
pp. e60-e61
Author(s):  
L Corrand ◽  
M. N. Lucas ◽  
J. Y. Douet ◽  
C. L. Etienne ◽  
O Albaric ◽  
...  
Keyword(s):  

2012 ◽  
Vol 141 (6) ◽  
pp. 1286-1297 ◽  
Author(s):  
M. E. ARNOLD ◽  
M. J. SLOMKA ◽  
V. J. COWARD ◽  
S. MAHMOOD ◽  
P. J. RALEIGH ◽  
...  

SUMMARYThe purpose of this study was to determine whether pooling avian influenza (AI)-positive swabs with negative swabs has a detrimental effect on the sensitivity of AI real-time reverse transcription–polymerase chain reactions (rRT–PCRs). Cloacal and buccal swabs were sampled daily from 12 turkeys infected with A/goose/England/07(H2N2). For half the turkeys, each swab was mixed with four swabs from known AI-negative turkeys, and for the other half the swabs were tested individually. Bayesian modelling was used to (i) determine whether pooling the positive swabs compromised the cycle threshold (Ct) value obtained from the rRT–PCRs, and (ii) estimate the likelihood of detection of an H2N2 infected turkey flock via rRT–PCR for pooled and individually tested swabs (cloacal and buccal)vs. the number of days post-infection of the flock. Results indicated that there was no significant effect of compromising AI rRT–PCR sensitivity by pooling a weak positive swab with negative swabs on the Ctvalues which were obtained. Pooled sampling was able to widen the detection window compared to individual sampling, for the same number of rRT–PCR tests. This indicates that pooled sampling would be an effective method of reducing the number of tests to be performed to determine flock status during an AI outbreak and for surveillance.


2012 ◽  
Vol 56 (2) ◽  
pp. 427-431 ◽  
Author(s):  
L. Corrand ◽  
M. N. Lucas ◽  
J. Y. Douet ◽  
C. L. Etienne ◽  
O. Albaric ◽  
...  
Keyword(s):  

2008 ◽  
Vol 71 (9) ◽  
pp. 1791-1796 ◽  
Author(s):  
S. L. WRIGHT ◽  
D. K. CARVER ◽  
R. M. SILETZKY ◽  
S. ROMINE ◽  
W. E. M. MORROW ◽  
...  

Eastern North Carolina is a major contributor to both turkey and swine production in the United States. In this region, turkeys and swine are frequently grown in close proximity and by common growers. To further characterize colonization of turkeys and swine with Campylobacter in such a setting, we investigated the prevalence of thermophilic campylobacters in eight paired operations involving turkey farms in close proximity to finishing swine farms. All 15 surveyed flocks and 15 herds were Campylobacter positive at one or more sampling times. Campylobacter was isolated from 1,310 (87%) of the 1,512 turkey samples and 1,116 (77%) of the 1,448 swine samples. Most (>99%) campylobacters from swine samples were Campylobacter coli, found in 59 to 97% of the samples from the different herds. Both Campylobacter jejuni and C. coli were recovered from the turkey flocks (overall prevalences of 52 and 35%, respectively). Prevalence among flocks ranged from 31 to 86% for C. jejuni and 0 to 67% for C. coli, and both species were recovered from most flocks. Relative prevalence of C. coli was higher in young birds (brooders), whereas C. jejuni predominated in grow-out birds (P < 0.0001). The prevalence of C. coli in a swine herd was generally not a good predictor for prevalence of this species in the corresponding turkey flock. These findings indicate that even though turkeys and swine grown in proximity to each other were commonly colonized with thermophilic campylobacters, the relative prevalences of C. jejuni and C. coli appear to be host associated.


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