Immuno-dot blot as a rapid diagnostic method for detection of chlamydial infection in koalas (Phasolarctos cinereus)

1993 ◽  
Vol 133 (6) ◽  
pp. 136-141 ◽  
Author(s):  
A. Girjes ◽  
W. Ellis ◽  
M. Lavin ◽  
F. Carrick
Keyword(s):  
Chlamydial Infection ◽  
Diagnostic Method ◽  
Dot Blot ◽  
Rapid Diagnostic Method

scholarly journals Comparative Study of Newer and Established Methods of Diagnosing Coccidioidal Meningitis

2020 ◽  
Vol 6 (3) ◽  
pp. 125
Author(s):  
David A. Stevens ◽  
Marife Martinez ◽  
Gabriele Sass ◽  
Demosthenes Pappagianis ◽  
Brian Doherty ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Comparative Study ◽  
Early Treatment ◽  
Diagnostic Method ◽  
Complement Fixation ◽  
Optimal Test ◽  
Patient Status ◽  
Lateral Flow Assays ◽  
Rapid Diagnostic Method ◽  
Positive Results

Meningitis is the most devastating form of coccidioidomycosis. A convenient, rapid diagnostic method could result in early treatment and avoid many meningitis complications. We studied cerebrospinal fluid (CSF) samples in patients with documented coccidioidal meningitis, and controls, with complement fixation (CF), immunodiffusion (ID) (the “classical” assays), lateral flow assays (LFA; one-strip and two-strip), and two enzyme immunoassays (EIA). The two-strip LFA and EIAs not only enabled separate testing for IgG and IgM antibodies separately, but also could aggregate results for each method. CF with ID or the aggregate use of IgG and IgM tests were considered optimal test uses. LFAs and EIAs were evaluated at 1:21 and 1:441 dilutions of specimens. All assays were compared to true patient status. With 49 patient specimens and 40 controls, this is the largest comparative study of CSF coccidioidal diagnostics. Sensitivity of these tests ranged from 71–95% and specificity 90–100%. IgM assays were less sensitive. Assays at 1:441 were similarly specific but less sensitive, suggesting that serial dilutions of samples could result in assays yielding titers. Agreement of positive results on cases was 87–100%. When kits are available, hospital laboratories in endemic areas can perform testing. LFA assays do not require a laboratory, are simple to use, and give rapid results, potentially even at the bedside.

Intraoperative sentinel lymph node assessment in breast cancer: a comparison of rapid diagnostic method based on CK19 mRNA expression and imprint cytology

2014 ◽  
Vol 84 (10) ◽  
pp. 730-734 ◽  
Author(s):  
Nirmala Pathmanathan ◽  
Jasveen Renthawa ◽  
James R. French ◽  
Elizabeth Edstrom-Elder ◽  
Geoffrey Hall ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Mrna Expression ◽  
Diagnostic Method ◽  
Imprint Cytology ◽  
Ck19 Mrna ◽  
Lymph Node Assessment ◽  
Rapid Diagnostic Method

scholarly journals Rapid discrimination of the phenotypic variants of von Willebrand disease

Blood ◽  
2016 ◽  
Vol 127 (20) ◽  
pp. 2472-2480 ◽  
Author(s):  
Jonathan C. Roberts ◽  
Patti A. Morateck ◽  
Pamela A. Christopherson ◽  
Ke Yan ◽  
Raymond G. Hoffmann ◽  
...  
Keyword(s):  
Diagnostic Method ◽  
Von Willebrand Disease ◽  
Activity Assay ◽  
Key Points ◽  
Rapid Diagnostic Method ◽  
Rapid Discrimination ◽  
Von Willebrand

Key Points A novel ELISA-based VWF multiplex activity assay assigns VWD phenotype among a cohort of type 1 and 2 VWD with an overall accuracy of >88%. This assay shows correlation with traditional quantitative clinical VWF assays and may provide a rapid diagnostic method for variant VWD.

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