Commercial porcine reproductive and respiratory syndrome virus (PRRSV)-2 modified live virus vaccine against heterologous single and dual Korean PRRSV-1 and PRRSV-2 challenge

2018 ◽  
Vol 182 (17) ◽  
pp. 485-485 ◽  
Author(s):  
Jiwoon Jeong ◽  
Seeun Kim ◽  
Changhoon Park ◽  
Kee Hwan Park ◽  
Ikjae Kang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Virus Vaccine ◽  
Interferon Γ ◽  
Growing Pigs ◽  
Respiratory Syndrome Virus ◽  
Live Virus ◽  
Lung Lesions ◽  
Viral Loads ◽  
Live Virus Vaccine ◽  
Ifn Γ

This study evaluated porcine reproductive and respiratory syndrome virus (PRRSV)-2 modified live virus (MLV) vaccine against heterologous single and dual challenge of Korean PRRSV-1 and PRRSV-2. Pigs were administered PRRSV-2 MLV vaccine intramuscularly at 21 days of age and inoculated intranasally with both genotypes at 56 days of age. Vaccination of pigs with PRRSV-2 MLV vaccine resulted in reduction of viral loads of both PRRSV-1 and PRRSV-2 after heterologous single and dual challenge with PRRSV-1 and PRRSV-2. In addition, pigs vaccinated with PRRSV-2 MLV vaccine exhibited higher frequencies of PRRSV-1 and PRRSV-2 specific interferon-γ secreting cells (IFN-γ-SC) and showed a significant reduction in lung lesions and PRRSV nucleic acid within the lung lesions after single and dual challenge compared with unvaccinated challenged pigs. Taken together these results demonstrated that vaccination of pigs with PRRSV-2 is efficacious in protecting growing pigs from respiratory disease against heterologous single and dual PRRSV-1 and PRRSV-2 challenge.

scholarly journals Complete Genome Sequence of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain from Two Genotype 1 Modified Live Virus Vaccine Strains

2017 ◽  
Vol 5 (22) ◽  
Author(s):  
Patricia Renson ◽  
Fabrice Touzain ◽  
Arnaud Lebret ◽  
Mireille Le Dimna ◽  
Hélène Quenault ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Virus Vaccine ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Respiratory Syndrome Virus ◽  
Genotype 1 ◽  
Live Virus ◽  
Live Virus Vaccine ◽  
Pig Farm ◽  
Prrsv Strain

ABSTRACT This paper provides information on the complete genome sequence of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated on a French pig farm which was identified as a recombinant strain from two commercial modified live virus vaccine strains of genotype 1 (VP-046BIS and DV strains).

scholarly journals Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus

Vaccine ◽  
2013 ◽  
Vol 31 (41) ◽  
pp. 4508-4515 ◽  
Author(s):  
Xiangdong Li ◽  
Amy Galliher-Beckley ◽  
Hongzhou Huang ◽  
Xiuzhi Sun ◽  
Jishu Shi
Keyword(s):  
Virus Vaccine ◽  
Protective Immunity ◽  
Respiratory Syndrome Virus ◽  
Live Virus ◽  
Live Virus Vaccine

scholarly journals Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant

Vaccine ◽  
2011 ◽  
Vol 29 (23) ◽  
pp. 4058-4066 ◽  
Author(s):  
Varun Dwivedi ◽  
Cordelia Manickam ◽  
Ruthi Patterson ◽  
Katie Dodson ◽  
Michael Murtaugh ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Protective Immunity ◽  
Respiratory Syndrome Virus ◽  
Intranasal Delivery ◽  
Live Virus ◽  
Live Virus Vaccine

scholarly journals Efficacy of a Spanish modified live virus vaccine against homologous porcine reproductive and respiratory syndrome virus infection

2014 ◽  
Vol 4 (3) ◽  
pp. 213
Author(s):  
E. Álvarez ◽  
A. Fernández-García ◽  
C. Prieto ◽  
J. Martínez-Lobo ◽  
I. Simarro ◽  
...  
Keyword(s):  
Virus Infection ◽  
Virus Vaccine ◽  
Respiratory Syndrome Virus ◽  
Live Virus ◽  
Live Virus Vaccine

scholarly journals Efficacy of a Modified Live Virus Vaccine against Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) Administered to 1-Day-Old Piglets in Front of Heterologous PRRSV-1 Challenge

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1342
Author(s):  
Heinrich Kreutzmann ◽  
Sophie Dürlinger ◽  
Christian Knecht ◽  
Michaela Koch ◽  
Marta Cabana ◽  
...  
Keyword(s):  
Field Isolate ◽  
Daily Weight Gain ◽  
Respiratory Syndrome Virus ◽  
Average Daily Weight Gain ◽  
Swine Industry ◽  
Live Virus ◽  
Live Virus Vaccine ◽  
Nasal Swabs ◽  
Ifn Γ ◽  
The Impact

PRRSV is one of the most important viruses in the global swine industry and is often controlled by the use of modified live virus (MLV) vaccines. This study assessed the impact of a PRRSV-1 MLV vaccine applied to 1-day-old piglets challenged on day 28 of life with a PRRSV-1 field isolate (AUT15-33). Twenty-one piglets were vaccinated within 24 h of birth (T02), whereas 20 piglets were left unvaccinated (T01). Necropsy was performed two weeks post-challenge. Comparing the two groups, T02 piglets showed significantly higher (p = 0.017) average daily weight gain. In addition, significantly lower (p < 0.0001) PRRSV RNA loads were measured in serum of T02 piglets at all investigated time points. All T01 piglets were viremic and shed virus in nasal swabs, whereas only 71.4 % and 38.1 % of the T02 group were viremic or shed virus, respectively. Piglets from T02 had significantly higher numbers (p < 0.0001) of IFN-γ producing lymphocytes compared to T01. At necropsy, differences in gross and histologic lung lesions were statistically significant (p = 0.012 and p < 0.0001, respectively) between the two groups. Hence, this MLV vaccine administered to 1-day-old piglets was able to protect piglets against PRRSV infection at weaning.

scholarly journals Comparison of Two Commercial Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Modified Live Vaccines against Heterologous Type 1 and Type 2 PRRSV Challenge in Growing Pigs

2015 ◽  
Vol 22 (6) ◽  
pp. 631-640 ◽  
Author(s):  
Taeyeon Kim ◽  
Changhoon Park ◽  
Kyuhyung Choi ◽  
Jiwoon Jeong ◽  
Ikjae Kang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Interleukin 10 ◽  
Growing Pigs ◽  
Respiratory Syndrome Virus ◽  
Lung Lesions ◽  
Live Vaccines ◽  
Immunological Responses ◽  
Ifn Γ

ABSTRACTThe objective of the present study was to compare the efficacy of two commercial type 1 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against heterologous type 1 and type 2 PRRSV challenge in growing pigs. Vaccination with a type 1 PRRSV vaccine reduced the level of viremia after type 1 PRRSV challenge but did not reduce the level of viremia after the type 2 PRRSV challenge in pigs. Increased levels of interleukin-10 (IL-10) stimulated by type 2 PRRSV coincided with the low numbers of type 2 PRRSV-specific interferon gamma-secreting cells (IFN-γ-SC) in vaccinated pigs after type 2 PRRSV challenge, whereas low levels of IL-10 stimulated by type 1 PRRSV coincided with high numbers of type 1 PRRSV-specific IFN-γ-SC in vaccinated pigs after type 1 PRRSV challenge. Additionally, vaccination with the type 1 PRRSV vaccine effectively reduced the lung lesions and type 1 PRRSV nucleic acids in type 1 PRRSV-challenged pigs but did not reduce lung lesions and type 2 PRRSV nucleic acids in type 2 PRRSV-challenged pigs. There were no significant differences between two commercial type 1 PRRSV vaccines against type 1 and type 2 PRRSV challenge based on virological results, immunological responses, and pathological outcomes. This study demonstrates that vaccinating pigs with the type 1 PRRSV vaccine provides partial protection against respiratory disease with heterologous type 1 PRRSV challenge but no protection with heterologous type 2 PRRSV challenge.

scholarly journals 93 Genomic basis of antibody response to porcine reproductive and respiratory syndrome virus vaccination

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 28-28
Author(s):  
Letícia P Sanglard ◽  
Rohan L Fernando ◽  
Kent Gray ◽  
Daniel C L Linhares ◽  
Jack Dekkers ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Genomic Prediction ◽  
Fixed Effects ◽  
Natural Infection ◽  
Chromosome 7 ◽  
Validation Dataset ◽  
Respiratory Syndrome Virus ◽  
Live Virus ◽  
Live Virus Vaccine ◽  
Genomic Basis

Abstract Antibody (Ab) response to natural infection with porcine reproductive and respiratory syndrome (PRRS) virus has been shown to be highly heritable (~0.40), to be genetically correlated with farrowing performance in PRRSV-infected sows, be controlled by two major QTL on chromosome 7, among others, and have moderate genomic prediction accuracy. However, waiting for PRRS outbreaks to occur to collect data limits the use of Ab response to select for increased PRRS resilience. Thus, we investigated the genomic basis of Ab response to PRRS vaccination with a modified live PRRSV vaccine as a strategy to generate data for this purpose. Nine hundred and six commercial F1 replacement gilts (189±16 days old) were vaccinated with a commercial PRRS modified live virus vaccine. Blood samples were collected 52 days after vaccination to measure Ab response, as sample-to-positive (S/P) ratio using a commercial ELISA, and for SNP genotyping (~50K). BayesC0 was used to estimate heritability for S/P ratio using in a model with contemporary group (CG) as fixed effect and SNP effects as random. Genome-wide association study and genomic prediction for S/P ratio were performed with BayesB (Pi=0.99). For genomic prediction, a three-fold cross-validation was used, in which each CG (n=3) was used as validation dataset. Accuracy of genomic prediction was defined as the correlation between genomic estimated breeding values and phenotypes adjusted for estimates of fixed effects, weighed by the number of individuals in the validation dataset. Heritability of S/P was moderate (0.35±0.04). A QTL was identified on chromosome 7 (25 Mb) explaining ~28% of the genetic variance. Accuracy of genomic prediction was fairly high (0.60±0.15). This is the first study describing the genomic basis of Ab response to PRRS vaccination with modified-live virus vaccine. Additional work is needed to evaluate the genetic correlation of Ab response to vaccination with resilience traits in pigs.

Effects of Low (Modified-live Virus Vaccine) and High (VR-2385)-Virulence Strains of Porcine Reproductive and Respiratory Syndrome Virus on Pulmonary Clearance of Copper Particles in Pigs

1998 ◽  
Vol 35 (5) ◽  
pp. 398-406 ◽  
Author(s):  
R. Thanawongnuwech ◽  
P. G. Halbur ◽  
M. R. Ackermann ◽  
E. L. Thacker ◽  
R. L. Royer
Keyword(s):  
Virus Vaccine ◽  
Copper Phthalocyanine ◽  
Lavage Fluid ◽  
Serum Copper ◽  
Respiratory Syndrome Virus ◽  
Control Group ◽  
Live Virus ◽  
Copper Particles ◽  
Live Virus Vaccine ◽  
Pulmonary Clearance

Seventy-five 3-week-old, crossbred pigs from a herd free of porcine reproductive and respiratory syndrome virus (PRSSV) were randomly assigned to three groups: uninfected controls, pigs inoculated intranasally with RespPRRS/Repro™ modified-live virus vaccine (RespPRRS), and pigs inoculated intranasally with a high-virulence strain of PRRSV (VR-2385). Pigs were intravenously infused with 3% copper phthalocyanine tetrasulfonic acid (0.2 ml/kg) in normal saline 30 minutes before necropsy, which was performed 3, 7, 10, 14, or 28 days postinoculation (DPI) with PRRSV. There were no differences in serum copper concentration in samples collected at 0, 15, or 30 minutes after infusion. Copper concentrations in the lungs of VR-2385-inoculated pigs were significantly lower than levels in the lungs of control and RespPRRS-inoculated pigs at 7, 10, and 14 DPI ( P < 0.05). The greatest difference between the groups was observed at 10 DPI. Liver and spleen copper concentrations were slightly, but not significantly, higher in both PRRSV-infected groups. The percentage of lung affected by grossly visible pneumonia ranged from 0 to 5.6% in the RespPRRS-inoculated group and from 15.2 to 46.4% in the VR-2385-inoculated group, with lesions peaking at 7 and 10 DPI, respectively. PRRSV antigen was demonstrated in both pulmonary alveolar macrophages (PAMs) and pulmonary intravascular macrophages (PIMs) by immunohistochemical methods. Copper particles were demonstrated in the PIMs by light microscopy. PRRSV was isolated from bronchoalveolar lavage fluid of VR-2385-infected pigs from 3 to 28 DPI and from RespPRRS-inoculated pigs from 7 to 28 DPI. No PRRSV, PRRSV antibodies, or PRRSV-induced pneumonia was detected in the control group. These results suggest that 1) PRRSV has a detrimental effect on the uptake of copper particles by PIMs, 2) the severity of PRRSV-induced damage to PIMs differs among strains, and 3) demonstration of PRRSV-induced decreased pulmonary clearance supports the hypothesis that PRRSV infection may make pigs more susceptible to bacterial septicemia.

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